BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0005.p (693 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFH6.1 B3KFH6_9BACI SpoIID/LytB domain protein 596 e-168 B1DDP1.1 B1DDP1_9BACL SpoIID/LytB domain protein 427 e-117 C0ZAN7.1 C0ZAN7_BREBN Putative uncharacterized protein 348 1e-93 Q5N097.1 Q5N097_SYNP6 Similar to stage II sporulation protein D 125 2e-26 Q31LM7.1 Q31LM7_SYNE7 Stage II sporulation protein D-like 123 8e-26 >B3KFH6.1 B3KFH6_9BACI SpoIID/LytB domain protein Length = 602 Score = 596 bits (1536), Expect = e-168, Method: Compositional matrix adjust. Identities = 316/612 (51%), Positives = 403/612 (65%), Gaps = 15/612 (2%) Query: 87 LDGYKVKVMDSPTWQAAAEAAKKLQSTSDKPMLFVASRNGATVYQLYTGPYTSESAAKEG 146 +D Y KV+++ W+AA+EAAKKLQ+TSDKPMLF + VY+LY G Y +E AA + Sbjct: 1 MDTYCSKVLETTDWKAASEAAKKLQATSDKPMLFADGK----VYKLYAGMYGTEKAAADA 56 Query: 147 LNRVSKAGLTLAEGQAPALVGSKHLSAGSFATQEEADAVAGTITAAGFDAWKTLVSGSDG 206 R +K GQ PA+ G+ HLSAGS+ ++ EA+ V AG DA+ +V + Sbjct: 57 AARTAKTAAAYLNGQTPAVKGALHLSAGSYGSETEAETVRKAYADAGLDAFNVMVPSGET 116 Query: 207 SAQVEVWVGEAANDSDLAALRT---AVTAGLPQLAVSAGAAATPGMMIRTDAGLDWSSES 263 SA EVWVGEAAN +DLAA++ V+ GL + A TPG++IR+D L+ ++ Sbjct: 117 SA-YEVWVGEAANTADLAAVKKEAAKVSGGLVEQN-----ANTPGLIIRSDVSLNLAAPQ 170 Query: 264 QAFHYQVSGTGAKFIVSGNS-AGIQLAERSKRVYRGNLELSALSGSLAVINEVPLEQYLY 322 + HY +SG + V G S AG + ERS R YRG LE+ SG L ++NE+P +QYLY Sbjct: 171 EVTHYMLSGNQSVLRVDGKSGAGTNVKERSDRSYRGALEIGQKSGQLYLVNELPFQQYLY 230 Query: 323 AVVGGEVSSGWPEEALKAQAVAARSYALSQGNRFDVANVVDTTLSQVYYGIGAEAPTITK 382 +VVG EV S W EALKAQAVAARSYAL QGN+F+VA+VVDTTLSQ YYG E P+I + Sbjct: 231 SVVGAEVPSSWGTEALKAQAVAARSYALFQGNKFEVAHVVDTTLSQAYYGTAYEYPSIIQ 290 Query: 383 AVDATAGEVLMSGGKIVEAVFSSNSGGVTADPSEVWGNGGATFVXXXXX-XXXXXXXXKK 441 AVDAT GEV+M GKIVE VFSSNSGG+TAD +EVWGN F K Sbjct: 291 AVDATDGEVVMKDGKIVETVFSSNSGGMTADSTEVWGNRNDLFAAVESPGDSSSQAALKA 350 Query: 442 WYYLLLDSGVSGYAREDNIQLTGGKTAAGLAIATASTKDVNIRPLPVVESSASPLGKLNP 501 WY++LL SGV+GY REDN++ TG T+AGL T + KD N+RPLP+++S+ P+ K+NP Sbjct: 351 WYHVLLPSGVTGYVREDNVKETGNATSAGLPYMTVTAKDTNVRPLPLIQSNVEPVSKMNP 410 Query: 502 GENAVVLDQFYENGSYSWVKGPYTSAELLKSLSGKXXXXXXXXXXXXXXXXRGPSGRVTQ 561 GE AVVLD+ E+ S+ WV+GPYTS +LL +L GK RGPSGR Sbjct: 411 GETAVVLDKVAESNSFEWVRGPYTSDQLLATLKGKTANSLPASITSLDVTERGPSGRALT 470 Query: 562 VKANGEILQVKYPDLFRSAFGGLPSTLFDIVPAGSYTVLGANGSTAAVNSSQSAGILXXX 621 VKANG++L VKYPD+FRSAF GLPSTLFDI GSYTVL ANG+ + V+S Q+ ++ Sbjct: 471 VKANGQVLDVKYPDMFRSAFNGLPSTLFDIESTGSYTVLSANGAVSTVSSGQTVSVISGS 530 Query: 622 XXXXXXXXXXVVMGADASSRVVTASSGFYFIGWGNGHGLGMSQWGVKGMADKGYDYKRIL 