BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0015.p (549 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFS5.1 B3KFS5_9BACI Extracellular solute-binding protein family 1 136 5e-30 Q65FY5.1 Q65FY5_BACLD YtcQ 122 1e-25 B3KF76.1 B3KF76_9BACI Extracellular solute-binding protein family 1 120 4e-25 A9KLI6.1 A9KLI6_CLOPH Extracellular solute-binding protein family 1 116 7e-24 B1DB34.1 B1DB34_9BACL Extracellular solute-binding protein family 1 116 7e-24 >B3KFS5.1 B3KFS5_9BACI Extracellular solute-binding protein family 1 Length = 519 Score = 136 bits (343), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 126/469 (26%), Positives = 227/469 (48%), Gaps = 39/469 (8%) Query: 81 MHAIEQKLNIKIVPEMVDVQQYKTKLNLKIASGAIPDVVRIDFADDFQKYAQQGAFVDLT 140 ++ IE++ ++I + + Y KL++ ++SG +PD++ F Y +Q AF+ L Sbjct: 87 LNYIEERTGLEIGVNLPPPEVYNEKLDVIMSSGNLPDMLHTYEPVWFDNYVKQKAFMPLD 146 Query: 141 DLINEQDTPNIMREVPQDVFEKAKVNGKIYGIP-YQGGPGAGYRWNLDMRKDYLEKVGAD 199 +LI+E P++ ++P +V+++ + GKIY +P GA + +RKD+L+++G Sbjct: 147 ELIDEY-GPHLKEKIPPEVWDRVRYGGKIYAVPSLNEVTGAELMY---VRKDWLDRLGLQ 202 Query: 200 IPKTLDEYYNLLKKIKTEMPDVIPLGGYTAQIGEQKYAN-NSFDHIFGAFGVTPGFFTEK 258 P+TL+EYY +++ + PD G IG ++ FGAFG + E+ Sbjct: 203 PPETLEEYYEVIRAFTLDDPDG---NGVQDTIGLTLTSSLGRSSPFFGAFGTQRNTWFER 259 Query: 259 DGKFSNYDIEPQMREALLFLQKMYAEGLIDKEFPTIKEEQERTKLYSGKLFSWMGWWSTP 318 DG+ N I P+ +EAL F K+Y E L+D+EFP + K+ +G + + W Sbjct: 260 DGQLVNGSILPETKEALAFFAKLYQENLLDREFPLNLQNNLFEKIENGTVGLFSAAW--- 316 Query: 319 NGYDTQIETGELIKSGSLAKDGTLPDQANEPYKYLALTDSLVGPDGTAVAPMGAPFSQMN 378 YDT+ G + + +++KD P+ P Y D G +G + N Sbjct: 317 --YDTR---GPI--AANMSKD---PEAEWMPLPYPTGPDGFKGVEGKDI------IRGYN 360 Query: 379 AISVKTKDPKRVLKIIEDSLSLENQMLTVWGIEGEDYKIENGVLKTVTDLVDPQTMKDKD 438 I +++ V++ + D ++ + + L + G E E ++ E+G + VTD + DK Sbjct: 361 VIPAESEKAAEVIRFL-DFIASDYKTLKL-GFENEIWRWEDGAI--VTDF----ALHDKH 412 Query: 439 GNYRGTQSYLFAPGLTGWXXXXXXXXXXXXXXXGIAINNKFQITDASNYLDSPTKIAKLV 498 YRG L + + N+ I +A + +P Sbjct: 413 L-YRGIYQSLVDVPDQLLFKERLDSLGDFNLYNNLQLINQHIIPNAFYGVPTPGMTKYNT 471 Query: 499 ELNTMRDSVFTQIIMGGD-IKKFDEFVEKWKAQGGAKILQELEESFQKK 546 +L + D VFT+IIMG + + FD++V+ WK +GG +I +E+ E + K+ Sbjct: 472 KLKNLED-VFTKIIMGLEPLDAFDDYVKTWKEEGGDEITKEVNEWYSKQ 519 >Q65FY5.1 Q65FY5_BACLD YtcQ Length = 501 Score = 122 bits (306), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 139/513 (27%), Positives = 234/513 (45%), Gaps = 67/513 (13%) Query: 51 