BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0007.p (418 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8ELK2.1 Q8ELK2_OCEIH Hypothetical conserved protein 225 8e-57 Q65FF2.1 Q65FF2_BACLD YukF 224 1e-56 A7Z8A2.1 A7Z8A2_BACA2 YukF 223 3e-56 B7GMN9.1 B7GMN9_ANOFW DNA-binding transcriptional regulator (Con... 223 4e-56 P71073.2 YUKF_BACSU Uncharacterized protein yukF 220 2e-55 >Q8ELK2.1 Q8ELK2_OCEIH Hypothetical conserved protein Length = 415 Score = 225 bits (573), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 124/394 (31%), Positives = 213/394 (54%), Gaps = 3/394 (0%) Query: 14 SMESLADTLSESLQSQVTIEDSNHHVIGYSSHQFESDPARISTIIGKRVPNAVIIGLRKK 73 S LAD++++ + +TIEDSNHHVI YS H+ + D RISTII ++VP+ VI KK Sbjct: 18 SPNQLADSIADLFECPITIEDSNHHVISYSQHKDDIDQVRISTIINRKVPDKVINAFWKK 77 Query: 74 GVMHQLENTAHPIRIPAVMEVGLGPRLAMCIRHHQEILGYIWVVDRGNLAEGHAERVVEK 133 G M +L ++ P+ IP + EVGLG R+A+ +R EILG+IW + ++ Sbjct: 78 GYMPKLIDSDDPVVIPEIQEVGLGNRIAISVRKQNEILGFIWAHTSDKTFTEEEVFLFKE 137 Query: 134 AAGIAGRYLLKQRGWKTRQEKAHEDFFWKLLTSHYETEPRIRQEAEGFSILLPESYYIGV 193 AA +Y LK R + E+ + DFFW+LL I ++A+ ++I L I V Sbjct: 138 AATYVKKYFLKNRQHNRKSEEGYRDFFWQLLRGDLHNTDDINRQAKQYNIQLNGDKTIVV 197 Query: 194 LESDKTIDERFIQRFRQLAETDASQRLLFLTAEHNRLILLFSFAFPVEDPELLSSYVHKL 253 + ++E+ + L+E+ ++ + N I+L +LL ++ +L Sbjct: 198 IRFSDEVNEQMEKHAYYLSESQVKVNVITRLFDDNDFIMLVGLPKESNSKQLLYDFIQQL 257 Query: 254 IQELGQ--SESCVLTAGCSPGYREYLSAAAAYREALTILEVKKLLPYHARHLLLYEEIGF 311 IQ +G+ + + VL AG Y + ++ +A +L++++ +P +LLYE++G Sbjct: 258 IQRIGEQLNLNTVLGAG-GLRYEQPQLIEQSFHQAKNVLKLQEQIPERLNDILLYEDLGV 316 Query: 312 WAYIPAIMEQXXXXXXXXXLLYPLKAHDREHKSDFLKTIAVYLTLNGNLKEAAAFLHIHT 371 +I + E + L+++D+ H++ L+T+ +YL + N+ AA L +H Sbjct: 317 LQFIDTLAETRKDEHYYNPYMERLRSYDKRHQTSLLETLDIYLQCDSNVYRAAKELFVHP 376 Query: 372 NTLMYRLNRIAEITGRSLKETDYRTSVYLDILTE 405 NT+ YRL RI E++G LK + +T++YLD+L + Sbjct: 377 NTMNYRLKRIKEVSGIDLKNANQKTNIYLDLLID 410 >Q65FF2.1 Q65FF2_BACLD YukF Length = 412 Score = 224 bits (570), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 131/407 (32%), Positives = 216/407 (53%), Gaps = 7/407 (1%) Query: 1 MTDSEPSFDRFFDSMESLADTLSESLQSQVTIEDSNHHVIGYSSHQFESDPARISTIIGK 