BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0003.p (456 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9X2B9.1 B9X2B9_9BACL NifB protein 799 0.0 B9X299.1 B9X299_9BACL NifB protein 775 0.0 Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein 773 0.0 B9X241.1 B9X241_9BACL NifB protein (Fragment) 593 e-167 Q56JU5.1 Q56JU5_9BACL NifB 558 e-157 >B9X2B9.1 B9X2B9_9BACL NifB protein Length = 458 Score = 799 bits (2064), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/458 (83%), Positives = 421/458 (91%), Gaps = 2/458 (0%) Query: 1 MMLPSPCISSEAEEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRP 60 MM P C SEAEEEISRHPCYSEEAHRF+ARMHIPVAPACNIQC+YCNRKFDCVNESRP Sbjct: 1 MMQPLSCTPSEAEEEISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRP 60 Query: 61 GVVSEVLTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSL 120 GVVSEVLTP+QAERKVKGVAAQLMQLSVVGIAGPGDPLANPE TFDTFARVK++VPDVSL Sbjct: 61 GVVSEVLTPQQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEATFDTFARVKEHVPDVSL 120 Query: 121 CLSTNGLTLYRHIDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLI 180 CLSTNGLTLYR++DEIVELGIRHVTITINAID DVG+ IYPW DEG+RYEG+ AAELLI Sbjct: 121 CLSTNGLTLYRYVDEIVELGIRHVTITINAIDPDVGREIYPWAVDEGIRYEGREAAELLI 180 Query: 181 SRQLRGLELLAKLGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQY 240 +RQL G+E+LAK GILVKVNSIMIPGVNDHHL AVSK+VKELGATLHNVTPLIIAPGSQY Sbjct: 181 TRQLLGVEMLAKRGILVKVNSIMIPGVNDHHLVAVSKKVKELGATLHNVTPLIIAPGSQY 240 Query: 241 EKDGRKAPRPKELHNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPV 300 EKDGRKAPRPKELH +QE LG+ GMKVMRHCRQCRADAIGLLGQDRN+DFPLEAME +P+ Sbjct: 241 EKDGRKAPRPKELHTVQEQLGRDGMKVMRHCRQCRADAIGLLGQDRNQDFPLEAMEENPL 300 Query: 301 INAEARALFQGELDVKIRERVQAKEARL--LRENAPKIRVAVATRGGDKVNQHFGHATEF 358 IN +AR FQ +LD+KIRER AK+AR +R+ A R+AVATRGGDKVNQHFGHATEF Sbjct: 301 INMDARMQFQSDLDIKIRERNAAKQARREGVRQGAVASRIAVATRGGDKVNQHFGHATEF 360 Query: 359 LVYDTDGAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRA 418 ++YDTDG +++L+GVRKIQAYCHGTADCNGDKAATLQEI+SI+SDCRILLCSGIG+ P+ Sbjct: 361 MIYDTDGTDIKLIGVRKIQAYCHGTADCNGDKAATLQEILSILSDCRILLCSGIGEGPKI 420 Query: 419 SLNKAGVLPLVRKGGIQEAILESVKYSSYFENINISKG 456 LNK G+LPLVRKG I+EAILESVKYSSYFEN+N+SKG Sbjct: 421 HLNKRGILPLVRKGDIREAILESVKYSSYFENMNMSKG 458 >B9X299.1 B9X299_9BACL NifB protein Length = 458 Score = 775 bits (2002), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/455 (82%), Positives = 410/455 (90%), Gaps = 3/455 (0%) Query: 4 PSPCISSEAEEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVV 63 P+ C SS AE++ISRHPCYSEEAHRF+ARMHIPVAPACNIQC+YCNRKFDCVNESRPGVV Sbjct: 5 PTSCSSSAAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVV 64 Query: 64 SEVLTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLS 123 SEVLTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLAN ++TFDTFARVKK+VPDV CLS Sbjct: 65 SEVLTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVMTCLS 124 Query: 124 TNGLTLYRHIDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQ 183 TNGLTLYRHID IVELGI HVTITINAID DVG+ IYPWVFDEGVRYEG+ AA LLISRQ Sbjct: 125 TNGLTLYRHIDRIVELGIGHVTITINAIDPDVGKEIYPWVFDEGVRYEGREAAALLISRQ 184 Query: 184 LRGLELLAKLGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQYEKD 243 L+ +E LAK GILVKVNSIMIPGVND HL VSK+VKELGATLHNVTPLIIAPGSQYEKD Sbjct: 185 LQEVEELAKRGILVKVNSIMIPGVNDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKD 244 Query: 244 GRKAPRPKELHNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPVINA 303 GRKAPRPKEL+ LQE L +GG K+MRHCRQCRADA+GLLGQDRN+DF LE MEADPVI+ Sbjct: 245 GRKAPRPKELNQLQEQLSEGGTKLMRHCRQCRADAVGLLGQDRNQDFQLETMEADPVIDL 304 Query: 304 EARALFQGELDVKIRERVQAKEAR--LLRENAPKIRVAVATRGGDKVNQHFGHATEFLVY 361 +AR FQ ELD KIR+R+ AKE R +L E+ K RVAVA+RGGDKVNQHFGHATEF+++ Sbjct: 305 DAREQFQKELDGKIRQRLIAKENRRTILDEDK-KTRVAVASRGGDKVNQHFGHATEFMIF 363 Query: 362 DTDGAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLN 421 DTDG EV+ +G+RKIQAYCHGTADCNGDK TLQEI SI+SDCRILL SGIGD+PRA LN Sbjct: 364 DTDGTEVKFIGIRKIQAYCHGTADCNGDKDETLQEITSILSDCRILLSSGIGDAPRAVLN 423 Query: 422 KAGVLPLVRKGGIQEAILESVKYSSYFENINISKG 456 +AGVLPLVRKGGIQEAILESVKY SYFENINISKG Sbjct: 424 RAGVLPLVRKGGIQEAILESVKYGSYFENINISKG 458 >Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein Length = 458 Score = 773 bits (1995), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/452 (82%), Positives = 409/452 (90%), Gaps = 3/452 (0%) Query: 7 CISSEAEEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVSEV 66 C +S AE++ISRHPCYSEEAHRF+ARMHIPVAPACNIQC+YCNRKFDCVNESRPGVVSEV Sbjct: 8 CSTSAAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEV 67 Query: 67 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLSTNG 126 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLAN ++TFDTFARVKK+VPDV CLSTNG Sbjct: 68 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVITCLSTNG 127 Query: 127 LTLYRHIDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRG 186 LTLYRHID IVELGI HVTITINAID DVG+ IYPWV DEGVRYEG+ AA LLISRQL+G Sbjct: 128 LTLYRHIDRIVELGIGHVTITINAIDPDVGKEIYPWVSDEGVRYEGREAAALLISRQLQG 187 Query: 187 LELLAKLGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQYEKDGRK 246 +E LAK GILVKVNSI+IPG+ND HL VSK+VKELGATLHNVTPLIIAPGSQYEKDGRK Sbjct: 188 VEELAKRGILVKVNSILIPGINDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRK 247 Query: 247 APRPKELHNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPVINAEAR 306 APRP+EL+ LQE L +GG K+MRHCRQCRADA+GLLGQDRN+DF LE MEADPVI+ +AR Sbjct: 248 APRPRELNQLQEQLSEGGTKLMRHCRQCRADAVGLLGQDRNQDFQLETMEADPVIDLDAR 307 Query: 307 ALFQGELDVKIRERVQAKEAR--LLRENAPKIRVAVATRGGDKVNQHFGHATEFLVYDTD 364 FQ ELD KIR+R+ AKE R +L EN K RVAVA+RGGDKVNQHFGHATEF+++DTD Sbjct: 308 EQFQQELDSKIRQRLIAKENRRSILDENK-KTRVAVASRGGDKVNQHFGHATEFMIFDTD 366 Query: 365 GAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLNKAG 424 GAEV+ +G+RKIQAYCHGTADCNGDK TLQEI SI+SDCRILL SGIGD+PRA+LNKAG Sbjct: 367 GAEVKFIGIRKIQAYCHGTADCNGDKGETLQEITSILSDCRILLSSGIGDAPRAALNKAG 426 Query: 425 VLPLVRKGGIQEAILESVKYSSYFENINISKG 456 VLPLVRKGGIQEAILESVKY SYFENI ISKG Sbjct: 427 VLPLVRKGGIQEAILESVKYGSYFENITISKG 458 >B9X241.1 B9X241_9BACL NifB protein (Fragment) Length = 304 Score = 593 bits (1529), Expect = e-167, Method: Compositional matrix adjust. Identities = 290/304 (95%), Positives = 296/304 (97%) Query: 153 ADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRGLELLAKLGILVKVNSIMIPGVNDHHL 212 ADVGQAIYPWVFDEGVRYEGKAAAELLISRQL GLELLAKLGILVKVNSIMIPGVNDHHL Sbjct: 1 