BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0020.p (377 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5L2G4.1 Q5L2G4_GEOKA Cation efflux transporter 352 3e-95 C5D601.1 C5D601_9BACI Cation diffusion facilitator family transp... 352 3e-95 C0UXC6.1 C0UXC6_9BACT Cation diffusion facilitator family transp... 344 7e-93 A4IMT3.1 A4IMT3_GEOTN Cation efflux family protein 318 6e-85 B4BKM3.1 B4BKM3_9BACI Cation diffusion facilitator family transp... 318 6e-85 >Q5L2G4.1 Q5L2G4_GEOKA Cation efflux transporter Length = 315 Score = 352 bits (904), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 168/271 (61%), Positives = 208/271 (76%) Query: 107 MFLEFFGGLVTGSLALLSDSGHMLSDIASLALSLVAMIFAVKPPSLKNSYGYYRFEIMAA 166 M LEFFGGL+T SLALLSDSGHMLSD +SL LSLVA+ FA KP S +YG+YRFEI+AA Sbjct: 45 MLLEFFGGLITNSLALLSDSGHMLSDASSLVLSLVAIWFATKPASPNKTYGFYRFEILAA 104 Query: 167 LFNGVTLFVIAGFIIWEAWQRFFEPPAVASGTMMXXXXXXXXXXXXXXWSLMRKSDAKDN 226 LFNGVTLFVIAGFIIWEA +RF+ PP VASG+MM W+LM+K D K+N Sbjct: 105 LFNGVTLFVIAGFIIWEAIERFYNPPTVASGSMMLIASIGLFANLLSAWALMKKGDVKNN 164 Query: 227 INIRSAYLHIMXXXXXXXXXXXXXXIMNLFSWYVADPIISVLVALLILRGAWGVLKQAFH 286 +N+RSAYLH++ +M LFSWYVADPIIS+LVALLIL+GAWGV+K H Sbjct: 165 VNLRSAYLHVIGDALGSVGAIIAGLVMWLFSWYVADPIISILVALLILKGAWGVIKHTVH 224 Query: 287 ILMEGTPVSVNTNDIKAALLSIDGVEGVHDLHVWTITSGLDALSGHLLIKDHADPHSILQ 346 ILMEGTP++++ N++K AL SI+GV VHDLH+WTITSGLD+LS H+LI+DH D +LQ Sbjct: 225 ILMEGTPITIDQNEVKKALESIEGVINVHDLHIWTITSGLDSLSCHILIEDHQDSQKVLQ 284 Query: 347 QAINVVEDNFAIQHTTLQIENSSLKHGELRV 377 +AI+ +ED+F IQH T+QIE S + HGE++V Sbjct: 285 EAIHFIEDHFKIQHATIQIETSQIHHGEMKV 315 >C5D601.1 C5D601_9BACI Cation diffusion facilitator family transporter Length = 315 Score = 352 bits (904), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 168/271 (61%), Positives = 208/271 (76%) Query: 107 MFLEFFGGLVTGSLALLSDSGHMLSDIASLALSLVAMIFAVKPPSLKNSYGYYRFEIMAA 166 M LEFFGGL+T SLALLSDSGHMLSD +SL LSLVA+ FA KP S +YG+YRFEI+AA Sbjct: 45 MLLEFFGGLITNSLALLSDSGHMLSDASSLVLSLVAIWFATKPASPNKTYGFYRFEILAA 104 Query: 167 LFNGVTLFVIAGFIIWEAWQRFFEPPAVASGTMMXXXXXXXXXXXXXXWSLMRKSDAKDN 226 LFNGVTLFVIAGFIIWEA +RF+ PP VASG+MM W+LM+K D K+N Sbjct: 105 LFNGVTLFVIAGFIIWEAIERFYNPPTVASGSMMLIASIGLFANLLSAWALMKKGDVKNN 164 Query: 227 