BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0004.p (342 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFH7.1 B3KFH7_9BACI S-adenosylmethionine--tRNA-ribosyltransfer... 536 e-150 B1DDP0.1 B1DDP0_9BACL S-adenosylmethionine--tRNA-ribosyltransfer... 486 e-135 C0ZAN8.1 QUEA_BREBN S-adenosylmethionine:tRNA ribosyltransferase... 461 e-128 Q9KDI6.1 QUEA_BACHD S-adenosylmethionine:tRNA ribosyltransferase... 452 e-125 C2RTL2.1 C2RTL2_BACCE S-adenosylmethionine:tRNA ribosyltransfera... 450 e-125 >B3KFH7.1 B3KFH7_9BACI S-adenosylmethionine--tRNA-ribosyltransferase-isomerase Length = 343 Score = 536 bits (1380), Expect = e-150, Method: Compositional matrix adjust. Identities = 259/343 (75%), Positives = 290/343 (84%), Gaps = 1/343 (0%) Query: 1 MNVDDYDFHLPEELIAQTPLSDRSASRLLLVNKENGQLEHRHFTDILEQFRPGDTLVLND 60 MNVD YDF LPE LIAQTPL DRS+SRLL +NK++GQ+ H+ F DI++ PGDTLVLND Sbjct: 1 MNVDLYDFELPEHLIAQTPLLDRSSSRLLTLNKKSGQISHQTFADIIDSLNPGDTLVLND 60 Query: 61 TRVIPARLFGVKEDTGAKAEVLLLKSLGEDRWEALVKPGKKLKTGAVIIFSE-ELKAVIE 119 T+VIPARLFG KEDTGAKAEVLLLK++GEDRWEALVKPGKKLK G VI F + +LKAVIE Sbjct: 61 TKVIPARLFGEKEDTGAKAEVLLLKNMGEDRWEALVKPGKKLKKGNVISFGDGKLKAVIE 120 Query: 120 DEADMGGRTLRFIYTGIFQEILDRLGSMPLPPYIKETLDDRERYQTVYAKHEGSAAAPTA 179 +E +MG RTL F Y GIFQEILD LG MPLPPYIKE LDDR+RYQTVYAKHEGSAAAPTA Sbjct: 121 EEGEMGARTLSFSYKGIFQEILDELGQMPLPPYIKEKLDDRDRYQTVYAKHEGSAAAPTA 180 Query: 180 GLHFTRELLEQIKAKGVTIAYITLHVGLGTFRPMSVEKVEEHVMHAEYFVLSQETADMLN 239 GLHFT LLEQI+AKGV IA+ITLHVGLGTFRPMSV++VE+HVMH EY+ LSQETAD+LN Sbjct: 181 GLHFTEALLEQIRAKGVDIAFITLHVGLGTFRPMSVDQVEDHVMHEEYYSLSQETADLLN 240 Query: 240 XXXXXXXXXXXVGTTSCRTLETAGRQAGGGPIQECSGWTDIFIYPGYTFTVVNALITNFH 299 VGTTSCRTLET G Q G P+ E SGWT IFIYPGY+FTVV+ALITNFH Sbjct: 241 ETKRRGGRVIAVGTTSCRTLETVGSQLKGEPLHESSGWTSIFIYPGYSFTVVDALITNFH 300 Query: 300 LPKSTLVMLVSALAGRENILAAYEAAIQQRYRFFSFGDAMFIY 342 LPKSTLVMLVSALAGRE+I+ AY+ A+ + YRFFSFGDAMFIY Sbjct: 301 LPKSTLVMLVSALAGREHIMHAYKEAVDKEYRFFSFGDAMFIY 343 >B1DDP0.1 B1DDP0_9BACL S-adenosylmethionine--tRNA-ribosyltransferase-isomerase Length = 348 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 233/348 (66%), Positives = 276/348 (79%), Gaps = 6/348 (1%) Query: 1 MNVDDYDFHLPEELIAQTPLSDRSASRLLLVNKENGQLEHRHFTDILEQFRPGDTLVLND 60 MNVD +DF LPE LIAQTPL+DR+ASRLL +NKE G++ HR FTD+ E GD LVLN+ Sbjct: 1 MNVDWFDFELPEHLIAQTPLADRTASRLLALNKETGEIAHRTFTDLAEYMNAGDVLVLNN 60 Query: 61 TRVIPARLFGVKEDTGAKAEVLLLKSLGEDRWEALVKPGKKLKTGAVIIFSEE------L 114 TRVIPARL GVK DTGAK E+LLLK L DRWE L KP K++K G+ + F + L Sbjct: 61 TRVIPARLTGVKPDTGAKIELLLLKQLEGDRWETLAKPAKRIKVGSELWFGDNGSGEPLL 120 Query: 115 KAVIEDEADMGGRTLRFIYTGIFQEILDRLGSMPLPPYIKETLDDRERYQTVYAKHEGSA 174 +A++EDE DMGGRT+RF Y GIFQE+LDRLG MPLPPYIKE L++RERYQTVYAKHEGSA Sbjct: 121 RAIVEDEGDMGGRTIRFRYEGIFQELLDRLGEMPLPPYIKERLEERERYQTVYAKHEGSA 