BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0031.p (391 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5XSB6.1 B5XSB6_KLEP3 Creatinase/peptidase, M24 family EC=3.4.-.- 313 2e-83 A9CL52.1 A9CL52_AGRT5 Peptidase 308 8e-82 A4WU80.1 A4WU80_RHOS5 Creatinase 294 1e-77 B9JLV8.1 B9JLV8_AGRRK Peptidase protein 293 2e-77 A3PPC3.1 A3PPC3_RHOS1 Creatinase 286 3e-75 >B5XSB6.1 B5XSB6_KLEP3 Creatinase/peptidase, M24 family EC=3.4.-.- Length = 389 Score = 313 bits (802), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 162/378 (42%), Positives = 226/378 (59%), Gaps = 4/378 (1%) Query: 8 FSRNEFHSRLHNVQSELGLKGLNGMLIHTPENIFYLTGYQSPGYYMYQCLVVPVSGDPVL 67 FS +E+ R+ + L G++ M++ PENIF+LTG Q+PGYY +Q L++PV GDPV Sbjct: 15 FSADEYAQRIRRTRERLSRAGVDVMIVTGPENIFWLTGQQTPGYYTFQALLLPVEGDPVF 74 Query: 68 VLRRGELSNVQTYSWLPESQVRTYDDTNDPVALTADTVRELSIAPGALGLETGSWFLPVR 127 V+R+ E N +++ ++ + Y D ++PV +++ +GL+ WFLPV Sbjct: 75 VIRQLEYFNFIANTFISDAAI--YQDGDNPVDFLVGMLQQRGWLHKRIGLDKRGWFLPVA 132 Query: 128 SYQKLEKMLGGFKLTDAQGTIEAFRVIKSPAEIAYIREACTVADRSMQAGLDAIRAGVNE 187 Y+ L+ LG + D G IE R +KS AE+ I A D M+AG+ AIR G +E Sbjct: 133 VYEALQARLG--TIADTAGVIEPLRAVKSAAEVEKIAIAARYVDAGMRAGMAAIRRGADE 190 Query: 188 DEXXXXXXXXXXXXGGEYLGMEPFISSGFRSGNMHAAWGHKVIGDGETLLLEVAGARNRY 247 + G EY+GMEP +S+G RSG H W +V+ D + + LE++ A +RY Sbjct: 191 NALVAAMMGAAIAAGSEYVGMEPLVSTGPRSGVPHGTWRRRVMQDNDPVFLEMSAAHDRY 250 Query: 248 HGALMRAAYTGTPTDEIKRVTGVCIASLNAALEVIRPGVTAGEVDEACRGTIERAGLYEQ 307 H A+MR+A+ G P + VC +L A+L+ IRPG T AC+ I+RAG E Sbjct: 251 HAAMMRSAWLGRPPAIAIEMEKVCQEALQASLDAIRPGATCEAPHLACQKVIDRAGFTEN 310 Query: 308 FRKRTGYSIGVAFAPDWGEGHIMSLQRDDSRVLLPGMVFHIPPALRVPNHYGVGFSETIL 367 F+KRTGYSIG+AFAPDWGEG I+SL R + L PGM FHIPPALR+ + VG SET++ Sbjct: 311 FKKRTGYSIGIAFAPDWGEGGILSLYRGVTTELQPGMTFHIPPALRIYGQFTVGVSETVV 370 Query: 368 VTETGCEVLTKLPRELTL 385 VTETG L L R LTL Sbjct: 371 VTETGYRQLGSLARPLTL 388 >A9CL52.1 A9CL52_AGRT5 Peptidase Length = 389 Score = 308 bits (788), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 155/378 (41%), Positives = 216/378 (57%), Gaps = 4/378 (1%) Query: 