BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0009.p (360 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK34.1 B3KK34_9BACI Stage III sporulation protein AE 540 e-152 B1DM91.1 B1DM91_9BACL Stage III sporulation protein AE 476 e-132 C0ZBZ4.1 C0ZBZ4_BREBN Stage III sporulation protein AE 396 e-108 Q9K957.1 Q9K957_BACHD Mutants block sporulation after engulfment 342 5e-92 Q2B5Q8.1 Q2B5Q8_9BACI SpoIIIAE 327 2e-87 >B3KK34.1 B3KK34_9BACI Stage III sporulation protein AE Length = 403 Score = 540 bits (1392), Expect = e-152, Method: Compositional matrix adjust. Identities = 259/360 (71%), Positives = 308/360 (85%), Gaps = 1/360 (0%) Query: 1 VKGQVQNLPTGEVESYWNKLMKDYGGFFPEGKTPSLMDMLLPGEDGLSFKSVLKGLTSFM 60 V+ Q Q LPT +VE YW++LMKDYGGFFP+GKTPSLMDMLLPG DGLSFK V+ G+ ++ Sbjct: 45 VEQQTQQLPTDQVEKYWDRLMKDYGGFFPDGKTPSLMDMLLPGGDGLSFKGVMSGIGKYL 104 Query: 61 WHEVMFNGKLLVTIVMISVLSMILETLQTAFERKSVSKIAYMLCYMVVLVIAVNSFNVAI 120 WHEV++NGKLL TIVM+S++S+ILE LQTAFE+ +VS++AY +CYMVVLVIAVNSF++AI Sbjct: 105 WHEVLYNGKLLATIVMLSIMSLILENLQTAFEKSTVSRVAYTICYMVVLVIAVNSFSIAI 164 Query: 121 GYAKDAIDRMTDFMMAMIPLLFALLASMGNIVTVSVTHPLIVFMIHTVSTLIHTVVFPLF 180 GYA+DAI +M DFMMAM+PLLFAL+ASMGN+VTVSV HPL++FM+HTV TLI T+VFPL Sbjct: 165 GYARDAITQMIDFMMAMLPLLFALMASMGNVVTVSVAHPLVIFMVHTVGTLISTIVFPLL 224 Query: 181 FFSAVLHLVSAMSDKYKLTQXXXXXXXXXXXXXXXXXXXXXXXISVRGITSSVTDGVTLR 240 FFSA+LHLVS++S+KYKLTQ ISV+G+TSSVTDGVT+R Sbjct: 225 FFSALLHLVSSLSEKYKLTQLGNLLRGISMGVLGVLLTVFLGVISVQGLTSSVTDGVTIR 284 Query: 241 AAKYITGNFVPVIGKMFADATDTVISASLLVKNAIGLSGVIIILFLCAFPAIKILVLALI 300 AAKY+TGNFVPV+GKMFADATDTVISASLLVKN+IGL+GVIIILFLCAFPAIKIL LALI Sbjct: 285 AAKYVTGNFVPVVGKMFADATDTVISASLLVKNSIGLAGVIIILFLCAFPAIKILTLALI 344 Query: 301 YNVAGAXXXXPLGETPIVVCLQTIGKSMIYVFAALAAVSLMFFLAVTIMLTAGNVTVMMR 360 YNVA A PLG++PI CLQTIGK+M+YVFAALAAVSLMFFLA+TI+LTA N+TVMMR Sbjct: 345 YNVAAA-VMQPLGDSPIGTCLQTIGKTMLYVFAALAAVSLMFFLAITILLTAANITVMMR 403 >B1DM91.1 B1DM91_9BACL Stage III sporulation protein AE Length = 364 Score = 476 bits (1226), Expect = e-132, Method: Compositional matrix adjust. Identities = 231/359 (64%), Positives = 287/359 (79%), Gaps = 1/359 (0%) Query: 2 KGQVQNLPTGEVESYWNKLMKDYGGFFPEGKTPSLMDMLLPGEDGLSFKSVLKGLTSFMW 61 + Q++ L TG VE+YWN+L +YGG+FP+ K PS +ML PG +GL +V KGL + + Sbjct: 7 ESQLKGLDTGTVENYWNQLKVEYGGYFPDEKMPSFFEMLKPGGEGLKLSTVFKGLLGYFF 66 Query: 62 HEVMFNGKLLVTIVMISVLSMILETLQTAFERKSVSKIAYMLCYMVVLVIAVNSFNVAIG 121 HEV++NGKLLVTIVM++V SM+LETLQ AFER +VSK+AY + YMV+++IAVNSF+VAIG Sbjct: 67 HEVLYNGKLLVTIVMLTVFSMVLETLQNAFERNAVSKVAYSITYMVLIIIAVNSFHVAIG 126 Query: 122 YAKDAIDRMTDFMMAMIPLLFALLASMGNIVTVSVTHPLIVFMIHTVSTLIHTVVFPLFF 181 YAKDAI M FM+AM+PLL LLA+MGN++TVS+ HPLI+FMIHTV T+I+T+VFPL F Sbjct: 127 YAKDAIGTMIQFMLAMMPLLLTLLAAMGNVLTVSILHPLIIFMIHTVGTVIYTIVFPLLF 186 Query: 182 FSAVLHLVSAMSDKYKLTQXXXXXXXXXXXXXXXXXXXXXXXISVRGITSSVTDGVTLRA 241 FSAVLH+VSAMSDK+K+TQ +SV+G +VTDG+T+R Sbjct: 187 FSAVLHIVSAMSDKFKVTQLANLLRNIGVGIMGVLVTVFLGVLSVQGAKGAVTDGITMRT 246 Query: 242 AKYITGNFVPVIGKMFADATDTVISASLLVKNAIGLSGVIIILFLCAFPAIKILVLALIY 301 AK++TGNFVPV+G+MF DA DTVISASLL KNAIGL+GVII+LFLCAFPAIKIL LALIY Sbjct: 247 AKFVTGNFVPVVGRMFTDAADTVISASLLAKNAIGLAGVIILLFLCAFPAIKILTLALIY 306 Query: 302 NVAGAXXXXPLGETPIVVCLQTIGKSMIYVFAALAAVSLMFFLAVTIMLTAGNVTVMMR 360 NV+ A PLG++PIV CLQTIGK+MIYVFAALAAVSLMFFLAVTI+LTAGN +M+R Sbjct: 307 NVSAA-AMQPLGDSPIVACLQTIGKTMIYVFAALAAVSLMFFLAVTIVLTAGNAALMVR 364 >C0ZBZ4.1 C0ZBZ4_BREBN Stage III sporulation protein AE Length = 398 Score = 396 bits (1018), Expect = e-108, Method: Compositional matrix adjust. Identities = 192/360 (53%), Positives = 270/360 (75%), Gaps = 2/360 (0%) Query: 1 VKGQVQNLPTGEVESYWNKLMKDYGGFFPEGKTPSLMDMLLPGEDGLSFKSVLKGLTSFM 60 V+ QV +L VE YW +L++DY G+ P+ K+ + +L+ D S VL+G+ F+ Sbjct: 41 VQQQVDHLQLDRVEQYWKQLLRDYKGYLPDLKSEGFIQILMQQGD-FSISGVLQGMGKFI 99 Query: 61 WHEVMFNGKLLVTIVMISVLSMILETLQTAFERKSVSKIAYMLCYMVVLVIAVNSFNVAI 120 +HE++ NGKLL +I++I+V +MILET+Q AFER +VS +AY + Y+V++V+A+NSF+VAI Sbjct: 100 FHEILMNGKLLSSIIIITVFAMILETMQNAFERNAVSTVAYSITYLVLMVLAINSFHVAI 159 Query: 121 GYAKDAIDRMTDFMMAMIPLLFALLASMGNIVTVSVTHPLIVFMIHTVSTLIHTVVFPLF 180 YAKDAI M+DFM+AMIPL+ ALLAS+GN+ + ++ HPLI+FMI+T +I