BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0016.p (310 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2ZEU5.1 C2ZEU5_BACCE L-lactate dehydrogenase 3 397 e-109 C2ZWE2.1 C2ZWE2_BACCE L-lactate dehydrogenase 3 396 e-108 C2T817.1 C2T817_BACCE L-lactate dehydrogenase 3 395 e-108 C2YHG9.1 C2YHG9_BACCE L-lactate dehydrogenase 3 395 e-108 C2R0C7.1 C2R0C7_BACCE L-lactate dehydrogenase 3 395 e-108 >C2ZEU5.1 C2ZEU5_BACCE L-lactate dehydrogenase 3 Length = 344 Score = 397 bits (1020), Expect = e-109, Method: Compositional matrix adjust. Identities = 172/265 (64%), Positives = 229/265 (86%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 +AI+G+G+VGSSCAYS+VNQ IC+E+++ID ++RA+ +A+DLSHC++FT TRTKVYAG+ Sbjct: 36 IAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGS 95 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC MD+VI+TAG PKPGQSRLD L A+A I + +V +M GFDGIF+IA+NPVDI Sbjct: 96 YEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVNGVMESGFDGIFLIASNPVDI 155 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY VWK+SGLPR+RV+GTGTS+DSSRL+T+LSE+ +DPRS++GY+LGEHG+SQ VAWS Sbjct: 156 ITYQVWKLSGLPRNRVLGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWS 215 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 HVT+GGKPILQI+E+ +ERF +DL++I KT AGWEI+ RKG+T++GIG++LA+I RS Sbjct: 216 HVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIARS 275 Query: 241 ILNDEHKIIAVSAILDGEYGQSGVC 265 I ND+H++IAVSAILDGEYG+ +C Sbjct: 276 IFNDDHRVIAVSAILDGEYGEYDIC 300 >C2ZWE2.1 C2ZWE2_BACCE L-lactate dehydrogenase 3 Length = 316 Score = 396 bits (1018), Expect = e-108, Method: Compositional matrix adjust. Identities = 172/265 (64%), Positives = 229/265 (86%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 +AI+G+G+VGSSCAYS+VNQ IC+E+++ID ++RA+ +A+DLSHC++FT TRTKVYAG+ Sbjct: 8 IAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGS 67 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC MD+VI+TAG PKPGQSRLD L A+A I + +V +M GFDGIF+IA+NPVDI Sbjct: 68 YEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVNGVMESGFDGIFLIASNPVDI 127 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY VWK+SGLPR+RV+GTGTS+DSSRL+T+LSE+ +DPRS++GY+LGEHG+SQ VAWS Sbjct: 128 ITYQVWKLSGLPRNRVLGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWS 187 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 HVT+GGKPILQI+E+ +ERF +DL++I KT AGWEI+ RKG+T++GIG++LA+I RS Sbjct: 188 HVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIARS 247 Query: 241 ILNDEHKIIAVSAILDGEYGQSGVC 265 I ND+H++IAVSAILDGEYG+ +C Sbjct: 248 IFNDDHRVIAVSAILDGEYGEYDIC 272 >C2T817.1 C2T817_BACCE L-lactate dehydrogenase 3 Length = 367 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 172/265 (64%), Positives = 229/265 (86%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 +AI+G+G+VGSSCAYS+VNQ IC+E+++ID ++RA+ +A+DLSHC++FT TRTKVYAG+ Sbjct: 59 IAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGS 118 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC MD+VI+TAG PKPGQSRLD L A+A I + +V +MA GFDGIF++A+NPVDI Sbjct: 119 YEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMASGFDGIFLLASNPVDI 178 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY VWK+SGLPR+RVIGTGTS+DSSRL+T+LSE+ +DPRS++GY+LGEHG+SQ VAWS Sbjct: 179 ITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWS 238 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 HVT+GGKPILQI+E+ +ERF +DL++I KT AGWEI+ RKG+T++GIG++LA+I S Sbjct: 239 HVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIASS 298 Query: 241 ILNDEHKIIAVSAILDGEYGQSGVC 265 I ND+H++IAVSAILDGEYG+ +C Sbjct: 299 IFNDDHRVIAVSAILDGEYGEYDIC 323 >C2YHG9.1 C2YHG9_BACCE L-lactate dehydrogenase 3 Length = 367 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 172/265 (64%), Positives = 229/265 (86%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 +AI+G+G+VGSSCAYS+VNQ IC+E+++ID ++RA+ +A+DLSHC++FT TRTKVYAG+ Sbjct: 59 IAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGS 118 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC MD+VI+TAG PKPGQSRLD L A+A I + +V +MA GFDGIF++A+NPVDI Sbjct: 119 YEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMASGFDGIFLLASNPVDI 178 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY VWK+SGLPR+RVIGTGTS+DSSRL+T+LSE+ +DPRS++GY+LGEHG+SQ VAWS Sbjct: 179 ITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWS 238 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 HVT+GGKPILQI+E+ +ERF +DL++I KT AGWEI+ RKG+T++GIG++LA+I S Sbjct: 239 HVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIASS 298 Query: 241 ILNDEHKIIAVSAILDGEYGQSGVC 265 I ND+H++IAVSAILDGEYG+ +C Sbjct: 299 IFNDDHRVIAVSAILDGEYGEYDIC 323 >C2R0C7.1 C2R0C7_BACCE L-lactate dehydrogenase 3 Length = 344 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 172/265 (64%), Positives = 228/265 (86%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 +AI+G+G+VGSSCAYS+VNQ IC+E+++ID Y+RA+ +A+DLSHC++FT TRTKVYAG+ Sbjct: 36 IAIIGTGLVGSSCAYSIVNQGICEELLLIDINYERAVGEAMDLSHCINFTNTRTKVYAGS 95 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC MD+VI+TAG PKPGQSRLD L A+A I + +V +M GFDGIF++A+NPVDI Sbjct: 96 YEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDI 155 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY VWK+SGLPR+RVIGTGTS+DSSRL+T+LSE+ +DPRS++GY+LGEHG+SQ VAWS Sbjct: 156 ITYEVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWS 215 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 HVT+GGKPILQI+E+ +ERF +DL++I KT AGWEI+ RKG+T++GIG++LA+I S Sbjct: 216 HVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIANS 275 Query: 241 ILNDEHKIIAVSAILDGEYGQSGVC 265 I ND+H++IAVSAILDGEYG+ +C Sbjct: 276 IFNDDHRVIAVSAILDGEYGEYDIC 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,620,516,065 Number of extensions: 62820092 Number of successful extensions: 156513 Number of sequences better than 1.0e-05: 2613 Number of HSP's gapped: 155233 Number of HSP's successfully gapped: 2613 Length of query: 310 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 175 Effective length of database: 1,788,859,178 Effective search space: 313050356150 Effective search space used: 313050356150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)