BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0034.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0TGI9.1 B0TGI9_HELMI Aspartate aminotransferase 351 9e-95 C0GGH5.1 C0GGH5_9FIRM Aminotransferase class I and II 348 4e-94 A3DDG1.1 A3DDG1_CLOTH L-aspartate aminotransferase EC=2.6.1.1 346 3e-93 B4BGZ1.1 B4BGZ1_CLOTM Aminotransferase class I and II 346 3e-93 A8MFC6.1 A8MFC6_ALKOO Aminotransferase class I and II 344 8e-93 >B0TGI9.1 B0TGI9_HELMI Aspartate aminotransferase Length = 397 Score = 351 bits (900), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 170/386 (44%), Positives = 240/386 (62%), Gaps = 1/386 (0%) Query: 15 VQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNFTGYTDTGGI 74 + PSPT +++ + + EG V G G+PDF TPD K AAI AI+ FT YT G Sbjct: 10 IHPSPTLSIDAKAKKMRSEGVDVIGFGAGEPDFDTPDHVKQAAIAAIEAGFTKYTPASGT 69 Query: 75 LPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIPIPYWVSFPE 134 L L++AIC L + G+ Y P +I+V+ G KH+L NA +C PGDEV+IP PYWVS+PE Sbjct: 70 LELKQAICDKLKAENGVDYAPANIIVSNGAKHSLVNAFEAICNPGDEVIIPAPYWVSYPE 129 Query: 135 QVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVYSAAELAKLA 194 VK A PVFV T S +K T E + + T+ L+LN P+NP+G VYS ELA++A Sbjct: 130 MVKLVDAVPVFVYTTEESRFKFTVEQIKQALTSKTKALVLNSPSNPTGMVYSKEELAEIA 189 Query: 195 EFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYGMTGWRIGYS 253 + + ++VISDE+Y ++ + SIAS ++E+T+++N VSK+Y MTGWRIGY+ Sbjct: 190 DLAVEYGIFVISDEIYEKLIYDDFEHVSIASINDKIKEQTIIVNGVSKAYSMTGWRIGYT 249 Query: 254 AAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRRSVLVEGVRR 313 A+ A + M +QSH TSNP GPQD + + GE+ RR +VE + Sbjct: 250 ASNAPIAKIMANIQSHATSNPNSIAQKAALAAITGPQDIVGTMVGEFVRRRDYMVERINA 309 Query: 314 IKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVMPGTGFGSPY 373 I GL C P+GAFYV ++ S G T G+ I+ +DD AD+LL++A VA++PG+GFG Sbjct: 310 IPGLSCLKPNGAFYVMMNISKIIGSTFAGRRINGSDDFADLLLEQAQVALVPGSGFGIDT 369 Query: 374 HIRLSYAVSRDEMESGIAAMESILGS 399 H+RLSYA S + + G+ +E+ L S Sbjct: 370 HVRLSYATSMENITEGLNRIENFLLS 395 >C0GGH5.1 C0GGH5_9FIRM Aminotransferase class I and II Length = 396 Score = 348 bits (894), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 166/392 (42%), Positives = 244/392 (62%), Gaps = 1/392 (0%) Query: 8 LADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNFTG 67 L+ + + PSPT +++ + + +G V G G+PDF TP+ K AA+ AI D FT Sbjct: 2 LSKKAQSISPSPTLAIDAKAKKMKADGVDVIGFGAGEPDFDTPEHIKDAAVSAIHDGFTR 61 Query: 68 YTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIPIP 127 YT G+L L++AIC L D GL+Y P +IVV+ G KH+L N L PGDEV+IP P Sbjct: 62 YTPVAGMLDLQQAICEKLQADNGLTYQPNEIVVSNGAKHSLTNVFAALLNPGDEVIIPAP 121 Query: 128 YWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVYSA 187 YWVS+ E VK PV V T A + +K+TP L V T+ +++N PNNP+G VY+A Sbjct: 122 YWVSYSEIVKLNDGVPVIVPTQAENNFKITPLELAGAVTSKTKAILINSPNNPTGQVYTA 181 Query: 188 AELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYGMT 246 EL +A+F R+L+V+SDE+Y ++ + SIAS +++ T+VIN VSKSY MT Sbjct: 182 KELQAVADFAVARNLYVVSDEIYEKLIYGEHEHVSIASLNEKIKDLTIVINGVSKSYAMT 241 Query: 247 GWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRRSV 306 GWRIGY+A A + M +QSH TSNP G Q + D++ ++ RR Sbjct: 242 GWRIGYTACRADIAKTMSSIQSHATSNPNSIAQKATIAALTGSQQCVADMKVAFSQRRDY 301 Query: 307 LVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVMPG 366 +V+ + I GL C P GAFYV++D S+ ++L GQ + T+D+ A +LL++A VAV+PG Sbjct: 302 MVDKISAIPGLSCIEPTGAFYVFMDVSSLFERSLNGQPVQTSDNFAALLLEQAKVAVVPG 361 Query: 367 TGFGSPYHIRLSYAVSRDEMESGIAAMESILG 398 TGFG+P ++RLSYA+ +++++G+ +E + Sbjct: 362 TGFGAPNYVRLSYALGMEQIKTGMTRIEDFIN 393 >A3DDG1.1 A3DDG1_CLOTH L-aspartate aminotransferase EC=2.6.1.1 Length = 395 Score = 346 bits (887), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 157/384 (40%), Positives = 236/384 (61%), Gaps = 1/384 (0%) Query: 15 VQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNFTGYTDTGGI 74 + PS T +++ + + EG + G G+PDF TPD K AAI AI FT YT G Sbjct: 10 ISPSSTLAIDAKAKKMRSEGIDIIGFGAGEPDFDTPDHIKKAAIDAINAGFTKYTPASGT 69 Query: 75 LPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIPIPYWVSFPE 134 L L++AICR RD GL Y+P +IV++ G KH+L NA+ +C PGDEV+IP P WVS+PE Sbjct: 70 LELKQAICRKFKRDNGLDYNPSNIVISNGAKHSLVNALQAICNPGDEVIIPTPAWVSYPE 129 Query: 135 QVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVYSAAELAKLA 194 VK A PV++ G+K T + L + + TR +I+N P+NP+G +YS EL +A Sbjct: 130 MVKLADGVPVYIHCSEEEGFKFTIDKLEKAITDKTRAIIINSPSNPTGMIYSEEELRAVA 189 Query: 195 EFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYGMTGWRIGYS 253 + + +++ISDE+Y ++ + SIASF +++ T+V+N VSKSY MTGWRIGY+ Sbjct: 190 DLAVSKGIYIISDEIYEKLIYDGYKHVSIASFNDKIKDLTIVVNGVSKSYAMTGWRIGYT 249 Query: 254 AAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRRSVLVEGVRR 313 A+ + M +QSH TSNP +GPQ+ ++++ E+ RR +V+ + Sbjct: 250 ASNEQIAKIMANVQSHATSNPNSIAQKAALAALEGPQEIIDEMSAEFVKRRDYMVDRINS 309 Query: 314 IKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVMPGTGFGSPY 373 + G+ C P+GAFYV ++ S G+ + G I+ +D A+ LL+KA VA++PG+GFG+ Sbjct: 310 MNGVSCIKPNGAFYVMMNISKLIGKEIAGMKITGSDSFAEALLEKANVALVPGSGFGTDI 369 Query: 374 HIRLSYAVSRDEMESGIAAMESIL 397 H+RLSYA S + + G+ +E L Sbjct: 370 HVRLSYATSMENIVEGLNRIEKFL 393 >B4BGZ1.1 B4BGZ1_CLOTM Aminotransferase class I and II Length = 395 Score = 346 bits (887), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 157/384 (40%), Positives = 236/384 (61%), Gaps = 1/384 (0%) Query: 15 VQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNFTGYTDTGGI 74 + PS T +++ + + EG + G G+PDF TPD K AAI AI FT YT G Sbjct: 10 ISPSSTLAIDAKAKKMRSEGIDIIGFGAGEPDFDTPDHIKKAAIDAINAGFTKYTPASGT 69 Query: 75 LPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIPIPYWVSFPE 134 L L++AICR RD GL Y+P +IV++ G KH+L NA+ +C PGDEV+IP P WVS+PE Sbjct: 70 