BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0038.p (315 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2BCX6.1 B2BCX6_PAEPO NAD binding D-isomer specific 2-hydroxyaci... 568 e-160 A6LZ51.1 A6LZ51_CLOB8 D-isomer specific 2-hydroxyacid dehydrogen... 504 e-141 C2BXQ9.1 C2BXQ9_LISGR NAD binding D-isomer specific 2-hydroxyaci... 386 e-105 C2ESK5.1 C2ESK5_9LACO NAD binding D-isomer specific 2-hydroxyaci... 282 3e-74 O29445.1 SERA_ARCFU D-3-phosphoglycerate dehydrogenase EC=1.1.1.95 158 8e-37 >B2BCX6.1 B2BCX6_PAEPO NAD binding D-isomer specific 2-hydroxyacid dehydrogenase Length = 321 Score = 568 bits (1465), Expect = e-160, Method: Compositional matrix adjust. Identities = 272/315 (86%), Positives = 283/315 (89%) Query: 1 MKILIVGYFNETSKSNIARYFPQDWNVVVVPPGQEMLHHIEDCQVIIPEHIKVDHSLLSI 60 MKILIVGYF+ETSKSNI RYFPQDWNVV+VPPG+EMLHHIEDCQVIIPEHIKVDHSLLSI Sbjct: 7 MKILIVGYFSETSKSNIVRYFPQDWNVVIVPPGKEMLHHIEDCQVIIPEHIKVDHSLLSI 66 Query: 61 AKKLKLVQTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSYYKNIPFLDTF 120 AKKLKLVQTGAGFDNVDVPACTQLGIW EHVMALILSYYKNIPFLDTF Sbjct: 67 AKKLKLVQTGAGFDNVDVPACTQLGIWVANAAGVNAQAVAEHVMALILSYYKNIPFLDTF 126 Query: 121 MKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAYARNAVVQSDGFV 180 MKN+MDE+QLDYTGSEL VAAFCRVFDMNVLAYARNA+VQSDGFV Sbjct: 127 MKNRMDENQLDYTGSELKGKTMGIIGLGAIGKKVAAFCRVFDMNVLAYARNALVQSDGFV 186 Query: 181 KMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTARGGIVNERDLIDA 240 KMTDFDTL+STSDI+SVH+PLNQQTKQLINKA FKKMKN+ LFINTARGGIVNERDLIDA Sbjct: 187 KMTDFDTLVSTSDIVSVHVPLNQQTKQLINKAAFKKMKNTALFINTARGGIVNERDLIDA 246 Query: 241 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR 300 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR Sbjct: 247 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR 306 Query: 301 IENGEEPESKLNQLL 315 +ENGEEPESKLNQLL Sbjct: 307 VENGEEPESKLNQLL 321 >A6LZ51.1 A6LZ51_CLOB8 D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding Length = 320 Score = 504 bits (1297), Expect = e-141, Method: Compositional matrix adjust. Identities = 240/314 (76%), Positives = 264/314 (84%) Query: 1 MKILIVGYFNETSKSNIARYFPQDWNVVVVPPGQEMLHHIEDCQVIIPEHIKVDHSLLSI 60 MKILIVGYF E SKSNI R+FP+DW++V+V PG+EMLHHIEDCQV+IPEHIKVD SLLSI Sbjct: 7 MKILIVGYFTEASKSNIVRHFPEDWDIVIVQPGKEMLHHIEDCQVLIPEHIKVDCSLLSI 66 Query: 61 AKKLKLVQTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSYYKNIPFLDTF 120 AKKLKLVQTGAGFDNVD+ ACTQ GIW EHVMALILSYYKNIPFLD+F Sbjct: 67 AKKLKLVQTGAGFDNVDIDACTQYGIWAANAAGVNAQAVAEHVMALILSYYKNIPFLDSF 126 Query: 121 MKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAYARNAVVQSDGFV 180 +KNK+DE++L YTGSEL VA FCRVFDMN+L YARN VVQSD FV Sbjct: 127 IKNKIDENELQYTGSELKGKTIGIIGFGAVGKKVAEFCRVFDMNILVYARNPVVQSDSFV 186 Query: 181 KMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTARGGIVNERDLIDA 240 KMTDFDTL+ SDI+SVH+ LNQQTKQLINK VFKKMKN+ LF+NTARGGIVNERDLIDA Sbjct: 187 KMTDFDTLVGASDIVSVHVSLNQQTKQLINKDVFKKMKNTALFVNTARGGIVNERDLIDA 246 Query: 241 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR 300 LKN DISGACLDVFESEPLPI+SELRNL NVILTPHTAG+PDG KFHKKRYDFF+NNI R Sbjct: 247 LKNKDISGACLDVFESEPLPIESELRNLRNVILTPHTAGLPDGLKFHKKRYDFFVNNINR 306 Query: 301 IENGEEPESKLNQL 314 +ENGEEP+SKLNQL Sbjct: 307 VENGEEPKSKLNQL 320 >C2BXQ9.1 C2BXQ9_LISGR NAD binding D-isomer specific 2-hydroxyacid dehydrogenase Length = 318 Score = 386 bits (991), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/312 (58%), Positives = 226/312 (72%) Query: 1 MKILIVGYFNETSKSNIARYFPQDWNVVVVPPGQEMLHHIEDCQVIIPEHIKVDHSLLSI 60 MK+LIVG F + +K I FP+ W VV++PPG+EM+ H+EDC+V+IPEHI+VD LL+ Sbjct: 5 MKVLIVGEFTKQTKEIILSLFPKKWAVVILPPGEEMMKHLEDCEVLIPEHIQVDEELLAT 64 Query: 61 AKKLKLVQTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSYYKNIPFLDTF 120 AK+LK +QTGAGFDNVDV