BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0038.p (461 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WBF4.1 Q5WBF4_BACSK Sugar ABC transporter substrate-binding pr... 208 7e-52 A5UZC1.1 A5UZC1_ROSS1 Extracellular solute-binding protein, fami... 197 2e-48 A7NGQ3.1 A7NGQ3_ROSCS Extracellular solute-binding protein family 1 194 1e-47 C0UUV7.1 C0UUV7_9BACT Carbohydrate ABC transporter substrate-bin... 190 3e-46 C2YQ38.1 C2YQ38_BACCE Extracellular solute-binding protein 124 2e-26 >Q5WBF4.1 Q5WBF4_BACSK Sugar ABC transporter substrate-binding protein Length = 442 Score = 208 bits (530), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 137/392 (34%), Positives = 222/392 (56%), Gaps = 24/392 (6%) Query: 69 KLIPDFEKEHPNIQVKVEKAPYEQFDSKLQTLIAGGKSPDVTS-HYGYGGFAEYYNKDML 127 ++I +FEKEHP I+V+++ P + D KLQT+IAGG PDV S ++ GF Y+ L Sbjct: 52 RVIEEFEKEHPGIKVRLQTIPQGEIDQKLQTMIAGGNVPDVWSPNWADSGFGGYHKLGAL 111 Query: 128 LDLTDLIKEDGFKPEDYH-IPDNLMKIYTVKDRTYGIPVNMYVTLMLYNKDMFDAAKLSY 186 DLT I++D PE I + L++IY ++ T+G+P+ + + YN+D+FDAA + Sbjct: 112 KDLTPYIEQD---PEVVEGIDETLLEIYRTEEGTFGLPILSMGSFLYYNRDLFDAAGIEP 168 Query: 187 PPSDYEDKSWTFDKMVEEAKKMTLVSDDIAKTQ--YGV-DFTWAERDMRPLYFGAEPYSE 243 PP+D+EDKSW +D M+E AK +T +D+ TQ YG+ + RD FG + ++E Sbjct: 169 PPTDWEDKSWDWDAMIEAAKAIT--ENDLPPTQRVYGILNDNSPNRDA--WMFGGDLFAE 224 Query: 244 DTWSNGGVPSETHFDSPKVIAAYHKLFDLIFKDKVSPTSEWSKSVAGQNGDPFVAGKIGM 303 +T+ G + T +P+ A +DLI + +V P ++A Q DPF++G++GM Sbjct: 225 ETYETGELVEPTVTTNPRNYEAIQARYDLINEHEVMPPQSEVAALA-QLSDPFLSGRVGM 283 Query: 304 SIGGSWNLAGANDFPFKIGVAAVPWGGNDKVRSTLFVDPLMILKDSKHPKEAFEWIKYLV 363 + G W + G+AA P+ D+ + L+VDP I + SKHP EA+E++K+L+ Sbjct: 284 VMKGGWGTRQYANTDLDWGLAAYPYTNEDR-QIPLYVDPWSISEGSKHPDEAWEFLKFLM 342 Query: 364 TTEVQEKSIELSGGNPPVNTEAAEAYYKHFE---GIDPQDVKKVYEGAVKYGFESYNHLI 420 + K + P ++ E +Y+ GI D++K EGA+KYG + NHLI Sbjct: 343 DPDKAAKWMVEMTLESPAHSGLKELWYELISERTGISVDDLRKADEGALKYGRPTDNHLI 402 Query: 421 TNYSQI---NDMFINEL----QPVETGHKTLE 445 +N++ I +M +N + + VE G + L+ Sbjct: 403 SNFAPIMKHQNMTVNAIYDRRKGVEEGLEELQ 434 >A5UZC1.1 A5UZC1_ROSS1 Extracellular solute-binding protein, family 1 Length = 488 Score = 197 bits (500), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 130/418 (31%), Positives = 