BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0003.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFH8.1 B3KFH8_9BACI Queuine tRNA-ribosyltransferase EC=2.4.2.29 599 e-169 B1DDN9.1 B1DDN9_9BACL Queuine tRNA-ribosyltransferase EC=2.4.2.29 565 e-159 C3IPT2.1 C3IPT2_BACTU Queuine tRNA-ribosyltransferase 535 e-150 C3I6L6.1 C3I6L6_BACTU Queuine tRNA-ribosyltransferase 535 e-150 C3H6Q5.1 C3H6Q5_BACTU Queuine tRNA-ribosyltransferase 535 e-150 >B3KFH8.1 B3KFH8_9BACI Queuine tRNA-ribosyltransferase EC=2.4.2.29 Length = 379 Score = 599 bits (1544), Expect = e-169, Method: Compositional matrix adjust. Identities = 281/304 (92%), Positives = 296/304 (97%) Query: 2 AAITYEHIKTCKQSGARLGRVHTPHGVIETPAFMPVGTLATVKTMSPEELKQMDAHIILS 61 AA+TYEHIKTCKQSGARLGRVHTPHGVI+TP FMPVGTLATVKTMSPEELK MDAHIILS Sbjct: 3 AAVTYEHIKTCKQSGARLGRVHTPHGVIDTPTFMPVGTLATVKTMSPEELKAMDAHIILS 62 Query: 62 NTYHLFLRPGHDIIREAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVHFRSHLN 121 NTYHLFLRPGHDI++EAGGLHKFMNWDRPILTDSGGFQVFSL++MRKI+EEGVHFRSHLN Sbjct: 63 NTYHLFLRPGHDIVKEAGGLHKFMNWDRPILTDSGGFQVFSLAEMRKISEEGVHFRSHLN 122 Query: 122 GDKKFLSPEVAMEVQNALGSDIMMAFDECPPYPAEYDYVKKSLERTTRWAERCLKSHARP 181 GDK F+SPEVAME+QNALGSDIMMAFDECPPYPAEY+YVK+S ERT+RWAERCLKSHARP Sbjct: 123 GDKLFISPEVAMEIQNALGSDIMMAFDECPPYPAEYEYVKQSTERTSRWAERCLKSHARP 182 Query: 182 EDQGLFAIVQGGMYEDLRRQSAADLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLPQ 241 EDQ LFAIVQGGM+EDLRRQSA DLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLP Sbjct: 183 EDQALFAIVQGGMHEDLRRQSARDLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLPN 242 Query: 242 GKPRYLMGVGSPDALLEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDFGP 301 KPRYLMGVGSPDAL+EGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARD+GP Sbjct: 243 NKPRYLMGVGSPDALIEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDYGP 302 Query: 302 LDPE 305 LDPE Sbjct: 303 LDPE 306 >B1DDN9.1 B1DDN9_9BACL Queuine tRNA-ribosyltransferase EC=2.4.2.29 Length = 377 Score = 565 bits (1456), Expect = e-159, Method: Compositional matrix adjust. Identities = 270/303 (89%), Positives = 280/303 (92%) Query: 3 AITYEHIKTCKQSGARLGRVHTPHGVIETPAFMPVGTLATVKTMSPEELKQMDAHIILSN 62 A+ YE IK CKQSGARLGRVHTPHGVIETP FMPVGT ATVKTMSPEELK MDA IILSN Sbjct: 2 AVKYELIKVCKQSGARLGRVHTPHGVIETPTFMPVGTQATVKTMSPEELKTMDAQIILSN 61 Query: 63 TYHLFLRPGHDIIREAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVHFRSHLNG 122 TYHLFLRPGHDI+ AGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGV FRSHLNG Sbjct: 62 TYHLFLRPGHDIVGRAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVQFRSHLNG 121 Query: 123 DKKFLSPEVAMEVQNALGSDIMMAFDECPPYPAEYDYVKKSLERTTRWAERCLKSHARPE 182 DK F+SPE AME+QNALGSDIMMAFDECPPYPA+YDYVK+SLERTTRWAERCL SHAR Sbjct: 122 DKLFISPEKAMEIQNALGSDIMMAFDECPPYPADYDYVKQSLERTTRWAERCLNSHARKH 181 Query: 183 DQGLFAIVQGGMYEDLRRQSAADLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLPQG 242 DQGLFAIVQGGMY+DLR QSA DLTSMDFPGYAIGGLSVGE K LMYEVLDYTVP LP Sbjct: 182 DQGLFAIVQGGMYKDLRIQSAKDLTSMDFPGYAIGGLSVGEPKHLMYEVLDYTVPELPTT 241 Query: 243 KPRYLMGVGSPDALLEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDFGPL 302 KPRYLMGVGSPDAL+EGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNA++A DFGPL Sbjct: 242 KPRYLMGVGSPDALIEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAKFAEDFGPL 301 Query: 303 DPE 305 DPE Sbjct: 302 DPE 304 >C3IPT2.1 C3IPT2_BACTU Queuine tRNA-ribosyltransferase Length = 379 Score = 535 bits (1378), Expect = e-150, Method: Compositional matrix adjust. Identities = 247/305 (80%), Positives = 277/305 (90%) Query: 1 MAAITYEHIKTCKQSGARLGRVHTPHGVIETPAFMPVGTLATVKTMSPEELKQMDAHIIL 60 M AI YE IKTCKQ+GARLGRVHTPHG +TP FMPVGTLATVKTMSPEELK MD+ IIL Sbjct: 1 MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL 60 Query: 61 SNTYHLFLRPGHDIIREAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVHFRSHL 120 SNTYHL+LRPGH+IIREAGGLHKFMNWDR