BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0002.p (207 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K511.1 B3K511_9BACI SNARE associated Golgi protein 277 3e-73 C0ZD26.1 C0ZD26_BREBN Conserved hypothetical membrane protein 243 8e-63 A4IJQ1.1 A4IJQ1_GEOTN Alkaline phosphatase-like protein 241 2e-62 Q5L3K5.1 Q5L3K5_GEOKA Alkaline phosphatase 241 3e-62 Q8ENS0.1 Q8ENS0_OCEIH Alkaline phosphatase EC=3.1.3.1 239 9e-62 >B3K511.1 B3K511_9BACI SNARE associated Golgi protein Length = 203 Score = 277 bits (709), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 128/178 (71%), Positives = 157/178 (88%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ WITDIME+FGY GI LM+ALEN+FPPIPSEVIL FGGFMTT + LT+PGV+IA+T G Sbjct: 1 MENWITDIMEKFGYVGILLMIALENVFPPIPSEVILTFGGFMTTYTKLTVPGVIIAATIG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA ILY IGRLL+V R+E+IVDR+G +R+KK DIRKADAWFDKYGYWTVLFCRM+P Sbjct: 61 SVVGAVILYGIGRLLDVERLEKIVDRWGHILRVKKEDIRKADAWFDKYGYWTVLFCRMIP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 L+RSLISIPAGMS MKFG+F+LFTTIGTL WN +L+ +GAA+G+SWE+I ++ YS+ Sbjct: 121 LIRSLISIPAGMSNMKFGMFLLFTTIGTLIWNVILVSVGAAVGDSWENIVAFMDVYSN 178 >C0ZD26.1 C0ZD26_BREBN Conserved hypothetical membrane protein Length = 203 Score = 243 bits (620), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 116/203 (57%), Positives = 147/203 (72%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ W+T I+EQ+ Y GI LM+ALENIFPPIPSEVIL FGGFMTT S LT+ GV++++T G Sbjct: 1 MQNWMTQIIEQYSYFGILLMMALENIFPPIPSEVILTFGGFMTTQSSLTVFGVILSATIG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA ILY IG L+V ++E+IV+R+G +RIK +DI KA+ WFD+YGYWTV FCRMVP Sbjct: 61 SVIGAIILYGIGYYLDVKKIEKIVERWGYLLRIKTADIHKANKWFDRYGYWTVFFCRMVP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSSXX 180 L+RSLISIPAGM+ M F LF+LFTT+GT WN +L++ G LGESWEDI Y YS+ Sbjct: 121 LIRSLISIPAGMTKMNFPLFLLFTTLGTFIWNIILVMAGHLLGESWEDILYYFDLYSNVV 180 Query: 181 XXXXXXXXXXXXXXXXRKRHTEG 203 RKR + Sbjct: 181 YVVLAFAGIVFLILFLRKRKVDA 203 >A4IJQ1.1 A4IJQ1_GEOTN Alkaline phosphatase-like protein Length = 202 Score = 241 bits (616), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 118/178 (66%), Positives = 141/178 (79%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ WIT+ MEQFGY GIF M+ALENIFPPIPSEVILPFGGFMTT + LTIPGV+ A+TAG Sbjct: 1 MQAWITEFMEQFGYIGIFFMIALENIFPPIPSEVILPFGGFMTTYTTLTIPGVIAAATAG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA +LY IGRLL V R+ERIVDR+G W+R+K DI KA+ F +YG W V RM+P Sbjct: 61 SVVGAIVLYGIGRLLSVERLERIVDRWGGWLRVKPEDIAKANRTFQRYGVWAVFLGRMIP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 LVRSLISIPAGMS M GLF+ + +GTL WNTLLI +GAALG+SWE + IG Y+ Sbjct: 121 LVRSLISIPAGMSKMNIGLFVWLSVLGTLIWNTLLISIGAALGQSWEKVSEVIGMYAE 178 >Q5L3K5.1 Q5L3K5_GEOKA Alkaline phosphatase Length = 202 Score = 241 bits (614), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 118/178 (66%), Positives = 140/178 (78%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ WITD MEQFGY GIF M+ALENIFPPIPSEVILPFGGFMTT + LT+PGV+ A+TAG Sbjct: 1 MQAWITDFMEQFGYIGIFFMIALENIFPPIPSEVILPFGGFMTTYTSLTVPGVIAAATAG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S+ GA +LY IGRLL V R+ERIVDR+G W+R+K DI KA+ F +YG W V F RMVP Sbjct: 61 SVAGALVLYGIGRLLSVERLERIVDRWGGWLRVKPEDIAKANLTFHRYGVWAVFFGRMVP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 LVRSLISIPAGM+ M GLF+ + +GTL WNT+LI +GAALGESW + IG Y+ Sbjct: 121 LVRSLISIPAGMAKMNIGLFVWLSVLGTLIWNTILISIGAALGESWGKVSEVIGMYAE 178 >Q8ENS0.1 Q8ENS0_OCEIH Alkaline phosphatase EC=3.1.3.1 Length = 202 Score = 239 bits (611), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 106/177 (59%), Positives = 143/177 (80%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M W+T IMEQ GY GIFLM+ALEN+FPPIPSE++LPFGGF+TT + LTI GV+ A+T G Sbjct: 1 MNDWVTGIMEQLGYLGIFLMMALENLFPPIPSEIVLPFGGFLTTYTNLTILGVITAATLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++G+ +LY IG L++ R+ER++DRYG+WIR+K SD+++A+ WF + GYWTV FCRMVP Sbjct: 61 SVIGSMVLYCIGTFLDIPRLERLIDRYGQWIRLKLSDVQRANDWFQRRGYWTVFFCRMVP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYS 177 ++RSLISIPAGM+ M F +F+ TT+GT+ WN +LI G ALGE+W+ I G++ YS Sbjct: 121 VIRSLISIPAGMTRMNFPIFIFLTTLGTILWNVILISFGVALGENWQQILGFMHVYS 177 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.143 0.455 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,157,048,841 Number of extensions: 44518567 Number of successful extensions: 179696 Number of sequences better than 1.0e-05: 1894 Number of HSP's gapped: 179063 Number of HSP's successfully gapped: 1894 Length of query: 207 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 78 Effective length of database: 1,845,385,268 Effective search space: 143940050904 Effective search space used: 143940050904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)