INIRSAYLHIMXXXXXXXXXXXXXXIMNLFSWYVADPIISVLVALLILRGAWGVLKQAFH 286 +N+RSAYLH++ +M LFSWYVADPIIS+LVALLIL+GAWGV+K H Sbjct: 165 VNLRSAYLHVIGDALGSVGAIIAGLVMWLFSWYVADPIISILVALLILKGAWGVIKHTVH 224 Query: 287 ILMEGTPVSVNTNDIKAALLSIDGVEGVHDLHVWTITSGLDALSGHLLIKDHADPHSILQ 346 ILMEGTP++++ N++K AL SI+GV VHDLH+WTITSGLD+LS H+LI+DH D +LQ Sbjct: 225 ILMEGTPITIDQNEVKKALESIEGVINVHDLHIWTITSGLDSLSCHILIEDHQDSQKVLQ 284 Query: 347 QAINVVEDNFAIQHTTLQIENSSLKHGELRV 377 +AI+ +ED+F IQH T+QIE S + HGE++V Sbjct: 285 EAIHFIEDHFKIQHATIQIETSQIHHGEMKV 315 >C0UXC6.1 C0UXC6_9BACT Cation diffusion facilitator family transporter Length = 342 Score = 344 bits (883), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 164/270 (60%), Positives = 204/270 (75%) Query: 107 MFLEFFGGLVTGSLALLSDSGHMLSDIASLALSLVAMIFAVKPPSLKNSYGYYRFEIMAA 166 M LEFFGGL+T SLALLSDSGHMLSD +SL LSLVA+ FA KP S +YG+YRFEI+AA Sbjct: 44 MLLEFFGGLITNSLALLSDSGHMLSDASSLVLSLVAIWFATKPASPNKTYGFYRFEILAA 103 Query: 167 LFNGVTLFVIAGFIIWEAWQRFFEPPAVASGTMMXXXXXXXXXXXXXXWSLMRKSDAKDN 226 LFNGVTLFVIAGFIIWEA +RF+ PP VASG+MM W+LMRK D K+N Sbjct: 104 LFNGVTLFVIAGFIIWEAIERFYNPPTVASGSMMIIASIGLFANLLSAWALMRKGDVKNN 163 Query: 227 INIRSAYLHIMXXXXXXXXXXXXXXIMNLFSWYVADPIISVLVALLILRGAWGVLKQAFH 286 +N+RSAYLH++ +M +F WYVADPIIS+LVALLIL+GAW V+K H Sbjct: 164 VNLRSAYLHVIGDALGSVGAIIAGIVMWIFDWYVADPIISILVALLILKGAWRVIKHTVH 223 Query: 287 ILMEGTPVSVNTNDIKAALLSIDGVEGVHDLHVWTITSGLDALSGHLLIKDHADPHSILQ 346 ILMEGTP++++ N++K AL SI+GV VHDLH+WTITSGLD+LS H+LI+DH D +LQ Sbjct: 224 ILMEGTPITIDQNEVKKALESIEGVVDVHDLHIWTITSGLDSLSCHILIEDHQDSQKVLQ 283 Query: 347 QAINVVEDNFAIQHTTLQIENSSLKHGELR 376 AI+++E+ F I HTT+QIE S + HGE++ Sbjct: 284 DAIHMIEEKFKILHTTIQIETSQIHHGEMK 313 >A4IMT3.1 A4IMT3_GEOTN Cation efflux family protein Length = 308 Score = 318 bits (814), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 161/271 (59%), Positives = 194/271 (71%) Query: 107 MFLEFFGGLVTGSLALLSDSGHMLSDIASLALSLVAMIFAVKPPSLKNSYGYYRFEIMAA 166 M LEF GGLVT SLALLSDSGHMLSD ASL LSLVA+ A KP S K +YG YRFEI+AA Sbjct: 38 MVLEFVGGLVTNSLALLSDSGHMLSDAASLLLSLVAVWLAAKPASPKKTYGLYRFEILAA 97 Query: 167 LFNGVTLFVIAGFIIWEAWQRFFEPPAVASGTMMXXXXXXXXXXXXXXWSLMRKSDAKDN 226 L NGVTL VIA +IIWEA RF PP VASG MM W LMRK+D K N Sbjct: 98 LVNGVTLVVIAAWIIWEAVGRFVNPPDVASGPMMAVAAVGLLANLVSAWVLMRKADVKGN 