BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0008.p (377 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GMP0.1 B7GMP0_ANOFW L-alanine dehydrogenase 537 e-151 Q08352.1 DHA_BACSU Alanine dehydrogenase EC=1.4.1.1 532 e-149 A9VUJ6.1 A9VUJ6_BACWK Alanine dehydrogenase EC=1.4.1.1 531 e-149 A4ITR4.1 A4ITR4_GEOTN Alanine dehydrogenase 530 e-148 B4BPE6.1 B4BPE6_9BACI Alanine dehydrogenase 530 e-148 >B7GMP0.1 B7GMP0_ANOFW L-alanine dehydrogenase Length = 376 Score = 537 bits (1383), Expect = e-151, Method: Compositional matrix adjust. Identities = 269/372 (72%), Positives = 309/372 (83%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVAITPAGV+S V++GH V++E AG GSGF NE+Y AGA++I Sbjct: 1 MIIGVPKEIKNNENRVAITPAGVMSFVSNGHTVIIEKDAGIGSGFTNEDYVQAGAQIIER 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A+ VWA A+MVMKVKEPL SEY YFRPGL+LFTYLHLA EP LA ALK+ GV AI YETV Sbjct: 61 AADVWAQADMVMKVKEPLPSEYEYFRPGLVLFTYLHLAAEPELARALKEKGVIAIAYETV 120 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 GRTLPLLTPMSEVAGRM+ Q+GAQFL+K GG+GILLSGVP T Sbjct: 121 QVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLSGVPGVSRGKVTIIGGGVVGT 180 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAK+AIGLGA VTI+DLSA+RLR+LDDIFG+QI+TL+SNP NIA+AVAE+DL+IGAVLI Sbjct: 181 NAAKVAIGLGANVTIIDLSAERLRELDDIFGSQINTLMSNPMNIAQAVAESDLVIGAVLI 240 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAPKLVTEEMVK M+PGSVIVDVAIDQGGIVET D VTTHD+P + K+GV+HY+VAN Sbjct: 241 PGAKAPKLVTEEMVKAMQPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVAN 300 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVT+PYALQIANKGV KA+ DN L+ GVNVANG IT AVA LG Sbjct: 301 MPGAVPRTSTLALTNVTMPYALQIANKGVQKAVADNPALRLGVNVANGEITYEAVARDLG 360 Query: 361 EEYYTVEQALEQ 372 +Y VE AL++ Sbjct: 361 YDYVPVEVALDK 372 >Q08352.1 DHA_BACSU Alanine dehydrogenase EC=1.4.1.1 Length = 378 Score = 532 bits (1371), Expect = e-149, Method: Compositional matrix adjust. Identities = 267/371 (71%), Positives = 301/371 (81%), Gaps = 1/371 (0%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVA+TP GV L+++GH+VLVE GAG GSGF NE Y +AGAE+I + Sbjct: 1 MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIAD 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 VW A EMVMKVKEPL EY YFR GL+LFTYLHLA EP LA ALKD GV AI YETV Sbjct: 61 PKQVWDA-EMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETV 119 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 +GRTLPLLTPMSEVAGRM+ Q+GAQFL+K GG+GILL+GVP T Sbjct: 120 SEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGT 179 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAKMA+GLGA+VTI+DL+ADRLRQLDDIFG QI TLISNP NIA AVAEADLLI AVLI Sbjct: 180 NAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLI 239 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAP LVTEEMVK MKPGSVIVDVAIDQGGIVET+D +TTHD P +EK+GV+HY+VAN Sbjct: 240 PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVAN 299 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVTVPYALQIANKG KA+ DN L++G+N ANG +T AVA LG Sbjct: 300 MPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLG 359 Query: 361 EEYYTVEQALE 371 EY E+AL+ Sbjct: 360 YEYVPAEKALQ 370 >A9VUJ6.1 A9VUJ6_BACWK Alanine dehydrogenase EC=1.4.1.1 Length = 377 Score = 531 bits (1367), Expect = e-149, Method: Compositional matrix adjust. Identities = 266/370 (71%), Positives = 296/370 (80%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVA+TPAGV+ V GHKVLVE AG GSGF N +Y AGAE++ Sbjct: 1 MIIGVPKEIKNNENRVALTPAGVIPFVTAGHKVLVETNAGVGSGFRNVDYVYAGAEIVES 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A+ VW +EM+MKVKEPLESEY +FRP LILFTYLHLA E LA ALKD V AI YETV Sbjct: 61 AAEVWNQSEMIMKVKEPLESEYIHFRPNLILFTYLHLAAESNLAQALKDKRVTAIAYETV 120 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 T PLLTPMSEVAGRMSVQ+GAQFLQK+ GGQGILL+GVP T Sbjct: 121 EVNHTRPLLTPMSEVAGRMSVQIGAQFLQKSNGGQGILLAGVPGVNRGKIAIIGGGIVGT 