8 FSRNEFHSRLHNVQSELGLKGLNGMLIHTPENIFYLTGYQSPGYYMYQCLVVPVSGDPVL 67 F R E+ R + L GL+ +++ PENIFYL G Q+PGYY +Q L++P GDPV Sbjct: 15 FPREEYARRQALAREGLSAAGLDALIVTGPENIFYLCGQQTPGYYTFQALILPADGDPVF 74 Query: 68 VLRRGELSNVQTYSWLPESQVRTYDDTNDPVALTADTVRELSIAPGALGLETGSWFLPVR 127 ++R+ E N +++ ++ V Y D DP+AL + +A + ++ WFLP+ Sbjct: 75 LIRQLEYFNFIANTFISDAVV--YQDGEDPLALLVKLIESRGLASRRIAIDKRGWFLPIA 132 Query: 128 SYQKLEKMLGGFKLTDAQGTIEAFRVIKSPAEIAYIREACTVADRSMQAGLDAIRAGVNE 187 Y+ L+ LG + D G IE FR IKSP E+ + +A D M+AGL A+R G E Sbjct: 133 VYEALQARLG--TIGDGAGVIERFRAIKSPLEVEKLEKAAAYVDEGMRAGLAAVRVGATE 190 Query: 188 DEXXXXXXXXXXXXGGEYLGMEPFISSGFRSGNMHAAWGHKVIGDGETLLLEVAGARNRY 247 ++ G EYLGMEP +S G R+G H W + I DG+ LE+A +RY Sbjct: 191 NDLVSAMLGSAIAAGSEYLGMEPLVSVGPRTGVPHGTWRRRAITDGDPAFLEMAACHDRY 250 Query: 248 HGALMRAAYTGTPTDEIKRVTGVCIASLNAALEVIRPGVTAGEVDEACRGTIERAGLYEQ 307 H ALMR+A+ G P E + C +L +L+ IRPGV AC+ I+RAG + Sbjct: 251 HAALMRSAWIGEPPREAVDMMKACQEALQVSLDTIRPGVACEVPHIACQKVIDRAGYTDN 310 Query: 308 FRKRTGYSIGVAFAPDWGEGHIMSLQRDDSRVLLPGMVFHIPPALRVPNHYGVGFSETIL 367 F+KR GYS+G++FAPDWGEG I+SL L PGM FH+PPALR+ + VG SETI+ Sbjct: 311 FKKRLGYSVGISFAPDWGEGAILSLNTGVKTELQPGMTFHLPPALRIYGRFTVGVSETIV 370 Query: 368 VTETGCEVLTKLPRELTL 385 VTETG L + R L + Sbjct: 371 VTETGYRALGSIDRNLLI 388 >A4WU80.1 A4WU80_RHOS5 Creatinase Length = 394 Score = 294 bits (752), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 155/379 (40%), Positives = 219/379 (57%), Gaps = 4/379 (1%) Query: 7 IFSRNEFHSRLHNVQSELGLKGLNGMLIHTPENIFYLTGYQSPGYYMYQCLVVPVSGDPV 66 +F EF R+ +Q+ G +++ PENIF+ TG Q+ GY+ +Q LVVPV G PV Sbjct: 19 VFPAEEFAGRIRRLQTATADLGAEALVLTGPENIFWATGRQTAGYFAFQALVVPVEGAPV 78 Query: 67 LVLRRGELSNVQTYSWLPESQVRTYDDTNDPVALTADTVRELSIAPGALGLETGSWFLPV 126 L++R+ E + + +WL +R + D DP A A V +L + L +E +WF+ Sbjct: 79 LLVRQLETTGARAATWL--GDIRAWQDGEDPAAALAALVHDLGLR--RLAMERTAWFVSQ 134 Query: 127 RSYQKLEKMLGGFKLTDAQGTIEAFRVIKSPAEIAYIREACTVADRSMQAGLDAIRAGVN 186 ++ L G L D G E R +KSPAE+ IR A A+ ++ A ++A RAGV+ Sbjct: 135 ALADRIAAALAGVALIDGSGVAERLRAVKSPAELVAIRRAAGYAEAAIAASIEACRAGVS 194 Query: 187 