60 M + F +D +E +AD +SE L +TIED NH ++ YS+H +DPAR STIIG+ Sbjct: 1 MNNRNNPFKYQYDRLEDVADHISEVLDCPITIEDINHRLLAYSTHSDYTDPARTSTIIGR 60 Query: 61 RVPNAVIIGLRKKGVMHQLENTAHPIRIPAVMEVGLGPRLAMCIRHHQEILGYIWVVDRG 120 RVP VI L K G + L T PIR+ + EVGL R+A+ I + E++G+IW ++ Sbjct: 61 RVPEKVINKLWKDGTIPALMKTDEPIRVEQIDEVGLSSRVAISIWKNSEVIGFIWALESQ 120 Query: 121 NLAEGHAERVVEKAAGIAGRYLLKQRGWKTRQEKAHEDFFWKLLTSHYETEPRIRQEAEG 180 +++ AA LL + K + E+ ++FFWKLLT H + I Sbjct: 121 KTLSEDELHLLKLAADSVKNKLLPYQVRKRKNEQRSQEFFWKLLTGHISEDQEISDGFHQ 180 Query: 181 FSILLPESYYIGVLESDKTIDERFIQRFRQLAETDASQRLLFLTAEHNRLILLFS----- 235 I +P +Y + ++ I+E+ ++ L ET ++L T ++N LI+L S Sbjct: 181 LGIGVPSTYSVIIIRLKDEIEEKTEKQLHYLLETTQQLQILLATIDYNELIVLASPKTGQ 240 Query: 236 FAFPVEDPELLSSYVHKLIQELGQSESCVLTAGCSPGYREYLSAAAAYREALTILEVKKL 295 P D + ++ + K ++E + +C + G Y +Y+EAL+ L+VK+ Sbjct: 241 KGQPFNDLKQFAANIQKQLEERYKLNNCTIAIGGM--YSLITLVHQSYQEALSALKVKER 298 Query: 296 LPYHARHLLLYEEIGFWAYIPAIMEQXXXXXXXXXLLYPLKAHDREHKSDFLKTIAVYLT 355 P RHL+ + E+G + Y+ + E+ L L+A+D+EH S+ ++T+ ++ Sbjct: 299 FPDETRHLVSFSELGIYQYLDVLSEKRKHASYPNYSLMQLEAYDKEHHSNLVETLEQFIE 358 Query: 356 LNGNLKEAAAFLHIHTNTLMYRLNRIAEITGRSLKETDYRTSVYLDI 402 + N+ AA L+IH NTL YRL RI++I LK + + ++YL+I Sbjct: 359 CDSNVNTAAKKLNIHVNTLNYRLKRISKIAEIDLKNINEKFTIYLEI 405 >A7Z8A2.1 A7Z8A2_BACA2 YukF Length = 409 Score = 223 bits (568), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 137/408 (33%), Positives = 221/408 (54%), Gaps = 12/408 (2%) Query: 1 MTDSEPSFDRFFDSMESLADTLSESLQSQVTIEDSNHHVIGYSSHQFESDPARISTIIGK 60 MT+ + F FD +E +AD +SE LQ +TIED NH ++ YS+H +DPAR STIIG+ Sbjct: 1 MTNPKDPFKYSFDRLEDVADHISEVLQCPITIEDVNHKLLAYSTHSDCTDPARTSTIIGR 60 Query: 61 RVPNAVIIGLRKKGVMHQLENTAHPIRIPAVMEVGLGPRLAMCIRHHQEILGYIWVVDRG 120 RVP VI L K G + L T PI++ + EVGL R+A+ I + ++LG+IW ++ Sbjct: 61 RVPEKVINKLWKDGTIPALLKTDQPIKVKEIDEVGLSNRVAISIWKNSQVLGFIWALEIK 120 Query: 121 NLAEGHAERVVEKAAGIAGRYLLKQRGWKTRQEKAHEDFFWKLLTSHYETEPRIRQEAEG 180 + ++ AA LL + KT+ E+ ++FFWK+LT H E + AEG Sbjct: 121 KKLNDEDLKTLQLAAKAVRNKLLHLQIRKTKIEERGQEFFWKMLTGHIYQEAEM---AEG 177 Query: 181 FSIL---LPESYYIGVLESDKTIDERFIQRFRQLAETDASQRLLFLTAEHNRLILLFSFA 237 F L LP + + ++ + E+ Q+ + L ET ++ T + N LILL + Sbjct: 178 FHKLGMALPSVFSVMIIRLHDELTEKTEQQLQYLQETTQQIHVMLATVDSNELILLAAPK 237 Query: 238 F--PVEDPELLSSYVHKLIQELGQSESCVLTAGCSPGYREYLS-AAAAYREALTILEVKK 294 P +D + + ++ K ++E + E + G G E +S +Y+EAL++L+ K+ Sbjct: 238 TDQPFKDVKHFALHIQKQLKERYKIEDASIAVG---GIYESISYVYRSYQEALSVLKAKE 294 Query: 295 LLPYHARHLLLYEEIGFWAYIPAIMEQXXXXXXXXXLLYPLKAHDREHKSDFLKTIAVYL 354 +HL + E+G + Y+ + E+ L L+ +DREH++D +KT+ ++ Sbjct: 295 RFVRETKHLFSFSELGIYQYLDVLDEKRKHSGYMNYSLSKLEQYDREHQADMVKTLEHFI 354 Query: 355 TLNGNLKEAAAFLHIHTNTLMYRLNRIAEITGRSLKETDYRTSVYLDI 402 + N+ AA L+IH NTL YRL RI+ I LK + + ++YLDI Sbjct: 355 DADSNVNTAAKALNIHVNTLNYRLKRISHIAEIDLKNVNEKFTIYLDI 402 >B7GMN9.1 B7GMN9_ANOFW DNA-binding transcriptional regulator (Contains C-terminal Fis domain) Length = 410 Score = 223 bits (567), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 124/401 (30%), Positives = 217/401 (54%), Gaps = 1/401 (0%) Query: 3 DSEPSFDRFFDSMESLADTLSESLQSQVTIEDSNHHVIGYSSHQFESDPARISTIIGKRV 62 D F F+S+E AD +SE LQ +TIED+NH +I YS H+ +DPAR +TII +RV Sbjct: 2 DVMNPFATHFESLEEFADRVSELLQCPITIEDANHRLIAYSGHEHYTDPARTATIIHRRV 61 Query: 63 PNAVIIGLRKKGVMHQLENTAHPIRIPAVMEVGLGPRLAMCIRHHQEILGYIWVVDRGNL 122 P VI L ++GV+ +L + P+R+ + EVGLG R+A+ I ++E+LG+IW ++ Sbjct: 62 PERVINSLWREGVIPKLLQSEEPVRVTEISEVGLGNRIAISIWKNKEVLGFIWAIEMNES 121 Query: 123 AEGHAERVVEKAAGIAGRYLLKQRGWKTRQEKAHEDFFWKLLTSHYETEPRIRQEAEGFS 182 H ++++AA A LL+ + K ++E+ ++FFWKLLT H+ + I ++ + Sbjct: 122 WTEHQFDLLKQAAKAAKNLLLQLQIRKHKKEERIQEFFWKLLTGHFSSAHDIVEQCHQLN 181 Query: 183 ILLPESYYIGVLESDKTIDERFIQRFRQLAETDASQRLLFLTAEHNRLILLFSFAFPVED 242 + + + +G+ + + I + ++ L +T + T +H RLI+L + + V Sbjct: 182 VHVYPFFSVGIFQFQQHIQTQVERQIDYLLKTTQQLAIPLYTIDHQRLIVLVAPSSNVYG 241 Query: 243 PELLSSYVHKLIQELGQSESCVLTAGCSPG-YREYLSAAAAYREALTILEVKKLLPYHAR 301 + + + ++ + +T G Y Y A +Y+EA T+L++K+ P+ Sbjct: 242 EAHIERFFRSFMTQMKERFRIDITMGAFGNVYECYTKIAKSYKEAQTVLKMKRAFPHQLA 301 Query: 302 HLLLYEEIGFWAYIPAIMEQXXXXXXXXXLLYPLKAHDREHKSDFLKTIAVYLTLNGNLK 361 ++ Y+ +G + + + EQ L LK +D +H ++ ++TI YL + N+ Sbjct: 302 NIQSYQHLGMYQFFDLLFEQKRHGDFHNVALEKLKQYDAQHHTNLVETIETYLDCDSNVN 361 Query: 362 EAAAFLHIHTNTLMYRLNRIAEITGRSLKETDYRTSVYLDI 