BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0026.p (517 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 226 4e-57 B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 208 1e-51 B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 205 9e-51 B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 161 1e-37 O30654.1 O30654_DICTH Beta-mannanase EC=3.2.1.78 160 4e-37 >B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 Length = 405 Score = 226 bits (576), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 131/366 (35%), Positives = 190/366 (51%), Gaps = 63/366 (17%) Query: 52 PPEAVTPVNPSASQEAADLLDYLVDLGGKGIISGQHDYLESPDEFNNKLKNAAGDYAVLH 111 P + P+NP AS+EA +L ++ D+ G+ I+SGQH+++ E+ +L+ G V+ Sbjct: 24 PESYIAPINPKASEEAINLYRFIQDVQGEYILSGQHNFVGKGSEYTEQLEALTGKSPVVW 83 Query: 112 GYELG-------AINNQSKG---------------------------------------- 124 G + A+ Q G Sbjct: 84 GADFSFTAKGDKAMEFQHAGPANLPAIDVEGVREVVEKLEELGLEWPPPPEMFPELEFLD 143 Query: 125 -TIARQRQAVVASAIKWHEGGGIVAMTFHQNLPGTT------PEWANVHMSLSQEKFDAY 177 TI R++ + A + H G I+ + +H P WA ++ S E+++ Sbjct: 144 ITIEDARESTIEEAKRRHAMGHIITLMWHGCYPTDAYPCDGLSVWAEGNLP-SDEEWNEL 202 Query: 178 VTPGTPQYKSLLASLDEIAVYLGELRDAGVPVLWRPYHEMNGNWFWWGQK---DNFSRLW 234 VT GT ++ +D IAVYL ELRD +PVLWRPYHEMNG WFWWG K D F RLW Sbjct: 203 VTDGTELNEAWKVGMDVIAVYLKELRDDNIPVLWRPYHEMNGEWFWWGHKKGEDGFKRLW 262 Query: 235 DITYDRLVNQHKLNNLLWVWNPNAPN----KWAEPYSAYYPGAAKVDVLAADIYNNDYKQ 290 +TYD L N H+LNNL+WVWN NAP + PY Y+PGA VDVLA D+Y NDYKQ Sbjct: 263 LMTYDYLTNHHELNNLIWVWNANAPRGLPGERGIPYEDYFPGAEYVDVLACDVYRNDYKQ 322 Query: 291 SYYEGLLELANGKPIGIGESGELPDPAILSQTQSKWVYTMTWGKMLTENNNLQKIRSFMT 350 S+++ L+ LA GKP+ +GE GE+P +L + Q +W + MTWG +L +N + I+ Sbjct: 323 SHHDQLVALAEGKPLALGEVGEMPSLEVLKE-QPQWTWMMTWGWILFFSNEAEFIKELYD 381 Query: 351 NQHTVS 356 ++ ++ Sbjct: 382 SEQVLT 387 >B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 506 Score = 208 bits (530), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 125/356 (35%), Positives = 182/356 (51%), Gaps = 35/356 (9%) Query: 21 LFITILPIMFILSLLPWSEARSLSVERNVVIPPEAVTPVNPSASQEAADLLDYLVDLGGK 80 L LP++ L P AR+L PV P AS EA LL+ L DL G+ Sbjct: 4 LRFACLPLLAALCATPTFAARAL--------------PVTPDASPEARALLELLYDLSGR 49 Query: 81 GIISGQHDYLESPDEFNNKLKNAAGDYAVLHGYELG-AINNQSKGTIARQRQAVVASAIK 139 ++GQH+Y D + G + + G A+ S +AR +V I+ Sbjct: 50 HTLTGQHNYPNIKDRNSRFAAQHIGKMPAVFSTDFGHAVAGNSDSYLARPD--IVQECIR 107 Query: 140 WHEGGGIVAMTFH------------QNLPGTTPE-WANVHMSLSQEKFDAYVTPGTPQYK 186 H+ G +V + +H + LPG+ P+ +V L E+F +TPGT + Sbjct: 108 