BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0012.p (396 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D7M1.1 B1D7M1_9BACL Trans-2-enoyl-CoA reductase (NAD(+)) EC=1.... 562 e-158 B3KBG4.1 B3KBG4_9BACI Trans-2-enoyl-CoA reductase (NAD(+)) EC=1.... 513 e-143 B8I4V6.1 Y2273_CLOCE Putative reductase Ccel_2273 EC=1.3.1.- 496 e-138 A1ZZQ0.1 A1ZZQ0_9SPHI Trans-2-enoyl-CoA reductase EC=1.3.1.44 482 e-134 A6ECW5.1 A6ECW5_9SPHI Putative uncharacterized protein 478 e-133 >B1D7M1.1 B1D7M1_9BACL Trans-2-enoyl-CoA reductase (NAD(+)) EC=1.3.1.44 Length = 396 Score = 562 bits (1449), Expect = e-158, Method: Compositional matrix adjust. Identities = 264/394 (67%), Positives = 312/394 (79%) Query: 1 MIIQPKTRGFICTTAHPEGCAKQIQEQIDFVKAQKKLSGPVNVLVIGASTGYXXXXXXXX 60 MII+PK+RGFICTTAHP GCA+Q+ Q+++V++Q L GP NVLVIGASTGY Sbjct: 1 MIIKPKSRGFICTTAHPVGCAEQVNRQVEYVQSQPNLKGPRNVLVIGASTGYGLASRIVA 60 Query: 61 XXXXXXNTIGVFFDKAAEGTRTASAGWYNSAAFEKAAAQQGLKSHSIVGDAFSDAIKTKT 120 NT+GVF K AEG RTA GWYNSAAFEKAAA+ GLKS S+ GDAFSD K +T Sbjct: 61 AFGAGANTVGVFTSKGAEGNRTAKPGWYNSAAFEKAAAESGLKSFSLTGDAFSDETKART 120 Query: 121 IDLIKAEYGTVDLVVYSVASPRRTHPVTGETFSSVIKPVGAAYTNKTMNFHTGEVSTVTI 180 IDLI+ E G+VDLVVYSVA+PRR HP TGE +SVIKP+G +Y+NKT+NFH G VS TI Sbjct: 121 IDLIRTELGSVDLVVYSVAAPRRVHPKTGEVLNSVIKPIGQSYSNKTVNFHNGVVSEATI 180 Query: 181 EPATEDEVRQTIAVMGGEDWAMWIDQLQEAGVLADGATTVAYSYVGPKITHPIYRDGTIG 240 EPATE+E+ QTI VMGGEDW MWID LQ AGVLA+GATT+AYSY+GP+ITH +YRDGTIG Sbjct: 181 EPATEEEISQTITVMGGEDWQMWIDDLQAAGVLAEGATTIAYSYIGPEITHAVYRDGTIG 240 Query: 241 LAKNDLEQTAIALHEKLSAKGGRAFVSVNKALVTQSSSAIPVVPLYISTLYKVMKEKELH 300 AKNDLE TA L E+LS+ GGRA+V+V KALVTQSSSAIPVVPLYIS L+KVMKEK +H Sbjct: 241 RAKNDLEATAHNLTEQLSSTGGRAYVAVAKALVTQSSSAIPVVPLYISALFKVMKEKGIH 300 Query: 301 ENCIQQMYRLFTDHLYNGGEASADGSHLIRIDDWEMREDVQIEVIRRWNELETGNVPELT 360 E CI+QMYR+F LY G D LIR+DDWEMREDVQ V + W+E+ T NV EL+ Sbjct: 301 EGCIEQMYRMFATRLYVDGPTPVDEKGLIRMDDWEMREDVQAAVDKLWSEITTENVYELS 360 Query: 361 DLEGYREDFFHLFGFQTEGVDYEADTDPAVEIPN 394 DLEGYR +FF LFGF+T+G+DYEADT+P V++PN Sbjct: 361 DLEGYRREFFQLFGFETDGIDYEADTNPEVDVPN 394 >B3KBG4.1 B3KBG4_9BACI Trans-2-enoyl-CoA reductase (NAD(+)) EC=1.3.1.44 Length = 396 Score = 513 bits (1321), Expect = e-143, Method: Compositional matrix