BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0011.p (413 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAV8.1 B3KAV8_9BACI Proteinase inhibitor I4 serpin 305 8e-81 Q3M416.1 Q3M416_ANAVT Proteinase inhibitor I4, serpin 264 1e-68 Q8YYR8.1 Q8YYR8_ANASP All0778 protein 257 1e-66 A0ZEH9.1 A0ZEH9_NODSP Proteinase inhibitor I4, serpin 253 2e-65 B4B059.1 B4B059_9CHRO Proteinase inhibitor I4 serpin 246 3e-63 >B3KAV8.1 B3KAV8_9BACI Proteinase inhibitor I4 serpin Length = 425 Score = 305 bits (780), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 165/388 (42%), Positives = 230/388 (59%), Gaps = 8/388 (2%) Query: 25 MSYSERQAAASKLDFRLAQQSNLLGLKLFSQLRDK---SGGNLTISPYSIATALALAYNG 81 +S ER+ K++ + + N GL+L L D + NL ISPYSI ALALAYNG Sbjct: 43 LSAQERKEVLGKVNAGMVKAQNDFGLRLHQALSDNPEYTSDNLIISPYSITQALALAYNG 102 Query: 82 SAGETAEELGQLLGYAPGELQKLNASHQALLPVMQDAGPGIELKLANSVWGTSRLPLRED 141 +AG+TA E+ +LG+ +N ++ L ++++ G G+ L +ANSVW L L+E Sbjct: 103 TAGDTAAEMADMLGWRGMNDAGINEGNRQLRSLLENGG-GVVLNVANSVWHRPGLKLKEA 161 Query: 142 YLKTGKAYYKAEIRTIDLAAPRSVTEINGWVSKHTEHTIKEMLDQPPGPDAVAVLVNALY 201 Y + K Y AE+R DL ++ EIN WV T I ++ +PPG VAVL+NA+Y Sbjct: 162 YAEAVKDSYGAEVRETDLQQEDAMHEINQWVKDKTSGMIPKIYHEPPG--GVAVLINAIY 219 Query: 202 FKGGWKDAFPEENTRQAEFHLPADETVQVPMMSRTGYFPYAAHEDWQAIRIPYGDEQMDM 261 F GGW D F NT EF L T QVPMM R + Y E WQAIRIPYGD +M M Sbjct: 220 FNGGWTDEFDPANTVDEEFTLTDGTTQQVPMMKREDRYSYKEGESWQAIRIPYGDGRMHM 279 Query: 262 LVILPSERSSLEDLEARLAGGSFPAEEDFENRQGTVGLPRFTANYGTDLKDAVQALGVKQ 321 LVILP E S+++ L +L + + D+ + LP+F A +L DA+++LG+ + Sbjct: 280 LVILPKESSTIDKLHRQLWKDASVWKGDYSFETVQLSLPKFKAEQSFELPDALKSLGMVK 339 Query: 322 AFDPAKGDFSKLADTPDPIYISRIIHKTYVDVNEKGTEASASTLVEMLAGAAPSVKLPFQ 381 AFD A+ DFSK+A+ D +I + H+T VDV+EKGT+A+A T V + A P+ P Sbjct: 340 AFDAAEADFSKMAEQGDGFWIDHVTHQTIVDVSEKGTKAAAVTAVGITESAEPTE--PIA 397 Query: 382 MTVDRPFLFVIEDRQTGVWLFLGAIENP 409 M ++RPF F IEDR TG WLF+G++ P Sbjct: 398 MNINRPFFFAIEDRDTGAWLFMGSVLKP 425 >Q3M416.1 Q3M416_ANAVT Proteinase inhibitor I4, serpin Length = 442 Score = 264 bits (674), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 140/398 (35%), Positives = 232/398 (58%), Gaps = 9/398 (2%) Query: 16 SGCSQGGTVMSYSERQAAASKLDFRLAQQSNLLGLKLFSQLRDKSGG--NLTISPYSIAT 73 S +Q G + S Q D ++ +N G KLFS++ + G N+++SP S+A Sbjct: 49 SALAQTGVSPAESSLQKKTVVPDTKIVDANNKFGFKLFSEIAKEDGNAKNISVSPTSVAI 108 Query: 74 ALALAYNGSAGETAEELGQLLGYAPGELQKLNASHQALLPVMQDAGPGIELKLANSVWGT 133 ALA+ YNG++G T + + + L L +LN+++ +L ++Q+ G ++L +ANS+W Sbjct: 109 ALAMTYNGASGSTQKAMAKTLELQGISLPELNSAYASLNKLLQNPGENVQLTIANSLWAN 168 Query: 134 SRLPLREDYLKTGKAYYKAEIRTIDLAAPRSVTEINGWVSKHTEHTIKEMLDQPPGPDAV 193 + LR D+L+T + +Y+A + +D +V IN WV ++T+ I +++D P+ Sbjct: 169 QDVSLRPDFLQTTRDFYQARVTNLDFKNVAAVNTINSWVKENTKGKIDKIVDNIE-PNQA 227 Query: 194 AVLVNALYFKGGWKDAFPEENTRQAEFHLPADETVQVPMMSRTGYFPYAAHEDWQAIRIP 253 L+NA+YF G W AF + T F+ + + Q PMMS+TG + Y +Q++ +P Sbjct: 228 LFLINAIYFNGKWSSAFDKSQTTPQTFYTASGQQKQHPMMSQTGEYRYYETPQFQSVSLP 287 Query: 254 YG-DEQMDMLVILPSERSSLEDLEARLAGGSFPA-EEDFENRQGTVGLPRFTANYGTDLK 311 YG D ++ + + LP++ S+L+ L ++ F NR+G++ LPRF NY L Sbjct: 288 YGQDGKVSLYIFLPTKNSNLKAFYQNLNAENWETWMPQFRNREGSIRLPRFQTNYDITLN 347 Query: 312 DAVQALGVKQAFDPAKGDFSKLADTPDPIYISRIIHKTYVDVNEKGTEASASTLVEMLAG 371 DA+++LG+ +AF K +FS + D + IS++ HKT +DVNE+GTEASA+T V M+A Sbjct: 348 DALKSLGMAEAFSD-KANFSGIGDN---LTISQVRHKTVIDVNEEGTEASAATSVGMVAT 403 Query: 372 AAPSVKLPFQMTVDRPFLFVIEDRQTGVWLFLGAIENP 409 + + PF+M VDRPF + I D Q+ LF+GA+ P Sbjct: 404 SLREPQQPFKMIVDRPFFYAIRDNQSKNILFMGAVVEP 441 >Q8YYR8.1 Q8YYR8_ANASP All0778 protein Length = 374 Score = 257 bits (657), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 132/376 (35%), Positives = 227/376 (60%), Gaps = 9/376 (2%) Query: 38 DFRLAQQSNLLGLKLFSQL--RDKSGGNLTISPYSIATALALAYNGSAGETAEELGQLLG 95 D ++ +N G KLFS++ +D + N+++SP S+A ALA+ YNG++G T + + + L Sbjct: 3 DTKIVDANNKFGFKLFSEIAKQDGNAKNISVSPTSVAIALAMTYNGASGSTQKAMAKTLE 62 Query: 96 YAPGELQKLNASHQALLPVMQDAGPGIELKLANSVWGTSRLPLREDYLKTGKAYYKAEIR 155 L +LN+ + +L ++Q+ G ++L +ANS+W + LR D+++T + +Y+A++ Sbjct: 63 LQGISLPELNSGYASLNKLLQNPGENVQLTIANSLWVNQDVSLRPDFVQTTRDFYQAKVT 122 Query: 156 TIDLAAPRSVTEINGWVSKHTEHTIKEMLDQPPGPDAVAVLVNALYFKGGWKDAFPEENT 215 +D +V IN WV ++T+ I +++D P+ L+NA+YF G W +AF + T Sbjct: 123 NLDFQNVTAVNTINSWVQENTKGKIDKIVDNIE-PNQALFLINAIYFNGKWSNAFDKSQT 