BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0001.p (210 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4K5.1 B3K4K5_9BACI NAD-dependent epimerase/dehydratase 263 6e-69 B2T356.1 B2T356_BURPP NAD-dependent epimerase/dehydratase 187 4e-46 B1GAI7.1 B1GAI7_9BURK NAD-dependent epimerase/dehydratase 186 1e-45 Q140X8.1 Q140X8_BURXL Putative NADH-flavin reductase protein 179 1e-43 Q63VH2.1 Q63VH2_BURPS Putative exported protein 169 2e-40 >B3K4K5.1 B3K4K5_9BACI NAD-dependent epimerase/dehydratase Length = 210 Score = 263 bits (673), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 124/210 (59%), Positives = 164/210 (78%) Query: 1 MDVVVFGATGTIGRAIVQEALKRKHEVTAAVRNPASLELEHERLHVTTADILDPATVAEA 60 M V + GA+GTIG+ I +EAL+R HEVTA +RNP LE EHERL AD++DP+++ EA Sbjct: 1 MKVALIGASGTIGKRITEEALRRGHEVTAVLRNPDRLEAEHERLQKVKADVMDPSSLEEA 60 Query: 61 VRGHEEVISAFGPAAGRENDLLRAAESLLEGMQAAGLERLLVIGGAGTLKTESGEQLMDT 120 V+GH+ VISAFGP G+E +L+ AA +++EG + G+ RLL++GGAG+L +SG LM+T Sbjct: 61 VQGHDAVISAFGPKFGQEEELVAAARTIVEGTRRGGVTRLLIVGGAGSLIADSGVPLMET 120 Query: 121 PGFPEKFRPLAEAHGHAYEIYSASSLDYTYLSPPAVIRPGRRTGQFRIGLDRLVVDENGD 180 P FPE+ RPLA AH AY++Y S L++TY+SP A+I PG+RTGQFRIG+DRLV DE+G Sbjct: 121 PEFPEEIRPLARAHADAYDLYKESDLEWTYISPAAIIEPGKRTGQFRIGMDRLVTDESGS 180 Query: 181 SSISVEDFAVAVIDELEEGNFSRTRFTVGY 210 S ISVEDFAVAVIDEL++ F ++RFTV Y Sbjct: 181 SRISVEDFAVAVIDELDDPQFIQSRFTVAY 210 >B2T356.1 B2T356_BURPP NAD-dependent epimerase/dehydratase Length = 215 Score = 187 bits (476), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 102/211 (48%), Positives = 143/211 (67%), Gaps = 1/211 (0%) Query: 1 MDVVVFGATGTIGRAIVQEALKRKHEVTAAVRNPASLELEHERLHVTTADILDPATVAEA 60 + + +FGATGTIG I EA +R H+VTA VRNPA + + L AD+LD A+V A Sbjct: 5 LKIALFGATGTIGSRIAAEAARRGHQVTALVRNPARVPTDVANLKAAQADLLDAASVGAA 64 Query: 61 VRGHEEVISAFGPAAGRENDLLRAAESLLEGMQAAGLERLLVIGGAGTLKTESGEQLMDT 120 VRGH+ V SA+ P L +A +L+EG++AAGL+RL+V+GGAG+L+ G+QL+D+ Sbjct: 65 VRGHDVVASAYAPPQDDLAALDKATHALIEGVRAAGLKRLVVVGGAGSLEVAPGKQLVDS 124 Query: 121 PGFPEKFRPLAEAHGHAYEIY-SASSLDYTYLSPPAVIRPGRRTGQFRIGLDRLVVDENG 179 GFP+ ++ +A AH A + Y A+ LD+T+ +P A+I PG RTG+FR G + L+ D G Sbjct: 125 EGFPDAYKAIALAHRKALDDYRRAADLDWTFFAPAAIIAPGERTGKFRTGANTLLADAEG 184 Query: 180 DSSISVEDFAVAVIDELEEGNFSRTRFTVGY 210 +S IS ED+AVA +DELE+G F R TV Y Sbjct: 185 NSRISAEDYAVAFVDELEQGRFIRQIATVAY 215 >B1GAI7.1 B1GAI7_9BURK NAD-dependent epimerase/dehydratase Length = 215 Score = 186 bits (472), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 101/211 (47%), Positives = 140/211 (66%), Gaps = 1/211 (0%) Query: 1 MDVVVFGATGTIGRAIVQEALKRKHEVTAAVRNPASLELEHERLHVTTADILDPATVAEA 60 + + +FGATG IG I EA +R H+VTA RNPA + + L AD+LD A+V A Sbjct: 5 LKIALFGATGMIGSRIAAEAARRGHQVTALARNPARVPADVPNLKAAQADLLDAASVGAA 64 Query: 61 VRGHEEVISAFGPAAGRENDLLRAAESLLEGMQAAGLERLLVIGGAGTLKTESGEQLMDT 120 VRGH+ V SA+ P