BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0151.0009.p
         (136 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Q56JU0.1 Q56JU0_9BACL NifX (Fragment)                                 153   5e-36
Q2J4G3.1 Q2J4G3_FRASC Dinitrogenase iron-molybdenum cofactor bio...   108   1e-22
A8L2B9.1 A8L2B9_FRASN Nitrogen fixation protein NifX                  107   4e-22
Q60C75.1 Q60C75_METCA Nitrogenase molybdenum-iron protein NifX        100   4e-20
B5YH14.1 B5YH14_THEYD Nitrogen fixation protein NifX                  100   5e-20

>Q56JU0.1 Q56JU0_9BACL NifX (Fragment)
          Length = 120

 Score =  153 bits (387), Expect = 5e-36,   Method: Compositional matrix adjust.
 Identities = 73/120 (60%), Positives = 92/120 (76%)

Query: 1   VKVAFATDDGSRVNAHFGQSPMFAVYNVTKNGGELVELRKLPAVLNQDEAGKIDCRLTAV 60
           +KVAF TDDG  VNAHFGQSPMFA+Y VT  G EL+E R++       EAGKI  R+  +
Sbjct: 1   MKVAFTTDDGILVNAHFGQSPMFAIYEVTPTGIELLEHRQVLPESELHEAGKIASRIRLI 60

Query: 61  EDCTLIFIMQIGASAAARVTRRRIMPVKVPFGSPIDEQVKRLVEMLQGKPPMWLAKVLRA 120
           EDC L+F++QIGA+AAA+VT+R IMPVKV   SPI++Q++RL +ML  KPPMWLAK+L A
Sbjct: 61  EDCVLVFLVQIGAAAAAQVTKRTIMPVKVAINSPIEDQLQRLRQMLLRKPPMWLAKILHA 120


>Q2J4G3.1 Q2J4G3_FRASC Dinitrogenase iron-molybdenum cofactor biosynthesis
          Length = 144

 Score =  108 bits (271), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 57/122 (46%), Positives = 73/122 (59%), Gaps = 1/122 (0%)

Query: 1   VKVAFATDDGSRVNAHFGQSPMFAVYNVTKNGGELVELRKLPAVLNQDEAGKIDCRLTAV 60
           +K+AFAT DG+ V+ HFG    F VY V   G   +E R L A    DE  KI+ RL AV
Sbjct: 2   LKIAFATSDGTAVDQHFGWCQRFDVYEVGPEGARFLETRALDAAPT-DEQDKIESRLAAV 60

Query: 61  EDCTLIFIMQIGASAAARVTRRRIMPVKVPFGSPIDEQVKRLVEMLQGKPPMWLAKVLRA 120
            DC ++ I  IG +AAA+V + R+ PVKV  G+ I E ++R    L G PP WL KVLR+
Sbjct: 61  SDCAILNISDIGGTAAAKVVKARVHPVKVVKGTAIPELLRRYTSTLAGNPPPWLRKVLRS 120

Query: 121 EE 122
            E
Sbjct: 121 HE 122


>A8L2B9.1 A8L2B9_FRASN Nitrogen fixation protein NifX
          Length = 148

 Score =  107 bits (267), Expect = 4e-22,   Method: Compositional matrix adjust.
 Identities = 59/123 (47%), Positives = 74/123 (60%), Gaps = 3/123 (2%)

Query: 1   VKVAFATDDGSRVNAHFGQSPMFAVYNVTKNGGELVELRKL-PAVLNQDEAGKIDCRLTA 59
           +K+AFAT DG  V+ HFG    F VY V   G  L E R+L PA    DE  KI  RL A
Sbjct: 2   LKIAFATSDGHAVDQHFGWCRRFDVYEVGPEGFHLFETRELGPA--EDDEEDKIASRLAA 59

Query: 60  VEDCTLIFIMQIGASAAARVTRRRIMPVKVPFGSPIDEQVKRLVEMLQGKPPMWLAKVLR 119
           V DC ++ +  IG +AAA+V + RI P+KVP G+ ID  + + V +L G PP WL KVLR
Sbjct: 60  VVDCAILNVCDIGGTAAAKVVKARIHPMKVPVGTEIDALLAKFVGVLAGNPPPWLRKVLR 119

Query: 120 AEE 122
           A  
Sbjct: 120 AHS 122


>Q60C75.1 Q60C75_METCA Nitrogenase molybdenum-iron protein NifX
          Length = 141

 Score =  100 bits (249), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 52/122 (42%), Positives = 75/122 (61%), Gaps = 1/122 (0%)

Query: 1   VKVAFATDDGSRVNAHFGQSPMFAVYNVTKNGGELVELRKLPAVLNQD-EAGKIDCRLTA 59
           +KVAFAT D  RV+AHFG +   AVY ++  G + VE  +    L +D    K+  +L A
Sbjct: 1   MKVAFATQDLKRVDAHFGWAKNIAVYELSAEGADFVEAIQFEGDLQEDGNEDKLAPKLEA 60

Query: 60  VEDCTLIFIMQIGASAAARVTRRRIMPVKVPFGSPIDEQVKRLVEMLQGKPPMWLAKVLR 119
           ++DC ++++  IG S AARV  + I P+KVP   PI E + +L ++L+G PP WL KVL 
Sbjct: 61  IKDCAILYVAAIGGSGAARVVAQGIHPIKVPQPEPIAELLDKLRDVLRGNPPPWLRKVLA 120

Query: 120 AE 121
            E
Sbjct: 121 KE 122


>B5YH14.1 B5YH14_THEYD Nitrogen fixation protein NifX
          Length = 133

 Score =  100 bits (248), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 51/133 (38%), Positives = 83/133 (62%), Gaps = 10/133 (7%)

Query: 1   VKVAFATDDGSRVNAHFGQSPMFAVYNVTKNGGELVELRKLP-----AVLNQDEAGKIDC 55
           +KVAFA+ DG RVN HFG+S  F VY V + G + +E+RK       A+    +  ++  
Sbjct: 1   MKVAFASTDGIRVNEHFGRSGQFVVYEVEEEGYKFMEIRKFSEGRDRAIEESKDTPEVHN 60

Query: 56  -----RLTAVEDCTLIFIMQIGASAAARVTRRRIMPVKVPFGSPIDEQVKRLVEMLQGKP 110
                ++ A+ DC +I++ +IG  +AAR++++ IMP+KV  GS I+E +++L E ++  P
Sbjct: 61  DLVWDKVKALSDCKIIYMTEIGPPSAARLSQKGIMPLKVKEGSSIEECLEKLYETIKTSP 120

Query: 111 PMWLAKVLRAEEE 123
           P WL K+L  +EE
Sbjct: 121 PPWLKKLLNKKEE 133


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.319    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 772,285,577
Number of extensions: 27332587
Number of successful extensions: 59890
Number of sequences better than 1.0e-05: 141
Number of HSP's gapped: 59837
Number of HSP's successfully gapped: 141
Length of query: 136
Length of database: 3,060,696,203
Length adjustment: 100
Effective length of query: 36
Effective length of database: 2,118,594,703
Effective search space: 76269409308
Effective search space used: 76269409308
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 126 (53.1 bits)