BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0024.p (504 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9W4.1 B3K9W4_9BACI Sodium/hydrogen exchanger 637 0.0 Q9K5Q0.1 Q9K5Q0_BACHD Na+/H+ antiporter 478 e-133 A6CL00.1 A6CL00_9BACI Potassium/proton antiporter 466 e-129 Q2B589.1 Q2B589_9BACI Cell volume regulation protein CvrA 463 e-128 Q65JD0.1 Q65JD0_BACLD Putative uncharacterized protein 455 e-126 >B3K9W4.1 B3K9W4_9BACI Sodium/hydrogen exchanger Length = 480 Score = 637 bits (1644), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/475 (68%), Positives = 384/475 (80%), Gaps = 1/475 (0%) Query: 14 MTHLADNIIXXXXXXXXXGVLSTKFSTRFGMPALVLFIAAGMVLSRFVYFNNASLTQVAG 73 M D++I GVL+TKFS+RFGMPALVLFIA GM+LSRF+Y++NA LTQ+ G Sbjct: 1 MPSFVDSMILLCGALLLTGVLTTKFSSRFGMPALVLFIAIGMILSRFIYYDNAQLTQLVG 60 Query: 74 IFALIVILFEGGMQTSIKDIRPIMAPALSLATVGVLLTTAIVGVFARFVLDVPWAESLLF 133 IFALIVILF+GGMQT K+I+P++ PA+SLAT+GVLLTT ++G+ A FVLD+ E LL Sbjct: 61 IFALIVILFQGGMQTDFKEIKPVLGPAISLATIGVLLTTVVIGLCASFVLDISLKEGLLI 120 Query: 134 GAIVGSTDAAAVFSVLGGKNIDKRLTSTLEAESGSNDPMAVFLTVSLIEWIQHPDTAIWS 193 GAIVGSTDAAAVFSVLGG N+ KRL TLEAESGSNDPMAVFLTVSLIEWI+HP ++ Sbjct: 121 GAIVGSTDAAAVFSVLGGTNLKKRLRMTLEAESGSNDPMAVFLTVSLIEWIEHPGLNLFG 180 Query: 194 LLLSFVWEMGIGLVMGIAVGKLAVYLINRINLDSTGLYPVMAIGFAVLTYGLAAMVHSSG 253 L+ SFV EMG+GLV+GI VG LAVY+INRIN DS+GLYPV A+GFAVLTY AA + +SG Sbjct: 181 LIFSFVMEMGLGLVVGILVGMLAVYVINRINFDSSGLYPVTALGFAVLTYAAAAWLGASG 240 Query: 254 LLAVYVIGLTLGNSELMYHRTIMNFNHGFAWMMQIAMFTLLGLLVFPNELADIAWEGLLL 313 LLAVYV+ L LGN+EL Y +TI FN GFAWMMQI MF LLGLLVFPNEL+ + W+GLLL Sbjct: 241 LLAVYVMALLLGNTELTYRKTIQAFNQGFAWMMQIVMFVLLGLLVFPNELSKVVWQGLLL 300 Query: 314 SVILMLIARPVGVFLSLLFARFSTREKTLISWAGLRGAVPIVLATYPLLAGLPQGRLXXX 373 S+IL+ IARP+GVF SLLFA+F+ REK LISWAGLRGAVPIVLATYP+LAG+ QG L Sbjct: 301 SLILIFIARPIGVFSSLLFAKFAVREKILISWAGLRGAVPIVLATYPVLAGIRQGELFFN 360 Query: 374 XXXXXXLTSAVIQGTTISPLASRLRLVGKDDAQ-PSLMELVALGKTDSEFNHIAIDRHMP 432 LTSAVIQGTTISPL RL LV KD A+ PSL+EL+ALGKTDSE +H+ +D MP Sbjct: 