180 Query: 175 AAPTAGLHFTRELLEQIKAKGVTIAYITLHVGLGTFRPMSVEKVEEHVMHAEYFVLSQET 234 AAPTAGLHFT+E L+Q+ KGV +AYITLHVGLGTFRPMSV+KVEEH MH+EY+ L ++ Sbjct: 181 AAPTAGLHFTKEFLQQLVDKGVKLAYITLHVGLGTFRPMSVDKVEEHEMHSEYYELDEQN 240 Query: 235 ADMLNXXXXXXXXXXXVGTTSCRTLETAGRQAGGGPIQECSGWTDIFIYPGYTFTVVNAL 294 A ++N VGTTS RTLET + GGPI+ C+GWTDIFI+PGY F +V+AL Sbjct: 241 AVIINEAKSSGARIIAVGTTSSRTLETVAARFEGGPIEACNGWTDIFIFPGYNFKLVDAL 300 Query: 295 ITNFHLPKSTLVMLVSALAGRENILAAYEAAIQQRYRFFSFGDAMFIY 342 +TNFHLPKSTLVMLVSALAGRE I+ AY A+++ YRFFSFGDAMFIY Sbjct: 301 LTNFHLPKSTLVMLVSALAGREAIMKAYGEAVREEYRFFSFGDAMFIY 348 >C0ZAN8.1 QUEA_BREBN S-adenosylmethionine:tRNA ribosyltransferase-isomerase EC=5.-.-.- Length = 342 Score = 461 bits (1185), Expect = e-128, Method: Compositional matrix adjust. Identities = 217/342 (63%), Positives = 268/342 (78%), Gaps = 2/342 (0%) Query: 1 MNVDDYDFHLPEELIAQTPLSDRSASRLLLVNKENGQLEHRHFTDILEQFRPGDTLVLND 60 M+V +DF LPE LIAQ PL DR++SRLL++ K+ G + H+ FT +++ + GD LV+ND Sbjct: 1 MDVQQFDFDLPEHLIAQHPLEDRTSSRLLVLEKQTGNIVHQQFTHLIDHLQAGDVLVMND 60 Query: 61 TRVIPARLFGVKEDTGAKAEVLLLKSLGEDRWEALVKPGKKLKTGAVIIFSEEL-KAVIE 119 +RV+PARL G K DTGAK E+LLLKSLGEDRWE LVKPGK++K G ++F L + V E Sbjct: 61 SRVLPARLIGEKTDTGAKIEILLLKSLGEDRWETLVKPGKRMKPGTEVVFGNGLLRCVCE 120 Query: 120 DEADMGGRTLRFIYTGIFQEILDRLGSMPLPPYIKETLDDRERYQTVYAKHEGSAAAPTA 179 D + GGR +RF+Y GIF EILD+LGSMPLPPYI L+D ERYQTVYAK GSAAAPTA Sbjct: 121 DVTETGGRIVRFLYEGIFYEILDQLGSMPLPPYIHAQLEDSERYQTVYAKERGSAAAPTA 180 Query: 180 GLHFTRELLEQIKAKGVTIAYITLHVGLGTFRPMSVEKVEEHVMHAEYFVLSQETADMLN 239 GLHFT+ LEQI AKGV +AY+TLHVGLGTFRP+S + VEEHVMHAEY+ + +ET +++N Sbjct: 181 GLHFTKPYLEQIAAKGVQLAYVTLHVGLGTFRPVSADSVEEHVMHAEYYEIPEETVELVN 240 Query: 240 XXXXXXXXXXXVGTTSCRTLETAGRQAGGGPIQECSGWTDIFIYPGYTFTVVNALITNFH 299 VGTTSCRTLET GR A GG + SGWTDIFIYPGYTF++++ L+TNFH Sbjct: 241 QAKAEGRRVIAVGTTSCRTLETVGR-ANGGKLVATSGWTDIFIYPGYTFSILDGLLTNFH 299 Query: 300 LPKSTLVMLVSALAGRENILAAYEAAIQQRYRFFSFGDAMFI 341 LPKSTLVMLVSALAG+EN++ AY+ A+++ YRFFSFGDAM I Sbjct: 300 LPKSTLVMLVSALAGKENVMRAYKRAVEEEYRFFSFGDAMLI 341 >Q9KDI6.1 QUEA_BACHD S-adenosylmethionine:tRNA ribosyltransferase-isomerase EC=5.-.-.- Length = 347 Score = 452 bits (1162), Expect = e-125, Method: Compositional matrix adjust. Identities = 223/343 (65%), Positives = 264/343 (76%), Gaps = 3/343 (0%) Query: 1 MNVDDYDFHLPEELIAQTPLSDRSASRLLLVNKENGQLEHRHFTDILEQFRPGDTLVLND 60 MNV D+DFHLPE+LIAQTPL DR+ASRLL+++K G +E + F D+L+ GD LVLND Sbjct: 1 MNVRDFDFHLPEKLIAQTPLKDRTASRLLVLDKHTGAVEDKKFPDLLQFIEAGDCLVLND 60 Query: 61 TRVIPARLFGVKEDTGAKAEVLLLKSLGEDRWEALVKPGKKLKTGAVIIFSE-ELKAVIE 119 TRV+PARLFGVKE+TGA+ EVLLLK + DRWE LVKP K+LK G +I F EL A Sbjct: 61 TRVLPARLFGVKEETGAQVEVLLLKQMEGDRWETLVKPAKRLKVGTIIRFGNGELTATCV 120 Query: 120 