225/418 (53%), Gaps = 28/418 (6%) Query: 61 SEDRTFIEKLIPDFEKEHPNIQVKVEKAPYEQFDSKLQTLIAGGKSPDV-TSHYGYGGFA 119 +E+R + ++P +++ P++ VKV + K + +IA + V +S++G GFA Sbjct: 75 TENRWERDVVLPAYQQVAPDVFVKVLNINQDDIAVKREAMIAAKEPLHVWSSNWGGDGFA 134 Query: 120 EYYNKDMLLDLTDLIKEDGFKPEDYHIPDNLMKIYTVKDRTYGIPVNMYVTLMLYNKDMF 179 + +L DLT LI+ D + D+ IP+ + IY V+ + YGIP + + YN +F Sbjct: 135 SDRFRGLLADLTPLIERDKWDTSDF-IPE-VFAIYNVEGKQYGIPFLTTGSYVYYNMKLF 192 Query: 180 DAAKLSYPPSDYEDKSWTFDKMVEEAKKMTLVSDDIAKTQY-GVDFTWAERDMRPLYFGA 238 D A + YPPSD+ DKSWT+D +E AKK+T DD + Y GV+ W D P+ +G Sbjct: 193 DEAGVPYPPSDWNDKSWTWDAFLETAKKLTKNPDDPSTAVYGGVNGLWPPFDSIPMIWGK 252 Query: 239 EPYSEDTWSNGGVPSETHFDSP-----KVIAAYHKLFDLIFKDKVSPTSEWSKSVAGQNG 293 +P++++ E+ F P + AA+ + DL++ KV+P S+++ Q G Sbjct: 253 DPFTKEA-------LESGFSDPIKLDEQTAAAFQAIHDLVYVHKVAPDQAASQAL-DQLG 304 Query: 294 DPFVAGKIGMSIGGS---WNLAGANDFP--FKIGVAAVPWGGND-KVRSTLFVDPLMILK 347 F++G++ M + G WN D P F G A +PWG D +R+T+F DP +I Sbjct: 305 GAFLSGRVAMFMTGGWGHWNYKEIIDDPNGFCWGAAPIPWGSPDANIRATIFTDPWVITA 364 Query: 348 --DSKHPKEAFEWIKYLVTTEVQEKSIELSGGNPPVNTEAAEAYYKHFEGIDPQD-VKKV 404 D+++ A+ ++K+L + E Q+++ L+ G PPV YYK +E P + K+ Sbjct: 365 GMDAENTDLAWNFVKFLASAE-QQRAYTLATGTPPVRQSLLNDYYKQYEKCVPAEKTKES 423 Query: 405 YEGAVKYGFESYNHLITNYSQINDMFINELQPV-ETGHKTLEEVMPVIQKKVTEIIKR 461 ++GA +G ES NHL+ + +++ + N L P + ++MP+++ V ++R Sbjct: 424 FQGAFSHGRESSNHLLVKFDELSQTWDNLLSPFWNDPNAKATDLMPILEADVNAALER 481 >A7NGQ3.1 A7NGQ3_ROSCS Extracellular solute-binding protein family 1 Length = 488 Score = 194 bits (494), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 130/417 (31%), Positives = 222/417 (53%), Gaps = 28/417 (6%) Query: 62 EDRTFIEKLIPDFEKEHPNIQVKVEKAPYEQFDSKLQTLIAGGKSPDV-TSHYGYGGFAE 120 E+R + ++P +++ P++ VKV + K + +IA + V +S++G GFA Sbjct: 76 ENRWERDVVLPAYQQVAPDVFVKVLNINQDDIAVKREAMIAAKEPLHVWSSNWGGDGFAS 135 Query: 121 YYNKDMLLDLTDLIKEDGFKPEDYHIPDNLMKIYTVKDRTYGIPVNMYVTLMLYNKDMFD 180 + +L DLT LI+ D + D+ IP+ + IY V+ + YGIP + + YN +FD Sbjct: 136 DRFRGLLADLTPLIERDKWDTSDF-IPE-VFGIYNVEGKQYGIPFLTTGSYVYYNMKLFD 193 Query: 181 AAKLSYPPSDYEDKSWTFDKMVEEAKKMTLVSDDIAKTQY-GVDFTWAERDMRPLYFGAE 239 A + YPPSD+ DKSWT+D +E AKK+T DD + Y GV+ W D P+ +G + Sbjct: 194 EAGVPYPPSDWNDKSWTWDAFLETAKKLTKNPDDPSTAVYGGVNGLWPPFDSIPMIWGKD 