157 Query: 227 INIRSAYLHIMXXXXXXXXXXXXXXIMNLFSWYVADPIISVLVALLILRGAWGVLKQAFH 286 +N+RSAYLH++ IM LF WY+ADP+ISVLVA+LIL+GA+ V++Q H Sbjct: 158 VNVRSAYLHVLGDALGSVGAIGAGLIMWLFEWYLADPLISVLVAILILKGAFAVVRQTVH 217 Query: 287 ILMEGTPVSVNTNDIKAALLSIDGVEGVHDLHVWTITSGLDALSGHLLIKDHADPHSILQ 346 ILMEGTPV+++ ++K AL I+GV VHDLH+WTITSGLD+LS HLLI+D D +ILQ Sbjct: 218 ILMEGTPVAIDQTEVKTALSGIEGVLDVHDLHIWTITSGLDSLSCHLLIEDGRDAQAILQ 277 Query: 347 QAINVVEDNFAIQHTTLQIENSSLKHGELRV 377 QAI++VE F I+H T+QIE ++HGE+ V Sbjct: 278 QAIDLVETRFHIRHATIQIEMPHIRHGEMEV 308 >B4BKM3.1 B4BKM3_9BACI Cation diffusion facilitator family transporter Length = 308 Score = 318 bits (814), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 161/271 (59%), Positives = 194/271 (71%) Query: 107 MFLEFFGGLVTGSLALLSDSGHMLSDIASLALSLVAMIFAVKPPSLKNSYGYYRFEIMAA 166 M LEF GGLVT SLALLSDSGHMLSD ASL LSLVA+ A KP S K +YG YRFEI+AA Sbjct: 38 MVLEFVGGLVTNSLALLSDSGHMLSDAASLLLSLVAVWLAAKPASPKKTYGLYRFEILAA 97 Query: 167 LFNGVTLFVIAGFIIWEAWQRFFEPPAVASGTMMXXXXXXXXXXXXXXWSLMRKSDAKDN 226 L NGVTL VIA +IIWEA RF PP VASG MM W LMRK+D K N Sbjct: 98 LVNGVTLVVIAAWIIWEAVGRFVNPPDVASGPMMAVAAVGLLANLVSAWVLMRKADVKGN 157 Query: 227 INIRSAYLHIMXXXXXXXXXXXXXXIMNLFSWYVADPIISVLVALLILRGAWGVLKQAFH 286 +N+RSAYLH++ IM LF WY+ADP+ISVLVA+LIL+GA+ V++Q H Sbjct: 158 VNVRSAYLHVLGDALGSVGAIGAGLIMWLFEWYLADPLISVLVAILILKGAFAVVRQTVH 217 Query: 287 ILMEGTPVSVNTNDIKAALLSIDGVEGVHDLHVWTITSGLDALSGHLLIKDHADPHSILQ 346 ILMEGTPV+++ ++K AL I+GV VHDLH+WTITSGLD+LS HLLI+D D +ILQ Sbjct: 218 ILMEGTPVAIDQTEVKTALSGIEGVLDVHDLHIWTITSGLDSLSCHLLIEDGRDAQAILQ 277 Query: 347 QAINVVEDNFAIQHTTLQIENSSLKHGELRV 377 QAI++VE F I+H T+QIE ++HGE+ V Sbjct: 278 QAIDLVETRFHIRHATIQIEMPHIRHGEMEV 308 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,612,658,310 Number of extensions: 50595686 Number of successful extensions: 173869 Number of sequences better than 1.0e-05: 1744 Number of HSP's gapped: 171672 Number of HSP's successfully gapped: 2031 Length of query: 377 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 240 Effective length of database: 1,770,017,148 Effective search space: 424804115520 Effective search space used: 424804115520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 132 (55.5 bits)