adjust. Identities = 250/396 (63%), Positives = 305/396 (77%) Query: 1 MIIQPKTRGFICTTAHPEGCAKQIQEQIDFVKAQKKLSGPVNVLVIGASTGYXXXXXXXX 60 MII+P+TRGFICTT+HP GC Q+QEQID+V Q + GP NVLVIGASTGY Sbjct: 1 MIIKPRTRGFICTTSHPVGCEAQVQEQIDYVLKQPGIEGPRNVLVIGASTGYGLSARIAA 60 Query: 61 XXXXXXNTIGVFFDKAAEGTRTASAGWYNSAAFEKAAAQQGLKSHSIVGDAFSDAIKTKT 120 NT+GV+ A RTASAGWYNSAAFEKAA GLKS+S+ GDAF+ K KT Sbjct: 61 AFGARANTVGVYRPSQATEKRTASAGWYNSAAFEKAADAAGLKSYSVTGDAFTQETKAKT 120 Query: 121 IDLIKAEYGTVDLVVYSVASPRRTHPVTGETFSSVIKPVGAAYTNKTMNFHTGEVSTVTI 180 ++LI+ E G VDLVVYSVAS RRT P TGET SSV+KP+G AYTNKT+NFHTGEV+ VTI Sbjct: 121 VELIRKELGQVDLVVYSVASARRTLPETGETVSSVLKPIGPAYTNKTVNFHTGEVTEVTI 180 Query: 181 EPATEDEVRQTIAVMGGEDWAMWIDQLQEAGVLADGATTVAYSYVGPKITHPIYRDGTIG 240 EPATE+EV+ T+ VMGG+DW +W+++L E GVLA TT+A+SY+G +IT IYRDG+IG Sbjct: 181 EPATEEEVKNTVEVMGGDDWELWMNELAEGGVLAPDVTTIAFSYIGSEITKAIYRDGSIG 240 Query: 241 LAKNDLEQTAIALHEKLSAKGGRAFVSVNKALVTQSSSAIPVVPLYISTLYKVMKEKELH 300 AK+ LE TA+ L+EKL+ GGRA+V+V+KALVTQSSSAIPVVPLY+S LYKVMKEK LH Sbjct: 241 QAKDHLEATALKLNEKLATGGGRAYVTVSKALVTQSSSAIPVVPLYVSALYKVMKEKGLH 300 Query: 301 ENCIQQMYRLFTDHLYNGGEASADGSHLIRIDDWEMREDVQIEVIRRWNELETGNVPELT 360 E CI+Q+YRLF+D LYNG E D IRIDDWE+REDVQ EV W+++ + N+ ELT Sbjct: 301 EGCIEQIYRLFSDRLYNGQETPVDEKGRIRIDDWELREDVQAEVAELWSKVTSENIEELT 360 Query: 361 DLEGYREDFFHLFGFQTEGVDYEADTDPAVEIPNQV 396 DL GYR +FF LFGF+T+GVDY+AD +P V+ P + Sbjct: 361 DLAGYRREFFQLFGFETDGVDYDADVNPVVDNPKAI 396 >B8I4V6.1 Y2273_CLOCE Putative reductase Ccel_2273 EC=1.3.1.- Length = 395 Score = 496 bits (1278), Expect = e-138, Method: Compositional matrix adjust. Identities = 233/392 (59%), Positives = 295/392 (75%), Gaps = 1/392 (0%) Query: 1 MIIQPKTRGFICTTAHPEGCAKQIQEQIDFVKAQKKLSGPVNVLVIGASTGYXXXXXXXX 60 MI+ PK RGFICTT+HP GC + Q++++K QKK++G VLVIGASTGY Sbjct: 1 MIVTPKFRGFICTTSHPVGCEYSVMNQVEYIKNQKKINGAKKVLVIGASTGYGLASRITA 60 Query: 61 XXXXXXNTIGVFFDKAAEGTRTASAGWYNSAAFEKAAAQQGLKSHSIVGDAFSDAIKTKT 120 TIG+FF++ +TASAGWYNSAAFEK A ++GL + SI GDAFS+ IK KT Sbjct: 61 AFGCGAATIGIFFERPGSKNKTASAGWYNSAAFEKMAKEEGLYAKSINGDAFSNEIKQKT 120 Query: 121 IDLIKAEYGTVDLVVYSVASPRRTHPVTGETFSSVIKPVGAAYTNKTMNFHTGEVSTVTI 180 IDLIK + G VD+VVYS+ASPRRTHPVTGE F+SVIKP+ AYT+KT++FHT VS TI Sbjct: 121 