BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0025.p (379 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZIC3.1 C0ZIC3_BREBN Putative muconate cycloisomerase 198 6e-49 O34508.1 O34508_BACSU YkfB EC=5.1.1.- 197 2e-48 Q65KW2.1 Q65KW2_BACLD YkfB 195 5e-48 A3I872.1 A3I872_9BACI Mandelate racemase/muconate lactonizing en... 193 3e-47 Q2B4A0.1 Q2B4A0_9BACI Putative uncharacterized protein 192 4e-47 >C0ZIC3.1 C0ZIC3_BREBN Putative muconate cycloisomerase Length = 364 Score = 198 bits (504), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 114/312 (36%), Positives = 181/312 (58%), Gaps = 13/312 (4%) Query: 1 MLISQIDVFRYDLPLSPPFTHSSSGTVTHLKEIYLRITADSGVCGWSEVRGNCEYVTGDT 60 M+I I+V R +PL PF ++ TV L+ + ++IT D+G+ GW E +TGD+ Sbjct: 1 MIIQAIEVKRISVPLKKPF-KTALRTVHSLESVLVKITCDNGMVGWGEASATV-VITGDS 58 Query: 61 PERVAAVLTHTLGPALLGKPVMNRNRINAGLDRLINGNSAARAAVDIALLDLTGKLLKVS 120 E + + + +T+ P L+G ++ R+ L + + GN++A+AAVDIAL DL + + Sbjct: 59 IESIESAIMNTMRPQLIGLNLLAYERVFQTLHQSMVGNTSAKAAVDIALYDLISQRAGMP 118 Query: 121 AAELLGGRMRDSIPSDATIAFGTPDEAEEEAARYLQDGFRTIKLRVGPDR-AGDLERVRR 179 + LGG RD + +D T++ +P E E+AA YL+ GF +K++VG D D+ R++ Sbjct: 119 LYQFLGG-YRDQLETDYTVSVNSPKEMGEDAAAYLKQGFHVLKIKVGKDNIEDDILRIQE 177 Query: 180 ARAVIESFGLGGEVLLCVDANQGWTPKQAVLRLREFE--ALGVEWVEQPVPADDFSGLKQ 237 R + G +V + +DANQGW K+AV +R+ E LG+E +EQPV A D GLK+ Sbjct: 178 IRKTV-----GNQVKIRLDANQGWNVKEAVQSIRKMEDMGLGIELIEQPVKAHDLEGLKR 232 Query: 238 VRQQTRAXXXXVADESCRTCADVARIAAEGAADIVHLKLVKAGSVVNLQRMMAVAESFGI 297 V +ADES + ++ AAD++++KL+KAG + Q + +AE +GI Sbjct: 233 VTDSVDTPI--MADESVFSPTQALQVLQNRAADMINIKLMKAGGIYKAQLINQLAEEYGI 290 Query: 298 PYMLGQMDEGRL 309 P M+G M E RL Sbjct: 291 PCMVGSMIESRL 302 >O34508.1 O34508_BACSU YkfB EC=5.1.1.- Length = 366 Score = 197 bits (500), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 126/364 (34%), Positives = 202/364 (55%), Gaps = 13/364 (3%) Query: 1 MLISQIDVFRYDLPLSPPFTHSSSGTVTHLKEIYLRITADSGVCGWSEVRGNCEYVTGDT 60 M I +I+ R +PL+ PF ++ TV + + +RIT DSG GW E +TGD+ Sbjct: 1 MKIIRIETSRIAVPLTKPF-KTALRTVYTAESVIVRITYDSGAVGWGEAPPTL-VITGDS 58 Query: 61 PERVAAVLTHTLGPALLGKPVMNRNRINAGLDRLINGNSAARAAVDIALLDLTGKLLKVS 120 + + + + H L PALLGK + I + L+ GN +A+AAV++AL D ++ + Sbjct: 59 MDSIESAIHHVLKPALLGKSLAGYEAILHDIQHLLTGNMSAKAAVEMALYDGWAQMCGLP 118 Query: 121 AAELLGGRMRDSIPSDATIAFGTPDEAEEEAARYLQDGFRTIKLRVGPDR-AGDLERVRR 179 ++LGG RD++ +D T++ +P+E +A YL+ GF+T+K++VG D A D+ R++ Sbjct: 119 LYQMLGG-YRDTLETDYTVSVNSPEEMAADAENYLKQGFQTLKIKVGKDDIATDIARIQE 177 Query: 180 ARAVIESFGLGGEVLLCVDANQGWTPKQAVLRLREFE--ALGVEWVEQPVPADDFSGLKQ 237 R + G V L +DANQGW PK+AV +R+ E LG+E VEQPV DD +GLK+ Sbjct: 178 