681 VVM ++RV+ +S GF F G G GHGLGMSQWG KGMAD+GYDYK+IL Sbjct: 531 GNGNVKGSGTVVMNGTGTARVIESSPGFKFTGKGFGHGLGMSQWGAKGMADEGYDYKQIL 590 Query: 682 QHYFNNVTINKE 693 QHY+ N TI K+ Sbjct: 591 QHYYRNTTITKD 602 >B1DDP1.1 B1DDP1_9BACL SpoIID/LytB domain protein Length = 701 Score = 427 bits (1099), Expect = e-117, Method: Compositional matrix adjust. Identities = 267/669 (39%), Positives = 379/669 (56%), Gaps = 18/669 (2%) Query: 37 TIRVALFADIGSKYKSTVPFVTLSS----TTGFSLMSGSAQLLPIPANNKIRVSLDGYKV 92 TIRVA++ + KY+ T SS T G S SG+ + AN + R++ D YKV Sbjct: 39 TIRVAIYLQLPGKYQVNTAAATFSSAGGLTIGMSDPSGTVPWINTGANVQSRIAKDDYKV 98 Query: 93 KVMDSPTWQAAAEAAKKLQSTSDKPMLFVASRNGATVYQLYTGPYTSESAAKEGLNR-VS 151 KV+++ ++ A K+LQ+ S + + G TVYQ+ G Y + + A + + ++ Sbjct: 99 KVIETESFDTALAVYKRLQALSGSGFITSLKKKGKTVYQVTEGTYGNAADATAAMTKWIA 158 Query: 152 KAGLTLAEGQAPALV-GSKHL-SAGSFATQEEADAVAGTITAAGFDAWKTLVSGSDGSAQ 209 + ++ G A A + G HL SA ++A++ EA + +AAG DA+ V +DGSA Sbjct: 159 DSTISKLIGSAKAAIQGPYHLESATTYASEAEAVKASDAFSAAGVDAF-VGVRKADGSAN 217 Query: 210 VE--VWVGEAANDSDLAALRTAVTAGLPQLAVSAGAAATPGMMIRTDAGLDWSSESQAFH 267 V V+VG AA+ ++L ++TA A L + +A ++ R D L ++S Sbjct: 218 VRYSVFVGTAASTAELNVVKTAAGAAGSGLKTADDSAY---LLERNDHTLSGKADSPITL 274 Query: 268 YQVSGTGAKF-IVSGNSAGIQLAERSKRVYRGNLELSALSGSLAVINEVPLEQYLYAVVG 326 Y AK I+ + I+L ERS R YRG E+S +G LAV+NE+P EQYLY+VVG Sbjct: 275 YAFPVNNAKVRIMPAGESTIKLTERSSRTYRGQFEVSEFNGKLAVVNELPFEQYLYSVVG 334 Query: 327 GEVSSGWPEEALKAQAVAARSYALSQGNRFDVANVVDTTLSQVYYGIGAEAPTITKAVDA 386 GE+ + WP EALKAQAV+AR+YAL QG F +ANVVD+ SQ Y G E P+ +AVD Sbjct: 335 GEMIASWPLEALKAQAVSARTYALYQGAGFQIANVVDSVSSQEYQGTVTEKPSTIQAVDE 394 Query: 387 TAGEVLMSGGKIVEAVFSSNSGGVTADPSEVWGNGGATF-VXXXXXXXXXXXXXKKWYYL 445 TAGEV++ GK++EA+FSSN GG TAD SE+WGN A WY + Sbjct: 395 TAGEVMLYNGKLIEALFSSNGGGKTADASEIWGNSVAYLKSVSSPTDSSSEKGLYNWYRV 454 Query: 446 LLDSGVSGYAREDNIQLTGGKTAAGLAIATASTKDVNIRPLPVVESSASPLGKLNPGENA 505 +L + +GY RED + +G AAG+ + T +T +R P VE + + +G++N G Sbjct: 455 VLPNNQTGYIREDLLTDSGQTNAAGVKLMTVNTDASKVRKNPKVEDTVALVGQVNKGTVV 514 Query: 506 VVLDQFYENGSYSWVKGPYTSAELLKSLSGKXXXXXXXXXXXXXXXXRGPSGRVTQVKAN 565 VLD+ EN + SWVKGP+TS+ELL +++ + GPSGRVTQ+ N Sbjct: 515 AVLDKAVENNAMSWVKGPFTSSELLATMTKQASVTGPITSLQVGTT--GPSGRVTQLLVN 572 Query: 566 GEILQVKYPDLFRSAFGGLPSTLFDIVPAGSYTVLGANGSTAAVNSS-QSAGILXXXXXX 624 G+ VKYPD+ RSA GGLPSTLF + T+LGANGST SS Q ++ Sbjct: 573 GQSYNVKYPDVLRSALGGLPSTLFKVDETAKVTMLGANGSTRTKTSSAQPVYMIQAGGKV 632 Query: 625 XXXXXXXVVMGADASSRVVTASSGFYFIGWGNGHGLGMSQWGVKGMADKGYDYKRILQHY 684 +VM D+ R T F F+G G GHG+G+SQ+G G+A +GYDY ILQ+Y Sbjct: 633 TQADSNVIVMNGDSQIRATTKDPSFRFVGSGYGHGIGLSQYGALGLAQQGYDYSYILQYY 692 Query: 685 FNNVTINKE 693 + +VTI KE Sbjct: 693 YKDVTIAKE 701 >C0ZAN7.1 C0ZAN7_BREBN Putative uncharacterized protein Length = 644 Score = 348 bits (892), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 228/670 (34%), Positives = 326/670 (48%), Gaps = 76/670 (11%) Query: 38 IRVALFADIGSKYKSTVPFVTLSSTTGFSLMSGSAQ---LLPIPANNKIRVSLDGYKVKV 94 IRVALF D G Y+ VP VTL++ G ++ + LP + R +D Y + V Sbjct: 36 IRVALFVDTGQGYRGVVPSVTLTAANGMEVVLNGPEGKTDLPELKGDAARYRVDEYHLIV 95 Query: 95 MDSPTWQAAAEAAKKLQSTSDKPMLFVASRNGATVYQLYTGPYTSESAAKEGLNRVSKAG 154 ++ W A + A++L + + VA RNG TVYQ+ +G Y + AA V++A Sbjct: 96 AETGDWNRAQQIAQQLSQRKYEGSIQVAKRNGQTVYQVVSGTYDTYQAAANQAKTVAQAI 155 Query: 155 LTLAEGQAPALVGSKHLSAGSFATQEEADAVAGTITAAGFDAWKTLVSGSDGSAQVEVWV 214 GQ P + G L AG F + +EA + AAG A+ +VS D + VW+ Sbjct: 156 -----GQNPLVKGPHRLEAGKFKSLKEAQEWQASFEAAGIPAYPVMVSEGD-KVKYAVWI 209 Query: 215 GEAANDSDLAALRTAVTAGLPQLAVSAGAAATPGMMIRTDAGLDWSSESQAFHYQVSGTG 274 G+ + L L+ A P + + + ++++ D + S + V Sbjct: 210 GDEVSPDALKELKRRANAAFPSFSYQEPDSHS-YVILKQDV---YGSSDEVIWKYVFSPQ 265 Query: 275 AKFIVSGNSAG----IQLAERSKRVYRGNLELSALSGSLAVINEVPLEQYLYAVVGGEVS 330 K V N A I + ER +R YRG +ELS +G L ++NE+P+E+YLY VVG E++ Sbjct: 266 VKLTVEANKADDEAVIGVVEREQRTYRGEMELSEYNGHLTLVNELPMEEYLYGVVGSEMA 325 Query: 331 SGWPEEALKAQAVAARSYALSQGNRFDVANVVDTTLSQVYYGIGAEAPTITKAVDATAGE 390 GWP EALK QAV +R+ A+ QGN++ VANV DT Q YYG E+ + +AVD T GE Sbjct: 326 PGWPIEALKTQAVLSRTRAVWQGNKYGVANVSDTVFEQAYYGYTKESDDVREAVDETEGE 385 Query: 391 VLMSGGKIVEAVFSSNSGGVTADPSEVWGNGGATFVXXXXXXXXXXXXXKKWYYLLLDSG 450 V+ GK E++F SN+GG+TAD +EVWGN WY + L G Sbjct: 386 VIYYQGKPAESLFYSNAGGMTADGTEVWGNPVPYLRSVESKDTYPMDNASVWYRVALADG 445 Query: 451 VSGYAREDNIQLTGGKTAAGLAIATASTKDVNIRPLPVVESSASPLGKLNPGENAVVLDQ 510 SGY R D + TG +AG +A ST++ N+R P ++ L L G +L + Sbjct: 446 TSGYVRNDLVTETGQSNSAGQRMAVISTENTNLRSGPST-TTNRVLSVLPTGAEVTILSE 504 Query: 511 FYENGSYSWVKGPYTSAELLKSLSGKXXXXXXXX----XXXXXXXXRGPSGRVTQVKANG 566 E +YSW +GP+T+ E+ ++ RGPSGRV ++ANG Sbjct: 505 VKEENAYSWTRGPFTATEIAAMINAAHDKNKAPRISGNVTSLKVTERGPSGRVIAMEANG 564 Query: 567 EILQVKYPDLFRSAF--GGLP--STLFDIVPAGSYTVLGANGSTAAVNSSQSAGILXXXX 622 + + V PD FRS F GG P ST F++ +N SQ Sbjct: 565 KPIVVSSPDAFRSVFQQGGSPLRSTKFEV---------------EKINGSQ--------- 600 Query: 623 XXXXXXXXXVVMGADASSRVVTASSGFYFIGWGNGHGLGMSQWGVKGMADKGYDYKRILQ 682 F F G G GHGLG+SQ+G + MA++GYDYK ILQ Sbjct: 601 --------------------------FIFRGTGFGHGLGVSQYGARAMAEEGYDYKDILQ 634 Query: 683 HYFNNVTINK 692 HY+ +VTI K Sbjct: 