ADVGQAIYPWVFDEGVRYEGKAAAELLISRQLTGLELLAKLGILVKVNSIMIPGVNDHHL 60 Query: 213 PAVSKRVKELGATLHNVTPLIIAPGSQYEKDGRKAPRPKELHNLQELLGQGGMKVMRHCR 272 PAVSKRVKELGATLHNVTPLIIAPGSQYE+DGRKAPRPKELHNLQELLG+GGMKVMRHCR Sbjct: 61 PAVSKRVKELGATLHNVTPLIIAPGSQYEQDGRKAPRPKELHNLQELLGRGGMKVMRHCR 120 Query: 273 QCRADAIGLLGQDRNRDFPLEAMEADPVINAEARALFQGELDVKIRERVQAKEARLLREN 332 QCRADAIGLLGQDRN+DFPLEAMEADP INAEARALFQGELDVKIRERVQAK ARLL+EN Sbjct: 121 QCRADAIGLLGQDRNQDFPLEAMEADPAINAEARALFQGELDVKIRERVQAKRARLLQEN 180 Query: 333 APKIRVAVATRGGDKVNQHFGHATEFLVYDTDGAEVQLLGVRKIQAYCHGTADCNGDKAA 392 APKIRVAVATRGGDKVNQHFGHATEFLVYDTDGA VQLLGVRKIQAYCHG ADCNGDKAA Sbjct: 181 APKIRVAVATRGGDKVNQHFGHATEFLVYDTDGAVVQLLGVRKIQAYCHGKADCNGDKAA 240 Query: 393 TLQEIISIVSDCRILLCSGIGDSPRASLNKAGVLPLVRKGGIQEAILESVKYSSYFENIN 452 TLQEIISI+SDCRILLCSGIG+ PR SLNK GVLPLVRKGGIQEAILESVKYSSYFENIN Sbjct: 241 TLQEIISILSDCRILLCSGIGEGPRTSLNKIGVLPLVRKGGIQEAILESVKYSSYFENIN 300 Query: 453 ISKG 456 ISKG Sbjct: 301 ISKG 304 >Q56JU5.1 Q56JU5_9BACL NifB Length = 473 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 268/439 (61%), Positives = 331/439 (75%), Gaps = 21/439 (4%) Query: 14 EEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVSEVLTPEQAE 73 E + RHPCY+E+AHR++ARM +PVAPACNIQCHYCNRKFDCVNESRPGVVSE+LTPE A Sbjct: 25 EMLMRHPCYNEDAHRYFARMRLPVAPACNIQCHYCNRKFDCVNESRPGVVSELLTPELAA 84 Query: 74 RKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLSTNGLTLYRHI 133 RK GVAAQLMQLSVVGIAGPGDPLANP+ TF+TF +V+ +V DV CLSTNGLTL RH+ Sbjct: 85 RKTIGVAAQLMQLSVVGIAGPGDPLANPKATFETFRQVRDSVKDVIFCLSTNGLTLIRHV 144 Query: 134 DEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRGLELLAKL 193 +EIV LGI HVTITINA+D +G IY WV+D GVRYEG+ A LLI RQL GL +L+ Sbjct: 145 EEIVNLGISHVTITINAVDPCIGSRIYGWVYDGGVRYEGEEGAALLIQRQLEGLSMLSSR 204 Query: 194 GILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQYEKDGRKAPRPKEL 253 G+LVKVNS++IP VND HLP V++ VK GA +HN+ PLIIAPGS+YE++GR+APRP+ + Sbjct: 205 GVLVKVNSVLIPDVNDAHLPEVARVVKGHGAAIHNIMPLIIAPGSRYEQEGRQAPRPQVV 264 Query: 254 HNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPVINAEARALFQGEL 313 +QE G +MRHCRQCRADAIGLLG+DRN DF E +E P ++ +ARA EL Sbjct: 265 RQVQEQCAGAGAVIMRHCRQCRADAIGLLGEDRNADFTWEKLEDAPALDDQARARLHREL 324 Query: 314 DVKIRERVQAKEARLLRENAPK---------------------IRVAVATRGGDKVNQHF 352 D ++R R++ KE R + K + VAVA+RG KVNQHF Sbjct: 325 DERVRVRMERKEERDKGKATRKGVGCSCPSNGSGSGAEFASSPVLVAVASRGHGKVNQHF 384 Query: 353 GHATEFLVYDTDGAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGI 412 G A EF++Y++DG VQ +G+RK+Q+YCHGTADCNGDKA T++EII IV DC +LL SGI Sbjct: 385 GRAKEFMIYESDGTVVQFVGIRKVQSYCHGTADCNGDKAETMKEIIQIVQDCALLLSSGI 444 Query: 413 GDSPRASLNKAGVLPLVRK 431 GD+P+ L + GVLP+VR+ Sbjct: 445 GDAPKEVLQEHGVLPIVRR 463 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,670,582,966 Number of extensions: 110809488 Number of successful extensions: 268588 Number of sequences better than 1.0e-05: 321 Number of HSP's gapped: 268351 Number of HSP's successfully gapped: 326 Length of query: 456 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 317 Effective length of database: 1,751,175,118 Effective search space: 555122512406 Effective search space used: 555122512406 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)