BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0003.p (630 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0K338.1 B0K338_THEPX Histidine kinase internal region 212 1e-52 Q62YW8.1 Q62YW8_BACLD Putative Histidine kinase 198 2e-48 Q65NG8.1 Q65NG8_BACLD Putative uncharacterized protein 197 2e-48 Q2BFM1.1 Q2BFM1_9BACI Putative uncharacterized protein 194 2e-47 A9KL84.1 A9KL84_CLOPH Integral membrane sensor signal transducti... 194 4e-47 >B0K338.1 B0K338_THEPX Histidine kinase internal region Length = 567 Score = 212 bits (539), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 142/539 (26%), Positives = 259/539 (48%), Gaps = 17/539 (3%) Query: 47 PTTIISVTVYNKSADIITRNVHTSIENNLNLVQDNLAQRFAAADNAMISLYLNTEFADLI 106 P +++ N ++ I V + LN N+++ NA++ + +N E + Sbjct: 17 PLIMVAAITGNITSRYINEEVKKTTFQTLNQANKNISKMLENMKNAILYVSMNKELQYNL 76 Query: 107 SSNRPTDSTGIINELAAL-NKILEXXXXXXXXXXXXVPMLYMLNRPEYTQYNFSRRVFNI 165 S N I E+ A+ N IL + + L++ +Y V + Sbjct: 77 SRNGGETPFQINREVTAIRNSILYPGIFNENYSSIEI---FALHKNQYPMRLEQNDVMSS 133 Query: 166 DLISLKPWYLGIPARSDFTVVGLSSLDSRFTLKFAKRLFGIRHAQLPYVGLLTIDIPVTE 225 ++ K WY + ++ + + ++ +F I++ P + ++++D+ +++ Sbjct: 134 KVVENKEWYKKTIQLNGRLYWYINDDFGKQMISVSRLVFDIKNFAKP-IAVISVDMDMSK 192 Query: 226 FDNLLDHYKPTAGSRIYIADQTGTIAISPDAALLGQNISTQDYYSQMKTAEGGGTLHSFR 285 +++ ++Y+ D G + S D + L + Y +++ + G + Sbjct: 193 VASVVSDIHLGKTGKVYLVDDKGKLIYSEDKSFLYR------YTTKLYNSSSGSDFIT-- 244 Query: 286 QVIQGGNMLVSYIKIPETGWTILSFSPVKELNGELASFQRVMYXXXXXXXXXXXXXXXXX 345 I G ++V Y +P+ GW ++ + ELN + + +Y Sbjct: 245 --INGNKIMVIYNTLPQNGWKLVGMVSMAELNEKAEMIRNFIYLTALLSLVIAALISLYF 302 Query: 346 XENISAPIRKFIQSMSHAESGNFNIIIRYRRKDEFSYLFNRYNKLLQQIKALIDKLYVTE 405 +IS PI K M E GNFNI + E L++ +N ++++I LI ++Y+++ Sbjct: 303 SYSISQPIIKLATEMKKVEKGNFNISVEENWDGEIGVLYSSFNYMIERINELIHEVYLSK 362 Query: 406 LRKKEAELKTLQAQINPHFLYNTLDSINWIAINHDIPEISNMVTSLSDFFRYSLSKGRNI 465 +++K+AELK LQAQINPHFLYNTLD++NW+A+ H++PEIS +V SL+ RYS++KG ++ Sbjct: 363 IKEKDAELKALQAQINPHFLYNTLDTVNWLAVKHNVPEISKIVNSLASILRYSINKGNDV 422 Query: 466 IPLRDELRQVDSYLQIQQFRFQDKLEYELEETDPKVLEECLVVKLSLQPLVENAXXXXXX 525 + +EL+ V+SY+ IQ+ RF+DK E D +VL +KL LQPLVENA Sbjct: 423 TTVENELKHVESYITIQKIRFKDKFEVSF-NIDKRVL-HYKTIKLILQPLVENAIIHGIE 480 Query: 526 XXXXXXXXXXXXXXSQDILCVSVFDDGVGADPERLNQLLTDKQPGNESYGIRNVHTRIQ 584 + + V ++G D + +N+LL +SYGI+NV+ RI+ Sbjct: 481 TYEGKGKILINGYLDGEKIVFEVINNGNPIDLDLVNKLLDSPADDKDSYGIQNVNERIK 539 >Q62YW8.1 Q62YW8_BACLD Putative Histidine kinase Length = 589 Score = 198 bits (503), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 112/278 (40%), Positives = 159/278 (57%), Gaps = 8/278 (2%) Query: 348 NISAPIRKFIQSMSHAESGNFNIIIRYRRKDEFSYLFNRYNKLLQQIKALIDKLYVTELR 407 ++S PI K M AESGNF+ + + R+DE L +N+L+ +I+ L+ E + Sbjct: 