BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0018.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9X6.1 B3K9X6_9BACI Helix-turn-helix type 11 domain protein 146 2e-33 A8W027.1 A8W027_9BACI Regulatory protein GntR, HTH 91 9e-17 A5N2K2.1 A5N2K2_CLOK5 Transcriptional regulator 82 5e-14 B9E679.1 B9E679_CLOK1 Putative uncharacterized protein 82 6e-14 C1FL97.1 C1FL97_CLOBJ Putative uncharacterized protein 72 5e-11 >B3K9X6.1 B3K9X6_9BACI Helix-turn-helix type 11 domain protein Length = 132 Score = 146 bits (369), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 69/97 (71%), Positives = 77/97 (79%) Query: 2 ARELAERCGNSERTIYRDMDALSAMHIPIIHLGHGKGYAFIGNFALYPLDWSEEESAAFS 61 ARELAERCG SERTIYRDMDALSAMHIPI H G G+GY FIG+FA+YPL +EEE A Sbjct: 23 ARELAERCGTSERTIYRDMDALSAMHIPITHQGRGRGYTFIGHFAMYPLGLTEEEVQALF 82 Query: 62 QLRGIMDDIKNILPPSFESAYEKVMAAAYKQKAEREE 98 +L +MD IK ILP FESAYEKV+AA YKQ AE+ E Sbjct: 83 KLLNVMDKIKPILPDEFESAYEKVLAAVYKQSAEKAE 119 >A8W027.1 A8W027_9BACI Regulatory protein GntR, HTH Length = 328 Score = 91.3 bits (225), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 78/317 (24%), Positives = 132/317 (41%), Gaps = 40/317 (12%) Query: 2 ARELAERCGNSERTIYRDMDALSAMHIPIIHLGHGKGYAFIGN--------------FAL 47 A ELA+ C ++RTIYRD+ L + I G I N L Sbjct: 27 AGELADHCQATKRTIYRDIHFLQSQGHEIQTQGREGYKLLIPNMQVRSKLTEDEWLSLTL 86 Query: 48 YPLDWSEEESAAFSQLRGIMDDIKNILPPSFESAYEKVMAAAYKQKAEREETMETAKREA 107 YPL S S+ R ++ ++ + A A + A ++T + Sbjct: 87 YPLLSSGIASSDHPYHRHYKTGLEKVMRLASGGAPNPAFAERIRLHARTQDTFQAK---- 142 Query: 108 GQLWMERSGYQDDPPLFLTEVLTAIMKQNSILADY---SENAYAERGIQIDPYCLVPLEN 164 + ++TAI I Y + ++ER +++PY LVP E Sbjct: 143 ----------------VMPAIITAISDNRCIDVRYYAMHRSEWSER--RLEPYYLVPREG 184 Query: 165 RYHLIGFCHRFGIIRTFHLHGFSNVKPTNLWFSKEQ-FDLQAFMEQKWSLDRDSLQVEFK 223 +L+ FC IR F L+ F +V T+ F+ Q F + AF++++WS+ + V+ Sbjct: 185 HLYLVAFCRLRNDIRVFRLNRFEDVHVTDAPFTIPQDFSIDAFLKKRWSIMEEEEPVDIT 244 Query: 224 IRFSEQIIDRIKYEDMFVKPSKVDLQTRCLHFKVAIEQDIGFVRWILNFEEEAEIMEPLY 283 +RFS + ED +V+ K + L V + F+RW+ +F +AE++ P Sbjct: 245 VRFSPDAARYVDEEDFYVETKKYNETDGTLTLTVTVGSRKEFLRWMRSFGPDAEVLSPAS 304 Query: 284 YREMLRHQLEKWLSLYK 300 R+ L + L +Y+ Sbjct: 305 LRQALAAEYRHMLGMYE 321 >A5N2K2.1 A5N2K2_CLOK5 Transcriptional regulator Length = 320 Score = 82.0 bits (201), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 73/306 (23%), Positives = 144/306 (47%), Gaps = 19/306 (6%) Query: 2 ARELAERCGNSERTIYRDMDALSAMHIPIIHLGHGK--GYAFIGNFALYPLDWSEEE-SA 58 A EL+E ++TIYR +++L+ +IP+ H G+ G+ +F + D +EEE A Sbjct: 22 ASELSEMLKVDKKTIYRYINSLNKANIPV-HTRKGRYGGFYIDKSFYMKSADLNEEELKA 80 Query: 59 AFSQLRGIMDDIKNILPPSFESAYEKVMAAAYKQKAEREETMETAKREAGQLWMERSGYQ 118 + + I I +SA K+ + + + T G ++ G Sbjct: 81 LLMASQTFTEKIGFIYEKDLKSAVHKIKSLHINKNMNLDRIDNT-----GGFSIDSIGNV 135 Query: 119 DDPPLFLTEVLTAIMKQNSILADY---SENAYAERGIQIDPYCLVPLENRYHLIGFCHRF 175 ++ + E+ ++ + S+ +Y ++N R ++DPY L+ E +H+IG+CH Sbjct: 136 ENLEHKIYEINFSMSRGRSLNINYFSINKNDLTMR--KVDPYDLIFKEGAWHIIGYCHMK 193 Query: 176 GIIRTFHLHGFSNVK-PTNLWFSKEQFDLQAFMEQKWSLDRDSLQVEFKIRFSEQIIDRI 