361 VVFFVVLTSAVIQGTTISPLTQRLALVEKDKAKAPSLLELMALGKTDSEISHVLVDGDMP 420 Query: 433 IAGMQIAQIGLPEDILFTAIIRNKSIVTPHGSTVIEPGDTVYVLSPKSKRDEMRA 487 I G +I Q+ LP+DILFTAIIR K I+TP G+TVIE GDT+YV++PK KR EM++ Sbjct: 421 IVGKEIQQLNLPDDILFTAIIRKKRIITPRGNTVIEAGDTLYVMNPKQKRKEMKS 475 >Q9K5Q0.1 Q9K5Q0_BACHD Na+/H+ antiporter Length = 490 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 249/489 (50%), Positives = 337/489 (68%), Gaps = 9/489 (1%) Query: 14 MTHLADNIIXXXXXXXXXGVLSTKFSTRFGMPALVLFIAAGMVLSR----FVYFN--NAS 67 M D II GV++ KFSTR G+PALVLFI GM++ ++F+ A Sbjct: 1 MDFHTDTIILLAGLLFIVGVITAKFSTRLGVPALVLFILVGMLVGSDGLGLIHFDFSQAH 60 Query: 68 LTQVAGIFALIVILFEGGMQTSIKDIRPIMAPALSLATVGVLLTTAIVGVFARFVLDVPW 127 Q+ GIFAL++ILFEGG+QT K ++ + P+LSLAT GVL+T+ +V V A++VL V W Sbjct: 61 FAQLIGIFALVIILFEGGLQTKWKTVQSVAVPSLSLATFGVLITSTLVAVAAKYVLGVSW 120 Query: 128 AESLLFGAIVGSTDAAAVFSVLGGKNIDKRLTSTLEAESGSNDPMAVFLTVSLIEWIQHP 187 E+ LFGAIVGSTDAAAVF+VL +NI RL +TLEAESG+NDPMAVFLT+SLIE I Sbjct: 121 LEAYLFGAIVGSTDAAAVFAVLKEQNIKDRLATTLEAESGTNDPMAVFLTLSLIELITIG 180 Query: 188 DTAIWSLLLSFVWEMGIGLVMGIAVGKLAVYLINRINLDSTGLYPVMAIGFAVLTYGLAA 247 L SF+W+MG GL++G +GKL Y IN+INLDS+GLYPV A+ FA+LTY + A Sbjct: 181 SMNPLHLFGSFLWQMGAGLILGFGLGKLGSYSINKINLDSSGLYPVFALAFALLTYSVTA 240 Query: 248 MVHSSGLLAVYVIGLTLGNSELMYHRTIMNFNHGFAWMMQIAMFTLLGLLVFPNEL--AD 305 M+ +SGLLAVYV L +GN +L Y +I FN GFAWMMQ+ MF +LGLLVFPN+L A Sbjct: 241 MIGASGLLAVYVAALVIGNQDLTYRHSIFRFNEGFAWMMQMLMFIILGLLVFPNQLFEAS 300 Query: 306 IAWEGLLLSVILMLIARPVGVFLSLLFARFSTREKTLISWAGLRGAVPIVLATYPLLAGL 365 I +G+LLS+ILML+ARPV VFL+ + RF +EK +SWAGLRGAVPIVLAT+P+ AGL Sbjct: 301 IFIKGILLSLILMLVARPVAVFLTTIKMRFDWKEKLFLSWAGLRGAVPIVLATFPMTAGL 360 Query: 366 PQGRLXXXXXXXXXLTSAVIQGTTISPLASRLRLVGKDDAQP-SLMELVALGKTDSEFNH 424 L LTSA+IQG+TI+ A++L+L G P +ELV++GK ++E Sbjct: 361 ENSPLFFNVVFFVVLTSALIQGSTIAHFANKLQLTGPQKTTPLHSLELVSIGKANAEIVE 420 Query: 425 IAIDRHMPIAGMQIAQIGLPEDILFTAIIRNKSIVTPHGSTVIEPGDTVYVLSPKSKRDE 484 + + + P+ G + +I LP+D+L AIIR++ ++TP+G T I+ D +YVL K K+ + Sbjct: 421 