BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0002.p (783 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2ACI1.1 Q2ACI1_9BACI Mannanase 462 e-128 Q6JAM5.1 Q6JAM5_CELFI Man26A (Fragment) 191 2e-46 B5HUY5.1 B5HUY5_9ACTO Mannanase 171 4e-40 Q9XCV5.1 Q9XCV5_CELFI Man26A 137 5e-30 C4IK30.1 C4IK30_CLOBU Beta-mannosidase 115 1e-23 >Q2ACI1.1 Q2ACI1_9BACI Mannanase Length = 997 Score = 462 bits (1190), Expect = e-128, Method: Compositional matrix adjust. Identities = 269/632 (42%), Positives = 378/632 (59%), Gaps = 31/632 (4%) Query: 1 VHNFLYAYSPGGGFGTSGQQYLETYPGDDYVDILGFDSYYN--GEGQSWF-DGVATEAAT 57 V+NFLYA+SP F + QYL TYPGD YVDI G D Y N GQ+ F +G+ + A Sbjct: 248 VNNFLYAFSPNAPFDGNLTQYLRTYPGDQYVDIFGLDQYDNKANAGQATFLNGLTQDLAM 307 Query: 58 LSKIADAKHKIAALTEFGY--QNMKVAGNSTPDFYTRLASALKSNPDAKRMAFMLTWADF 115 +SK+AD K KIAA TE+GY Q GN +YT + A+K +P+A R+A+M TWA+F Sbjct: 308 ISKLADEKGKIAAFTEYGYSPQGFNETGNYL-QWYTAVLEAIKKDPNASRIAYMQTWANF 366 Query: 116 TPSENSYVPYPLASGQ---EHQMMADFKSFFDDPYTLFSGEING-AYTQAVDTAGEQPFM 171 N +VPY +G +H+++ +F F++D Y F E +G Q + T ++PFM Sbjct: 367 GYPTNMFVPYRDVNGNLGGDHELLPNFVEFYEDDYAAFLTEASGWNLYQDISTIEQEPFM 426 Query: 172 HIASPTNQAIVMEGTTLIRVRVLNGSPAKVVYTVGESALETPLVLDPETGFYVAGWSPDA 231 HI +PT + + E T IR RVL+ P+ VV+ V +S E P+ LD + FY+ W+PDA Sbjct: 427 HIVTPTANSQISEAVT-IRARVLHDQPSHVVFEVNDSGEEIPMSLDEDGFFYMGKWTPDA 485 Query: 232 KLNGKIAAVTVKVYGEDGTVLLEQTNSVFVKISEVLLGAYTFDNGIEGVQNNGGYQAAVD 291 +N +TV+ YGE+ + E+T + V++SE+LL YTFD GIEG+QNNG Y ++ Sbjct: 486 AVNHTTVNITVRAYGENQ--VQEETFPLVVRVSEMLLKEYTFDEGIEGIQNNGTYPDTIE 543 Query: 292 -SIEPGQFNGSGVLKVNASGMNPADTWQELKLELKNAKSVIDSVYLTEATRVKLDVWMPV 350 S E NG G LK+N +G+ +DTWQELKLEL N + V L RVK+DV++P Sbjct: 544 TSFEHQVLNGDGKLKINVAGLQASDTWQELKLELTN----LHDVQLGNVNRVKVDVFIPK 599 Query: 351 TAQPENGNASLQAVVQLPPDWNGKYGIGTTQISFADLPKETIDGVEYVHFTPAIDMTNXX 410 A N +A+++ +VQLPPDW+ KYG+ TT+ + +DL ID EYV ID+T+ Sbjct: 600 AAV--NQSATIRGIVQLPPDWDTKYGMTTTEKNLSDLQSVVIDEEEYVEGQITIDLTSPE 657 Query: 411 XXXXXXXXXVSIVGSGLA-SGSIPVYIDNIGLYSTYVETGSDPAVVDDFESYLGSDEALN 469 +S+VG+ + +G+I Y+DNI L E SDPA+VDDFESY+G+D+ L Sbjct: 658 ASAAATGLALSLVGNAIDFTGAI--YVDNIQLIGVSEEEVSDPAIVDDFESYVGNDDLLR 715 Query: 470 TKFVHAGGDATETALDANKHGGSYGMKYTYTL-GASGYAGVTKAL-NVDWSGFNALQFWY 527 +V A G + K G YG+ Y Y+L GA Y G+TK L N DWS +N+LQFW Sbjct: 716 NAWVAANGGIAISLDQEEKSAGDYGLAYEYSLAGAGSYTGITKMLGNRDWSSYNSLQFWM 