IRKRV-----GSAVKLRLDANQGWRPKEAVTAIRKMEDAGLGIELVEQPVHKDDLAGLKK 232 Query: 238 VRQQTRAXXXXVADESCRTCADVARIAAEGAADIVHLKLVKAGSVVNLQRMMAVAESFGI 297 V T +ADES T + +AD++++KL+KAG + +++ A+AE+ G+ Sbjct: 233 VTDATDT--PIMADESVFTPRQAFEVLQTRSADLINIKLMKAGGISGAEKINAMAEACGV 290 Query: 298 PYMLGQMDEGRLATAALLQIASASRASHFEVWGFQRVRPEDDPAGVLTVLNGAVSVPVGF 357 M+G M E +L A A++ R + + D G +T +S+P Sbjct: 291 ECMVGSMIETKLGITAAAHFAASKRNITRFDFDAPLMLKTDVFNGGITYSGSTISMPGKP 350 Query: 358 GLGV 361 GLG+ Sbjct: 351 GLGI 354 >Q65KW2.1 Q65KW2_BACLD YkfB Length = 365 Score = 195 bits (496), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 135/366 (36%), Positives = 204/366 (55%), Gaps = 15/366 (4%) Query: 1 MLISQIDVFRYDLPLSPPFTHSSSGTVTHLKEIYLRITADSGVCGWSEVRGNCEYVTGDT 60 M I++I R +PL PF ++ TV + + +++TA++G+ GW E +TG+T Sbjct: 1 MKIAEITTNRIAVPLKKPF-KTALRTVRTAEAVIVKVTAENGMTGWGEAPPTA-VITGET 58 Query: 61 PERVAAVLTHTLGPALLGKPVMNRNRINAGLDRLINGNSAARAAVDIALLDLTGKLLKVS 120 + + + L PAL G + I +D+LI GN++A+AAV+ A+ D +L + Sbjct: 59 LDSIEGAVNTILAPALTGCNLRMAEAIFHDIDKLIVGNTSAKAAVETAVYDCLAQLCGLP 118 Query: 121 AAELLGGRMRDSIPSDATIAFGTPDEAEEEAARYLQDGFRTIKLRVGP-DRAGDLERVRR 179 +LLGG R+ I +D T++ P+E ++AARY+Q+GFRT+K++VG D A DLER+R Sbjct: 119 LYQLLGGYRRE-IETDFTVSVNDPEEMGKDAARYVQEGFRTLKIKVGKNDMATDLERIRE 177 Query: 180 ARAVIESFGLGGEVLLCVDANQGWTPKQAV--LRLREFEALGVEWVEQPVPADDFSGLKQ 237 R G V + VDANQGWT KQAV +R E E L +E VEQPV D GLKQ Sbjct: 178 IRK-----QAGPAVRIRVDANQGWTAKQAVKAIRKMEDEGLSIELVEQPVHRLDLEGLKQ 232 Query: 238 VRQQTRAXXXXVADESCRTCADVARIAAEGAADIVHLKLVKAGSVVNLQRMMAVAESFGI 297 V +ADES T D + +AD++++KL+KAG + ++ A+AE+ G+ Sbjct: 233 VTDAV--DTPIMADESVFTPRDAFHVLKTRSADLINIKLMKAGGIRQALKINAMAEACGV 290 Query: 298 PYMLGQMDEGRLA-TAALLQIASASRASHFEVWGFQRVRPEDDPAGVLTVLNGAVSVPVG 356 M G M E RL TAA+ AS + F+ + + ED G + G +++P Sbjct: 291 ECMAGSMIETRLGITAAVHFAASQKNVTKFD-FDAPLMLKEDIVEGGIRYHAGTITIPDA 349 Query: 357 FGLGVT 362 GLG+T Sbjct: 350 PGLGIT 355 >A3I872.1 A3I872_9BACI Mandelate racemase/muconate lactonizing enzyme family protein Length = 370 Score = 193 bits (490), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 118/381 (30%), Positives = 206/381 (54%), Gaps = 14/381 (3%) Query: 1 MLISQIDVFRYDLPLSPPFTHSSSGTVTHLKEIYLRITADSGVCGWSEVRGNCEYVTGDT 60 M I Q++++ LPL PF S T + I L++T D+G+ G+ E + E+VTG+T Sbjct: 1 MKIQQVEIYAIHLPLIDPFI-ISYATYDTMPSIILKVTTDTGIVGYGEAVPD-EHVTGET 58 Query: 61 PERVAAVLTHTLGPALLGKPVMNRNRINAGLDRLINGNSAARAAVDIALLDLTGKLLKVS 120 E AVL H L PA++G+ M +++ +++++ AA+AA+DIA D+ GK L V Sbjct: 59 WESTYAVLQHHLAPAVIGEDPMAFEKLHDKMNKIVKDVPAAKAAIDIACFDIAGKALGVP 118 Query: 121 AAELLGGRMRDSIPSDATIAFGTPDEAEEEAARYLQDGFRTIKLRVGPDRAGDLERVRRA 180 +LLGGR + P ++ GTP+ EEAA+ ++ G+R+ K++VG + D+ R++ Sbjct: 119 VYQLLGGRYHEKFPITHVLSIGTPEAMAEEAAKRVEMGYRSFKMKVGTEVTRDVARIKAV 178 Query: 181 RAVIESFGLGGEVLLCVDANQGWTPKQAVLR-LREFEALGVEWVEQPVPADDFSGLKQVR 239 R + G ++ + VD NQGW L+ LR + L ++W+EQPV ++D G+ +++ Sbjct: 179 RQQV-----GEDIAIRVDVNQGWENAATTLQGLRAMKDLNIDWLEQPVDSEDIDGMVEIK 233 Query: 240 QQTRAXXXXVADESCRTCADVARIAAEGAADIVHLKLVKAGSVVNLQRMMAVAESFGIPY 299 +++ + DE R ++ I A+ AAD V++KL+K G + ++ +AE GI Sbjct: 234 --SKSDVPLMIDEGIRGVREMREIIAKRAADKVNIKLMKCGGIYPAMKLAVMAEMAGIEC 291 Query: 300 MLGQMDEGRLATAALLQIASASR-ASHFEVWGFQRVRPEDDPAGVLTVLNGAVSVPVGFG 358 +G M E + +AA +A + + + E+ G + + G L + + G Sbjct: 292 QVGSMVESSVGSAAGFHVAFSKKIMTSVELTGPLKFSKD---VGSLHYDVPFICLNEQPG 348 Query: 359 LGVTVDESRLTRIACLSADAT 379 LG+ VDE+ + + S T Sbjct: 349 LGIDVDEAVMKELCKFSTVVT 369 >Q2B4A0.1 Q2B4A0_9BACI Putative uncharacterized protein Length = 369 Score = 192 bits (488), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 112/325 (34%), Positives = 183/325 (56%), Gaps = 13/325 (4%) Query: 1 MLISQIDVFRYDLPLSPPFTHSSSGTVTHLKEIYLRITADSGVCGWSEVRGNCEYVTGDT 60 M I +I+ FR +PL PF ++ TVT + + ++IT D+G+ GW E +TGD+ Sbjct: 1 MNIERIETFRVAIPLIKPF-KTALRTVTTAESVVIKITCDNGIEGWGEAPPTV-VITGDS 58 Query: 61 PERVAAVLTHTLGPALLGKPVMNRNRINAGLDRLINGNSAARAAVDIALLDLTGKLLKVS 120 + + + H P L+GK ++N I + L+ GNS+A+AAVD+AL D K ++ Sbjct: 59 LMSIESAIHHVFKPYLVGKSLLNYETIFQAIKTLMVGNSSAKAAVDMALYDCLSKHCRLP 118 Query: 121 AAELLGGRMRDSIPSDATIAFGTPDEAEEEAARYLQDGFRTIKLRVGPDR-AGDLERVRR 179 + LGG ++ I +D T++ P+E ++AA Y+ GF +K++VG D D+ R++ Sbjct: 119 LYQFLGG-AKNEIETDYTVSVNNPEEMGDDAASYIDQGFNVLKVKVGKDDIMTDISRIKE 177 Query: 180 ARAVIESFGLGGEVLLCVDANQGWTPKQAVLRLREFE--ALGVEWVEQPVPADDFSGLKQ 237 R + G +V + +DANQGW K AV +R+ E L +E VEQPVPADD GLKQ Sbjct: 178 IRRRV-----GNKVKIRLDANQGWQAKDAVRAIRKMEDSLLDIELVEQPVPADDIEGLKQ 232 Query: 238 VRQQTRAXXXXVADESCRTCADVARIAAEGAADIVHLKLVKAGSVVNLQRMMAVAESFGI 297 V +ADES + ++ +AD++++KL+KAG + Q + +AE+ G+ Sbjct: 233 VTDSVET--LIMADESVFSPKQALQVLCTRSADLINIKLMKAGGIFQAQAINQLAETCGV 290 Query: 298 PYMLGQMDEGRLATAALLQIASASR 322 M+G M E ++ +A A++ + Sbjct: 291 ECMVGSMIETKIGISAAAHFAASKK 315 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,164,465,423 Number of extensions: 88379811 Number of successful extensions: 217238 Number of sequences better than 1.0e-05: 2178 Number of HSP's gapped: 213171 Number of HSP's successfully gapped: 2178 Length of query: 379 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 242 Effective length of database: 1,770,017,148 Effective search space: 428344149816 Effective search space used: 428344149816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)