LEMKQDTPVIGKTLEEINLPKDVLVNAIIRDEQLITPYGKTTIQADDILYVLVSKKKKRQ 480 Query: 485 MRAFFRSGK 493 ++ F++ + Sbjct: 481 LKELFQAKE 489 >A6CL00.1 A6CL00_9BACI Potassium/proton antiporter Length = 490 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 244/478 (51%), Positives = 327/478 (68%), Gaps = 11/478 (2%) Query: 18 ADNIIXXXXXXXXXGVLSTKFSTRFGMPALVLFIAAGMVLSR----FVYFNNASLTQVAG 73 +D I GV++TKFSTR G+PALVLFI GM+ F+YF+NA LTQ+ G Sbjct: 8 SDYFILLTALLLCAGVITTKFSTRLGVPALVLFILVGMITGSDGLGFIYFDNARLTQMIG 67 Query: 74 IFALIVILFEGGMQTSIKDIRPIMAPALSLATVGVLLTTAIVGVFARFVLDVPWAESLLF 133 + ALI+ILFEGG+QT I ++ + PALSLAT GVL+TT +V V A+ +L V W E LF Sbjct: 68 VLALIIILFEGGLQTKISTVKAVAKPALSLATAGVLITTTVVAVAAKLILGVSWLEGFLF 127 Query: 134 GAIVGSTDAAAVFSVLGGKNIDKRLTSTLEAESGSNDPMAVFLTVSLIEWIQHPDTAIWS 193 G+IVGSTDAAAVF+VL GKN+ +L S LE ESG+NDPMAVFLT+ IE + + Sbjct: 128 GSIVGSTDAAAVFAVLKGKNVKGKLGSILEVESGTNDPMAVFLTIFFIELLTVDKPVYFL 187 Query: 194 LLLSFVWEMGIGLVMGIAVGKLAVYLINRINLDSTGLYPVMAIGFAVLTYGLAAMVHSSG 253 L SF W+MGIGL++GI +GK + INRINLDS+GLYPV + FA+L Y + ++++SG Sbjct: 188 LAGSFFWQMGIGLLLGIIIGKAGSFFINRINLDSSGLYPVFTMAFALLAYSITDLLNASG 247 Query: 254 LLAVYVIGLTLGNSELMYHRTIMNFNHGFAWMMQIAMFTLLGLLVFPNELADIAWE---- 309 LLAVYV + +GN EL Y +I FN GFAWMMQI MF +LGLLVFP +L WE Sbjct: 248 LLAVYVAAMVIGNKELTYRHSIFKFNEGFAWMMQILMFVILGLLVFPAQL--FTWEVVGK 305 Query: 310 GLLLSVILMLIARPVGVFLSLLFARFSTREKTLISWAGLRGAVPIVLATYPLLAGLPQGR 369 GLLLS IL+ +ARP+ VF+S++ + EK +SWAGLRGAVPIVLAT+PL+AGL + Sbjct: 306 GLLLSAILIFVARPIAVFVSMIKMNYQLNEKIFLSWAGLRGAVPIVLATFPLIAGLENSQ 365 Query: 370 LXXXXXXXXXLTSAVIQGTTISPLASRLRLVGKDDAQPS-LMELVALGKTDSEFNHIAID 428 + LTSA+IQG+TIS A++L L G A+P+ +EL+++GK D+E ++ Sbjct: 366 MIFNVVFFVVLTSALIQGSTISKFAAKLGLTGSTKAEPAHSLELISIGKADAEILEAEVN 425 Query: 429 RHMPIAGMQIAQIGLPEDILFTAIIRNKSIVTPHGSTVIEPGDTVYVLSPKSKRDEMR 486 MPI G +A I P+D+L A+IR +VTP+G TVI+ GD +Y+L ++ + E++ Sbjct: 426 EEMPIVGKSLADISFPKDVLINAVIRGADLVTPYGETVIQKGDILYILVSRNSKRELK 483 >Q2B589.1 Q2B589_9BACI Cell volume regulation protein CvrA Length = 498 