253 Query: 240 PYSEDTWSNGGVPSETHFDSP-----KVIAAYHKLFDLIFKDKVSPTSEWSKSVAGQNGD 294 P++ ET F P + AA+ + DL++ KV+P S+++ Q G Sbjct: 254 PFTAQA-------LETGFSDPIKLDEQTAAAFQAVHDLVYVHKVAPDQAASQAL-DQLGG 305 Query: 295 PFVAGKIGMSIGGS---WNLAGANDFP--FKIGVAAVPWGGNDK-VRSTLFVDPLMILK- 347 F++G++ M + G WN D P F G A +PWG D +R+T+F DP ++ Sbjct: 306 AFLSGRVAMFMTGGWGHWNYKDIIDDPNGFCWGAAPLPWGTPDATIRATIFTDPWVVTAG 365 Query: 348 -DSKHPKEAFEWIKYLVTTEVQEKSIELSGGNPPVNTEAAEAYYKHFEGIDPQD-VKKVY 405 D+++ A+ ++K+L + E Q+++ L+ G PPV YYK +E P + K+ + Sbjct: 366 MDAENTDMAWNFVKFLASAE-QQRAYTLATGTPPVRQSLLNDYYKQYEKCVPAEKTKESF 424 Query: 406 EGAVKYGFESYNHLITNYSQINDMFINELQPV-ETGHKTLEEVMPVIQKKVTEIIKR 461 +GA +G ES NHL+ + +++ + N L P + ++MP+++ V ++R Sbjct: 425 QGAFSHGRESSNHLLVKFDELSQTWDNLLSPFWNDPNAKASDLMPLLESDVNAALER 481 >C0UUV7.1 C0UUV7_9BACT Carbohydrate ABC transporter substrate-binding protein, CUT1 family Length = 510 Score = 190 bits (483), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 127/403 (31%), Positives = 213/403 (52%), Gaps = 19/403 (4%) Query: 72 PDFEKEHPNIQVKVEKAPYEQFDSKLQTLIAGGKSPDVTS-HYGYGGFAEYYNKDMLLDL 130 P+F+K HP I +K+ + K Q +IA G+ V S ++G GFA + ++ DL Sbjct: 107 PEFQKAHPEICLKILSINQDDIAVKRQAMIAAGEPLHVWSPNWGGDGFASDRIRGLIADL 166 Query: 131 TDLIKEDGFKPEDYHIPD-NLMKIYTVKDRTYGIPVNMYVTLMLYNKDMFDAAKLSYPPS 189 T LI+ D F D + D N++KIY + +T+ +P + + YNK++FD A + YPP+ Sbjct: 167 TPLIQRDNF---DLSVFDPNVLKIYQIDGKTWALPFLTTGSFVYYNKELFDKAGVPYPPT 223 Query: 190 DYEDKSWTFDKMVEEAKKMTLVSDDIAKTQYG-VDFTWAERDMRPLYFGAEPYSEDTWSN 248 ++D SWT+DK + AKK+T ++++ K Q+G + W D +G ++ Sbjct: 224 SWDDTSWTWDKFIATAKKLTKNTNNLNKAQFGAANGLWPPVDAIGALWGYWIIPDEA-QQ 282 Query: 249 GGVPSETHFDSPKVIAAYHKLFDLIFKDKVSPTSEWSKSVAGQNGDPFVAGKIGMSIGGS 308 G + + I A+ + DL++KDKV+P + + S Q G F +G++ M + G Sbjct: 283 TGFSDGVQVVNDRTIKAFQAMHDLVYKDKVAPNNA-AVSALTQLGGVFESGRVAMFMTGG 341 Query: 309 WNLAGAN---DFP--FKIGVAAVPWGGND-KVRSTLFVDPLMILKDSKHPKEAFEW--IK 360 W + D P F G A +P G D K R+ LF DP +I + ++ W IK Sbjct: 342 WGHWSYHPLIDDPNGFCWGAAPLPMGTPDAKRRAVLFTDPWVITANLSPEEQDLAWTFIK 401 Query: 361 YLVTTEVQEKSIELSGGNPPVNTEAAEAYYKHF-EGIDPQDVKKVYEGAVKYGFESYNHL 419 +LV+ + K +E + G PP T+ + YYK F + + P+D+K+V+EGA +G ES NHL Sbjct: 402 FLVSEDQARKYMEAT-GTPPTQTKLMQEYYKQFSKCMKPEDMKEVFEGAFSHGRESSNHL 