180 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAKMAIGLGA+VTI+DL+ADRLRQLDDIFG QI TL+SNP+NIA+AV +ADLLI AVLI Sbjct: 181 NAAKMAIGLGADVTIIDLNADRLRQLDDIFGTQIKTLMSNPFNIAEAVKQADLLISAVLI 240 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAPKLVTEEMV+ MKPGSVIVDVAIDQGG VET D VTTHD+P FEKY VLHYSVAN Sbjct: 241 PGAKAPKLVTEEMVQRMKPGSVIVDVAIDQGGAVETCDHVTTHDNPTFEKYEVLHYSVAN 300 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVT+PYALQIANKGV AI +N LK GVN NG ITC VA+ LG Sbjct: 301 MPGAVPRTSTIALTNVTIPYALQIANKGVQTAIFENDALKLGVNTLNGEITCEVVAKELG 360 Query: 361 EEYYTVEQAL 370 +Y ++E+A Sbjct: 361 YKYVSIEEAF 370 >A4ITR4.1 A4ITR4_GEOTN Alanine dehydrogenase Length = 377 Score = 530 bits (1364), Expect = e-148, Method: Compositional matrix adjust. Identities = 266/370 (71%), Positives = 304/370 (82%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVAITPAGV+S V GHKVL+E AG GSGF +++Y AGA++I Sbjct: 1 MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIER 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A VWA A+MVMKVKEPL SEY YFRPGLILFTYLHLA EP L ALK++GV AI YETV Sbjct: 61 AEDVWAQADMVMKVKEPLPSEYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETV 120 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 GRTLPLLTPMSEVAGRM+ Q+GAQFL+K YGG+GILLSGVP T Sbjct: 121 QVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLSGVPGVARGKVTIIGGGVVGT 180 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAK+A+GLGA+VTI+DL+ADRLR+LDDIFG QI+TL+SNP NIA+AVAEADL+IGAVLI Sbjct: 181 NAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI 240 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGA+APKLVTE+MVK MKPGSVIVDVAIDQGGIVET D VTTHD+P + K+GV+HY+VAN Sbjct: 241 PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVAN 300 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVT+PYALQIANKGV +AI DN L+ G+NVANG IT AVA LG Sbjct: 301 MPGAVPRTSTIALTNVTIPYALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLG 360 Query: 361 EEYYTVEQAL 370 Y +AL Sbjct: 361 YRYVPAREAL 370 >B4BPE6.1 B4BPE6_9BACI Alanine dehydrogenase Length = 377 Score = 530 bits (1364), Expect = e-148, Method: Compositional matrix adjust. Identities = 266/370 (71%), Positives = 304/370 (82%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVAITPAGV+S V GHKVL+E AG GSGF +++Y AGA++I Sbjct: 1 MIIGVPKEIKNNENRVAITPAGVLSFVQAGHKVLIEKEAGVGSGFSDDDYIRAGAQIIER 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A VWA A+MVMKVKEPL SEY YFRPGLILFTYLHLA EP L ALK++GV AI YETV Sbjct: 61 AEDVWAQADMVMKVKEPLPSEYGYFRPGLILFTYLHLAAEPELTRALKESGVIAIAYETV 120 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 GRTLPLLTPMSEVAGRM+ Q+GAQFL+K YGG+GILLSGVP T Sbjct: 121 QVGRTLPLLTPMSEVAGRMAAQIGAQFLEKPYGGKGILLSGVPGVARGKVTIIGGGVVGT 180 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAK+A+GLGA+VTI+DL+ADRLR+LDDIFG QI+TL+SNP NIA+AVAEADL+IGAVLI Sbjct: 181 NAAKVAVGLGADVTIIDLNADRLRELDDIFGHQITTLMSNPMNIAEAVAEADLVIGAVLI 240 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGA+APKLVTE+MVK MKPGSVIVDVAIDQGGIVET D VTTHD+P + K+GV+HY+VAN Sbjct: 241 PGARAPKLVTEDMVKAMKPGSVIVDVAIDQGGIVETSDHVTTHDNPTYVKHGVVHYAVAN 300 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVT+PYALQIANKGV +AI DN L+ G+NVANG IT AVA LG Sbjct: 301 MPGAVPRTSTIALTNVTIPYALQIANKGVIQAIADNPALELGLNVANGEITYEAVARDLG 360 Query: 361 EEYYTVEQAL 370 Y +AL Sbjct: 361 YRYVPAREAL 370 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,050,814,449 Number of extensions: 80607781 Number of successful extensions: 252084 Number of sequences better than 1.0e-05: 1764 Number of HSP's gapped: 248670 Number of HSP's successfully gapped: 1952 Length of query: 377 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 240 Effective length of database: 1,770,017,148 Effective search space: 424804115520 Effective search space used: 424804115520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)