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 248/471 (52%), Positives = 327/471 (69%), Gaps = 7/471 (1%) Query: 19 DNIIXXXXXXXXXGVLSTKFSTRFGMPALVLFIAAGMVLSR----FVYFNNASLTQVAGI 74 D I GVL+TKFS+R G+P+LVLF+A GM++ +YF+NAS Q+ G+ Sbjct: 8 DTFILLAAILFITGVLTTKFSSRLGVPSLVLFMAVGMLMGSDVLGIIYFDNASTAQMIGV 67 Query: 75 FALIVILFEGGMQTSIKDIRPIMAPALSLATVGVLLTTAIVGVFARFVLDVPWAESLLFG 134 ALI+ILFEGG+QT K +RP++ P+LSLAT+GV+LT+ IV A+ +L W ES+LFG Sbjct: 68 LALIIILFEGGLQTKWKTLRPVVLPSLSLATLGVILTSGIVAAAAKLILGTGWLESILFG 127 Query: 135 AIVGSTDAAAVFSVLGGKNIDKRLTSTLEAESGSNDPMAVFLTVSLIEWIQHPDTAIWSL 194 AIVGSTDAAAVF+VL KNID+R+ +TLEAESGSNDPMAVFLTV++IE I P+ +IWSL Sbjct: 128 AIVGSTDAAAVFAVLKDKNIDERIGATLEAESGSNDPMAVFLTVAMIELITLPEASIWSL 187 Query: 195 LLSFVWEMGIGLVMGIAVGKLAVYLINRINLDSTGLYPVMAIGFAVLTYGLAAMVHSSGL 254 + +F +M IGL+ G+ G+LAV+ +NRINLDS GLYPV A FA+LTYG+ A SGL Sbjct: 188 IGTFFLQMSIGLLFGLIFGRLAVFALNRINLDSGGLYPVFATAFALLTYGITAFASGSGL 247 Query: 255 LAVYVIGLTLGNSELMYHRTIMNFNHGFAWMMQIAMFTLLGLLVFPNEL--ADIAWEGLL 312 LAVY+ + +GN EL Y TI F+ GFAWMMQI MF +LGLLVFP+EL DI +G+ Sbjct: 248 LAVYIAAIIIGNMELAYKHTIFRFSEGFAWMMQIMMFVILGLLVFPSELFSLDIFLKGIA 307 Query: 313 LSVILMLIARPVGVFLSLLFARFSTREKTLISWAGLRGAVPIVLATYPLLAGLPQGRLXX 372 +S IL+LIARP VFLS + + T E +SWAGL+GAVPIVLAT+PLLAG+ + Sbjct: 308 ISAILILIARPAAVFLSTIRMGYKTNELIFLSWAGLKGAVPIVLATFPLLAGVEGSQQIF 367 Query: 373 XXXXXXXLTSAVIQGTTISPLASRLRLVGKDDAQP-SLMELVALGKTDSEFNHIAIDRHM 431 LTS +IQG+TI+ LA+RL+L G P +ELV++GK D+E ++ Sbjct: 368 NVVFFVVLTSCLIQGSTITALAARLKLTGPKKTTPMHSLELVSIGKADAEMIEYEMEDDA 427 Query: 432 PIAGMQIAQIGLPEDILFTAIIRNKSIVTPHGSTVIEPGDTVYVLSPKSKR 482 I G + I P+ L AIIRN ++TP GST+I PGD +Y+L+ + + Sbjct: 428 QIIGQTLIDIPFPKGALVNAIIRNDELITPAGSTIILPGDVLYILASRKSK 478 >Q65JD0.1 Q65JD0_BACLD Putative uncharacterized protein Length = 489 Score = 455 bits (1171), Expect = e-126, Method: Compositional matrix adjust. Identities = 238/452 (52%), Positives = 319/452 (70%), Gaps = 7/452 (1%) Query: 32 GVLSTKFSTRFGMPALVLFIAAGMVLSR----FVYFNNASLTQVAGIFALIVILFEGGMQ 