EDEXXXXXXXXXXXXGGEYLGMEPFISSGFRSGNMHAAWGHKVIGDGETLLLEVAGARNR 246 E++ G E + MEP +SSG RSG HA W + + G+ + LE+A + +R Sbjct: 195 ENDVAAAMIGAAIRAGSEAMAMEPLVSSGPRSGIPHATWRRRTLASGDGVFLELAASHDR 254 Query: 247 YHGALMRAAYTGTPTDEIKRVTGVCIASLNAALEVIRPGVTAGEVDEACRGTIERAGLYE 306 YH ALMR+ + G P E R+ +L AALE +RPG EA + I+ AG Sbjct: 255 YHAALMRSVWIGPPPAEAVRMMDTAERALEAALEALRPGAPCSAPHEAAQAVIDAAGYTA 314 Query: 307 QFRKRTGYSIGVAFAPDWGEGHIMSLQRDDSRVLLPGMVFHIPPALRVPNHYGVGFSETI 366 FRKR GYS+G AFAPDWGEG I+SL + R+L PGMVFH+P LR Y VG SET+ Sbjct: 315 AFRKRIGYSMGAAFAPDWGEGAILSLFSGEDRLLEPGMVFHLPATLRSYGAYTVGASETV 374 Query: 367 LVTETGCEVLTKLPRELTL 385 +VT+TG EVL++LPR +T+ Sbjct: 375 IVTDTGIEVLSRLPRRMTV 393 >B9JLV8.1 B9JLV8_AGRRK Peptidase protein Length = 389 Score = 293 bits (750), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 148/376 (39%), Positives = 217/376 (57%), Gaps = 4/376 (1%) Query: 8 FSRNEFHSRLHNVQSELGLKGLNGMLIHTPENIFYLTGYQSPGYYMYQCLVVPVSGDPVL 67 F + E+ R+ + L G++ ++I PENIFYLTG Q+PGYY +Q L++P GDPV Sbjct: 15 FPKEEYEIRVARARQNLVEAGIDALVITGPENIFYLTGQQTPGYYTFQALLLPAEGDPVF 74 Query: 68 VLRRGELSNVQTYSWLPESQVRTYDDTNDPVALTADTVRELSIAPGALGLETGSWFLPVR 127 V+R+ E N +++ +++V Y D ++PV+ + + + + ++ WFLP+ Sbjct: 75 VIRQLEYFNFIANTFIADAEV--YQDGDEPVSFLMALIEKRGLRGKRVAIDKRGWFLPIF 132 Query: 128 SYQKLEKMLGGFKLTDAQGTIEAFRVIKSPAEIAYIREACTVADRSMQAGLDAIRAGVNE 187 Y+ L + LG + D G IE R +KSP EI + A + D M+AGL A++ G + Sbjct: 133 VYESLAERLG--VVHDGAGVIEKLRAVKSPLEIEKLERAASYVDAGMRAGLAAVKVGATD 190 Query: 188 DEXXXXXXXXXXXXGGEYLGMEPFISSGFRSGNMHAAWGHKVIGDGETLLLEVAGARNRY 247 ++ G EY+GMEP +S G R+G H W I DG+ LE+A +RY Sbjct: 191 NDLVAAMMSAAISAGSEYMGMEPLVSVGARTGVPHGTWRRGRIEDGDPAFLEMAACHDRY 250 Query: 248 HGALMRAAYTGTPTDEIKRVTGVCIASLNAALEVIRPGVTAGEVDEACRGTIERAGLYEQ 307 H ALMR+A+ G +E + VC +L +L+ IRPGV AC+ I+ AG + Sbjct: 251 HAALMRSAWIGQAPNEATEMMKVCQEALQVSLDTIRPGVPCEVPHMACQRIIDAAGYTDN 310 Query: 308 FRKRTGYSIGVAFAPDWGEGHIMSLQRDDSRVLLPGMVFHIPPALRVPNHYGVGFSETIL 367 FRKR GYS+G++FAPDWGEG I+SL L PGM FH+PPALR+ + VG SETI+ Sbjct: 311 FRKRLGYSVGISFAPDWGEGAILSLNAGVKTELRPGMTFHLPPALRIYGRFTVGVSETIV 370 