LELKQAICRKFKRDNGLDYNPSNIVISNGAKHSLVNALQAICNPGDEVIIPTPAWVSYPE 129 Query: 135 QVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVYSAAELAKLA 194 VK A PV++ G+K T + L + + TR +I+N P+NP+G +YS EL +A Sbjct: 130 MVKLADGVPVYIHCSEEEGFKFTIDKLEKAITDKTRAIIINSPSNPTGMIYSEEELRAVA 189 Query: 195 EFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYGMTGWRIGYS 253 + + +++ISDE+Y ++ + SIASF +++ T+V+N VSKSY MTGWRIGY+ Sbjct: 190 DLAVSKGIYIISDEIYEKLIYDGYKHVSIASFNDKIKDLTIVVNGVSKSYAMTGWRIGYT 249 Query: 254 AAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRRSVLVEGVRR 313 A+ + M +QSH TSNP +GPQ+ ++++ E+ RR +V+ + Sbjct: 250 ASNEQIAKIMANVQSHATSNPNSIAQKAALAALEGPQEIIDEMSAEFVKRRDYMVDRINS 309 Query: 314 IKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVMPGTGFGSPY 373 + G+ C P+GAFYV ++ S G+ + G I+ +D A+ LL+KA VA++PG+GFG+ Sbjct: 310 MNGVSCIKPNGAFYVMMNISKLIGKEIAGMKITGSDSFAEALLEKANVALVPGSGFGTDI 369 Query: 374 HIRLSYAVSRDEMESGIAAMESIL 397 H+RLSYA S + + G+ +E L Sbjct: 370 HVRLSYATSMENIVEGLNRIEKFL 393 >A8MFC6.1 A8MFC6_ALKOO Aminotransferase class I and II Length = 397 Score = 344 bits (883), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 162/395 (41%), Positives = 243/395 (61%), Gaps = 1/395 (0%) Query: 5 HIQLADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDN 64 +IQL+ ++V PS T ++S + + +G V G+G+PDF TP+ K AAI+AI+ Sbjct: 2 NIQLSKKAQQVSPSITLAIDSKAKQMKADGINVISFGVGEPDFATPENIKNAAIEAIKKG 61 Query: 65 FTGYTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLI 124 FT YT G+ L++AIC L + L Y P +IVV+ GGKH+L+N +C PGDEV+I Sbjct: 62 FTTYTPASGLPQLKQAICNKLKAENNLDYKPENIVVSNGGKHSLYNIFQAICNPGDEVII 121 Query: 125 PIPYWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAV 184 P+PYWVS+P+ V A A PVFV + +K+ E L + T+ +ILN P+NP+GAV Sbjct: 122 PVPYWVSYPDMVAMADATPVFVHCTEENEFKLNKEDLLNAITSKTKAIILNSPSNPTGAV 181 Query: 185 YSAAELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSY 243 Y+ +EL ++A +R++++ISDE+Y V++ E + SIAS +++ T+V+N +SK Y Sbjct: 182 YTKSELEEIARIAVERNIFIISDEIYEKLVYEGEEHISIASLNDKIKDLTIVVNGMSKGY 241 Query: 244 GMTGWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATR 303 MTGWRIGY+A+ A + M +QSH TSNP G Q +E +R + R Sbjct: 242 AMTGWRIGYTASHAILAKGMGNIQSHATSNPNTIAQHASIEGLTGDQSSIEQMRVAFDER 301 Query: 304 RSVLVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAV 363 R +V+ + I GL C P GAFYV ++ G T++G TI + DL+++LL KA VAV Sbjct: 302 RKFMVQRINEIDGLSCIAPKGAFYVMMNIQKLLGTTIQGVTIHNSMDLSELLLDKANVAV 361 Query: 364 MPGTGFGSPYHIRLSYAVSRDEMESGIAAMESILG 398 +PG FG+ IRLSYA S + ++ + ++ +L Sbjct: 362 VPGIAFGTDEFIRLSYATSLENIQEALNRIDDLLN 396 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,438,819,352 Number of extensions: 102162309 Number of successful extensions: 272862 Number of sequences better than 1.0e-05: 9109 Number of HSP's gapped: 257693 Number of HSP's successfully gapped: 9117 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)