BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0011.p (374 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KCW8.1 B3KCW8_9BACI Aminomethyltransferase EC=2.1.2.10 510 e-142 Q9K934.1 GCST_BACHD Aminomethyltransferase EC=2.1.2.10 468 e-130 Q2B5T1.1 Q2B5T1_9BACI Aminomethyltransferase EC=2.1.2.10 452 e-125 C5D4A2.1 C5D4A2_9BACI Glycine cleavage system T protein 450 e-124 C0ZBW5.1 C0ZBW5_BREBN Aminomethyltransferase EC=2.1.2.10 449 e-124 >B3KCW8.1 B3KCW8_9BACI Aminomethyltransferase EC=2.1.2.10 Length = 370 Score = 510 bits (1313), Expect = e-142, Method: Compositional matrix adjust. Identities = 245/368 (66%), Positives = 293/368 (79%), Gaps = 4/368 (1%) Query: 1 MEALKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMV 60 M +L+RTP Y LYS + +RCIDFGGWELPVQF+GIV+EHEAVR+ AGLFDVSHMGEFM+ Sbjct: 1 MSSLQRTPLYPLYSDFPSTRCIDFGGWELPVQFSGIVQEHEAVRKHAGLFDVSHMGEFMI 60 Query: 61 SGSGSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNI 120 SG +EAF+Q MTTNDV+R+ G AQYTLM GG VDDLLVY+L +R+MLVVNASNI Sbjct: 61 SGQDAEAFIQNMTTNDVTRITVGQAQYTLMCNDKGGTVDDLLVYKLSSDRFMLVVNASNI 120 Query: 121 DKDFEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTSAPIAELAPFHFIER 180 DKD +WL EH+T + V + NVS ET L+ALQGP AE IL++ TS + ++ FHFI+ Sbjct: 121 DKDLQWLHEHVTGD---VAIRNVSAETALIALQGPAAENILSKATSEMLGDIPSFHFIQN 177 Query: 181 AVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFE 240 A VCG LLSRTGYTGEDGFE+Y A +W GLL AG HGL PAGLGARDTLRFE Sbjct: 178 AQVCGHAALLSRTGYTGEDGFEIYCSAADAPDIWRGLLTAGKDHGLIPAGLGARDTLRFE 237 Query: 241 AKLPLYGQELSADITPLEAGVQFFVKLDKAGFIGRDALLKQKESGLPRRLVGLEMIDRGI 300 AKLPLYGQELS+ I+PLEA + +FVKLD FIGR+AL +QK+ G+PR+LVG+E+IDRGI Sbjct: 238 AKLPLYGQELSSTISPLEASLGYFVKLDSGDFIGREALQQQKQDGVPRKLVGIELIDRGI 297 Query: 301 PRSHYPVY-ADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAAV 359 PRSHYPV +G IGEVT+GTQSP+LKRNLGLAL++ Y +EV+VEIRGK+LKA V Sbjct: 298 PRSHYPVLNGNGEPIGEVTSGTQSPSLKRNLGLALIETPYASLDSEVWVEIRGKKLKAKV 357 Query: 360 VKTPFYKK 367 VKTPFYK+ Sbjct: 358 VKTPFYKR 365 >Q9K934.1 GCST_BACHD Aminomethyltransferase EC=2.1.2.10 Length = 365 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 224/367 (61%), Positives = 281/367 (76%), Gaps = 4/367 (1%) Query: 1 MEALKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMV 60 M LK+TP +DLY Y + IDFGGW LPVQF+ I +EHEAVR +AGLFDVSHMGE V Sbjct: 1 MTELKKTPLFDLYEQYG-GKVIDFGGWALPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEV 59 Query: 61 SGSGSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNI 120 +G+ + +LQ + TNDVS+++DG AQYT M Y GG VDDLL+YR E++Y+LV+NA+NI Sbjct: 60 TGAQALNYLQRLVTNDVSKIKDGQAQYTAMCYENGGTVDDLLIYRRSEDQYLLVINAANI 119 Query: 121 DKDFEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTSAPIAELAPFHFIER 180 DKD W+++H D GV++TNVS++T LALQGP+AE +L +T P+A++ F F++ Sbjct: 120 DKDIAWMEKHAID---GVSITNVSNQTAQLALQGPVAENVLQTLTEEPLADIKFFRFVDG 176 Query: 181 AVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFE 240 + GV VLLSRTGYTGEDGFELY + A VLW L+ AG HG+ P GLGARDTLRFE Sbjct: 177 VNIAGVNVLLSRTGYTGEDGFELYCLAEDAPVLWKKLIEAGKEHGVVPCGLGARDTLRFE 236 Query: 241 AKLPLYGQELSADITPLEAGVQFFVKLDKAGFIGRDALLKQKESGLPRRLVGLEMIDRGI 300 AKLPLYGQEL+ DI+P+EAG+ F VK+DK FIG++ L KQKE G PR+LVGLEM+D+GI Sbjct: 237 AKLPLYGQELTKDISPIEAGIGFAVKVDKEDFIGKEILKKQKEQGAPRKLVGLEMVDKGI 296 Query: 301 PRSHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAAVV 360 PR+ Y VY D KIG VTTGTQSPTLK+N+GLALL A ++E GTEV V +R +QL A VV Sbjct: 297 PRTGYEVYVDNQKIGFVTTGTQSPTLKKNVGLALLQAEHSELGTEVIVHVRKRQLIAKVV 356 Query: 361 KTPFYKK 367 TPFYK+ Sbjct: 357 ATPFYKR 363 >Q2B5T1.1 Q2B5T1_9BACI Aminomethyltransferase EC=2.1.2.10 Length = 368 Score = 452 bits (1164), Expect = e-125, Method: Compositional matrix adjust. Identities = 222/371 (59%), Positives = 276/371 (74%), Gaps = 6/371 (1%) Query: 1 MEALKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMV 60 M LKRTP Y+ Y ++ IDFGGW+LPVQFTGI EHEAVR +AGLFDVSHMGE V Sbjct: 1 MSQLKRTPLYEEYRKLG-AKTIDFGGWDLPVQFTGIKDEHEAVRTKAGLFDVSHMGEIEV 59 Query: 61 SGSGSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNI 120 G S+ FLQ M TND+S+L+ GGAQYT M Y +GG VDDLLVY+ ++ Y+LVVNA+NI Sbjct: 60 KGQASQDFLQKMMTNDISKLKSGGAQYTAMCYESGGTVDDLLVYKFEDDHYLLVVNAANI 119 Query: 121 DKDFEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVT-SAPIAELAPFHFIE 179 +KD++WL++HL D G + N+SD+T LALQGP AE IL ++ ++E+ F F Sbjct: 120 EKDYQWLEDHLID---GADIRNLSDQTAQLALQGPAAEGILQKLAGEKDLSEIGFFKFSS 176 Query: 180 RAVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRF 239 + G + L+SRTGYTGEDGFE+Y D A+ +WN +L AG G+ P GLG RDTLRF Sbjct: 177 DVDLNGKKALVSRTGYTGEDGFEIYCHSDDASSIWNDILEAGKEEGVLPCGLGCRDTLRF 236 Query: 240 EAKLPLYGQELSADITPLEAGVQFFVKLDK-AGFIGRDALLKQKESGLPRRLVGLEMIDR 298 EA L LYGQELS D+TPLEAG+ F VK++K A FIG+ L +QKE+G+PR+LVGLEMIDR Sbjct: 237 EANLALYGQELSPDVTPLEAGIGFAVKINKEADFIGKPVLKQQKENGVPRKLVGLEMIDR 296 Query: 299 GIPRSHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAA 358 GIPR YPV ADG ++GEVTTGTQSPTLK+N+GLAL+ Y E G EV VEIRGK+LKA Sbjct: 297 GIPRHGYPVLADGEQVGEVTTGTQSPTLKKNIGLALIKTQYAELGNEVEVEIRGKRLKAV 356 Query: 359 VVKTPFYKKSQ 369 + TPFYK+ + Sbjct: 357 IAATPFYKRDK 367 >C5D4A2.1 C5D4A2_9BACI Glycine cleavage system T protein Length = 364 Score = 450 bits (1158), Expect = e-124, Method: Compositional matrix adjust. Identities = 223/367 (60%), Positives = 275/367 (74%), Gaps = 5/367 (1%) Query: 4 LKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGS 63 LKRTP + +Y Y ++ IDFGGWELPVQF+ I +EHEAVR +AGLFDVSHMGEF+V G Sbjct: 2 LKRTPLFAVYERYG-AKTIDFGGWELPVQFSSIKEEHEAVRTRAGLFDVSHMGEFVVKGD 60 Query: 64 GSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNIDKD 123 S AFLQ M TNDVS+L DG QY+LM Y GG VDDLL+Y+ + Y+LVVNA+NI+KD Sbjct: 61 DSLAFLQKMMTNDVSKLTDGRVQYSLMCYEDGGTVDDLLIYKKADGHYLLVVNAANIEKD 120 Query: 124 FEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTSAPIAELAPFHFIERAVV 183 FEWL HL F V L N+S E LALQGPLAE +L ++T+ ++ + F F + + Sbjct: 121 FEWLHGHL---FGDVELVNISQEIAQLALQGPLAEQVLQKLTNTDLSAIKFFSFQDDINI 177 Query: 184 CGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFEAKL 243 GV+ L+SRTGYTGEDGFE+Y ++ A LW +L AG G+ P GLGARDTLRFEA L Sbjct: 178 NGVKALVSRTGYTGEDGFEIYCRREDAVALWESILEAGKEEGVLPCGLGARDTLRFEATL 237 Query: 244 PLYGQELSADITPLEAGVQFFVKLDK-AGFIGRDALLKQKESGLPRRLVGLEMIDRGIPR 302 PLYGQELS DITP+EAG+ F VK +K A FIG+D L KQKE G R+LVG+EMID+GIPR Sbjct: 238 PLYGQELSKDITPIEAGLGFAVKTNKDADFIGKDVLKKQKEEGTARKLVGIEMIDKGIPR 297 Query: 303 SHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAAVVKT 362 Y V+A+G +IG VTTGTQSPTLK+N+GLAL+ +TE TEV VEIRGK+LKA V+ T Sbjct: 298 HGYKVFANGEEIGFVTTGTQSPTLKKNIGLALIKTEFTEMDTEVEVEIRGKRLKAKVIAT 357 Query: 363 PFYKKSQ 369 PFYK+++ Sbjct: 358 PFYKRAK 364 >C0ZBW5.1 C0ZBW5_BREBN Aminomethyltransferase EC=2.1.2.10 Length = 367 Score = 449 bits (1155), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/370 (60%), Positives = 278/370 (75%), Gaps = 5/370 (1%) Query: 1 MEALKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMV 60 M LKRTP +D Y+ Y ++ IDFGGW+LPVQFT I +EHEAVR +AGLFDVSHMGE V Sbjct: 1 MSNLKRTPLFDSYAKYG-AKTIDFGGWDLPVQFTSIGQEHEAVRTKAGLFDVSHMGEVDV 59 Query: 61 SGSGSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNI 120 G + +LQ +TTNDVS+L G AQY+++ YP GG VDDLLVY+ ++ Y+LV+NA NI Sbjct: 60 KGENALTYLQRVTTNDVSKLAVGQAQYSVLCYPDGGTVDDLLVYKYADDHYLLVINAGNI 119 Query: 121 DKDFEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTSAPIAELAPFHFIER 180 DKD+ WL+EHL GVT+ N+S +T +A+QGPLAE+IL ++T+ ++++ F F Sbjct: 120 DKDYAWLEEHL---IPGVTIENISPQTAQIAIQGPLAESILQKLTTTDLSQIGFFRFERD 176 Query: 181 AVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFE 240 V G+ L+SRTGYTGEDGFE+Y D AA LW+ LL AG GL P GLGARDTLRFE Sbjct: 177 VQVSGIPGLVSRTGYTGEDGFEIYLDADRAAELWDILLDAGKEEGLLPCGLGARDTLRFE 236 Query: 241 AKLPLYGQELSADITPLEAGVQFFVKLDK-AGFIGRDALLKQKESGLPRRLVGLEMIDRG 299 AKLPLYGQELS DITP+EAG+ F VK+DK FIG++ L QKE+G PR+LVG+EMIDRG Sbjct: 237 AKLPLYGQELSKDITPIEAGIGFAVKVDKEVPFIGQEVLKAQKENGAPRKLVGIEMIDRG 296 Query: 300 IPRSHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAAV 359 IPR+HYPVY IGEVTTGTQSPTLK+N+GLAL+ + GT V VEIRGK+LKA V Sbjct: 297 IPRTHYPVYVGEELIGEVTTGTQSPTLKKNVGLALVKTEHAAIGTPVEVEIRGKRLKAEV 356 Query: 360 VKTPFYKKSQ 369 V PFYK+ + Sbjct: 357 VAAPFYKRPK 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,226,988,507 Number of extensions: 95939610 Number of successful extensions: 218918 Number of sequences better than 1.0e-05: 2319 Number of HSP's gapped: 210756 Number of HSP's successfully gapped: 2330 Length of query: 374 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 237 Effective length of database: 1,770,017,148 Effective search space: 419494064076 Effective search space used: 419494064076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)