BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0020.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DM81.1 B1DM81_9BACL Methylenetetrahydrofolate dehydrogenase (N... 422 e-116 C0ZC05.1 FOLD_BREBN Bifunctional protein folD Methylenetetrahydr... 378 e-103 C0GE28.1 C0GE28_9FIRM Methylenetetrahydrofolate dehydrogenase (N... 374 e-102 B4AL60.1 B4AL60_BACPU Bifunctional protein FolD EC=1.5.1.5 EC=3.... 373 e-101 A8FF15.1 FOLD_BACP2 Bifunctional protein folD Methylenetetrahydr... 372 e-101 >B1DM81.1 B1DM81_9BACL Methylenetetrahydrofolate dehydrogenase (NADP(+)) EC=1.5.1.5 Length = 287 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 206/280 (73%), Positives = 244/280 (87%) Query: 1 MTAAIISGKQVSDEIRTGIAREVAQLAERGVKPGLAVVLVGEDPASQVYVRNKEKSSTEL 60 M+A II+GK++SD IR I +E A L E+GV PGLAVVLVGEDPAS+VYV +KEK+ +L Sbjct: 2 MSAQIIAGKKISDMIREEIQKETADLREKGVVPGLAVVLVGEDPASKVYVGSKEKACQQL 61 Query: 61 GFHSEVHRLDAATSQEDLLALVAELNRRDEIDGILVQLPLPKHIDEKAVINAIAVEKDVD 120 GF+SEVHRL+ + S+E LLAL+ +LN D+I+GILVQLPLPKHI+EKAVI+AI V+KDVD Sbjct: 62 GFYSEVHRLEESASEEQLLALIDQLNNDDKINGILVQLPLPKHINEKAVIDAIRVDKDVD 121 Query: 121 GFHPINVGNLVIGDDSLLPCTPAGVIELIKRTGTGMSGKHAVVIGRSNIVGKPVSLLLQR 180 GFHP +VGNLVIGDDSLLPCTPAG IELIKR+G +SGKHAVVIGRSNIVGKPV++LL R Sbjct: 122 GFHPESVGNLVIGDDSLLPCTPAGCIELIKRSGVDISGKHAVVIGRSNIVGKPVAMLLLR 181 Query: 181 ENATVTMCHSRTANIAELSRQADILVVAIGRANFIDGSFVKPGAVVIDVGMNRLDNGKLA 240 ENATVT+CHSRTAN+ E+++QADILVVAIG+A ID +VKPGAVVIDVG+NRL +GKL Sbjct: 182 ENATVTICHSRTANMEEIAKQADILVVAIGKAKAIDSKYVKPGAVVIDVGINRLPDGKLC 241 Query: 241 GDVDYESAKEVAGYITPVPGGVGPMTITMLMANTLTAAKR 280 GDVDY+ E AG+ITPVPGGVGPMTITMLM NT+TAAKR Sbjct: 242 GDVDYDDCLETAGFITPVPGGVGPMTITMLMNNTITAAKR 281 >C0ZC05.1 FOLD_BREBN Bifunctional protein folD Methylenetetrahydrofolate dehydrogenase Methenyltetrahydrofolate cyclohydrolase EC=1.5.1.5 EC=3.5.4.9 Length = 284 Score = 378 bits (970), Expect = e-103, Method: Compositional matrix adjust. Identities = 181/281 (64%), Positives = 226/281 (80%) Query: 1 MTAAIISGKQVSDEIRTGIAREVAQLAERGVKPGLAVVLVGEDPASQVYVRNKEKSSTEL 60 M A I+ GK+V+ IR +A EV +L ++GV PGL VV+VG+DPAS YVR K K E+ Sbjct: 1 MAATILQGKEVAQSIRAELANEVVELKKQGVVPGLTVVIVGDDPASHSYVRGKAKGCEEV 60 Query: 61 GFHSEVHRLDAATSQEDLLALVAELNRRDEIDGILVQLPLPKHIDEKAVINAIAVEKDVD 120 G SE+ R DA ++E+LL ++ +LN ++GILVQLPLP HI E AVI AIA EKDVD Sbjct: 61 