QHQLGALVTICWHAVPPTADEPVTFRPLPGSDPKALKSVQGQLLDEQFRDVLTPGTALHT 167 Query: 187 SLLASLDEIAVYLGELRDAGVPVLWRPYHEMNGNWFWWGQKD---NFSRLWDITYDRLVN 243 A +D IAV+L +L A VPVLWRPYHEMNG WFWWG + + RL+ +DRLVN Sbjct: 168 KWAAQVDAIAVFLKQLEAAHVPVLWRPYHEMNGGWFWWGGRTGEYSTERLYRQIFDRLVN 227 Query: 244 QHKLNNLLWVWNPNAPNKWAEPYSAYYPGAAKVDVLAADIYNNDYKQSYYEGLLELANGK 303 H+L NL+WVW+ + +K + ++PG VDVL D+Y ND+ QSYYE L +L+ GK Sbjct: 228 HHQLKNLVWVWSMDRVSKPGMEHEKFFPGIDYVDVLGLDVYGNDFAQSYYESLEKLSQGK 287 Query: 304 PIGIGESGELPDPAILSQTQSKWVYTMTWGKMLTENNNLQKIRSFMTNQHTVSRQD 359 P+ + E G P IL + Q KW + +TW M+ N + + + M + ++ D Sbjct: 288 PLALAEVGNPPALEILDR-QPKWTFYVTWAGMV-RNTSRKAYATLMADPRILNLDD 341 >B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 Length = 323 Score = 205 bits (522), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 113/306 (36%), Positives = 169/306 (55%), Gaps = 8/306 (2%) Query: 60 NPSASQEAADLLDYLVDLGGKGIISGQHDYLESPDEFNNKLKNAAGDYAVLHGYELGAIN 119 NP+AS EA +LL++L + G +SGQH ++ + Y L G + G Sbjct: 3 NPNASPEARELLEFLYSISGDSTLSGQHCVPLVGSTRLQGIEKSVDRYPALFGQDFGFSE 62 Query: 120 NQSKGTIARQRQAVVASAIKWHEGGGIVAMTFHQNLPGTTPE---WANVHMSLSQEKFDA 176 + I RQ +V AI+ HE G ++++ +H P ++ L+ +++ Sbjct: 63 PGTWDGI-NYRQNIVDEAIRRHEEGFVISLMWHAVRPIEEEPVRFRESIQGELTDQEWKD 121 Query: 177 YVTPGTPQYKSLLASLDEIAVYLGELRDAGVPVLWRPYHEMNGNWFWWGQK---DNFSRL 233 +TPGT + + +D IA +L +LRDA VPVLWRPYHEMNG WFWWG+K D + +L Sbjct: 122 LMTPGTEVNERWKSQVDVIAFFLKQLRDADVPVLWRPYHEMNGAWFWWGKKGGEDGYEKL 181 Query: 234 WDITYDRLVNQHKLNNLLWVWNPNAPNKWAEPYSAYYPGAAKVDVLAADIYNNDYKQSYY 293 W + YDRL + HKL+NL+WVWN N + + Y+ YYPG VDVLA D+Y+ + Q+ Y Sbjct: 182 WRMLYDRLTHFHKLDNLVWVWNANEVKQGVDSYATYYPGHDVVDVLATDVYSEAFNQANY 241 Query: 294 EGLLELANGKPIGIGESGELPDPAILSQTQSKWVYTMTWGKMLTENNNLQKIRSFMTNQH 353 E LLELA KPI IGE G++P L + Q +W + M W + + RS ++ Sbjct: 242 EELLELAGDKPIAIGECGKVPTVEKLRE-QPRWAWFMIWERGYEGWDAFGAFRSLFHSEE 300 Query: 354 TVSRQD 359 ++ + Sbjct: 301 VITHDE 306 >B5YAS3.1 B5YAS3_DICT6 Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 469 Score = 161 bits (408), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 110/321 (34%), Positives = 167/321 (52%), Gaps = 35/321 (10%) Query: 59 VNPSASQEAADLLDYLVDLGGKGIISGQHDYLESPDEFNNKLKNAAGDYAVLHGYELGAI 118 V P+ S+EA L+DYLV + GK +SGQ Y D F + N + + G+++ Sbjct: 156 VTPNPSKEAQKLMDYLVSIYGKYTLSGQMGY---KDAF--WIWNITDKFPAICGFDMMDY 210 Query: 119 NNQSKGTIARQRQAVVASAIKWHEGGGIVAMTFHQNLPG---TTP--EWANVHMSLSQEK 173 + A R V AI W GGIV +H N P TP EW + + Sbjct: 211 SPSRVERGASSRD--VEDAIDWWNMGGIVQFQWHWNAPKGLYDTPGKEWWRGFYT-NATS 267 Query: 174 