ILTDSGGFQVFSLSD R+I EEGVHFR+HL Sbjct: 61 SNTYHLWLRPGHEIIREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHL 120 Query: 121 NGDKKFLSPEVAMEVQNALGSDIMMAFDECPPYPAEYDYVKKSLERTTRWAERCLKSHAR 180 NGDK FLSPE AME+QNALGSDIMMAFDECPP+PA ++Y+KKS+ERT+RWAERCLK+H R Sbjct: 121 NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLKAHER 180 Query: 181 PEDQGLFAIVQGGMYEDLRRQSAADLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLP 240 P+DQGLF IVQGG YE+LRRQSA DL SMDFPGYA+GGLSVGE K +M VL++T PLLP Sbjct: 181 PQDQGLFGIVQGGEYEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP 240 Query: 241 QGKPRYLMGVGSPDALLEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDFG 300 KPRYLMGVGSPD+L++G+IRG+DMFDCVLPTRIARNGT MTS+GRLVV+NA++ARDFG Sbjct: 241 DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFG 300 Query: 301 PLDPE 305 PLDP Sbjct: 301 PLDPN 305 >C3I6L6.1 C3I6L6_BACTU Queuine tRNA-ribosyltransferase Length = 379 Score = 535 bits (1378), Expect = e-150, Method: Compositional matrix adjust. Identities = 247/305 (80%), Positives = 277/305 (90%) Query: 1 MAAITYEHIKTCKQSGARLGRVHTPHGVIETPAFMPVGTLATVKTMSPEELKQMDAHIIL 60 M AI YE IKTCKQ+GARLGRVHTPHG +TP FMPVGTLATVKTMSPEELK MD+ IIL Sbjct: 1 MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL 60 Query: 61 SNTYHLFLRPGHDIIREAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVHFRSHL 120 SNTYHL+LRPGH+IIREAGGLHKFMNWDR ILTDSGGFQVFSLSD R+I EEGVHFR+HL Sbjct: 61 SNTYHLWLRPGHEIIREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHL 120 Query: 121 NGDKKFLSPEVAMEVQNALGSDIMMAFDECPPYPAEYDYVKKSLERTTRWAERCLKSHAR 180 NGDK FLSPE AME+QNALGSDIMMAFDECPP+PA ++Y+KKS+ERT+RWAERCLK+H R Sbjct: 121 NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLKAHER 180 Query: 181 PEDQGLFAIVQGGMYEDLRRQSAADLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLP 240 P+DQGLF IVQGG YE+LRRQSA DL SMDFPGYA+GGLSVGE K +M VL++T PLLP Sbjct: 181 PQDQGLFGIVQGGEYEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP 240 Query: 241 QGKPRYLMGVGSPDALLEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDFG 300 KPRYLMGVGSPD+L++G+IRG+DMFDCVLPTRIARNGT MTS+GRLVV+NA++ARDFG Sbjct: 241 DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFG 300 Query: 301 PLDPE 305 PLDP Sbjct: 301 PLDPN 305 >C3H6Q5.1 C3H6Q5_BACTU Queuine tRNA-ribosyltransferase Length = 379 Score = 535 bits (1378), Expect = e-150, Method: Compositional matrix adjust. Identities = 247/305 (80%), Positives = 277/305 (90%) Query: 1 MAAITYEHIKTCKQSGARLGRVHTPHGVIETPAFMPVGTLATVKTMSPEELKQMDAHIIL 60 M AI YE IKTCKQ+GARLGRVHTPHG +TP FMPVGTLATVKTMSPEELK MD+ IIL Sbjct: 1 MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL 60 Query: 61 SNTYHLFLRPGHDIIREAGGLHKFMNWDRPILTDSGGFQVFSLSDMRKITEEGVHFRSHL 120 SNTYHL+LRPGH+IIREAGGLHKFMNWDR ILTDSGGFQVFSLSD R+I EEGVHFR+HL Sbjct: 61 SNTYHLWLRPGHEIIREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHL 120 Query: 121 NGDKKFLSPEVAMEVQNALGSDIMMAFDECPPYPAEYDYVKKSLERTTRWAERCLKSHAR 180 NGDK FLSPE AME+QNALGSDIMMAFDECPP+PA ++Y+KKS+ERT+RWAERCLK+H R Sbjct: 121 NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLKAHER 180 Query: 181 PEDQGLFAIVQGGMYEDLRRQSAADLTSMDFPGYAIGGLSVGESKQLMYEVLDYTVPLLP 240 P+DQGLF IVQGG YE+LRRQSA DL SMDFPGYA+GGLSVGE K +M VL++T PLLP Sbjct: 181 PQDQGLFGIVQGGEYEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP 240 Query: 241 QGKPRYLMGVGSPDALLEGSIRGVDMFDCVLPTRIARNGTTMTSQGRLVVRNAQYARDFG 300 KPRYLMGVGSPD+L++G+IRG+DMFDCVLPTRIARNGT MTS+GRLVV+NA++ARDFG Sbjct: 241 DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFG 300 Query: 301 PLDPE 305 PLDP Sbjct: 301 PLDPN 305 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,064,937,870 Number of extensions: 88605822 Number of successful extensions: 164611 Number of sequences better than 1.0e-05: 1709 Number of HSP's gapped: 162550 Number of HSP's successfully gapped: 1748 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)