BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0004.p (155 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q81CL6.1 Q81CL6_BACCR Fosfomycin resistance protein 241 1e-62 C2T1W4.1 C2T1W4_BACCE Fosfomycin resistance protein 241 1e-62 Q736Y5.1 Q736Y5_BACC1 Fosfomycin resistance protein (Glutathione... 241 2e-62 C3I1L1.1 C3I1L1_BACTU Fosfomycin resistance protein 239 4e-62 C3E4D7.1 C3E4D7_BACTU Fosfomycin resistance protein 238 1e-61 >Q81CL6.1 Q81CL6_BACCR Fosfomycin resistance protein Length = 155 Score = 241 bits (616), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 111/153 (72%), Positives = 130/153 (84%) Query: 1 MIKGLYEAHLPVSNLEVSIEFYKKLGLKFAWRDEETAFFWITEGKSWVGLWEGREVQTPY 60 MIKG+YEAHLPVSNL+ SIEFY+KLGLK AW DE TAFFWI EGKSW+GLW G E +T Y Sbjct: 1 MIKGIYEAHLPVSNLQQSIEFYEKLGLKLAWSDESTAFFWIEEGKSWLGLWAGSEYETKY 60 Query: 61 HPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDGN 120 HPSLRHIAF+V+YEDLK SL WL S+QI+AVPFG+ S+EPF+RPHQGNASVYF DPDGN Sbjct: 61 HPSLRHIAFRVSYEDLKESLHWLHSLQIKAVPFGSNESIEPFVRPHQGNASVYFIDPDGN 120 Query: 121 SLELMCFVEVPEELRTVTDKLSFEEWETLTGRS 153 SLEL+C + VP L+ +TDKLSF++WETL + Sbjct: 121 SLELICHIVVPNALKHITDKLSFKDWETLLADT 153 >C2T1W4.1 C2T1W4_BACCE Fosfomycin resistance protein Length = 155 Score = 241 bits (616), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 111/153 (72%), Positives = 130/153 (84%) Query: 1 MIKGLYEAHLPVSNLEVSIEFYKKLGLKFAWRDEETAFFWITEGKSWVGLWEGREVQTPY 60 MIKG+YEAHLPVSNL+ SIEFY+KLGLK AW DE TAFFWI EGKSW+GLW G E +T Y Sbjct: 1 MIKGIYEAHLPVSNLQQSIEFYEKLGLKLAWSDESTAFFWIEEGKSWLGLWAGSEYETKY 60 Query: 61 HPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDGN 120 HPSLRHIAF+V+YEDLK SL WL S+QI+AVPFG+ S+EPF+RPHQGNASVYF DPDGN Sbjct: 61 HPSLRHIAFRVSYEDLKESLHWLHSLQIKAVPFGSNESIEPFVRPHQGNASVYFIDPDGN 120 Query: 121 SLELMCFVEVPEELRTVTDKLSFEEWETLTGRS 153 SLEL+C + VP L+ +TDKLSF++WETL + Sbjct: 121 SLELICHIVVPNALKHITDKLSFKDWETLLADT 153 >Q736Y5.1 Q736Y5_BACC1 Fosfomycin resistance protein (Glutathione transferase) BH1778 EC=2.5.1.18 Length = 155 Score = 241 bits (614), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 111/153 (72%), Positives = 130/153 (84%) Query: 1 MIKGLYEAHLPVSNLEVSIEFYKKLGLKFAWRDEETAFFWITEGKSWVGLWEGREVQTPY 60 MIKG+YEAHLPVSNL+ SIEFY+KLGLK AW DE TAFFWI EGKSW+GLW G E +T Y Sbjct: 1 MIKGIYEAHLPVSNLQKSIEFYEKLGLKLAWSDESTAFFWIEEGKSWLGLWAGPEYETKY 60 Query: 61 HPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDGN 120 HPSLRHIAF+V+YEDLK SL WL S+QI+AVPFG+ S+EPF+RPHQGNASVYF DPDGN Sbjct: 61 HPSLRHIAFRVSYEDLKESLNWLHSLQIKAVPFGSNESIEPFVRPHQGNASVYFIDPDGN 120 Query: 121 SLELMCFVEVPEELRTVTDKLSFEEWETLTGRS 153 SLEL+C + VP L+ +TDKLSF++WETL + Sbjct: 121 SLELICHIVVPNALKHITDKLSFKDWETLLADT 153 >C3I1L1.1 C3I1L1_BACTU Fosfomycin resistance protein Length = 155 Score = 239 bits (611), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 110/153 (71%), Positives = 128/153 (83%) Query: 1 MIKGLYEAHLPVSNLEVSIEFYKKLGLKFAWRDEETAFFWITEGKSWVGLWEGREVQTPY 60 MIKG+YEAHLPVSNL+ SIEFY+KLGLK AW DE TAFFWI EGKSW+GLW G E +T Y Sbjct: 1 MIKGIYEAHLPVSNLQQSIEFYEKLGLKLAWSDESTAFFWIEEGKSWLGLWAGSEYETKY 60 Query: 61 HPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDGN 120 HPSLRHIAF+V+YEDLK SL WL S+QI+AVPFG+ S+EPF+RPHQGNASVYF DPDGN Sbjct: 61 HPSLRHIAFRVSYEDLKESLHWLHSLQIKAVPFGSNESIEPFVRPHQGNASVYFIDPDGN 120 Query: 121 SLELMCFVEVPEELRTVTDKLSFEEWETLTGRS 153 SLEL+C + VP L+ +TD LSF+ WETL + Sbjct: 121 SLELICHIVVPNALKHITDNLSFKNWETLLAET 153 >C3E4D7.1 C3E4D7_BACTU Fosfomycin resistance protein Length = 155 Score = 238 bits (608), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 109/153 (71%), Positives = 129/153 (84%) Query: 1 MIKGLYEAHLPVSNLEVSIEFYKKLGLKFAWRDEETAFFWITEGKSWVGLWEGREVQTPY 60 MIKG+YEAHLPVSNL+ SIEFY+KLGLK AW DE TAFFWI +GKSW+GLW G E +T Y Sbjct: 1 MIKGIYEAHLPVSNLQQSIEFYEKLGLKLAWSDESTAFFWIEDGKSWLGLWAGSEYETKY 60 Query: 61 HPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDGN 120 HPSLRHIAF+V+YEDLK SL WL S+QI+AVPFG+ S+EPF+RPHQGNASVYF DPDGN Sbjct: 61 HPSLRHIAFRVSYEDLKESLHWLHSLQIKAVPFGSNESIEPFVRPHQGNASVYFIDPDGN 120 Query: 121 SLELMCFVEVPEELRTVTDKLSFEEWETLTGRS 153 SLEL+C + VP L+ + DKLSF++WETL + Sbjct: 121 SLELICHIFVPNALKHINDKLSFKDWETLLADT 153 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,019,200,488 Number of extensions: 40273212 Number of successful extensions: 84960 Number of sequences better than 1.0e-05: 175 Number of HSP's gapped: 85088 Number of HSP's successfully gapped: 175 Length of query: 155 Length of database: 3,060,696,203 Length adjustment: 116 Effective length of query: 39 Effective length of database: 1,967,858,463 Effective search space: 76746480057 Effective search space used: 76746480057 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)