BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0012.p (342 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7JA83.1 B7JA83_ACIF2 Homocitrate synthase EC=2.3.3.14 251 9e-65 B5ER60.1 B5ER60_ACIF5 Homocitrate synthase 251 9e-65 Q60C57.1 Q60C57_METCA Homocitrate synthase 250 1e-64 B1Y707.1 B1Y707_LEPCP Homocitrate synthase 243 3e-62 B2IEU7.1 B2IEU7_BEII9 Homocitrate synthase 241 7e-62 >B7JA83.1 B7JA83_ACIF2 Homocitrate synthase EC=2.3.3.14 Length = 386 Score = 251 bits (640), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 137/336 (40%), Positives = 191/336 (56%), Gaps = 2/336 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 + + E+GIPAMG+ E+E I IA++ LP +L+ W R D+ A+ V+ + S+PVS Sbjct: 47 IPELEIGIPAMGSEEEETIRGIAQMGLPARLVVWCRMHDTDLGAAKRCAVDMVNASVPVS 106 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 +IQ++GKLG L ++ + L GM V+VG ED+SRA + F++R++ R G Sbjct: 107 DIQIRGKLGKDRQWVLQQVDRRVKQVLDSGMDVAVGGEDASRAPLDFVLRVMEVAQRAGA 166 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 RFRYADT+ P A + L D+E+E+H HND GLA AN Sbjct: 167 RRFRYADTLGILEPFNTAHIMRRL--RAVTDMEIEIHAHNDLGLATANSVAALRFGATHV 224 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 +GER GNAA+EEVVM HHL+G ++ + K ++ V AS R V K IV Sbjct: 225 NTTVNGLGERAGNAALEEVVMCLHHLHGIETGIDVRQFKAISQLVALASARPVPAGKSIV 284 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G F+HESGIHVDGL+K YQ+FDP E+GR H VLGKHSGT + +LG IT Sbjct: 285 GDAIFSHESGIHVDGLLKNPRNYQSFDPEELGRQHHLVLGKHSGTKAIMRAYAELGSIIT 344 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQQ 336 A +L ++R Y HK T P + ++L+E + Sbjct: 345 ESQAQNILRQIRVYVLQHKVTPPVEDMHRFLLESME 380 >B5ER60.1 B5ER60_ACIF5 Homocitrate synthase Length = 386 Score = 251 bits (640), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 137/336 (40%), Positives = 191/336 (56%), Gaps = 2/336 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 + + E+GIPAMG+ E+E I IA++ LP +L+ W R D+ A+ V+ + S+PVS Sbjct: 47 IPELEIGIPAMGSEEEETIRGIAQMGLPARLVVWCRMHDTDLGAAKRCAVDMVNASVPVS 106 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 +IQ++GKLG L ++ + L GM V+VG ED+SRA + F++R++ R G Sbjct: 107 DIQIRGKLGKDRQWVLQQVDRRVKQVLDSGMDVAVGGEDASRAPLDFVLRVMEVAQRAGA 166 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 RFRYADT+ P A + L D+E+E+H HND GLA AN Sbjct: 167 RRFRYADTLGILEPFNTAHIMRRL--RAVTDMEIEIHAHNDLGLATANSVAALRFGATHV 224 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 +GER GNAA+EEVVM HHL+G ++ + K ++ V AS R V K IV Sbjct: 225 NTTVNGLGERAGNAALEEVVMCLHHLHGIETGIDVRQFKAISQLVALASARPVPAGKSIV 284 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G F+HESGIHVDGL+K YQ+FDP E+GR H VLGKHSGT + +LG IT Sbjct: 285 GDAIFSHESGIHVDGLLKNPRNYQSFDPEELGRQHHLVLGKHSGTKAIMRAYAELGSIIT 344 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQQ 336 A +L ++R Y HK T P + ++L+E + Sbjct: 345 ESQAQNILRQIRVYVLQHKVTPPVEDMHRFLLESME 380 >Q60C57.1 Q60C57_METCA Homocitrate synthase Length = 384 Score = 250 bits (639), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 144/339 (42%), Positives = 188/339 (55%), Gaps = 2/339 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 V + EVGIP MG EQE I AIA L L +LM W R D+ A G + ++SIPVS Sbjct: 41 VPEMEVGIPVMGAEEQEVIRAIAGLGLSARLMVWGRMCERDLAAAADCGADRVNLSIPVS 100 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 +I L+ K+ + + L + + L G+ V VG ED+SRAD F++++ + R G Sbjct: 101 DIHLRHKVNRTREWVLASIDRFVKRGLDAGLEVCVGCEDASRADPDFMLQVAEAAQRAGA 160 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R R+ADT+ P ER+ L V D+E+E+H HNDFGLA AN Sbjct: 