775 Query: 528 KPDAKGQKLVIQINAGGSTYEYYPDTATEQAALVTAKFSEFVPANGATGT-----LTKTN 582 D GQKLVIQ GG +E YP + LVT F+EF PA + + +T+ Sbjct: 776 NSDGNGQKLVIQAEIGGVHFEAYPSLEANEEGLVTIGFNEFTPAPWESASNLEKLVTEEA 835 Query: 583 LKDVQKFSIYTNAKPD-SMQLTSSMYFDDIKA 613 LK+V K S+Y NA+ + L S+++FD+I+A Sbjct: 836 LKNVTKLSLYINAQDELDSALVSTLFFDEIRA 867 >Q6JAM5.1 Q6JAM5_CELFI Man26A (Fragment) Length = 510 Score = 191 bits (486), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 110/271 (40%), Positives = 164/271 (60%), Gaps = 13/271 (4%) Query: 1 VHNFLYAYSPGGGFGTSGQQYLETYPGDDYVDILGFDSYYNGEG---QSWFDGVATEAAT 57 V NFLYA+SPG F ++YL+TYPGDDYVD+LGFD Y N + + + + + Sbjct: 219 VDNFLYAFSPGASFNGDEEKYLKTYPGDDYVDVLGFDQYDNPNNPGSEGFLNTLVVDLGM 278 Query: 58 LSKIADAKHKIAALTEFGYQNMKVAGNSTPDFYTRLASALKSNPDAKRMAFMLTWADFTP 117 LSK+AD+K KIAALTE+G +K GN ++TR+ A+K++P A+++++M TWA+F Sbjct: 279 LSKLADSKGKIAALTEYGL-GLKTNGNLDTQWFTRVLDAIKADPYARKISYMQTWANFGL 337 Query: 118 SENSYVPYPLASG--QEHQMMADFKSFFDDPYTLFSGEINGAYTQAV-DTAGEQPFMHIA 174 + N +VPY A +H+++ DF +F+ DPY+ FS ++ Y AV +T E+PFMHI Sbjct: 338 NGNLFVPYKNAPNGLGDHELLPDFINFYKDPYSAFSKDVGNIYRGAVPETVAEKPFMHIV 397 Query: 175 SPTNQAIVMEGTTLIRVRVLNGSPAKVVYTVGESALETPLVLDPETGFYVAGWSPDAKLN 234 SP ++++ ++ T I V V+ G P + YTV + A + PLV G+Y G A L Sbjct: 398 SPIDRSLSLQKVTPISVSVIQGKPKDIYYTVNDKAKKYPLV--KGDGYYYEG---SAALK 452 Query: 235 GKIAAVTVKVYGEDGTVLLEQTNSVFVKISE 265 G A + V DGT +QT V++K E Sbjct: 453 GDKATIHVTAEFADGTS-QKQTIKVYLKEPE 482 >B5HUY5.1 B5HUY5_9ACTO Mannanase Length = 786 Score = 171 bits (432), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 102/269 (37%), Positives = 150/269 (55%), Gaps = 18/269 (6%) Query: 1 VHNFLYAYSPGGGFGTSGQQYLETYPGDDYVDILGFDSYYNGEGQS-WFDGVATEAATLS 59 VHN LYAYSP F YL+TYPGD +VD+LGFDSY G + W D V + A + Sbjct: 364 VHNLLYAYSPNSSFAGDPADYLKTYPGDRFVDVLGFDSYDENAGPTPWLDAVVKDLAMVV 423 Query: 60 KIADAKHKIAALTEFGYQNMKVAGNSTPDFYTRLASALKSNPDAKRMAFMLTWADFTPSE 119 ++A+ + K A TEFG +V ++T+LA A++++P A+++ +MLTWA+F ++ Sbjct: 424 RLANERGKAPAFTEFGEGGTEVRNQQ---WFTQLAQAIEADPLARQVTYMLTWANFGGTK 480 Query: 120 NSYVPYPLASGQEHQMMADFKSFFDDPYTLFSGEINGAYTQAVDTAGEQPFMHIASPTNQ 179 +YVPYP H + DF + DPYTLF+ ++ G Y+ PF+H+ +PT++ Sbjct: 481 RAYVPYP-----GHLLFPDFVKYEQDPYTLFAADLRGVYSAHTTAVKNAPFLHLVTPTDR 535 Query: 180 AIVMEGTTLIRVRVLNGSPAKVVYTV-GESALETPLVLDPETGFYVAGWSPDAKL-NGKI 237 V T IRVRV ++V Y+V G A + L LD + GFY W+ D L