G L +A +L+EG++AAGL+RL+V+GGAG+L+ G+QL+D+ Sbjct: 65 VRGHDVVASAYAPPQGDVGVLDKATRALVEGVRAAGLKRLVVVGGAGSLEVAPGKQLVDS 124 Query: 121 PGFPEKFRPLAEAHGHAYEIY-SASSLDYTYLSPPAVIRPGRRTGQFRIGLDRLVVDENG 179 GFP+ ++ +A AH A Y S + LD+T+ SP A+I PG RTG FR G + L+ D G Sbjct: 125 EGFPDAYKAVALAHRDALGYYRSVADLDWTFFSPAAIIAPGERTGTFRTGTNSLIADAEG 184 Query: 180 DSSISVEDFAVAVIDELEEGNFSRTRFTVGY 210 +S IS ED+A+A +DELE+G F R TV Y Sbjct: 185 NSRISAEDYAIAFVDELEQGRFIRQLATVAY 215 >Q140X8.1 Q140X8_BURXL Putative NADH-flavin reductase protein Length = 215 Score = 179 bits (455), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 97/211 (45%), Positives = 138/211 (65%), Gaps = 1/211 (0%) Query: 1 MDVVVFGATGTIGRAIVQEALKRKHEVTAAVRNPASLELEHERLHVTTADILDPATVAEA 60 + + +FGATG IG + EA +R H+VTA RNPA + L AD+LD A+V A Sbjct: 5 LKIALFGATGMIGSRVAAEAARRGHQVTALARNPARVPAGVANLKAAQADLLDAASVGAA 64 Query: 61 VRGHEEVISAFGPAAGRENDLLRAAESLLEGMQAAGLERLLVIGGAGTLKTESGEQLMDT 120 VRGH+ V SA+ P L +A +L+EG++AAGL+RL+V+GGAG+L+ G+QL+D Sbjct: 65 VRGHDVVASAYAPPQDDLAALGKATRALVEGVRAAGLKRLVVVGGAGSLEVAPGKQLVDA 124 Query: 121 PGFPEKFRPLAEAHGHAYEIY-SASSLDYTYLSPPAVIRPGRRTGQFRIGLDRLVVDENG 179 GFP+ ++ +A AH A+ Y + LD+T+ +P A+I PG RTG+FR G + L+ D G Sbjct: 125 EGFPDAYKAIALAHRDAFAYYRGVTDLDWTFFAPAALIAPGERTGKFRTGANTLLTDAEG 184 Query: 180 DSSISVEDFAVAVIDELEEGNFSRTRFTVGY 210 +S IS EDFA+A +DELE+G+F R T Y Sbjct: 185 NSRISAEDFAIAFVDELEQGHFVRQIATAAY 215 >Q63VH2.1 Q63VH2_BURPS Putative exported protein Length = 208 Score = 169 bits (428), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 96/211 (45%), Positives = 139/211 (65%), Gaps = 4/211 (1%) Query: 1 MDVVVFGATGTIGRAIVQEALKRKHEVTAAVRNPASLELEHERLHVTTADILDPATVAEA 60 M + +FGATG IG I EA +R H+VTA RNPA+ + AD+ DPA++A A Sbjct: 1 MKIALFGATGMIGSRIAAEAARRGHQVTALSRNPAA---SGANVQAKAADLFDPASIAAA 57 Query: 61 VRGHEEVISAFGPAAGRENDLLRAAESLLEGMQAAGLERLLVIGGAGTLKTESGEQLMDT 120 + G + V SA+GP + ++ A++L++G + AG++R++V+GGAGTL+ G+QL+DT Sbjct: 58 LAGQDVVASAYGPKQEEASKVVAVAKALVDGARKAGVKRVVVVGGAGTLEVAPGKQLVDT 117 Query: 121 PGFPEKFRPLAEAHGHAYEIYSA-SSLDYTYLSPPAVIRPGRRTGQFRIGLDRLVVDENG 179 GFP+ ++ +A AH AY S LD+T+ SP A+I PG RTG+FR G RL+VDE G Sbjct: 118 EGFPDAYKAVALAHRDAYGYLSTVQDLDWTFFSPAALIAPGERTGRFRTGAGRLIVDEQG 177 Query: 180 DSSISVEDFAVAVIDELEEGNFSRTRFTVGY 210 +S IS ED+A+A +DE+E+G F R T Y Sbjct: 178 NSKISAEDYAIAFVDEIEQGRFIRQAATAAY 208 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,229,165,694 Number of extensions: 49744566 Number of successful extensions: 168617 Number of sequences better than 1.0e-05: 698 Number of HSP's gapped: 167981 Number of HSP's successfully gapped: 698 Length of query: 210 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 80 Effective length of database: 1,835,964,253 Effective search space: 146877140240 Effective search space used: 146877140240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)