BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0030.p (271 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1UDS4.1 C1UDS4_9ACTN Predicted amidohydrolase 254 4e-66 A4J4S3.1 A4J4S3_DESRM Nitrilase/cyanide hydratase and apolipopro... 211 4e-53 Q183H2.1 Q183H2_CLOD6 Putative carbon-nitrogen hydrolase 204 5e-51 B6X730.1 B6X730_9ENTR Putative uncharacterized protein 191 5e-47 B6XBM7.1 B6XBM7_9ENTR Putative uncharacterized protein 189 2e-46 >C1UDS4.1 C1UDS4_9ACTN Predicted amidohydrolase Length = 280 Score = 254 bits (650), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 134/265 (50%), Positives = 172/265 (64%), Gaps = 5/265 (1%) Query: 6 TVKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHEL 65 TV LALVQFES L D AAN +K R + +A+ +GA L+V PELF TGY +++ EL Sbjct: 12 TVHLALVQFESVLCDPAANTQKACRMIAEAAAEGADLVVLPELFSTGYELNIVGPHVPEL 71 Query: 66 AEDIDGPTVWAFREAAQTHNINIVIPMALKQSEGGTA-NGAVIIDRSGAIAGTYSKVHLW 124 AE +DGPTV A ++AA+ N +V +AL G N +V+IDR G + GTY K HLW Sbjct: 72 AEPVDGPTVRALQDAARAGNCYVVAGLALAYDMAGVPFNSSVVIDRQGELLGTYDKQHLW 131 Query: 125 EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 ER YF+ G C ++ DFG++GV+ICYD GFPE AR LALQG LI+ PSA+ ED Sbjct: 132 ALERFYFRSGCDCPVFDTDFGRIGVMICYDMGFPEVARMLALQGADLILCPSAWCQEDMD 191 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVL-- 242 WD+ PARALENT FVA +N G E L + G+ K+ NPRG ++ +L EEA VL Sbjct: 192 VWDVNAPARALENTAFVAAVNRYGVEDQLVMPGHTKVCNPRGHVV--AELAEEAEGVLHV 249 Query: 243 EIDLNEAAECSRQIPYLRDLRMDTY 267 E+DLNE + ++ PYLRD R D Y Sbjct: 250 ELDLNEVVDYRQRSPYLRDRRPDLY 274 >A4J4S3.1 A4J4S3_DESRM Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase Length = 273 Score = 211 bits (538), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 110/263 (41%), Positives = 155/263 (58%), Gaps = 1/263 (0%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 + + L+Q + L DVAANV K + + QA+ GA +I PEL TGY D++ + EL Sbjct: 5 LTIGLIQMDCVLGDVAANVAKAIERIRQAAAMGAQIICLPELCTTGYRPDLLEDKLWELT 64 Query: 67 EDIDGPTVWAFREAAQTHNINIVIPMALKQS-EGGTANGAVIIDRSGAIAGTYSKVHLWE 125 E + GPT F + A+ I I++PM K + G N AV ID+ G + G + K H + Sbjct: 65 EPVPGPTTDVFSQLAKELGIYIILPMNEKGAVPGMIHNSAVFIDKDGEVQGVFRKAHAYA 124 Query: 126 DERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKHR 185 ER YF G+ ++ +FGK+GV+ICYD GFPE AR L L+G ++I PSA+ ED+ Sbjct: 125 TERYYFTDGNHYPVFQTEFGKVGVMICYDMGFPEVARILTLKGAEVIFAPSAWRQEDEDI 184 Query: 186 WDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEID 245 WDI ARALEN FVA +N VG EG + + G +K+AN RG + EE + V +D Sbjct: 185 WDINIAARALENRLFVAAVNRVGREGDVVMHGKSKIANTRGKTLAEAARFEEDILVATVD 244 Query: 246 LNEAAECSRQIPYLRDLRMDTYG 268 L+E R+ PYL+D + +YG Sbjct: 245 LHELIAGRRETPYLKDRKPASYG 267 >Q183H2.1 Q183H2_CLOD6 Putative carbon-nitrogen hydrolase Length = 268 Score = 204 bits (520), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 106/263 (40%), Positives = 154/263 (58%), Gaps = 3/263 (1%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISH-RYHEL 65 VK+ ++Q S L +V N+ K + ++ +QGA +I PELF TGYN + + + EL Sbjct: 5 VKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLPELFATGYNLESLGGVKTLEL 64 Query: 66 AEDIDGPTVWAFREAAQTHNINIVIPM-ALKQSEGGTANGAVIIDRSGAIAGTYSKVHLW 124 + + + EAA+ +N+ ++ P L++ N AVI DR G I G Y K HLW Sbjct: 65 IREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVYNSAVIFDRKGKIMGEYCKNHLW 124 Query: 125 EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 E YFK G+ Y+ DFG+ GV+ICYDAGFPE +R L L+G ++I PSA+ ++D+ Sbjct: 125 SLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEVSRELTLKGSEIIFIPSAWRIQDED 184 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEI 244 WD+ RALENT + G+N V ++ L LFG +K+ NPRG +I D E + EI Sbjct: 185 MWDLNVSQRALENTVYTVGVNLVSNDSNLILFGKSKICNPRGTVITQLDTYREKYVLSEI 244 Query: 245 DLNEAAECSRQIPYLRDLRMDTY 267 DL+E + IPYL+D R +Y Sbjct: 245 DLDEIKKYREDIPYLKD-RKSSY 266 >B6X730.1 B6X730_9ENTR Putative uncharacterized protein Length = 277 Score = 191 bits (485), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 103/264 (39%), Positives = 153/264 (57%), Gaps = 2/264 (0%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 VK+AL QF+S L + N+++ + EQA+ QGA LI FPEL TGY D++S++ +L+ Sbjct: 6 VKVALAQFDSELGNKQRNLQRMAQLCEQAAAQGAKLICFPELATTGYRGDLLSNKLWDLS 65 Query: 67 EDIDGPTVWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGA--IAGTYSKVHLW 124 + T F E A N++IV A + S G +V + G I+G + KVH + Sbjct: 66 DSEGSETYCLFSELASRLNLSIVAGFAERGSYLGEVYNSVGLWNPGCKTISGVFRKVHAF 125 Query: 125 EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 E+++FK GD ++ GK+G++ICYD GFPE AR L LQG +L++ PSA+ ++D+ Sbjct: 126 GIEKQWFKNGDSFPVFDTPIGKVGIMICYDMGFPEVARILTLQGAELLIAPSAWCVQDRD 185 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEI 244 WDI RALEN + +N GSE L LFG +K+ PRG ++ + EA+ + E+ Sbjct: 186 MWDINTACRALENGTHLLAVNRWGSEEDLHLFGGSKVLGPRGQVLCEATCDGEALLIGEV 245 Query: 245 DLNEAAECSRQIPYLRDLRMDTYG 268 D A +PYLRD + YG Sbjct: 246 DFRLQAHTRLSVPYLRDRKPQRYG 269 >B6XBM7.1 B6XBM7_9ENTR Putative uncharacterized protein Length = 277 Score = 189 bits (481), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 106/267 (39%), Positives = 148/267 (55%), Gaps = 2/267 (0%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 VK+AL QF S L + N+++ + EQA+ QGA LI FPEL TGY D++S + +L+ Sbjct: 6 VKVALAQFNSELGNKTGNLQRMAQLCEQAASQGAKLICFPELATTGYRGDLLSTKLWDLS 65 Query: 67 EDIDGPTVWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGA--IAGTYSKVHLW 124 + T F + A +I IV A + G AV + G I+G + KVH + Sbjct: 66 DFEGSETDCLFSQLASRLDITIVSGFAERGERLGEVYNAVGVWNPGCENISGVFRKVHAF 125 Query: 125 EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 E+++FK GD +E GK+GV+ICYD GFPE AR L LQG +L++ PSA+ ++D+ Sbjct: 126 GIEKQWFKSGDSFPVFETPIGKIGVMICYDMGFPEVARILTLQGAELLIAPSAWCIQDRD 185 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEI 244 WDI RALEN + +N G E L LFG +K+ PRG ++ EA+ V EI Sbjct: 186 MWDINTACRALENGTHLLAVNRWGHEEDLHLFGGSKIVGPRGQVLCEASCESEALLVGEI 245 Query: 245 DLNEAAECSRQIPYLRDLRMDTYGYQA 271 D A +PYLRD + YG A Sbjct: 246 DFRLQAHTRLNVPYLRDRKPLNYGLLA 272 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,635,072,791 Number of extensions: 68676843 Number of successful extensions: 151588 Number of sequences better than 1.0e-05: 3450 Number of HSP's gapped: 145784 Number of HSP's successfully gapped: 3470 Length of query: 271 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 138 Effective length of database: 1,807,701,208 Effective search space: 249462766704 Effective search space used: 249462766704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)