N + Sbjct: 536 QRVAAPRTTIRVRVTPARASQVTYSVNGGRARK--LCLDTD-GFYSGDWTIDPALRNNRS 592 Query: 238 AAVTVKVYGEDGTVLLEQTNSVFVKISEV 266 +TV DG L T+S V + E+ Sbjct: 593 VTLTVSTR-LDGKTL---TDSAVVLLGEL 617 Score = 77.8 bits (190), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 52/163 (31%), Positives = 72/163 (44%), Gaps = 6/163 (3%) Query: 455 VDDFESYLGSDEALNTKFVHAGGDATETALDANKHGGSYGMKYTYTLGASGYAGVTKALN 514 VDDFE Y + AL+ + H + D +K GSYG+ Y Y S Y G K L+ Sbjct: 625 VDDFEGYAADNTALSQAYTHVNSHTLTLSAD-HKSSGSYGLAYAYDFTGSEYTGAGKPLD 683 Query: 515 VDWSGFNALQFWYKPDAKGQKLVIQINAGGSTYEYYPDTATEQAALVTAKFSEFVPANGA 574 DWS F +L W + D +QI A G + Y + V A FSEF PA Sbjct: 684 ADWSAFTSLALWLRGDGSANGGALQIVADGVDFWYQIPLSDTSGQDVRAPFSEFTPAPWD 743 Query: 575 T----GTLTKTNLKDVQKFSIYTNAKPDSMQLTSSMYFDDIKA 613 T L +L +V F++Y ++Y D+I+A Sbjct: 744 TEHTGAVLDAAHLAEVTAFNLYL-VHGSGAATKGTVYVDNIRA 785 >Q9XCV5.1 Q9XCV5_CELFI Man26A Length = 1010 Score = 137 bits (344), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 79/194 (40%), Positives = 122/194 (62%), Gaps = 7/194 (3%) Query: 1 VHNFLYAYSPGGGFGTSGQQYLETYPGDDYVDILGFDSYYNGEGQSWFDGVATEAATLSK 60 V NFLYA+ PGGGFG + YL TYPGD +VD+LG D+Y + ++ G+ + +++ Sbjct: 260 VSNFLYAWGPGGGFGGNRDVYLRTYPGDAFVDVLGLDTYDSTGSDAFLAGLVADLRMIAE 319 Query: 61 IADAKHKIAALTEFGYQ-NMKVAGNSTPDFYTRLASALKSNPDAKRMAFMLTWADFTPSE 119 IAD K K++A TEFG + G+S ++T++ +A+K++P A R A+M TWA+F + Sbjct: 320 IADEKGKVSAFTEFGVSGGVGTNGSSPAQWFTKVLAAIKADPVASRNAYMETWANFDAGQ 379 Query: 120 NSYVPYPLASGQEHQMMADFKSFFDDPYTLFSGEINGAYTQAVDTAGEQPFMHIASPTNQ 179 + +VP P ++ DF+++ DP+TLF+ E+ GA+ + V A QP +HIASP + Sbjct: 380 H-FVPVP-----GDALLEDFQAYAADPFTLFASEVTGAFDRTVAAAPAQPVVHIASPADG 433 Query: 180 AIVMEGTTLIRVRV 193 A V T +RVRV Sbjct: 434 ARVASAPTTVRVRV 447 Score = 95.5 bits (236), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 58/164 (35%), Positives = 86/164 (52%), Gaps = 7/164 (4%) Query: 454 VVDDFESYLGSDEALNTKFVHAGGDATETALDANKHGGSYGMKYTYTLGASGYAGVTKAL 513 VVDDFE Y G D AL ++V G + T + + GG+ ++ Y Y GV K L Sbjct: 526 VVDDFEGY-GDDTALRAEYVTYGAN-TISLETGSVGGGAKALRLDYDFATQTYTGVGKQL 583 Query: 514 NVDWSGFNALQFWYKPDAKGQKLVIQINAGGSTYEYYPDTATEQAALVTAKFSEFVPANG 573 + DWS FN L W PD ++V+Q+NAGG YE YP A ++ LVT F ++ PA Sbjct: 584 SGDWSDFNELAIWVDPDGSNNRMVLQLNAGGVAYEAYPSLAGDEPQLVTIPFVDWRPAPW 643 Query: 574 ATG----TLTKTNLKDVQKFSIYTNAKPDSMQLTSSMYFDDIKA 613 T ++ +L+ + F++Y N+ + + S+ DDI A Sbjct: 644 DTAHADRRMSDDDLRALTSFNVYVNSA-EGGAASGSLVVDDIAA 686 >C4IK30.1 C4IK30_CLOBU Beta-mannosidase Length = 1439 Score = 115 bits (289), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 81/209 (38%), Positives = 112/209 (53%), Gaps = 24/209 (11%) Query: 426 GLASGSIPVYIDNIGL-YSTYV----ETGSDPAVVDDFESYLGSDEALNTKFVHAGGDAT 480 G SG+I + DN L Y + V E P VVDDFESY+G L+ + G Sbjct: 924 GQVSGTIELKSDNTVLNYISAVFNIKEAEKQPRVVDDFESYMGDSSLLSGAWSTNKGPGC 983 Query: 481 ET---ALDANKHGGSYGMKYTYTLG----ASGYAGVTKALNVDWSGFNALQFWYKPDAKG 533 D NK+ G YG+ ++Y + + GYAG+ K+L DW+G +ALQ W KPD KG Sbjct: 984 SVNPKLSDKNKYTGDYGVAFSYKISTEKTSEGYAGIIKSLEADWTGCDALQIWAKPDGKG 1043 Query: 534 QKLVIQINAGGSTYE-YYPD-TATEQAALVTAKFSEFVPANGATGTLTKTNLKDVQKFSI 591 QKLVIQ+ + G +E P+ T T + L+ +FS+F+ G G L +N + +I Sbjct: 1044 QKLVIQLTSNGEDFEIRLPEFTKTTEPQLLKLQFSDFIGKKG--GKLDPSN---ITSMAI 1098 Query: 592 YTNA-KPDSMQ----LTSSMYFDDIKAVN 615 Y N P+ Q + S MYFDDIKA+N Sbjct: 1099 YCNTLVPEENQGSWTIDSIMYFDDIKAIN 1127 Score = 100 bits (249), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 57/214 (26%), Positives = 110/214 (51%), Gaps = 16/214 (7%) Query: 1 VHNFLYAYSPGGGFGTSGQQYLETYPGDDYVDILGFDSYYN-----GEGQSWFDGVATEA 55 +HNFLY YSP G F + ++YL YPGD+Y+DIL FD Y++ W + + Sbjct: 685 IHNFLYVYSPNGPF-ENDKEYLSRYPGDEYIDILAFDMYHDDPMSDASKDPWMESLKETI 743 Query: 56 ATLSKIADAKHKIAALTEFGYQ----NMKVAGNSTPDFYTRLASALKSNPDAKRMAFMLT 111 + IAD+K K++A++E G + M GN ++ ++ + + M + + Sbjct: 744 NLVQGIADSKGKLSAVSETGIRINGGGMPETGNPNKTWFKDVSDIISKSD----MPYYMV 799 Query: 112 WADFTPSENSYVPYPLASGQEHQMMADFKSFFDDPYTLFSGEINGAYTQAVDTAGEQPFM 171 WA+F ++N + P+ + H+M+ +F +++D T+F+ I+ + + + + Sbjct: 800 WANFNNTDNFFAPFMANDKKGHEMINEFIDYYNDKNTVFADRIDDYASVDTQISDKYAYG 859 Query: 172 HIASPTNQAIVMEGTTLIRVRVLNGSPAKVVYTV 205 +I +P + ++E T I+ V N + KV + + Sbjct: 860 YIMTPISNDRILEPVT-IKAAVRN-AEGKVTFDI 891 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,817,754,738 Number of extensions: 210645230 Number of successful extensions: 359138 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 359045 Number of HSP's successfully gapped: 53 Length of query: 783 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 639 Effective length of database: 1,704,070,043 Effective search space: 1088900757477 Effective search space used: 1088900757477 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 135 (56.6 bits)