EPLAAGEPYVLNWMKAQDISKPYDPAKDVVMHAIEQKLNIKI----VPEMVDVQQYKTKL 106 E + P L+WM I P KD + IE+ N K+ VP+ V + +L Sbjct: 35 EKVDLNSPLDLSWMV---ILYHQQPPKDTAIKKIEELTNTKLDLMWVPDAVK----EDRL 87 Query: 107 NLKIASGAIPDVVRI-DFADDFQKYA-QQGAFVDLTDLINEQDTPNIMREVPQDVFEKAK 164 N +A+G +P VV I D + A + G F ++ + +D PN+ + + + + + A Sbjct: 88 NSALAAGNLPKVVTIQDIKNSSVMNAFRSGMFWEVGPYL--KDYPNLSK-MNKLINKNAS 144 Query: 165 VNGKIYGIPYQGGPGAGYRWNLDMRKDYLEKVGADIPKTLDEYYNLLKKIKTEMPDVIPL 224 ++GK+YGI Y+ P + R + +RKD+L+ +G D+PKT+DE Y K + PD Sbjct: 145 IDGKLYGI-YRERPLS--RQGIVIRKDWLDNLGLDMPKTVDELYETAKAFTEKDPDQ--- 198 Query: 225 GGYTAQIGEQKYANNSFDHIFGAFGV------TPGFFTEKDGKFSNYDIEPQMREALLFL 278 G IG +A+ + D I+GAF P + EKDGKF+ + + + + ++ Sbjct: 199 NGKNDTIG---FADRN-DLIYGAFKTLGSYMGMPTDWEEKDGKFTPDFMTKEYMDTMKYM 254 Query: 279 QKMYAEGLIDKEFPTIKEEQERTKLYSGKLFSWMGWWSTPNGYDTQIETGELIKSGSLAK 338 +K+Y EGLI+K+FP + Q++ GK ++ G++ Sbjct: 255 KKLYDEGLINKDFPVTSKTQQQEMFSQGKAGIYV---------------------GNMVD 293 Query: 339 DGTLPDQANEPYKYLALTDSLVGPDGTAVAPMGAPFSQMNAI---SVKT-KDPKRVLKII 394 L DQ+ + L + + + GPDG + + A SVKT K+ KR+L Sbjct: 294 AVNLRDQSVDDKMELEIINRIKGPDGKERVWASGGHNGIFAFPKTSVKTEKELKRILAFF 353 Query: 395 EDSLSLENQMLTVWGIEGEDYKIE-NGVLKTVTDLVDPQTMKDKDGNYRGTQSYLFAPGL 453 + + L +GIEG YK E N K + + +KD + S + G+ Sbjct: 354 DRIAEEDVYSLMTYGIEGVHYKKEGNKQFKRID-----EKLKDWQAEIQPLVSLV---GI 405 Query: 454 TGWXXXXXXXXXXXXXXXGIAINNKFQITDASNYLDSPTKIAKLVELNTMRDSVFTQIIM 513 NN+ +++ + L SP + EL + D Q I+ Sbjct: 406 DKQYLKNTGDPLRTKYEELTEDNNEIIVSNPAESLYSPAASERGDELQKIIDDATYQFIL 465 Query: 514 GG-DIKKFDEFVEKWKAQGGAKILQELEESFQK 545 G + +F++ VEKW+ GG ++++E EESF+K Sbjct: 466 GKINESQFEKAVEKWRTNGGNQVIEEYEESFKK 498 >B3KF76.1 B3KF76_9BACI Extracellular solute-binding protein family 1 Length = 504 Score = 120 bits (301), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 106/355 (29%), Positives = 170/355 (47%), Gaps = 43/355 (12%) Query: 80 VMHAIEQKLNIKIVPEMVDVQQYKTKLNLKIASGAIPDVVRI--DFADDFQKYAQQGAFV 137 V +E+K K+ +M+ ++ KLNL ++SG D+V D A + YAQ+GA V Sbjct: 62 VAKLLEEKTGYKVQYDMLPQDKWAEKLNLIMSSGEAYDIVTSYNDMAL-YSDYAQKGALV 120 Query: 138 DLTDLINEQDTPNIMREVPQDVFEKAKVNGKIYGIPYQGGPGAGYRWN--LDMRKDYLEK 195 DLT LI+E PNI + + E KVNGK+Y IP Y N + +R D+L+K Sbjct: 121 DLTPLIDEFG-PNIKAAIKPESMEALKVNGKLYAIPVT----VTYDINSSILIRTDWLDK 175 Query: 196 VGADIPKTLDEYYNLLKKIKTEMP-----DVIPLGGYTAQIGEQKYANNSFDHIFGAFGV 250 +G +P T +E N+L + K + P +PL G+Q + D++ GAFG+ Sbjct: 176 