VVFPL Sbjct: 160 TYAKDAIANMSDFMLAMIPLVIALLASVGNLASATMFHPLIIFMINTSGMMISYVVFPLL 219 Query: 181 FFSAVLHLVSAMSDKYKLTQXXXXXXXXXXXXXXXXXXXXXXXISVRGITSSVTDGVTLR 240 F SA+L +VS S++YK+TQ IS++G TS+V DGVTLR Sbjct: 220 FLSAMLSIVSLFSERYKVTQLATLLRNIAMGVLGSFLTIFLAIISIQGATSAVADGVTLR 279 Query: 241 AAKYITGNFVPVIGKMFADATDTVISASLLVKNAIGLSGVIIILFLCAFPAIKILVLALI 300 AKYITGNF+P++G++F+DA DTV++ASLLVKNA+GL+GV+I++ LCAFPA+KILVLALI Sbjct: 280 TAKYITGNFIPIVGRVFSDAADTVLNASLLVKNAVGLAGVLILIMLCAFPALKILVLALI 339 Query: 301 YNVAGAXXXXPLGETPIVVCLQTIGKSMIYVFAALAAVSLMFFLAVTIMLTAGNVTVMMR 360 YN++ A PLG +PI+ L TIGKS+++VFAALA V LMFFLA+TIM+ AGN+++M+R Sbjct: 340 YNLSSA-VLQPLGNSPIISALGTIGKSLLFVFAALATVGLMFFLAITIMIAAGNISMMVR 398 >Q9K957.1 Q9K957_BACHD Mutants block sporulation after engulfment Length = 397 Score = 342 bits (876), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 174/360 (48%), Positives = 258/360 (71%), Gaps = 2/360 (0%) Query: 1 VKGQVQNLPTGEVESYWNKLMKDYGGFFPEGKTPSLMDMLLPGEDGLSFKSVLKGLTSFM 60 ++ Q+++L E++ +W+++ +YGGF PE + SLM+ L G+ GL ++ Sbjct: 40 IERQIESLEIEEIQQFWHEIATEYGGFLPESQKGSLME-FLRGDKQFRIDEWGMGLVKYL 98 Query: 61 WHEVMFNGKLLVTIVMISVLSMILETLQTAFERKSVSKIAYMLCYMVVLVIAVNSFNVAI 120 HE++ NGKLL +I+++SV S++L++LQ AFE+K++SK+AY + YMV+++IA+NSF+VA+ Sbjct: 99 LHELLVNGKLLGSIILLSVFSVMLQSLQNAFEQKTISKVAYAITYMVLMLIALNSFHVAM 158 Query: 121 GYAKDAIDRMTDFMMAMIPLLFALLASMGNIVTVSVTHPLIVFMIHTVSTLIHTVVFPLF 180 Y K+AI M FM+A++PLL AL+A++GNI + ++ HPLIVFM++T +IH V PL Sbjct: 159 DYTKEAISSMVHFMVALLPLLLALMAAIGNITSAALFHPLIVFMVNTSGMVIHHFVLPLL 218 Query: 181 FFSAVLHLVSAMSDKYKLTQXXXXXXXXXXXXXXXXXXXXXXXISVRGITSSVTDGVTLR 240 F S VL +VS M+D YK+T+ ISV+G T++VTDG+T+R Sbjct: 219 FISTVLSIVSTMTDHYKVTKLANFLRTIAVGVLGVFLTVFLGIISVQGATTAVTDGITVR 278 Query: 241 AAKYITGNFVPVIGKMFADATDTVISASLLVKNAIGLSGVIIILFLCAFPAIKILVLALI 300 AKYI GNFVPV+G+MF DA DTV+SASLL+KN +G++G+ I+L LCAFPAIK+L LA+I Sbjct: 279 TAKYIAGNFVPVVGRMFTDAADTVMSASLLLKNTVGVAGLAILLMLCAFPAIKVLSLAVI 338 Query: 301 YNVAGAXXXXPLGETPIVVCLQTIGKSMIYVFAALAAVSLMFFLAVTIMLTAGNVTVMMR 360 ++++ A PLG PI CL IGK++I++FA+LA V LMFFLA+TIM+ AGN +VM+R Sbjct: 339 FHISAA-ILQPLGAGPITDCLSIIGKAIIFIFASLAVVCLMFFLAITIMIVAGNASVMIR 397 >Q2B5Q8.1 Q2B5Q8_9BACI SpoIIIAE Length = 399 Score = 327 bits (837), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 172/360 (47%), Positives = 251/360 (69%), Gaps = 2/360 (0%) Query: 1 VKGQVQNLPTGEVESYWNKLMKDYGGFFPEGKTPSLMDMLLPGEDGLSFKSVLKGLTSFM 60 V+ Q++ L E++ +W K+ +YGGF PE + SL + + G+ S K + G F+ Sbjct: 42 VEAQLETLELDELKGFWEKVSNEYGGFLPESQKGSLYE-FIKGDKEFSLKEWMLGGLKFL 100 Query: 61 WHEVMFNGKLLVTIVMISVLSMILETLQTAFERKSVSKIAYMLCYMVVLVIAVNSFNVAI 120 +HE + NGKLL ++++++V SM L++LQ +FE+ SVSK AY + +MV+++IA+NSF VA+ Sbjct: 101 FHEFLVNGKLLGSLIILTVFSMFLQSLQNSFEKSSVSKAAYSIIFMVLVIIALNSFRVAV 160 Query: 121 GYAKDAIDRMTDFMMAMIPLLFALLASMGNIVTVSVTHPLIVFMIHTVSTLIHTVVFPLF 180 Y+ +AI MT F++A++PLL AL+AS G +V+ + HP+I+F+++ I V PL Sbjct: 161 DYSIEAISTMTSFILALVPLLLALIASSGGLVSAAFFHPVILFLMNISGLFIQYAVLPLL 220 Query: 181 FFSAVLHLVSAMSDKYKLTQXXXXXXXXXXXXXXXXXXXXXXXISVRGITSSVTDGVTLR 240 F SAVL +VS +S+ YK+TQ ISV+G ++++TDGVTLR Sbjct: 221 FLSAVLSIVSVLSENYKVTQLANLLRNWSIGLLGLFVTIFLGVISVQGASAAITDGVTLR 280 Query: 241 AAKYITGNFVPVIGKMFADATDTVISASLLVKNAIGLSGVIIILFLCAFPAIKILVLALI 300 AK+ITGNF+PVIG+MF DATDTVISAS+L+KN +G++GV I+L + AFPAIKIL++A I Sbjct: 281 TAKFITGNFIPVIGRMFTDATDTVISASVLLKNTVGIAGVAILLIIAAFPAIKILMIAFI 340 Query: 301 YNVAGAXXXXPLGETPIVVCLQTIGKSMIYVFAALAAVSLMFFLAVTIMLTAGNVTVMMR 360 Y A A PLG P++ CL I KS+IYVFAALA VSLMFFL++T+++ AGN+T+MMR Sbjct: 341 YKFAAA-VLQPLGGGPVIACLDIISKSVIYVFAALALVSLMFFLSITVIIAAGNLTMMMR 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.139 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,643,470,422 Number of extensions: 57529400 Number of successful extensions: 223149 Number of sequences better than 1.0e-05: 190 Number of HSP's gapped: 222693 Number of HSP's successfully gapped: 190 Length of query: 360 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 223 Effective length of database: 1,770,017,148 Effective search space: 394713824004 Effective search space used: 394713824004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)