ACT+ G+ EHVMAL+L+Y+KNIP LD F Sbjct: 65 AKELKFIQTGAGFDNVDVDACTKFGVRVANAAGVNAQAVAEHVMALMLAYFKNIPQLDAF 124 Query: 121 MKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAYARNAVVQSDGFV 180 MKN+ DE++L Y GSEL VAAFC+ F M V AYARN + G V Sbjct: 125 MKNREDENRLSYAGSELEGKTIGIIGMGAVGKKVAAFCQAFGMKVFAYARNTAGTAPGSV 184 Query: 181 KMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTARGGIVNERDLIDA 240 KM FDTL+ +DIISVH+ LNQQTK+LI+ A FKKMK + + INTARGGI+ E+DLI+A Sbjct: 185 KMCSFDTLVREADIISVHVSLNQQTKKLIDAATFKKMKKNAVVINTARGGILAEQDLIEA 244 Query: 241 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR 300 LK G+I+GACLDVFE EPL DS LR++ NVILTPHTAGMPDG K H+KRY FF+ NI+R Sbjct: 245 LKTGEIAGACLDVFEEEPLAKDSVLRDMQNVILTPHTAGMPDGYKIHQKRYAFFVANIER 304 Query: 301 IENGEEPESKLN 312 I GE+P++ LN Sbjct: 305 IVKGEQPQNNLN 316 >C2ESK5.1 C2ESK5_9LACO NAD binding D-isomer specific 2-hydroxyacid dehydrogenase Length = 309 Score = 282 bits (722), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 145/312 (46%), Positives = 193/312 (61%), Gaps = 3/312 (0%) Query: 1 MKILIVGYFNETSKSNIARYFPQDWNVVVVPPGQEMLHHIEDCQVIIPEHIKVDHSLLSI 60 M +LI+G F ++K I + FPQ+W V + L+ + + IIPEHI V+ SLL Sbjct: 1 MNVLIIGDFPVSTKQKIYKVFPQEWQVHITNLRSAPLY-LSSAEAIIPEHIPVNSSLLKQ 59 Query: 61 AKKLKLVQTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSYYKNIPFLDTF 120 A +LK++QTGAG+DNVDV CT GI EH +ALILS++KNIP+LD F Sbjct: 60 APQLKIIQTGAGYDNVDVAECTHRGIKVCNAAGVNANAVVEHTLALILSWFKNIPYLDCF 119 Query: 121 MKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAYARNAVVQSDGFV 180 MK++ SQL Y G+E+ +A +MNVLAY N + V Sbjct: 120 MKDQQPASQLTYNGAEIAGKTLGIVGLGNVGARMAHIGLALNMNVLAY--NHHPRHISGV 177 Query: 181 KMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTARGGIVNERDLIDA 240 +M D TL SDIIS+HI T LIN F++MK+S L +NT+RG IV+E LI A Sbjct: 178 RMVDLPTLYKNSDIISLHIAATPATHHLINSQAFQQMKSSALLVNTSRGSIVDEAALISA 237 Query: 241 LKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFFINNIKR 300 L+ +I+GACLDVFE EPLP+DS LR+L NVILTPHTAG+PD KFH++RY+FF N+ R Sbjct: 238 LQTHEIAGACLDVFEQEPLPMDSPLRHLSNVILTPHTAGLPDSVKFHRRRYEFFKENLTR 297 Query: 301 IENGEEPESKLN 312 GE+P++ +N Sbjct: 298 FAKGEKPKNLVN 309 >O29445.1 SERA_ARCFU D-3-phosphoglycerate dehydrogenase EC=1.1.1.95 Length = 527 Score = 158 bits (399), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 91/250 (36%), Positives = 136/250 (54%), Gaps = 7/250 (2%) Query: 34 QEMLHHIEDCQ-VIIPEHIKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXX 91 +E++ + + +++ KVD ++ AK LK++ + G G DN+D+ A TQ GI Sbjct: 33 EELIREVPKYEAIVVRSQTKVDAEVIQAAKNLKIIGRAGVGVDNIDINAATQRGIVVVNA 92 Query: 92 XXXXXXXXXEHVMALILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXX 151 EH +AL+L+ + IP D +K E + + G EL Sbjct: 93 PGGNTISTAEHAIALMLAAARKIPQADRSVKEGKWERK-KFMGIELRGKTAGVIGLGRVG 151 Query: 152 XXVAAFCRVFDMNVLAY---ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQL 208 VA C+ +MNVLAY + G VK+ DFDTLL++SD+I+VH+P ++T L Sbjct: 152 FEVAKRCKALEMNVLAYDPFVSKERAEQIG-VKLVDFDTLLASSDVITVHVPRTKETIGL 210 Query: 209 INKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNL 268 I K F+KMK+ + +N ARGGIV+E L +A+K G ++ A LDV+E EP D+ L L Sbjct: 211 IGKGQFEKMKDGVIVVNAARGGIVDEAALYEAIKAGKVAAAALDVYEKEPPSPDNPLLKL 270 Query: 269 GNVILTPHTA 278 NV+ TPH A Sbjct: 271 DNVVTTPHIA 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,657,469,594 Number of extensions: 61767664 Number of successful extensions: 148030 Number of sequences better than 1.0e-05: 6401 Number of HSP's gapped: 143593 Number of HSP's successfully gapped: 6403 Length of query: 315 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 180 Effective length of database: 1,788,859,178 Effective search space: 321994652040 Effective search space used: 321994652040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)