IDLIKKDLGKVDMVVYSLASPRRTHPVTGEVFNSVIKPIREAYTSKTVDFHTQLVSETTI 180 Query: 181 EPATEDEVRQTIAVMGGEDWAMWIDQLQEAGVLADGATTVAYSYVGPKITHPIYRDGTIG 240 EPA++DE+RQTIAVMGGEDW+MW+D L++A VL D T+AYSYVGP++TH +YR+GTIG Sbjct: 181 EPASDDEIRQTIAVMGGEDWSMWMDALKKADVLEDNVMTLAYSYVGPEVTHSVYREGTIG 240 Query: 241 LAKNDLEQTAIALHEKLSAKGGRAFVSVNKALVTQSSSAIPVVPLYISTLYKVMKEKELH 300 AK+DLE TAI ++E L GG+A+VS+NKA+VTQ+S+AIPV+ LY+S L+KVMKEK +H Sbjct: 241 KAKDDLEATAIKINENLRTIGGKAYVSINKAVVTQASAAIPVISLYVSALFKVMKEKNIH 300 Query: 301 ENCIQQMYRLFTDHLYNGGEASADGSHLIRIDDWEMREDVQIEVIRRWNELETGNVPELT 360 E CI+QMYR+F D LY+ G+ D I +DD EMR +VQ EV + WNE NV ++T Sbjct: 301 EGCIEQMYRMFNDRLYS-GDLKLDSKGRICMDDLEMRPEVQNEVTKLWNEANNDNVKDIT 359 Query: 361 DLEGYREDFFHLFGFQTEGVDYEADTDPAVEI 392 D+EGYR +FF LFGF+ E VDY AD D V I Sbjct: 360 DIEGYRREFFRLFGFEFEDVDYNADVDIEVNI 391 >A1ZZQ0.1 A1ZZQ0_9SPHI Trans-2-enoyl-CoA reductase EC=1.3.1.44 Length = 401 Score = 482 bits (1240), Expect = e-134, Method: Compositional matrix adjust. Identities = 228/393 (58%), Positives = 289/393 (73%), Gaps = 1/393 (0%) Query: 1 MIIQPKTRGFICTTAHPEGCAKQIQEQIDFVKAQKKLSGPVNVLVIGASTGYXXXXXXXX 60 M+I+P+TRGFIC TAHP+GC + + QI++++++ + GP VLVIGASTG+ Sbjct: 1 MVIEPRTRGFICLTAHPKGCEQNVINQINYIQSKGTIEGPKKVLVIGASTGFGLASRITA 60 Query: 61 XXXXXXNTIGVFFDKAAEGTRTASAGWYNSAAFEKAAAQQGLKSHSIVGDAFSDAIKTKT 120 TIGVF +K A +TASAGWYNSAAFEK A GL + SI GDAFS IK +T Sbjct: 61 AFGSNAATIGVFLEKPANRGKTASAGWYNSAAFEKQAQAAGLYAKSINGDAFSKEIKQQT 120 Query: 121 IDLIKAEYGTVDLVVYSVASPRRTHPVTGETFSSVIKPVGAAYTNKTMNFHTGEVSTVTI 180 +DLIKA+ G VDLVVYS+ASP RTHP TG S +KP+G ++ +KT++FHTG VS V I Sbjct: 121 LDLIKADLGQVDLVVYSLASPVRTHPETGVKHRSSLKPIGQSFADKTVDFHTGVVSEVAI 180 Query: 181 EPATEDEVRQTIAVMGGEDWAMWIDQLQEAGVLADGATTVAYSYVGPKITHPIYRDGTIG 240 EPA ED++ T+AVMGGEDW MW+D LQ+ +LA GAT VAYSY+GPK+T P+YR GTIG Sbjct: 181 EPANEDDIANTVAVMGGEDWEMWMDALQKENLLASGATAVAYSYIGPKLTEPVYRKGTIG 240 Query: 241 LAKNDLEQTAIALHEKLSAKGGRAFVSVNKALVTQSSSAIPVVPLYISTLYKVMKEKELH 300 AK+ LE TA + +KL A G+A+VSVNKALVTQ+SSAIPV+PLYIS LYK+MK + +H Sbjct: 241 RAKDHLEATAFTIADKLKAIDGKAYVSVNKALVTQASSAIPVIPLYISLLYKIMKAENIH 300 Query: 301 ENCIQQMYRLFTDHLYNGGEASADGSHLIRIDDWEMREDVQIEVIRRWNELETGNVPELT 360 E CI+QM+RLF D LYN A+ D IR+DDWEMREDVQ +V W ++ T +P L Sbjct: 301 EGCIEQMHRLFKDRLYNNKTAT-DAQGRIRVDDWEMREDVQAQVAELWTKVTTETLPALG 