GISSEIIRKDADITEEELLVIIQQLNENPNVNGILVQLPLPAHISEHAVIEAIAPEKDVD 120 Query: 121 GFHPINVGNLVIGDDSLLPCTPAGVIELIKRTGTGMSGKHAVVIGRSNIVGKPVSLLLQR 180 GFHPI++GN+V+G+D++LPCTP G+IELIKRTGT M+GKHAVVIGRSNIVGKPVSLLLQ+ Sbjct: 121 GFHPISIGNMVLGNDTMLPCTPHGIIELIKRTGTQMAGKHAVVIGRSNIVGKPVSLLLQQ 180 Query: 181 ENATVTMCHSRTANIAELSRQADILVVAIGRANFIDGSFVKPGAVVIDVGMNRLDNGKLA 240 ENATVTMCHSRT N+ E +++ADILVVA GRA+ I VKPGAVVIDVG+NR++ GKL Sbjct: 181 ENATVTMCHSRTQNLEEYTQKADILVVATGRAHMIGKEHVKPGAVVIDVGVNRIETGKLV 240 Query: 241 GDVDYESAKEVAGYITPVPGGVGPMTITMLMANTLTAAKRR 281 GDV ++ K+VA +ITPVPGGVGPMTITML+ NT+ AAK++ Sbjct: 241 GDVKFDEVKDVASFITPVPGGVGPMTITMLLKNTVAAAKKQ 281 >C0GE28.1 C0GE28_9FIRM Methylenetetrahydrofolate dehydrogenase (NADP(+)) EC=1.5.1.5 Length = 284 Score = 374 bits (959), Expect = e-102, Method: Compositional matrix adjust. Identities = 185/285 (64%), Positives = 233/285 (81%), Gaps = 2/285 (0%) Query: 1 MTAAIISGKQVSDEIRTGIAREVAQLAER-GVKPGLAVVLVGEDPASQVYVRNKEKSSTE 59 MTA +ISG +V+ IR I+ E+A L E+ G PGLAV+LVGEDPASQ YV NKEK++ + Sbjct: 1 MTAKLISGPEVAAAIREQISTELASLKEKTGKVPGLAVILVGEDPASQAYVGNKEKTAGK 60 Query: 60 LGFHSEVHRLDAATSQEDLLALVAELNRRDEIDGILVQLPLPKHIDEKAVINAIAVEKDV 119 LGFHS V R+ A +QE+LL + +LN+ ++I GILVQLPLP HI+EKAVI+AIAVEKDV Sbjct: 61 LGFHSVVDRVSADITQEELLKKIDDLNKDEDIHGILVQLPLPDHIEEKAVIDAIAVEKDV 120 Query: 120 DGFHPINVGNLVIGDDSLLPCTPAGVIELIKRTGTGMSGKHAVVIGRSNIVGKPVSLLLQ 179 DGFHPINVGNL+IGDD +PCTP G +++++ G + GK+AVV+GRSNIVGKPV+LL+ Sbjct: 121 DGFHPINVGNLMIGDDCYIPCTPHGCMKMLEHIGYDLKGKNAVVVGRSNIVGKPVALLML 180 Query: 180 RENATVTMCHSRTANIAELSRQADILVVAIGRANFIDGSFVKPGAVVIDVGMNRLDNGKL 239 +++ATVT+CHSRTA++ + R+AD+LVVA+GR N I G VKPGAVVIDVG+NR+D GKL Sbjct: 181 QQHATVTICHSRTADLGAVCREADVLVVAVGRPNMITGDMVKPGAVVIDVGINRVD-GKL 239 Query: 240 AGDVDYESAKEVAGYITPVPGGVGPMTITMLMANTLTAAKRRRGL 284 GDV+++SAKEVA YITPVPGGVGPMTITMLM NTL A KR+ L Sbjct: 240 VGDVEFDSAKEVASYITPVPGGVGPMTITMLMLNTLEAFKRKNNL 284 >B4AL60.1 B4AL60_BACPU Bifunctional protein FolD EC=1.5.1.5 EC=3.5.4.9 Length = 283 Score = 373 bits (957), Expect = e-101, Method: Compositional matrix adjust. Identities = 176/280 (62%), Positives = 222/280 (79%) Query: 1 MTAAIISGKQVSDEIRTGIAREVAQLAERGVKPGLAVVLVGEDPASQVYVRNKEKSSTEL 60 MTA II GK+ + E R +A+EV +L ++GV PGLAV+L+G+DPAS YVR K+K++ + Sbjct: 1 MTATIIDGKETAKEKREQLAKEVEELKQKGVTPGLAVILIGDDPASLSYVRGKKKAAEAM 60 Query: 61 