FD---AYVTPGTPQYKSLLASLDEIAVYLGELRDAGVPVLWRPYHEMNGNWFWWGQK--D 228 FD A+ P + YK ++ +D IAV L L++A VP+LWRP HE G WFWWG K + Sbjct: 268 FDIEYAFNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWGAKGPE 327 Query: 229 NFSRLWDITYDRLVNQHKLNNLLWVW-NPNAPN--KWAEPYSAYYPGAAKVDVLAADIY- 284 +LW + +DRLVN HK+NNL+WVW ++P+ KW YPG VD++ ADIY Sbjct: 328 ACKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDALKW-------YPGDEYVDIVGADIYL 380 Query: 285 -NNDYKQS--YYEGLLELANGKP-IGIGESGELPDPAILSQTQSKWVYTMTWGKMLTE-- 338 + DY S + +++L GK + + E+G +PDP ++ + ++ WV+ MTW + Sbjct: 381 KDKDYSPSTGMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFMTWSGFENDPN 440 Query: 339 NNNLQKIRSFMTNQHTVSRQD 359 N + I+ + +++ + Sbjct: 441 KNEISHIKKVFNHPFVITKDE 461 >O30654.1 O30654_DICTH Beta-mannanase EC=3.2.1.78 Length = 398 Score = 160 bits (404), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 108/319 (33%), Positives = 165/319 (51%), Gaps = 31/319 (9%) Query: 59 VNPSASQEAADLLDYLVDLGGKGIISGQHDYLESPDEFNNKLKNAAGDYAVLHGYELGAI 118 V P+ S+EA L+DYLV + GK +SGQ Y D F + N + + G+++ Sbjct: 85 VTPNPSKEAQKLMDYLVSIYGKYTLSGQMGY---KDAF--WIWNITDKFPAICGFDMMDY 139 Query: 119 NNQSKGTIARQRQAVVASAIKWHEGGGIVAMTFHQNLPG---TTP--EWANVHMSLSQEK 173 + A R V AI W GGIV +H N P TP EW + + Sbjct: 140 SPSRVERGASSRD--VEDAIDWWNMGGIVQFQWHWNAPKGLYDTPGKEWWRGFYT-NATS 196 Query: 174 FD---AYVTPGTPQYKSLLASLDEIAVYLGELRDAGVPVLWRPYHEMNGNWFWWGQK--D 228 FD A P + YK ++ +D IAV L L++A VP+LWRP HE G WFWWG K + Sbjct: 197 FDIEYALNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWGAKGPE 256 Query: 229 NFSRLWDITYDRLVNQHKLNNLLWVW-NPNAPNKWAEPYSAYYPGAAKVDVLAADIY--N 285 +LW + +DRLVN HK+NNL+WVW ++P+ +YPG VD++ ADIY + Sbjct: 257 ACKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDA-----LKWYPGDEYVDIVGADIYLKD 311 Query: 286 NDYKQS--YYEGLLELANGKP-IGIGESGELPDPAILSQTQSKWVYTMTWGKMLTE--NN 340 DY S + +++L GK + + E+G +PDP ++ + ++ WV+ MTW + N Sbjct: 312 KDYSPSTGMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFMTWSGFENDPNKN 371 Query: 341 NLQKIRSFMTNQHTVSRQD 359 + I+ + +++ + Sbjct: 372 EISHIKKVFNHPFVITKDE 390 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,468,471,923 Number of extensions: 155850225 Number of successful extensions: 273910 Number of sequences better than 1.0e-05: 346 Number of HSP's gapped: 273408 Number of HSP's successfully gapped: 370 Length of query: 517 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 377 Effective length of database: 1,741,754,103 Effective search space: 656641296831 Effective search space used: 656641296831 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)