DEADMGGRTLRFIYTGIFQEILDRLGSMPLPPYIKETLDDRERYQTVYAKHEGSAAAPTA 179 E + GGR L F Y GIF E+LD+LG MPLPPYIKE LDD++RYQTV+AK+ GSAAAPTA Sbjct: 121 QELEHGGRVLEFSYEGIFHEVLDQLGEMPLPPYIKEQLDDKDRYQTVFAKNRGSAAAPTA 180 Query: 180 GLHFTRELLEQIKAKGVTIAYITLHVGLGTFRPMSVEKVEEHVMHAEYFVLSQETADMLN 239 GLHFT ELL+Q+K KGV +A+ITLHVGLGTFRP+SVE++EEH MHAE++ +S TA +LN Sbjct: 181 GLHFTEELLDQLKQKGVHLAFITLHVGLGTFRPVSVEEIEEHEMHAEFYQMSAGTARLLN 240 Query: 240 XXXXXXXXXXXVGTTSCRTLETAGRQAGGGPIQECSGWTDIFIYPGYTFTVVNALITNFH 299 VGTTS RTLET ++ E SGWT IFIYPGY F ++ LITNFH Sbjct: 241 EVKRQGGRIIAVGTTSARTLETIRQEH--DEFVETSGWTSIFIYPGYEFKGIDGLITNFH 298 Query: 300 LPKSTLVMLVSALAGRENILAAYEAAIQQRYRFFSFGDAMFIY 342 LPKSTLVMLVSALAGREN+L AY A++Q YRFFSFGDAMFIY Sbjct: 299 LPKSTLVMLVSALAGRENLLNAYAHAVKQSYRFFSFGDAMFIY 341 >C2RTL2.1 C2RTL2_BACCE S-adenosylmethionine:tRNA ribosyltransferase-isomerase Length = 350 Score = 450 bits (1158), Expect = e-125, Method: Compositional matrix adjust. Identities = 218/342 (63%), Positives = 261/342 (76%), Gaps = 2/342 (0%) Query: 1 MNVDDYDFHLPEELIAQTPLSDRSASRLLLVNKENGQLEHRHFTDILEQFRPGDTLVLND 60 M+++ +DFHLPEELIAQ PL DR SRL+++++E G +EH+HFTDIL GD LVLN+ Sbjct: 1 MDINLFDFHLPEELIAQVPLEDRETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNE 60 Query: 61 TRVIPARLFGVKEDTGAKAEVLLLKSLGEDRWEALVKPGKKLKTGAVIIFSE-ELKAVIE 119 T+V+PARL GVKEDTGA EVLLLK D+WE LVKP K++K G VI F E +LKA Sbjct: 61 TKVMPARLHGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCT 120 Query: 120 DEADMGGRTLRFIYTGIFQEILDRLGSMPLPPYIKETLDDRERYQTVYAKHEGSAAAPTA 179 AD GGR L F Y GIF EILD LG MPLPPYIKETL+DR+RYQTVYAK GSAAAPTA Sbjct: 121 GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA 180 Query: 180 GLHFTRELLEQIKAKGVTIAYITLHVGLGTFRPMSVEKVEEHVMHAEYFVLSQETADMLN 239 GLHFT ELLE++K KGV +A+ITLHVGLGTFRP+S + +EEH MHAEY+ +S+ETAD+LN Sbjct: 181 GLHFTEELLEKLKQKGVQLAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETADLLN 240 Query: 240 XXXXXXXXXXXVGTTSCRTLETAGRQAGGGPIQECSGWTDIFIYPGYTFTVVNALITNFH 299 VGTTS RTLET G + SGWTDIF+YPGY F ++ LITNFH Sbjct: 241 RVKENGGRIITVGTTSTRTLETIATDH-DGKLCAASGWTDIFMYPGYEFKAIDGLITNFH 299 Query: 300 LPKSTLVMLVSALAGRENILAAYEAAIQQRYRFFSFGDAMFI 341 LPKSTL+MLVSA A R+N+L AY A++++YRFFSFGDAMF+ Sbjct: 300 LPKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFV 341 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,940,149,946 Number of extensions: 76998297 Number of successful extensions: 186895 Number of sequences better than 1.0e-05: 1376 Number of HSP's gapped: 182214 Number of HSP's successfully gapped: 1427 Length of query: 342 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 206 Effective length of database: 1,779,438,163 Effective search space: 366564261578 Effective search space used: 366564261578 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)