317 SMSRPILKLNAVMKTAESGNFSTLYQEEREDEIGLLGKSFNRLMFRIRELMSVTERQERQ 376 Query: 408 KKEAELKTLQAQINPHFLYNTLDSINWIAINHDIPEISNMVTSLSDFFRYSLSKGRNIIP 467 K+EAEL+ LQAQINPHFLYNTLD+INW+A E+S++V +LS FR SLSKG++IIP Sbjct: 377 KREAELRALQAQINPHFLYNTLDTINWMARKKGAEEVSDLVQALSKLFRISLSKGKDIIP 436 Query: 468 LRDELRQVDSYLQIQQFRFQDKLEYELEETDPKVLEECLVVKLSLQPLVENAXXXXXXXX 527 L DE V++YL+IQ+ R++DKL Y +E T + ++ L++KL LQP+VENA Sbjct: 437 LSDEFEHVENYLKIQKARYRDKLNYSIEMTG--LCDDRLILKLVLQPIVENAIYHGIKEK 494 Query: 528 XXXXXXXXXXXXSQDILCVSVFDDGVGADPERLNQLLTDK---QPGNESYGIRNVHTRIQ 584 L + + DDG G D E L Q +TD+ + YG+ NVH RI Sbjct: 495 KGPGHIAIKGMEEDGSLIIKIEDDGAGMDEETLKQ-ITDQFKHRDDTAGYGMINVHERIA 553 Query: 585 QFFGEAYGIRYYANDEEQRGLLAVVRFPVVTTWNEVME 622 FGE YGI + G +R P++ +E Sbjct: 554 LTFGEPYGIT--VESRKGAGTAVTIRLPILAKGGTSLE 589 >Q65NG8.1 Q65NG8_BACLD Putative uncharacterized protein Length = 594 Score = 197 bits (502), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 112/278 (40%), Positives = 159/278 (57%), Gaps = 8/278 (2%) Query: 348 NISAPIRKFIQSMSHAESGNFNIIIRYRRKDEFSYLFNRYNKLLQQIKALIDKLYVTELR 407 ++S PI K M AESGNF+ + + R+DE L +N+L+ +I+ L+ E + Sbjct: 322 SMSRPILKLNAVMKTAESGNFSTLYQEEREDEIGLLGKSFNRLMFRIRELMSVTERQERQ 381 Query: 408 KKEAELKTLQAQINPHFLYNTLDSINWIAINHDIPEISNMVTSLSDFFRYSLSKGRNIIP 467 K+EAEL+ LQAQINPHFLYNTLD+INW+A E+S++V +LS FR SLSKG++IIP Sbjct: 382 KREAELRALQAQINPHFLYNTLDTINWMARKKGAEEVSDLVQALSKLFRISLSKGKDIIP 441 Query: 468 LRDELRQVDSYLQIQQFRFQDKLEYELEETDPKVLEECLVVKLSLQPLVENAXXXXXXXX 527 L DE V++YL+IQ+ R++DKL Y +E T + ++ L++KL LQP+VENA Sbjct: 442 LSDEFEHVENYLKIQKARYRDKLNYSIEMTG--LCDDRLILKLVLQPIVENAIYHGIKEK 499 Query: 528 XXXXXXXXXXXXSQDILCVSVFDDGVGADPERLNQLLTDK---QPGNESYGIRNVHTRIQ 584 L + + DDG G D E L Q +TD+ + YG+ NVH RI Sbjct: 500 KGPGHIAIKGMEEDGSLIIKIEDDGAGMDEETLKQ-ITDQFKHRDDTAGYGMINVHERIA 558 Query: 585 QFFGEAYGIRYYANDEEQRGLLAVVRFPVVTTWNEVME 622 FGE YGI + G +R P++ +E Sbjct: 559 LTFGEPYGIT--VESRKGAGTAVTIRLPILAKGGTSLE 594 >Q2BFM1.1 Q2BFM1_9BACI Putative uncharacterized protein Length = 588 Score = 194 bits (493), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 126/425 (29%), Positives = 203/425 (47%), Gaps = 24/425 (5%) Query: 200 AKRL-FGIRHAQLPYVGLLTIDIPVTEFDNLLDHYKPTAGSRIYIADQTGTIAISPDAAL 258 A RL +GI ++ +G LT+ + + ++ + Y PT I I DQ + ++ Sbjct: 169 ANRLIYGIHSYKMDEIGYLTMSMKTSYLEDRIQKYDPTEERHIIILDQENNVVLTNAED- 227 Query: 259 LGQNISTQDYYSQMKTAEGGGTL--HSFRQVIQGGNMLVSYIKIPETGWTILSFSPVKEL 316 S D ++ + ++ SF + G M+V+ + TGW I+S + E+ Sbjct: 228 -----SGNDAFTSWASDLDYNSIDSESFVDIPGDGRMIVNNYQSELTGWKIISLVSLNEI 282 Query: 317 NGELASFQRVMYXXXXXXXXXXXXXXXXXXENISAPIRKFIQSMSHAESGNFNIIIRYRR 376 + R ++ I P+ + M E NF++ ++ R Sbjct: 283 SAGPVLIGRTIFLIGFVAIVIGIVVIWISTHYIVKPLNQLRLVMDEVEKDNFHVQVQINR 342 