359 Query: 361 DLEGYREDFFHLFGFQTEGVDYEADTDPAVEIP 393 DL+GY +DFF+LFGF+ EGV+YE D + VEIP Sbjct: 360 DLKGYADDFFNLFGFRIEGVNYEEDLNEVVEIP 392 >A6ECW5.1 A6ECW5_9SPHI Putative uncharacterized protein Length = 396 Score = 478 bits (1231), Expect = e-133, Method: Compositional matrix adjust. Identities = 227/396 (57%), Positives = 289/396 (72%) Query: 1 MIIQPKTRGFICTTAHPEGCAKQIQEQIDFVKAQKKLSGPVNVLVIGASTGYXXXXXXXX 60 MII+PK RGFIC TAHPEGCA+ + QI++VK++ ++GP VLVIGASTG+ Sbjct: 1 MIIEPKIRGFICLTAHPEGCAQNVLNQINYVKSKGPVAGPKRVLVIGASTGFGLASRITS 60 Query: 61 XXXXXXNTIGVFFDKAAEGTRTASAGWYNSAAFEKAAAQQGLKSHSIVGDAFSDAIKTKT 120 +TIGV+F+K +TAS GWYN+AAFE+ A + GL + SI GDAFS+ IK KT Sbjct: 61 AFGSEASTIGVYFEKPPAPGKTASPGWYNTAAFEERAHEAGLYAKSINGDAFSNEIKQKT 120 Query: 121 IDLIKAEYGTVDLVVYSVASPRRTHPVTGETFSSVIKPVGAAYTNKTMNFHTGEVSTVTI 180 +DLIKA+ G +DL++YS+ASP+R HPVTG +SV++P+G +TNKT++FHTG VS V+I Sbjct: 121 MDLIKADLGQIDLIIYSLASPKRLHPVTGVVHNSVLRPIGDVFTNKTVDFHTGIVSEVSI 180 Query: 181 EPATEDEVRQTIAVMGGEDWAMWIDQLQEAGVLADGATTVAYSYVGPKITHPIYRDGTIG 240 +PA D++ T+AVMGG DWAMWIDQL+ AG+LA GATTVAYSY+GP +T +YR GTIG Sbjct: 181 QPAAGDDIENTVAVMGGSDWAMWIDQLKAAGLLAPGATTVAYSYIGPALTEAVYRKGTIG 240 Query: 241 LAKNDLEQTAIALHEKLSAKGGRAFVSVNKALVTQSSSAIPVVPLYISTLYKVMKEKELH 300 AK+ LE TA + + L G+AFVSVNKALVTQ+SSAIPV+PLYIS LYKVMKEK LH Sbjct: 241 AAKDHLEATAFTISDTLKDLDGKAFVSVNKALVTQASSAIPVIPLYISLLYKVMKEKGLH 300 Query: 301 ENCIQQMYRLFTDHLYNGGEASADGSHLIRIDDWEMREDVQIEVIRRWNELETGNVPELT 360 E CI+Q+ RLF D LY G D IR+DD EMREDVQ V W+E T ++P + Sbjct: 301 EGCIEQIQRLFADRLYTGASIPVDEKGRIRVDDLEMREDVQAHVAELWSEATTESLPVIG 360 Query: 361 DLEGYREDFFHLFGFQTEGVDYEADTDPAVEIPNQV 396 DL GY+ DF +LFGF EGVDY+ ++D V+I V Sbjct: 361 DLAGYKHDFLNLFGFDVEGVDYQKESDEMVQISGLV 396 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,267,208,380 Number of extensions: 91791571 Number of successful extensions: 221091 Number of sequences better than 1.0e-05: 316 Number of HSP's gapped: 220250 Number of HSP's successfully gapped: 316 Length of query: 396 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 258 Effective length of database: 1,760,596,133 Effective search space: 454233802314 Effective search space used: 454233802314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)