402 E A L+IH NTL YRL RI+EI L + +Y+D+ Sbjct: 362 ETAKTLNIHPNTLAYRLKRISEIGNIDLSNMSEKVKLYIDM 402 >P71073.2 YUKF_BACSU Uncharacterized protein yukF Length = 422 Score = 220 bits (561), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 131/407 (32%), Positives = 217/407 (53%), Gaps = 10/407 (2%) Query: 1 MTDSEPSFDRFFDSMESLADTLSESLQSQVTIEDSNHHVIGYSSHQFESDPARISTIIGK 60 MT F FD +E +AD +S+ L+ +TIED NH ++ YS+H +DPAR STIIG+ Sbjct: 14 MTMPNDPFKYSFDRLEDVADHISDVLRCPITIEDVNHKLLAYSTHSDCTDPARTSTIIGR 73 Query: 61 RVPNAVIIGLRKKGVMHQLENTAHPIRIPAVMEVGLGPRLAMCIRHHQEILGYIWVVDRG 120 RVP VI L K G + L T PIR+ + EVGL R+A+ I ++++LG+IW ++ Sbjct: 74 RVPEKVINKLWKDGTIPALLKTDQPIRVKQIDEVGLSNRVAISIWKNKQVLGFIWALEIQ 133 Query: 121 NLAEGHAERVVEKAAGIAGRYLLKQRGWKTRQEKAHEDFFWKLLTSHYETEPRIRQEAEG 180 ++ AA LLK + KT+ E+ ++FFWK+LT H E + A+G Sbjct: 134 KTLSDEDLLTLQMAAKAVKNKLLKLQIRKTKNEERSQEFFWKMLTGHIHQEDDM---ADG 190 Query: 181 FSIL---LPESYYIGVLESDKTIDERFIQRFRQLAETDASQRLLFLTAEHNRLILLFS-- 235 F L P + + ++ + + E+ Q+ + L ET +L T + N LI+L S Sbjct: 191 FHKLGMAAPSEFSVMIIRINGELTEKIEQQLQYLQETTQQVYVLLATVDSNELIILTSPK 250 Query: 236 FAFPVEDPELLSSYVHKLIQELGQSESCVLTAGCSPGYREYLSAAAAYREALTILEVKKL 295 P +D + + K ++E + E + G Y + +Y+EAL++L+ K+ Sbjct: 251 TDHPFQDLKQFALSTQKQLKERYKIEDVSIAFGGI--YNSISFVSRSYQEALSVLKTKER 308 Query: 296 LPYHARHLLLYEEIGFWAYIPAIMEQXXXXXXXXXLLYPLKAHDREHKSDFLKTIAVYLT 355 +HL + E+G + Y+ + E+ L L+ +DR+H+S+ ++T+ ++ Sbjct: 309 FAEETKHLFSFSELGIYQYLDVLNEKRKQAGHYNYSLSKLEQYDRDHQSNMVETLERFIE 368 Query: 356 LNGNLKEAAAFLHIHTNTLMYRLNRIAEITGRSLKETDYRTSVYLDI 402 + N+ A+ L+IH NTL YRL RI++I LK + + ++YLDI Sbjct: 369 ADSNVNTASKLLNIHVNTLNYRLKRISQIAEIDLKNVNQKFTIYLDI 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,311,489,362 Number of extensions: 90373783 Number of successful extensions: 220436 Number of sequences better than 1.0e-05: 248 Number of HSP's gapped: 220363 Number of HSP's successfully gapped: 250 Length of query: 418 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 280 Effective length of database: 1,760,596,133 Effective search space: 492966917240 Effective search space used: 492966917240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)