635 HYYQDVTIEK 644 >Q5N097.1 Q5N097_SYNP6 Similar to stage II sporulation protein D Length = 407 Score = 125 bits (313), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 69/131 (52%), Positives = 88/131 (67%), Gaps = 8/131 (6%) Query: 294 RVYRGNLELSALSGSLAVINEVPLEQYLYAVVGGEVSSGWPEEALKAQAVAARSYALSQG 353 R YRG L+L G++ IN V LE YLY+VVG EV + WP EALKAQAVAARS+ L + Sbjct: 108 RWYRGRLQLILDQGAVTAINHVGLESYLYSVVGSEVYASWPTEALKAQAVAARSFVLYRR 167 Query: 354 N-----RFDVANVVDTTLSQVYYGIGAEAPTITKAVDATAGEVLMSGGKIVEAVFSSNSG 408 + RFDV N +T QVY GI +EAPT AV+ATAG+VL +++EAVF ++SG Sbjct: 168 DRNRDPRFDVGN---STTWQVYKGIASEAPTTIAAVNATAGQVLTYNNRVIEAVFHASSG 224 Query: 409 GVTADPSEVWG 419 G T + +VWG Sbjct: 225 GHTENVEDVWG 235 Score = 60.5 bits (145), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 24/45 (53%), Positives = 32/45 (71%) Query: 647 SGFYFIGWGNGHGLGMSQWGVKGMADKGYDYKRILQHYFNNVTIN 691 S F IG G GHGLG+SQWG G+A +G DY+RIL H++ N ++ Sbjct: 343 SQFTIIGRGYGHGLGLSQWGAYGLASQGVDYRRILSHFYQNTNLS 387 >Q31LM7.1 Q31LM7_SYNE7 Stage II sporulation protein D-like Length = 407 Score = 123 bits (308), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 68/130 (52%), Positives = 87/130 (66%), Gaps = 8/130 (6%) Query: 294 RVYRGNLELSALSGSLAVINEVPLEQYLYAVVGGEVSSGWPEEALKAQAVAARSYALSQG 353 R YRG L+L G++ IN V LE YLY+VVG EV + WP EALKAQAVAARS+ L + Sbjct: 108 RWYRGRLQLILDQGAVTAINHVGLESYLYSVVGSEVYASWPTEALKAQAVAARSFVLYRR 167 Query: 354 N-----RFDVANVVDTTLSQVYYGIGAEAPTITKAVDATAGEVLMSGGKIVEAVFSSNSG 408 + RFDV N +T QVY GI +EAPT AV+ATAG+VL +++EAVF ++SG Sbjct: 168 DRNRDPRFDVGN---STTWQVYKGIASEAPTTIAAVNATAGQVLTYNNRVIEAVFHASSG 224 Query: 409 GVTADPSEVW 418 G T + +VW Sbjct: 225 GHTENVEDVW 234 Score = 60.5 bits (145), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 24/45 (53%), Positives = 32/45 (71%) Query: 647 SGFYFIGWGNGHGLGMSQWGVKGMADKGYDYKRILQHYFNNVTIN 691 S F IG G GHGLG+SQWG G+A +G DY+RIL H++ N ++ Sbjct: 343 SQFTIIGRGYGHGLGLSQWGAYGLASQGVDYRRILSHFYQNTNLS 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.128 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,667,902,986 Number of extensions: 141994269 Number of successful extensions: 344483 Number of sequences better than 1.0e-05: 442 Number of HSP's gapped: 344076 Number of HSP's successfully gapped: 471 Length of query: 693 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 550 Effective length of database: 1,713,491,058 Effective search space: 942420081900 Effective search space used: 942420081900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 135 (56.6 bits)