460 Query: 420 ITNYSQINDMFINELQPV-ETGHKTLEEVMPVIQKKVTEIIKR 461 + + ++N ++ N L + E+V+ I+ + +KR Sbjct: 461 LVKFDELNQIWDNGLATFWNNPNAKAEDVLKKIEPQFNSALKR 503 >C2YQ38.1 C2YQ38_BACCE Extracellular solute-binding protein Length = 425 Score = 124 bits (312), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 112/412 (27%), Positives = 194/412 (47%), Gaps = 29/412 (7%) Query: 50 EQVTITHYTIDSEDRTFIEKLIPDFEKEHPNIQVKVEKAPYEQFDSKLQTLIAGGKSPDV 109 E++ + + D + +EK++ F ++HPNI+VK+E Y Q+ KL+T G PDV Sbjct: 42 EKIELRYALWDKKQLPAVEKVVQQFNEKHPNIKVKIELTAYGQYFQKLETAATGNALPDV 101 Query: 110 TSHYGYGGFAEYYNKDMLLDLTDLIKEDGFKPEDYHIPDNLMKIYTVKDRTYGIPVNMYV 169 G +Y + +L+ L D K+D +K E+Y P +L+ +YTV ++TYGIP + Sbjct: 102 FWMNG-PNVVKYASGKVLMPLDDKAKKDNYKLENY--PKSLVDLYTVDNKTYGIPKDFDT 158 Query: 170 TLMLYNKDMFDAAKLSYPPSDYEDKSWTFDKMVEEAKKMTLVSDDIAKTQYGVDFTWAER 229 T + YNK +FD A ++YP D SW ++KM E AK++T K + GV A Sbjct: 159 TALWYNKKLFDEAGVAYP-----DDSWDWEKMKEAAKQLT-------KKEKGVWGFAAAM 206 Query: 230 DMRPLYFGAEPYSEDTWSNGGVPSETHFDSPKVIAAYHKLFDLIFKDKVSPTSEWSKSVA 289 + Y+ Y + F+ P+ I A++ I K+ +SPT +++ Sbjct: 207 GNQGGYYDL-IYQNKGYVLSKDKKTVGFNKPQAIEAFNYNISFI-KEGLSPT--FAQMTE 262 Query: 290 GQNGDPFVAGKIGMSIGGSWNLAGANDFPFKIGVAAVPWGGNDKVRSTLFVDPLMILKDS 349 D F++GK+ M G W + P + +A +P G V + +I + Sbjct: 263 TTASDLFLSGKVAMIFDGPWMVPEYKKNP-DLNIAVMPKGKERAVMIHGLAN--VISAKT 319 Query: 350 KHPKEAFEWIKYLVTTEVQEKSIELSGGNPPVNTEAAEAYYKHFEGIDPQDVKKVYEGAV 409 K+P A+E++KYL + E + E +G P EA+ K ++ Q Y + Sbjct: 320 KYPDAAWEFVKYLGSKEAADVFAE-TGTVIPAFNGTQEAWVKSVPNLNLQ----AYITGL 374 Query: 410 KYGFESYNHLITNYSQINDMFINELQPVETGHKTLEEVMPVIQKKVTEIIKR 461 +Y + + + N + + L+ V G +EE + +K E++ + Sbjct: 375 EYSYPLPS--VKNTGALWEQETKVLKRVWGGELNVEEATKELTEKANEVLSK 424 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,824,143,073 Number of extensions: 125380084 Number of successful extensions: 230084 Number of sequences better than 1.0e-05: 1061 Number of HSP's gapped: 229677 Number of HSP's successfully gapped: 1063 Length of query: 461 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 322 Effective length of database: 1,751,175,118 Effective search space: 563878387996 Effective search space used: 563878387996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)