Query: 377 KDEFSYLFNRYNKLLQQIKALIDKLYVTELRKKEAELKTLQAQINPHFLYNTLDSINWIA 436 KDE + + +NK++ +I LI +Y ++ +KEA+L+ L+AQINPHFLYNTLD+INW+A Sbjct: 343 KDELGRVGDSFNKMMNKINNLISDIYQKDINEKEAQLRALRAQINPHFLYNTLDAINWMA 402 Query: 437 INHDIPEISNMVTSLSDFFRYSLSKGRNIIPLRDELRQVDSYLQIQQFRFQDKLEYELEE 496 E+S M +LS + S+S + I L +E + +D Y+ IQ+ RFQDK+ Y ++ Sbjct: 403 QFDKTDEVSKMTIALSRLLKSSISNNKEFIRLEEEAKYIDDYMTIQKIRFQDKIHYSMQI 462 Query: 497 TDPKVLEECLVVKLSLQPLVENAXXXXXXXXXXXXXXXXXXXXSQDILCVSVFDDGVGAD 556 + + CLV KL LQP+VENA D+L + + D+GVG D Sbjct: 463 DEAA--KNCLVPKLILQPIVENAMIHGLEKKLENGYLLIKGYVKNDLLHIQIIDNGVGMD 520 Query: 557 PERLNQLLT--------DKQPGNESYGIRNVHTRIQQFFGEAYGIRYYANDEEQRGLLAV 608 ++ LL +K GN GI NV RI+ FG YG+ +N E G + Sbjct: 521 ERTMSALLKGVYVNRDENKGTGN---GILNVQNRIKLLFGREYGLNIDSN--ENVGSVFE 575 Query: 609 VRFPV 613 + PV Sbjct: 576 LTLPV 580 >A9KL84.1 A9KL84_CLOPH Integral membrane sensor signal transduction histidine kinase Length = 580 Score = 194 bits (492), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 120/340 (35%), Positives = 181/340 (53%), Gaps = 12/340 (3%) Query: 281 LHSFRQVIQGGNMLVSYIKIP-ETGWTILSFSPVKELNGELASFQRVMYXXXXXXXXXXX 339 L SF++ +G +V Y IP GW++ + + +KELN + + Sbjct: 234 LFSFKEE-KGKEQVVFYSPIPYSNGWSLCTMTSLKELNLHTQNLIYTILVIWILMLAISI 292 Query: 340 XXXXXXXENISAPIRKFIQSMSHAESGNFNII--IRYRRKDEFSYLFNRYNKLLQQIKAL 397 I+API K ++M + G+F++ + +KDE ++L N + + +QI L Sbjct: 293 VSCFLFSRTITAPIYKLSKAMQLVKDGSFDLPKQVISGKKDELAFLANNFYTMTEQISFL 352 Query: 398 IDKLYVTELRKKEAELKTLQAQINPHFLYNTLDSINWIAINHDIPEISNMVTSLSDFFRY 457 ++++ E+ K+ AE + LQ+QINPHFLYNTLD++ W A +H+ E+S M+ +LSDFFR Sbjct: 353 MERIKEEEVAKRTAEFQVLQSQINPHFLYNTLDNLQWRAYDHNALELSEMIEALSDFFRI 412 Query: 458 SLSKGRNIIPLRDELRQVDSYLQIQQFRFQDKLEYELEETDPKVLEECLVVKLSLQPLVE 517 SLS G+ I + E+ V SYL IQQ R++D L + + DP V E + +KL +QPLVE Sbjct: 413 SLSDGKEFISVIKEIEHVKSYLFIQQKRYEDILNFTI-SCDPDV-SEYVTIKLIVQPLVE 470 Query: 518 NAXXXXXXXXXXXXXXXXXXXXSQDILCVSVFDDGVGADPERLNQLLTDKQ---PGNESY 574 NA +D + + V DDGVG P +L LL + PG + Y Sbjct: 471 NALYHGIKPKLSHGTIAVRVLRKEDTIQIIVQDDGVGMSPPKLKTLLRSLEQDAPG-KGY 529 Query: 575 GIRNVHTRIQQFFGEAYGIRYYANDEEQRGLLAVVRFPVV 614 G+ N+H RIQ +G+ YGI EEQ G A + P V Sbjct: 530 GLYNIHRRIQMVYGKEYGIS--ITSEEQIGTTATIIIPAV 567 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,196,181,644 Number of extensions: 122913955 Number of successful extensions: 305256 Number of sequences better than 1.0e-05: 1951 Number of HSP's gapped: 301753 Number of HSP's successfully gapped: 1951 Length of query: 630 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 488 Effective length of database: 1,722,912,073 Effective search space: 840781091624 Effective search space used: 840781091624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)