161 RRIRFADTLGLLEPMSTFERIRRL--AVATDLEIEMHAHNDFGLATANTLMAVQAGASHV 218 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 +GER GNAA+EE VMA H LYG D+ + D ++ V AASGR V K IV Sbjct: 219 NTTVNGLGERAGNAAIEETVMALHQLYGIDTGIAVDHFPEISGLVAAASGRPVAANKSIV 278 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G+ FTHE+GIHVDGL+K YQ DP ++GR HR VLGKHSGT V QLG+ ++ Sbjct: 279 GEAVFTHEAGIHVDGLLKNLINYQGVDPKDLGREHRLVLGKHSGTHAVKRSFRQLGIELS 338 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQQRAQ 339 AGRLL R+R +A K E L + E +Q Sbjct: 339 ETDAGRLLKRIRIHASQTKRPPSETELKLFYAETSVSSQ 377 >B1Y707.1 B1Y707_LEPCP Homocitrate synthase Length = 384 Score = 243 bits (619), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 140/320 (43%), Positives = 184/320 (57%), Gaps = 3/320 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 V + EVG AMG E + I AI + L + M W R D+ A G + H+++PVS Sbjct: 44 VPEMEVGAAAMGEAEIDLIRAITQSGLKARTMVWARMSELDLRSAARCGADLVHLAVPVS 103 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 ++Q++ KLG L + A G+T SVG+ED+SRAD GFL R+ ++ G Sbjct: 104 DLQIERKLGRDRGWVLGMVQRCVSAARDAGLTPSVGLEDASRADRGFLARVADTAQAAGA 163 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R RYADT+ P +R+ L D+E+E+H HND GLA AN Sbjct: 164 VRVRYADTLGILDPFGTFDRIGELRRST--DLEIEIHAHNDLGLATANTLAAFRAGATHA 221 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 +GER GNAA+EE+VMA HL+G +S V L L+ V A+GR V K IV Sbjct: 222 STTVNGLGERAGNAALEEIVMALRHLHGVESGVDSRALPHLSALVAQAAGRPVAAGKCIV 281 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G F HESGIHVDGL+K+R Y++FDP+E+GRSHRFVLGKHSG+ GV E+LGL + Sbjct: 282 GDAVFWHESGIHVDGLLKDRRNYESFDPAELGRSHRFVLGKHSGSAGVRAACERLGLPMP 341 Query: 301 PDTAG-RLLVRVREYAESHK 319 DTA RLL R+R +A K Sbjct: 342 DDTAAPRLLARIRAHAVDTK 361 >B2IEU7.1 B2IEU7_BEII9 Homocitrate synthase Length = 400 Score = 241 bits (615), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 135/335 (40%), Positives = 188/335 (56%), Gaps = 2/335 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 V + E G PAMG RE I A+ LP K++ W R +P DID A A+GV ++S+PVS Sbjct: 52 VPEIEAGTPAMGEREIAAINAVVAEGLPSKIIAWCRMVPQDIDAAVASGVGMINLSLPVS 111 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 +IQL+ K G + YA G+ V++G ED+SRAD+ FL+ + G+ Sbjct: 112 DIQLKAKFGAGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLLEVAELGASLGV 171 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R R+ADT+ P + L D+E+E+H H+D GLA AN Sbjct: 172 RRMRFADTLGVLDPFMTFAMIERL--RQASDLEIEIHAHDDLGLATANTLAALRAGATHA 229 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 +GER GNA +EEV +A LYG + V L +A V A+GRS+ +AK IV Sbjct: 230 SVTVVGLGERAGNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIV 289 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G+ FTHESGIHVDGL+K+ YQ DP+ +GRSH VLGKHSG V + L + GL+++ Sbjct: 290 GEAVFTHESGIHVDGLLKDLHCYQALDPALLGRSHHVVLGKHSGLRAVTNALAEQGLSVS 349 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQ 335 D A +L R++ YAE K +VP +L+ + E + Sbjct: 350 ADEAKFVLARIKLYAEQEKASVPGDILLDFYREAK 384 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,960,846,055 Number of extensions: 78731711 Number of successful extensions: 180746 Number of sequences better than 1.0e-05: 2104 Number of HSP's gapped: 175283 Number of HSP's successfully gapped: 2104 Length of query: 342 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 206 Effective length of database: 1,779,438,163 Effective search space: 366564261578 Effective search space used: 366564261578 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)