BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0004.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9X242.1 B9X242_9BACL Nitrogenase iron protein EC=1.18.6.1 566 e-160 Q5EC35.1 Q5EC35_9BACL Nitrogenase iron protein EC=1.18.6.1 509 e-142 Q7WTA8.1 Q7WTA8_PAEAZ Nitrogenase iron protein EC=1.18.6.1 494 e-138 B9X2A0.1 B9X2A0_9BACL Nitrogenase iron protein EC=1.18.6.1 492 e-137 Q9AKT8.1 NIFH1_PAEAZ Nitrogenase iron protein 1 EC=1.18.6.1 486 e-135 >B9X242.1 B9X242_9BACL Nitrogenase iron protein EC=1.18.6.1 Length = 289 Score = 566 bits (1459), Expect = e-160, Method: Compositional matrix adjust. Identities = 277/289 (95%), Positives = 279/289 (96%) Query: 1 MMRQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLE 60 MMRQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLE Sbjct: 1 MMRQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLE 60 Query: 61 LAAELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLD 120 LAAELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLD Sbjct: 61 LAAELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLD 120 Query: 121 FVSYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLG 180 FVSYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLG Sbjct: 121 FVSYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLG 180 Query: 181 GLICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEY 240 GLICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEY Sbjct: 181 GLICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEY 240 Query: 241 EILAEKILNNKMLTIPTPISXXXXXXXXXXFGIIEDEEAALKKLQASGQ 289 EILAEKILNNKMLTIPTPIS FGIIEDEEAA+KKL+ASGQ Sbjct: 241 EILAEKILNNKMLTIPTPISMEELEELLMEFGIIEDEEAAIKKLKASGQ 289 >Q5EC35.1 Q5EC35_9BACL Nitrogenase iron protein EC=1.18.6.1 Length = 288 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 246/288 (85%), Positives = 259/288 (89%) Query: 2 MRQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLEL 61 MRQIAFYGKGGIGKSTTSQNTLAQLATKF Q+IMIVGCDPKADSTRLILNTKAQ +VL L Sbjct: 1 MRQIAFYGKGGIGKSTTSQNTLAQLATKFNQKIMIVGCDPKADSTRLILNTKAQQTVLHL 60 Query: 62 AAELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLDF 121 AAE G+VEDLEL DV+Q GFG I+NVECGGPEPGVGCAGRGIITAINFLE+EGAY+ LDF Sbjct: 61 AAERGTVEDLELTDVVQQGFGSILNVECGGPEPGVGCAGRGIITAINFLEEEGAYEGLDF 120 Query: 122 VSYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLGG 181 VSYDVLGDVVCGGFAMPIRE KAQEIYIVCSGEMMAMYAANNIARGILKYA SGGVRLGG Sbjct: 121 VSYDVLGDVVCGGFAMPIREKKAQEIYIVCSGEMMAMYAANNIARGILKYANSGGVRLGG 180 Query: 182 LICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEYE 241 LICNSRNTDRE ELIMELARRLNTQMIHF+PRDN+VQHAELRRMTV QYNP H+QA EYE Sbjct: 181 LICNSRNTDREAELIMELARRLNTQMIHFLPRDNVVQHAELRRMTVTQYNPEHKQAAEYE 240 Query: 242 ILAEKILNNKMLTIPTPISXXXXXXXXXXFGIIEDEEAALKKLQASGQ 289 LA KILNN MLT+PTPI+ FGIIEDEE AL KL+ASGQ Sbjct: 241 ALAGKILNNDMLTVPTPITMEELEELLMEFGIIEDEETALNKLEASGQ 288 >Q7WTA8.1 Q7WTA8_PAEAZ Nitrogenase iron protein EC=1.18.6.1 Length = 292 Score = 494 bits (1273), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/287 (82%), Positives = 255/287 (88%) Query: 3 RQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLELA 62 RQIAFYGKGGIGKSTTSQNTLAQLAT F Q+IMIVGCDPKADSTRLILNTKAQ +VL +A Sbjct: 6 RQIAFYGKGGIGKSTTSQNTLAQLATTFGQKIMIVGCDPKADSTRLILNTKAQQTVLHMA 65 Query: 63 AELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLDFV 122 AELGS EDLELEDVL TGFGDI+ VE GGPEPGVGCAGRGIIT+INFLE++GAY +DF+ Sbjct: 66 