181 Query: 216 RQAEFHLPADETVQVPMMSRTGYFPYAAHEDWQAIRIPYG-DEQMDMLVILPSERSSLED 274 F+ + + Q PMMS+TG + Y +Q++ +PYG D ++ + + LP++ S+L+ Sbjct: 182 TPQTFYTASGQQKQHPMMSQTGEYRYYETPQFQSVSLPYGQDGKVSLYIFLPTKNSNLKA 241 Query: 275 LEARLAGGSFPA-EEDFENRQGTVGLPRFTANYGTDLKDAVQALGVKQAFDPAKGDFSKL 333 L ++ F NR+G++ LPRF +Y L DA+++LG+ +AF K +FS + Sbjct: 242 FSQNLNAENWEKWMPQFRNREGSIRLPRFQTDYDITLNDALKSLGMAEAFS-NKANFSGI 300 Query: 334 ADTPDPIYISRIIHKTYVDVNEKGTEASASTLVEMLAGAAPSVKLPFQMTVDRPFLFVIE 393 D + IS++ HKT ++VNE+GTEASA+T V M+A + + PF+M VDRPF + I Sbjct: 301 GDN---LTISQVRHKTVIEVNEEGTEASAATSVGMVATSLREPQQPFKMIVDRPFFYAIR 357 Query: 394 DRQTGVWLFLGAIENP 409 D Q+ LF+GA+ P Sbjct: 358 DNQSKNILFMGAVVEP 373 >A0ZEH9.1 A0ZEH9_NODSP Proteinase inhibitor I4, serpin Length = 442 Score = 253 bits (647), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 144/407 (35%), Positives = 233/407 (57%), Gaps = 19/407 (4%) Query: 17 GCSQ--GGT-VMSYSERQAAASKL-------DFRLAQQSNLLGLKLFSQL--RDKSGGNL 64 GCSQ G T ++ SE+ + ++L D +L +N G KLFS++ S N+ Sbjct: 40 GCSQVSGNTNSLTQSEQPYSETRLPKKTLISDAKLVDANNKFGFKLFSEIWKNHSSQDNI 99 Query: 65 TISPYSIATALALAYNGSAGETAEELGQLLGYAPGELQKLNASHQALLPVMQDAGPGIEL 124 +SP S+A ALA+ YNG++G T + + + L L ++N+++ L ++++ P ++L Sbjct: 100 FVSPSSVAIALAMVYNGASGSTQQAMAKTLELQGMSLPEINSAYATLKELLENPDPQVQL 159 Query: 125 KLANSVWGTSRLPLREDYLKTGKAYYKAEIRTIDLAAPRSVTEINGWVSKHTEHTIKEML 184 +ANS+W L+ ++L++ + +YKA++ +D S IN WV T I +++ Sbjct: 160 TIANSLWANQEATLQPNFLQSTQDFYKAKVTNLDFKDAASANTINEWVKDSTRGKINKIV 219 Query: 185 DQPPGPDAVAVLVNALYFKGGWKDAFPEENTRQAEFHLPADETVQVPMMSRTGYFPYAAH 244 ++ PD V L+NA+YFKG W + F + T + F + E Q PMMS+TG + Y + Sbjct: 220 EKIE-PDQVLFLINAIYFKGKWSNQFDKSQTAKHPFTSISGEQKQHPMMSQTGEYRYYEN 278 Query: 245 EDWQAIRIPYG-DEQMDMLVILPSERSSLEDLEARLAGGSFPAE-EDFENRQGTVGLPRF 302 + +QA+ +PYG D ++ + LP E S+L+ L ++ F NR G + LPRF Sbjct: 279 DQFQAVSLPYGEDGKVSFYIFLPKEESNLQTFSENLNFDNWEKWLSQFRNRDGFIRLPRF 338 Query: 303 TANYGTDLKDAVQALGVKQAFDPAKGDFSKLADTPDPIYISRIIHKTYVDVNEKGTEASA 362 +Y L DA++ALG+ +AF + +FS + + IS + HKT+V+VNE+GTEA+A Sbjct: 339 KTDYDVTLNDALKALGMAEAF-TNQANFSGMGKN---LKISEVKHKTFVEVNEEGTEAAA 394 Query: 363 