87 GVL+TKFSTR G+PALVLF+ GM++ +YFNN L Q+ GI AL+VILFEGG+Q Sbjct: 20 GVLTTKFSTRLGVPALVLFLIVGMIMGSDGLGIIYFNNPELAQLIGIIALVVILFEGGLQ 79 Query: 88 TSIKDIRPIMAPALSLATVGVLLTTAIVGVFARFVLDVPWAESLLFGAIVGSTDAAAVFS 147 T ++ + P+LSLAT+GVLLTT +V V A+ + +V W E LFGAIVGSTDAAA+F+ Sbjct: 80 TKWSSVKAVAVPSLSLATIGVLLTTCVVAVAAKLIFNVSWMEGFLFGAIVGSTDAAAIFA 139 Query: 148 VLGGKNIDKRLTSTLEAESGSNDPMAVFLTVSLIEWIQHPDTAIWSLLLSFVWEMGIGLV 207 VL G+NI RL+STLEAESG+NDPMA+FLT+SLI+ + ++ + L++SF W+MG+GL+ Sbjct: 140 VLKGQNIRDRLSSTLEAESGTNDPMAMFLTLSLIQLLTADHSSYFMLVISFFWQMGMGLL 199 Query: 208 MGIAVGKLAVYLINRINLDSTGLYPVMAIGFAVLTYGLAAMVHSSGLLAVYVIGLTLGNS 267 +G +G+ A + IN+INLDS+GLYP+ A+ FA+LTY L ++ +SGLLAVY+ L +GN Sbjct: 200 LGYLLGRFASFAINKINLDSSGLYPIFALAFALLTYSLTDVIGASGLLAVYIAALVIGNQ 259 Query: 268 ELMYHRTIMNFNHGFAWMMQIAMFTLLGLLVFPNELA--DIAWEGLLLSVILMLIARPVG 325 +L Y +I FN GFAWMMQI MF +LGLLVFP + DI ++G LLS+IL+ IARPV Sbjct: 260 DLTYRHSIFRFNEGFAWMMQILMFIILGLLVFPAQFLSFDIIFKGFLLSLILIFIARPVA 319 Query: 326 VFLSLLFARFSTREKTLISWAGLRGAVPIVLATYPLLAGLPQGRLXXXXXXXXXLTSAVI 385 VFLS + F+ EK +SWAGL+GAVPIVLAT+P+ GL +L LTSA+I Sbjct: 320 VFLSTIKMGFNFNEKIFLSWAGLKGAVPIVLATFPMTMGLENSQLIFNVVFFVVLTSALI 379 Query: 386 QGTTISPLASRLRLVGKDDAQ-PSLMELVALGKTDSEFNHIAIDRHMPIAGMQIAQIGLP 444 QG+TIS A +L+L G + P +ELV++GK ++E +D I + I P Sbjct: 380 QGSTISVFAEKLKLNGPQKVEAPHSLELVSIGKANAEIIEFEVDDQAAITRQMLKDISFP 439 Query: 445 EDILFTAIIRNKSIVTPHGSTVIEPGDTVYVL 476 +D L AIIRN +VTPHG T I+PGD +Y+L Sbjct: 440 KDALINAIIRNGDLVTPHGETKIKPGDILYIL 471 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,750,955,411 Number of extensions: 110006793 Number of successful extensions: 408873 Number of sequences better than 1.0e-05: 2388 Number of HSP's gapped: 404323 Number of HSP's successfully gapped: 2394 Length of query: 504 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 364 Effective length of database: 1,741,754,103 Effective search space: 633998493492 Effective search space used: 633998493492 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)