LGLSMPTTTEELTNVLTQFKEKDPGGNGDRNVPL----TIKGDQ----SMIDNLVGAFGI 227 Query: 251 TPGFFTEKDGKFSNYDIEPQMREALLFLQKMYAEGLIDKEFPTIKEEQERTKLYSGKLFS 310 + + + DGK ++P ++ + ++ +Y +GLID+EF K+ + K SG + Sbjct: 228 S-NTWNDVDGKLVPRVMDPAFKDYVAYVGNLYEKGLIDREFVANKDATAKEKFASGVAGA 286 Query: 311 WMGWWSTPNGYDTQIETGELIKSGSLAKDGTLPDQANEPYKYLALTDSLVGPDGTAVAPM 370 + W+ S SLAK +P A +L GP+G A Sbjct: 287 IVVHWADIPAI-----------SDSLAK--------TKPEAKFAFVPALKGPNGEAGFAA 327 Query: 371 GAPFSQMNAISVKTKDPKRVLKIIEDSLSLENQMLTVWGIEGEDYKIENGVLKTV 425 A F ++ I +K P+ +K I +SL E G+EG YK+ +G + + Sbjct: 328 NAGFDRLTFIPKASKHPEDAIKWINESLEPETFKNMAIGVEGTHYKVVDGAYEPI 382 >A9KLI6.1 A9KLI6_CLOPH Extracellular solute-binding protein family 1 Length = 544 Score = 116 bits (290), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 143/532 (26%), Positives = 231/532 (43%), Gaps = 83/532 (15%) Query: 57 EPYVLNWMKAQDISKPYDPAKDVVMHAIEQKLNIKIVPEMVDVQQYKTKLNLKIASGAIP 116 EP + W+ S+P + ++ IE+K N+K VD Q++ L K++SG +P Sbjct: 52 EPVTIEWLAYNTYSQPNTDTE--IVKQIEKKFNVKFEFWYVDDQKWDEILGAKLSSGDMP 109 Query: 117 DVVRIDFADDFQKYAQQGAFVDLTDLINEQDTPNIMREVPQDVFE-----KAKVNGKIYG 171 DV++I + Y +QG + TD + + P+ ++V + E A +GK Y Sbjct: 110 DVMKIKNTANIPTYVKQGILAEFTDEMLAK-IPSFTKQVEEANVEGNGLIDAYYDGKRYA 168 Query: 172 IPYQGGPGAGYRWNLDMRKDYLEKVGAD-IPKTLDEYYNLLKKIKTEMPDVIPLGGYTAQ 230 I G Y L R D+L+ +G + IP T+DE + I+ PD G Sbjct: 169 IKTPSISGT-YPTVLVWRTDWLKNLGIEKIPATIDEMEEAMYAIRNNDPDG---NGVKDT 224 Query: 231 IGEQKYANNSFDHIFGAFGVTPG-----------FFTEKDGKFSNYDIEPQMREALLFLQ 279 G +N + + +FGA+G P FFTEKDGK +P+M+ AL +Q Sbjct: 225 YG---MSNTAMNAVFGAYGAIPLKEFRGTGAQNLFFTEKDGKIEFACTQPEMKAALATIQ 281 Query: 280 KMYAEGLIDKEFPTIKEEQERTKLY--------SGKL----FSWMGWWSTPNGYDTQIET 327 K Y EGLID EF T E T Y +GK+ + W+ P +E Sbjct: 282 KWYKEGLIDPEFIT----GENTAGYWATSQAFENGKVGVTGMALASHWAPP------VEE 331 Query: 328 GELIKSGSLAKDGTL---PDQANEPYKYLALTDSLVGPDGTAVAPMGAPFSQMN-AISVK 383 G K G +G + PD E + + + ++ GP+G + FS I+ K Sbjct: 332 G---KKGGACYEGFVAMNPDAKWE--ETVNIGPAIQGPEGKSGTHTWGAFSPSGFGITTK 386 Query: 384 T-KDPKRV---LKIIED-SLSLENQMLTVWGIEGEDY-KIENGVLKTVTDLVDP-QTMKD 436 +DP++V L +IE S E +L WGIEG Y K E G ++ + P + ++D Sbjct: 387 CAEDPRKVDAILAMIEAYSSDPEYALLAGWGIEGTHYEKTEEGGVRRLEPFTKPSEYIQD 446 Query: 437 KDGNYRGTQSYLFAPGLTGWXXXXXXXXXXXXXXXGIAINNKFQITDASNYLDSPTKIAK 496 G + + F L+ ++K++ + L T+ A Sbjct: 447 GVGVFMLGTNTEFDRSLS---------------KNVFDFSDKYKTPGYQDILVPATEAAN 491 Query: 497 --LVELNTMRDSVFTQIIMGGD-IKKFDEFVEKWKAQGGAKILQELEESFQK 