Query: 368 VTETGCEVLTKLPREL 383 VT+TG L + R+L Sbjct: 371 VTDTGYRALGTVKRDL 386 >A3PPC3.1 A3PPC3_RHOS1 Creatinase Length = 394 Score = 286 bits (732), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 155/377 (41%), Positives = 225/377 (59%), Gaps = 4/377 (1%) Query: 7 IFSRNEFHSRLHNVQSELGLKGLNGMLIHTPENIFYLTGYQSPGYYMYQCLVVPVSGDPV 66 +F EF R+ +Q+ G + +++ PENIF+ TG Q+ GY+ +Q LVVPV G PV Sbjct: 19 VFPAAEFAGRMARLQAATADLGADALVLLGPENIFWATGRQTAGYFAFQALVVPVEGAPV 78 Query: 67 LVLRRGELSNVQTYSWLPESQVRTYDDTNDPVALTADTVRELSIAPGALGLETGSWFLPV 126 L++R+ E + + +WL + +R + D DP A VR+L + G + +E G+WF+ Sbjct: 79 LLVRQLETTGARASTWL--ADIRAWQDGEDPAAALGALVRDLGL--GRIAMERGAWFIGQ 134 Query: 127 RSYQKLEKMLGGFKLTDAQGTIEAFRVIKSPAEIAYIREACTVADRSMQAGLDAIRAGVN 186 +++ + L G L D G E R +KSPAE++ IR+A A+ ++ A ++A RAGV+ Sbjct: 135 DLSERIAQALAGVALIDGSGVAERLRAVKSPAELSAIRKAAGYAEAAIAASIEACRAGVS 194 Query: 187 EDEXXXXXXXXXXXXGGEYLGMEPFISSGFRSGNMHAAWGHKVIGDGETLLLEVAGARNR 246 E+E G E + MEP +SSG RSG HA W +++ G+ + LE+A + +R Sbjct: 195 ENEVAAAMMGAAIRAGSEAMAMEPLVSSGPRSGVPHATWRRRLLEPGDGVFLELAASHDR 254 Query: 247 YHGALMRAAYTGTPTDEIKRVTGVCIASLNAALEVIRPGVTAGEVDEACRGTIERAGLYE 306 YH ALMR+ + G P E R+ +L+AAL +RPG EA + I+ AG Sbjct: 255 YHAALMRSVWIGPPPAEAARMMDTAERALDAALAALRPGAPCAAPHEAAQAVIDAAGYTA 314 Query: 307 QFRKRTGYSIGVAFAPDWGEGHIMSLQRDDSRVLLPGMVFHIPPALRVPNHYGVGFSETI 366 FRKR GYS+G AFAPDWGEG I+SL R+L PGMVFH+P LR Y VG SET+ Sbjct: 315 AFRKRIGYSMGAAFAPDWGEGAILSLFTGVDRLLEPGMVFHLPATLRSYGDYTVGASETV 374 Query: 367 LVTETGCEVLTKLPREL 383 ++TETG EVL+ LPR++ Sbjct: 375 ILTETGIEVLSTLPRQM 391 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,343,931,548 Number of extensions: 98140667 Number of successful extensions: 222140 Number of sequences better than 1.0e-05: 2538 Number of HSP's gapped: 218968 Number of HSP's successfully gapped: 2541 Length of query: 391 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 253 Effective length of database: 1,760,596,133 Effective search space: 445430821649 Effective search space used: 445430821649 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)