STLVEMLAGAAPSVKLPFQMTVDRPFLFVIEDRQTGVWLFLGAIENP 409 +T V M+ + PF+M VDRPF VI D QTG LF+G+I +P Sbjct: 395 ATSVGMVPTSFREKPEPFRMIVDRPFFSVIRDNQTGSILFMGSITDP 441 >B4B059.1 B4B059_9CHRO Proteinase inhibitor I4 serpin Length = 437 Score = 246 bits (628), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 129/372 (34%), Positives = 218/372 (58%), Gaps = 10/372 (2%) Query: 40 RLAQQSNLLGLKLFSQL-RDKSGGNLTISPYSIATALALAYNGSAGETAEELGQLLGYAP 98 +L + + GL LF L + N+ +SP S+A AL++ G+ G+T +++ LGY Sbjct: 72 QLIEANTQFGLNLFQTLVTEDPNQNVFVSPTSVAIALSMVLQGTDGKTQQQMLSTLGYEN 131 Query: 99 GELQKLNASHQALLPVMQDAGPGIELKLANSVWGTSRLPLREDYLKTGKAYYKAEIRTID 158 L+++N ++ LL +Q IE+ LAN+++ + R +LK + YY A+I +++ Sbjct: 132 LNLKQINMANLTLLKTLQTENSQIEVALANALYAREGVSFRHQFLKDNEQYYHAQITSLN 191 Query: 159 LAAPRSVTEINGWVSKHTEHTIKEMLDQPPGPDAVAVLVNALYFKGGWKDAFPEENTRQA 218 ++P++ IN W +HT I +++ + P V +L+NA+YFKG WK F ++ T++ Sbjct: 192 FSSPQAPGIINRWTKEHTNGKIDQIITRI-DPSEVLILINAIYFKGIWKTEFAQKQTKEE 250 Query: 219 EFHLPADETVQVPMMSRTGYFPYAAHEDWQAIRIPYGDEQMDMLVILPSERSSLEDLEAR 278 F+LP +T Q P+MS TG + Y +E++QA+ +PYGD ++ M V LP S+L + Sbjct: 251 PFYLPNGQTKQQPLMSGTGKYQYYENEEFQAVNLPYGDGRLSMYVFLPRTNSNLTKFLNQ 310 Query: 279 LAGGSFPA-EEDFENRQGTVGLPRFTANYGTDLKDAVQALGVKQAFDPAKGDFSKLADTP 337 + ++ F ++G++ +PRF Y T+LK+ + ALG++ F A +FS++ T Sbjct: 311 VTAENWTQWTGKFNVQEGSLKIPRFKLEYDTNLKETLSALGMESLFRQA--NFSQM--TS 366 Query: 338 DPIYISRIIHKTYVDVNEKGTEASASTLVEMLAGAAPSVKLPFQMTVDRPFLFVIEDRQT 397 P+ I+ + HKT+++VNEKGTEA+ T + + + F MTV+RPF I D QT Sbjct: 367 SPVVINEVKHKTFIEVNEKGTEAAGVTSIGVRGTSTAGT---FSMTVNRPFFCAIRDNQT 423 Query: 398 GVWLFLGAIENP 409 G LF+GAI +P Sbjct: 424 GTILFMGAIVDP 435 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,462,147,014 Number of extensions: 103701283 Number of successful extensions: 254658 Number of sequences better than 1.0e-05: 1876 Number of HSP's gapped: 247999 Number of HSP's successfully gapped: 1899 Length of query: 413 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 275 Effective length of database: 1,760,596,133 Effective search space: 484163936575 Effective search space used: 484163936575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)