545 L +L + +I+ G + + FD FV+++ + GG +IL E+ K Sbjct: 492 QYLTDLKIFTLDAYIKIMTGEESVDYFDTFVKEFNSMGGEQILNEINAEIAK 543 >B1DB34.1 B1DB34_9BACL Extracellular solute-binding protein family 1 Length = 532 Score = 116 bits (290), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 127/481 (26%), Positives = 208/481 (43%), Gaps = 49/481 (10%) Query: 80 VMHAIEQKLNIKIVPEMVDVQQYKTKLNLKIASGAIPDVVRIDFADDFQKYAQQGAFVDL 139 V +EQK N+KI P V + IASG +PD+ + + Y QG + + Sbjct: 80 VQKWLEQKFNVKIKPVKV--------TDASIASGDVPDIFMLGDPANVSAYQNQGVLMSI 131 Query: 140 TDLINEQDTPNIMREVPQD--VFEKAKVNGKIYGIPY--QGGPGAGYRWNLDMRKDYLEK 195 ++ P ++ + +F+ N +++ IP P Y + RKD+L Sbjct: 132 DPNQLKEKMPEYYADIEKKSKLFQTVTFNDQLWAIPMFIDLKP---YDLGMLWRKDWLNN 188 Query: 196 VG-ADIPKTLDEYYNLLKKIKTEMPDVIPLGGYTAQIGEQKYANNS----FDHIFGAFGV 250 VG +P+TLDE+ + + PD G G A ++ F IFGAFGV Sbjct: 189 VGITKVPETLDEFEQAVYAFAQKDPDK---NGKKDTYGLTGTATSTWSSGFYSIFGAFGV 245 Query: 251 TPGFFTEKDGKFSNYDIEPQMREALLFLQKMYAEGLIDKEFPTIKEEQERTKLYSGKLFS 310 P + EK+G+ N + P+ +EAL L++ YA+G+ID EF T ++ R KLY+ ++ Sbjct: 246 EPTMWHEKNGEIVNGSVMPETKEALAKLRQWYADGVIDPEFITDTQDSYRKKLYNNRI-- 303 Query: 311 WMGWWSTPNGYDTQIETGELIKSGSLAKDGTLPDQANEPYKYLALTDSLVGPDGTAVAPM 370 + QI G L + G++ + +L P L + + GP G Sbjct: 304 --------GVIEEQIAKGALPEGGTVTEMKSL-----SPNAELVFSKNPKGPGGDGSWDW 350 Query: 371 GAPFSQMNAISVKTKD-PKRVLKIIE----DSLSLENQMLTVWGIEGEDYKI-ENGVLKT 424 G S I K KD P+++ K+ E +S E +T G++G + E+G Sbjct: 351 GVK-SNFVVIGSKVKDQPEKLDKLYEILRAESSDEETINMTSLGVKGTHWDFAESGAASG 409 Query: 425 VTDLVDPQTMKDKDGNYRGTQSYLFAPGLTGWXXXXXXXXXXXXXXXGIAINNKFQITDA 484 T + P K + + G + LF+ G ++ +TDA Sbjct: 410 ATKFL-PSFEKQEQRDQEGIR--LFSLGNITTQAYREKYSNPKLNEAIKTYSSAPNLTDA 466 Query: 485 SNYLDSPTKIAKLVELNTMRDSVFTQIIMGG-DIKKFDEFVEKWKAQGGAKILQELEESF 543 + P+ +L + F QII G + FD+FV +WKA+GG ++ +E E + Sbjct: 467 LLFSALPSDGKYKADLTALMQKYFAQIISGEIPLDDFDKFVTEWKARGGDELTKEANELY 526 Query: 544 Q 544 Q Sbjct: 527 Q 527 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,255,456,894 Number of extensions: 141261902 Number of successful extensions: 266620 Number of sequences better than 1.0e-05: 428 Number of HSP's gapped: 266334 Number of HSP's successfully gapped: 428 Length of query: 549 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 408 Effective length of database: 1,732,333,088 Effective search space: 706791899904 Effective search space used: 706791899904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)