BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0003.p (633 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q59328.2 Q59328_CLOTM Endo-1,3(4)-beta-glucanase EC=3.2.1.6 235 1e-59 A3DJ79.1 A3DJ79_CLOTH Glycoside hydrolase, family 16 235 1e-59 B4BFD5.1 B4BFD5_CLOTM Glycoside hydrolase family 16 235 1e-59 Q000P7.1 Q000P7_9BACL Endo-beta-1,3-glucanase 220 4e-55 B1D8U2.1 B1D8U2_9BACL Glycoside hydrolase family 16 211 2e-52 >Q59328.2 Q59328_CLOTM Endo-1,3(4)-beta-glucanase EC=3.2.1.6 Length = 1321 Score = 235 bits (600), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 206/353 (58%), Gaps = 13/353 (3%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKVRVAGEGATAIR 312 A GI +G+ TL+ V V+G +++ GGG SIH + K+ VVVN+ VR+ G T++ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVNKT--GVRIVTSGNTSVE 296 Query: 313 RMEIESASIIETD---ADAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 SVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 349 >A3DJ79.1 A3DJ79_CLOTH Glycoside hydrolase, family 16 Length = 1321 Score = 235 bits (600), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 206/353 (58%), Gaps = 13/353 (3%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKVRVAGEGATAIR 312 A GI +G+ TL+ V V+G +++ GGG SIH + K+ VVVN+ VR+ G T++ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVNKT--GVRIVTSGNTSVE 296 Query: 313 RMEIESASIIETDA---DAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 SVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 349 >B4BFD5.1 B4BFD5_CLOTM Glycoside hydrolase family 16 Length = 1321 Score = 235 bits (600), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 206/353 (58%), Gaps = 13/353 (3%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKVRVAGEGATAIR 312 A GI +G+ TL+ V V+G +++ GGG SIH + K+ VVVN+ VR+ G T++ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVNKT--GVRIVTSGNTSVE 296 Query: 313 RMEIESASIIETDA---DAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 SVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 349 >Q000P7.1 Q000P7_9BACL Endo-beta-1,3-glucanase Length = 1792 Score = 220 bits (560), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 123/333 (36%), Positives = 185/333 (55%), Gaps = 5/333 (1%) Query: 52 WAKDSIAKWQGNGVIQGYPDGSFRPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAW 111 WA + KW G G++ G G FRP + V+RAE A +VN LFG DV G W Sbjct: 73 WASRQVGKWAGLGLVNG-SGGQFRPGDTVSRAEFAKLVNALFGFTAKTGGTLGDVAPGKW 131 Query: 112 YAKDLSVAKQAGYYKGFPDNKAKADTQVTRQDAAALLAAVFSLEPRAGAASAS-FTDNAA 170 YA+ +++A QAGY +G+P K + VTRQ+AA + A +F L AA S F D A Sbjct: 132 YAEQVAIALQAGYMEGYPGGLFKPEAAVTRQEAAKIAALLFPLATADSAAVLSGFKDRTA 191 Query: 171 ISSYAKEAVQAL--QGTLQGYPDGSFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTV 228 I +A + + L G L+G+ DG+ RP P+TRAE V ++DRL G+ DG Sbjct: 192 IGGFAVQPLADLVSAGALKGFADGTLRPQQPLTRAEAVVLLDRLAGEIIRQPGSYDGVKS 251 Query: 229 QGNVLVNHTGVILKDNVISGNLYLAPGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAK 288 +L+ ILK + GN+ + G+G GE TL+ ++ G +Y+ GGG HS+H+++AK Sbjct: 252 DSGLLIASADTILKQAEVKGNVLITAGVGEGEVTLDGLSADGTLYVNGGGSHSVHLRNAK 311 Query: 289 LALVVVNRPEGKVRVAGEGATAIRRMEIESASIIETDADAVISEVVTGAGAAGTSIVGKG 348 + VVVN+ G VRV EG++ + M +E+ +++E A ++ + A GT + KG Sbjct: 312 VGKVVVNKSGGPVRVVLEGSSKVGEMSLETGAVVEVGEQAEVASLQVEQSAGGTELNVKG 371 Query: 349 TISRLAVRSGPVVLNGQPLAAGTYI-VKGGAAS 380 T+ L ++ V LNG+ G + V+ G A+ Sbjct: 372 TVGELQTQASGVTLNGETFEQGKVLEVQQGKAA 404 >B1D8U2.1 B1D8U2_9BACL Glycoside hydrolase family 16 Length = 2908 Score = 211 bits (537), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 111/324 (34%), Positives = 184/324 (56%), Gaps = 16/324 (4%) Query: 16 KRFKKGLTTLLTTIMLVSTAAPALAYVRQDDR----------LSTHWAKDSIAKWQGNGV 65 ++ + L +L+ ++++ + P+ + D ++ HWA + W G+ Sbjct: 4 RKKTRRLLSLVMSVVIAVSYVPSAVFAASDTSAVATSAAAPDVAGHWASQQVTDWTSKGL 63 Query: 66 IQGYPDGSFRPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYY 125 + YPDG+F+P+ +TRAE ++N LFG DV A AWYA +S+ K+AG+ Sbjct: 64 VNSYPDGTFKPEQGITRAEFTKLINSLFGFTAKGENSFSDVGANAWYADSVSIGKKAGFI 123 Query: 126 KGFPDNKAKADTQVTRQDAAALLAAVFSLEPRAGAAS--ASFTDNAAISSYAKEAVQALQ 183 G+PD K + +TRQ+AA + A +FSL P AA +F D + + ++A E + L Sbjct: 124 NGYPDGTFKPNAAITRQEAAKMAAGLFSL-PAVTAADPLEAFQDKSQVGAFAVEPLGQLV 182 Query: 184 --GTLQGYPDGSFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVIL 241 G L+G+P+G+ +P +TRAE V ++ RL Y A T + GN LVN GV + Sbjct: 183 SGGYLKGFPNGTVQPLKSITRAEAVVLLSRLAGEVYVKAQTYKDQKLAGNGLVNLDGVTI 242 Query: 242 KDNVISGNLYLAPGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKV 301 +++ I GNLYL GIG G+ TL+ V G ++I+GGG HSIH+ + + ++V++ EG V Sbjct: 243 ENSAIGGNLYLTAGIGEGDFTLKNTEVNGTIFISGGGVHSIHLNNVHVPAIIVDKKEGPV 302 Query: 302 RVAGEGATAIRRMEIESASIIETD 325 RV +G + + M++ S +++ET+ Sbjct: 303 RVIVDG-SEVGTMDLNSDTVLETN 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,647,849,510 Number of extensions: 153614404 Number of successful extensions: 334909 Number of sequences better than 1.0e-05: 880 Number of HSP's gapped: 332619 Number of HSP's successfully gapped: 949 Length of query: 633 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 491 Effective length of database: 1,722,912,073 Effective search space: 845949827843 Effective search space used: 845949827843 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 135 (56.6 bits)