234 + F L+ ++K N++ F L+ +++ W L +++ IRF ++I I Sbjct: 194 DNVENFQLNRIKSLKVSNNIYMRPRTFSLKDYLDNHWGLFVGD-KIKIVIRFDKEIASFI 252 Query: 235 KYEDMFVKPSKVDLQTRCLHFKVAIEQDIGFVRWILNFEEEAEIMEPLYYREMLRHQLEK 294 ++ +LQ + F + ++ +WIL F + AE++EP RE ++ ++E+ Sbjct: 253 QHTKWHANQIIDELQGGSILFSLYVDDIKDVKQWILGFGKNAEVIEPKVLRENIKLEVEE 312 Query: 295 WLSLYK 300 LYK Sbjct: 313 ---LYK 315 >B9E679.1 B9E679_CLOK1 Putative uncharacterized protein Length = 329 Score = 82.0 bits (201), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 73/306 (23%), Positives = 144/306 (47%), Gaps = 19/306 (6%) Query: 2 ARELAERCGNSERTIYRDMDALSAMHIPIIHLGHGK--GYAFIGNFALYPLDWSEEE-SA 58 A EL+E ++TIYR +++L+ +IP+ H G+ G+ +F + D +EEE A Sbjct: 31 ASELSEMLKVDKKTIYRYINSLNKANIPV-HTRKGRYGGFYIDKSFYMKSADLNEEELKA 89 Query: 59 AFSQLRGIMDDIKNILPPSFESAYEKVMAAAYKQKAEREETMETAKREAGQLWMERSGYQ 118 + + I I +SA K+ + + + T G ++ G Sbjct: 90 LLMASQTFTEKIGFIYEKDLKSAVHKIKSLHINKNMNLDRIDNT-----GGFSIDSIGNV 144 Query: 119 DDPPLFLTEVLTAIMKQNSILADY---SENAYAERGIQIDPYCLVPLENRYHLIGFCHRF 175 ++ + E+ ++ + S+ +Y ++N R ++DPY L+ E +H+IG+CH Sbjct: 145 ENLEHKIYEINFSMSRGRSLNINYFSINKNDLTMR--KVDPYDLIFKEGAWHIIGYCHMK 202 Query: 176 GIIRTFHLHGFSNVK-PTNLWFSKEQFDLQAFMEQKWSLDRDSLQVEFKIRFSEQIIDRI 234 + F L+ ++K N++ F L+ +++ W L +++ IRF ++I I Sbjct: 203 DNVENFQLNRIKSLKVSNNIYMRPRTFSLKDYLDNHWGLFVGD-KIKIVIRFDKEIASFI 261 Query: 235 KYEDMFVKPSKVDLQTRCLHFKVAIEQDIGFVRWILNFEEEAEIMEPLYYREMLRHQLEK 294 ++ +LQ + F + ++ +WIL F + AE++EP RE ++ ++E+ Sbjct: 262 QHTKWHANQIIDELQGGSILFSLYVDDIKDVKQWILGFGKNAEVIEPKVLRENIKLEVEE 321 Query: 295 WLSLYK 300 LYK Sbjct: 322 ---LYK 324 >C1FL97.1 C1FL97_CLOBJ Putative uncharacterized protein Length = 318 Score = 72.4 bits (176), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 64/286 (22%), Positives = 135/286 (47%), Gaps = 14/286 (4%) Query: 2 ARELAERCGNSERTIYRDMDALSAMHIPIIHLGHGK--GYAFIGNFALYPLDWSEEESAA 59 A EL++ ++TIYR +++L+ +IPI H G+ G+ NF + + +E E A Sbjct: 22 AGELSDFLMVDKKTIYRYINSLNLANIPI-HAKKGRYGGFYIDKNFYMKSPELNENEIKA 80 Query: 60 FSQLRGIMDDIKNILPPSFESAYEKVMAAAYKQKAEREETMETAKREAGQLWMERSGYQD 119 I+ + + +E Y+ + + ++ ++ ++ G Sbjct: 81 LLMAGEILTEENGFV---YEKEYKTALGKIKNNLSSKDIELDNI-YNFNDFRIDSLGNNK 136 Query: 120 DPPLFLTEVLTAIMKQNSILADY---SENAYAERGIQIDPYCLVPLENRYHLIGFCHRFG 176 + ++ +IM SI Y + N R +IDPY ++ +++++G+CH Sbjct: 137 ISQDRICKICNSIMNNKSINISYFSINRNEITFR--KIDPYDIMFKYGKWYIVGYCHFNK 194 Query: 177 IIRTFHLHGFSNVKPT-NLWFSKEQFDLQAFMEQKWSLDRDSLQVEFKIRFSEQIIDRIK 235 I F ++ ++K T + + + F + +F+E+ S+ + +V+ +++F++ I D IK Sbjct: 195 YIEIFDINRIKDIKDTRDTFIISKNFSINSFLEKYKSIFTHN-KVKVQLKFNKNIADFIK 253 Query: 236 YEDMFVKPSKVDLQTRCLHFKVAIEQDIGFVRWILNFEEEAEIMEP 281 +V +L+ + FKV +E RWIL F ++ +++EP Sbjct: 254 ENKWYVNEEIKELENGEVLFKVYVENLQEIKRWILGFGKDVQVLEP 299 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,788,556,822 Number of extensions: 71439662 Number of successful extensions: 164876 Number of sequences better than 1.0e-05: 169 Number of HSP's gapped: 164893 Number of HSP's successfully gapped: 169 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)