GFHSEVHRLDAATSQEDLLALVAELNRRDEIDGILVQLPLPKHIDEKAVINAIAVEKDVD 120 G H ++ LDA+ ++E LL L+ + N D+ GILVQLPLPKHI EKAVI I+ EKDVD Sbjct: 61 GMHFQLDHLDASLTEEALLQLIDQYNANDQFHGILVQLPLPKHISEKAVIERISPEKDVD 120 Query: 121 GFHPINVGNLVIGDDSLLPCTPAGVIELIKRTGTGMSGKHAVVIGRSNIVGKPVSLLLQR 180 GFHP+N+G +++G+D+ LPCTPAG++EL+ +TG +SGK VV+GRSNIVGKPV LL Sbjct: 121 GFHPLNIGKMLLGEDTFLPCTPAGIVELLNKTGVSLSGKEVVVVGRSNIVGKPVGQLLLN 180 Query: 181 ENATVTMCHSRTANIAELSRQADILVVAIGRANFIDGSFVKPGAVVIDVGMNRLDNGKLA 240 ENATVT CHSRTANI+E + +ADILVVA+GRANFI +K GA+VIDVG+NRLDNGKL Sbjct: 181 ENATVTYCHSRTANISEHTLKADILVVAVGRANFIKADQIKEGAIVIDVGVNRLDNGKLV 240 Query: 241 GDVDYESAKEVAGYITPVPGGVGPMTITMLMANTLTAAKR 280 GDVD+E AKE A YITPVPGGVGPMTITML NT+ +AKR Sbjct: 241 GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKR 280 >A8FF15.1 FOLD_BACP2 Bifunctional protein folD Methylenetetrahydrofolate dehydrogenase Methenyltetrahydrofolate cyclohydrolase EC=1.5.1.5 EC=3.5.4.9 Length = 283 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 175/280 (62%), Positives = 222/280 (79%) Query: 1 MTAAIISGKQVSDEIRTGIAREVAQLAERGVKPGLAVVLVGEDPASQVYVRNKEKSSTEL 60 MTA II GK+ + E R +A+EV +L ++GV PGLAV+L+G+DPAS YVR K+K++ + Sbjct: 1 MTATIIDGKETAKEKREQLAKEVEELKQKGVTPGLAVILIGDDPASLSYVRGKKKAAEAM 60 Query: 61 GFHSEVHRLDAATSQEDLLALVAELNRRDEIDGILVQLPLPKHIDEKAVINAIAVEKDVD 120 G H ++ LDA+ ++E+LL L+ + N D+ GILVQLPLPKHI EKAVI I+ EKDVD Sbjct: 61 GMHFQLDHLDASLTEEELLQLIDQYNANDQFHGILVQLPLPKHISEKAVIERISPEKDVD 120 Query: 121 GFHPINVGNLVIGDDSLLPCTPAGVIELIKRTGTGMSGKHAVVIGRSNIVGKPVSLLLQR 180 GFHP+N+G +++G+D+ LPCTPAG++EL+ +TG +SGK VV+GRSNIVGKPV LL Sbjct: 121 GFHPLNIGKMLLGEDTFLPCTPAGIVELLNKTGVNLSGKEVVVVGRSNIVGKPVGQLLLN 180 Query: 181 ENATVTMCHSRTANIAELSRQADILVVAIGRANFIDGSFVKPGAVVIDVGMNRLDNGKLA 240 ENATVT CHSRTANI+E + +ADILVVA+GRANFI +K GA+VIDVG+NRLD GKL Sbjct: 181 ENATVTYCHSRTANISEHTLKADILVVAVGRANFIKADQIKEGAIVIDVGVNRLDTGKLV 240 Query: 241 GDVDYESAKEVAGYITPVPGGVGPMTITMLMANTLTAAKR 280 GDVD+E AKE A YITPVPGGVGPMTITML NT+ +AKR Sbjct: 241 GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKR 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,584,941,551 Number of extensions: 65749252 Number of successful extensions: 198524 Number of sequences better than 1.0e-05: 1960 Number of HSP's gapped: 195854 Number of HSP's successfully gapped: 1967 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)