AELGSAEDLELEDVLATGFGDILCVESGGPEPGVGCAGRGIITSINFLEEQGAYDGMDFI 125 Query: 123 SYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLGGL 182 SYDVLGDVVCGGFAMPIRE KAQEIYIVCSGEMMAMYAANNIARGILKYA SG VRLGGL Sbjct: 126 SYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNIARGILKYAQSGSVRLGGL 185 Query: 183 ICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEYEI 242 ICNSRNTDREDELIMELARRLNTQMIHFVPRDN+VQHAELRRMTV QYNP H QA EY+ Sbjct: 186 ICNSRNTDREDELIMELARRLNTQMIHFVPRDNVVQHAELRRMTVTQYNPEHNQANEYKQ 245 Query: 243 LAEKILNNKMLTIPTPISXXXXXXXXXXFGIIEDEEAALKKLQASGQ 289 LA+KIL+N+ML IPTPI FG++EDEE ALKKL+A+G Sbjct: 246 LADKILHNEMLNIPTPIEMDELEQLLIDFGVVEDEETALKKLEAAGH 292 >B9X2A0.1 B9X2A0_9BACL Nitrogenase iron protein EC=1.18.6.1 Length = 292 Score = 492 bits (1266), Expect = e-137, Method: Compositional matrix adjust. Identities = 236/287 (82%), Positives = 255/287 (88%) Query: 3 RQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLELA 62 RQIAFYGKGGIGKSTTSQNTLAQLAT F Q+IMIVGCDPKADSTRLILNTKAQ +VL +A Sbjct: 6 RQIAFYGKGGIGKSTTSQNTLAQLATTFGQKIMIVGCDPKADSTRLILNTKAQQTVLHMA 65 Query: 63 AELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLDFV 122 AELGSVEDLELEDV+ TGFGDI+ VE GGPEPGVGCAGRGIIT+INFLE+ GAY +DF+ Sbjct: 66 AELGSVEDLELEDVVATGFGDILCVESGGPEPGVGCAGRGIITSINFLEELGAYDGMDFI 125 Query: 123 SYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLGGL 182 SYDVLGDVVCGGFAMPIRE KAQEIYIVCSGEMMAMYAANNIARGILKYA SG VRLGGL Sbjct: 126 SYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNIARGILKYAQSGSVRLGGL 185 Query: 183 ICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEYEI 242 ICNSRNTDREDELIMELARRLNTQMIHFVPRDN+VQHAELRRMTV QYNP H QA EY+ Sbjct: 186 ICNSRNTDREDELIMELARRLNTQMIHFVPRDNVVQHAELRRMTVTQYNPEHNQANEYKQ 245 Query: 243 LAEKILNNKMLTIPTPISXXXXXXXXXXFGIIEDEEAALKKLQASGQ 289 LA+KIL+N+ML IPTPI FG++EDEE A+KKL+A+G Sbjct: 246 LADKILHNEMLNIPTPIEMDELEQLLIDFGVVEDEETAIKKLEAAGH 292 >Q9AKT8.1 NIFH1_PAEAZ Nitrogenase iron protein 1 EC=1.18.6.1 Length = 292 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 234/287 (81%), Positives = 253/287 (88%) Query: 3 RQIAFYGKGGIGKSTTSQNTLAQLATKFKQRIMIVGCDPKADSTRLILNTKAQNSVLELA 62 RQIAFYGKGGIGKSTTSQNTLAQLAT F Q+IMIVGCDPKADSTRLILNTKAQ +VL LA Sbjct: 6 RQIAFYGKGGIGKSTTSQNTLAQLATTFGQKIMIVGCDPKADSTRLILNTKAQQTVLHLA 65 Query: 63 AELGSVEDLELEDVLQTGFGDIINVECGGPEPGVGCAGRGIITAINFLEQEGAYQDLDFV 122 AELGSVEDLELEDV+ TGFG I+ VE GGPEPGVGCAGRGIIT+INFLE++GAY +DF+ Sbjct: 66 AELGSVEDLELEDVVATGFGGILCVESGGPEPGVGCAGRGIITSINFLEEQGAYDGMDFI 125 Query: 123 SYDVLGDVVCGGFAMPIREGKAQEIYIVCSGEMMAMYAANNIARGILKYATSGGVRLGGL 182 SYDVLGDVVCGGFAMPIRE KAQEIYIVCSGEMMAMYAANNIARGILKYA SG VRLGGL Sbjct: 126 SYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNIARGILKYAQSGSVRLGGL 185 Query: 183 ICNSRNTDREDELIMELARRLNTQMIHFVPRDNIVQHAELRRMTVAQYNPAHQQAKEYEI 242 ICNSR TDREDELI ELARRLNTQMIHFVPRDN+VQHAELRRMTV QYNP H QA EY+ Sbjct: 186 ICNSRKTDREDELITELARRLNTQMIHFVPRDNVVQHAELRRMTVTQYNPEHPQANEYKQ 245 Query: 243 LAEKILNNKMLTIPTPISXXXXXXXXXXFGIIEDEEAALKKLQASGQ 289 LA+KIL+N+ LTIPTPI FG++EDEE A+KKL+A+G Sbjct: 246 LADKILHNEKLTIPTPIEMDELEQLLIDFGVVEDEETAIKKLEAAGH 292 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,575,005,985 Number of extensions: 62793543 Number of successful extensions: 199320 Number of sequences better than 1.0e-05: 10931 Number of HSP's gapped: 188436 Number of HSP's successfully gapped: 10931 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)