BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0010.p (450 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KCW7.1 B3KCW7_9BACI Glycine dehydrogenase (Decarboxylating) EC... 605 e-171 B7GHD3.1 GCSPA_ANOFW Probable glycine dehydrogenase [decarboxyla... 509 e-142 A8FF40.1 GCSPA_BACP2 Probable glycine dehydrogenase [decarboxyla... 509 e-142 B4AL35.1 B4AL35_BACPU Glycine cleavage system P-protein, subunit... 508 e-142 C0ZBW6.1 GCSPA_BREBN Probable glycine dehydrogenase [decarboxyla... 503 e-140 >B3KCW7.1 B3KCW7_9BACI Glycine dehydrogenase (Decarboxylating) EC=1.4.4.2 Length = 449 Score = 605 bits (1561), Expect = e-171, Method: Compositional matrix adjust. Identities = 303/450 (67%), Positives = 355/450 (78%), Gaps = 1/450 (0%) Query: 1 MKHRYLPMTEQDRKEMMEAVGIQSVEELFADIPQSVRYHGTMPMSEALDEYTLLRHMKEL 60 MKHRYLPMT+QD+ +M+ AVG +++E+LFADIP+SVRY+G +PMS+ L E LL+HM+ L Sbjct: 1 MKHRYLPMTDQDQADMLAAVGAETIEDLFADIPKSVRYNGVLPMSKRLGEPELLKHMRRL 60 Query: 61 SDKNASFDTHASFLGAGLYDHHIPVVINHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 +D+NA FD + SFLGAGLYDHHIPVV+NHVISRSEFYTAYTPYQPEISQGELQAIFEFQS Sbjct: 61 ADRNADFDRYTSFLGAGLYDHHIPVVLNHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 Query: 121 YICELTGMKVANASMYDGXXXXXXXXXXXXXXTKRKKLVVSRTVHPEARQVLRTSAGAWG 180 YICELTGMKVANASMYDG TKRKK+V+SR VHPEAR+++ TSA Sbjct: 121 YICELTGMKVANASMYDGSTALAEAAALASGATKRKKIVISRAVHPEAREIVLTSAHGLD 180 Query: 181 LDVVEIDYKDGVTDGAKLAAAIDSDTAAVLVQSPNFFGAIEDLGAIEPLIHAVKGLLVVS 240 L+VVE+ +GVTD LAAA+ DTAAVL+QSPNFFG IED+ +IEPL+H K LLV+S Sbjct: 181 LEVVEVGIVNGVTDPDALAAAVTDDTAAVLLQSPNFFGCIEDVRSIEPLVHDQKALLVLS 240 Query: 241 ANPLALGILETPGKLGADIVVGDAQPLGIPASLGGPTCGFFAVAEPLMRRMPGRIVGQTV 300 NPL+LG+LE+PG LGADIVVGDAQPLGIPASLGGPTCGFFAV+EPLMRR+PGRIVGQTV Sbjct: 241 VNPLSLGLLESPGVLGADIVVGDAQPLGIPASLGGPTCGFFAVSEPLMRRIPGRIVGQTV 300 Query: 301 DRNGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYLSVMGKEGMREVGELNIR 360 D++GKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVY+S +GK GM+E LN+R Sbjct: 301 DKDGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYMSTLGKAGMQEAALLNVR 360 Query: 361 KSHYXXXXXXXXXXXXXXXXXXXXNEFVLKLPEGASVSGINAKLLKQXXXXXXXXXXXXP 420 KSHY NEFV+KLPEG +V +N+KLL+ P Sbjct: 361 KSHY-AADLVAKGSLSLPFSSPFFNEFVVKLPEGTNVKEVNSKLLEAGYIGGYDLGSSYP 419 Query: 421 ELAGHMLVAVTEKRSKTEIDQFRGALEGCI 450 EL G ML+AVTE+R+K EIDQF G LE + Sbjct: 420 ELHGCMLIAVTERRTKEEIDQFIGELEAIV 449 >B7GHD3.1 GCSPA_ANOFW Probable glycine dehydrogenase [decarboxylating] subunit 1 EC=1.4.4.2 Length = 448 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 248/440 (56%), Positives = 315/440 (71%), Gaps = 3/440 (0%) Query: 1 MKHRYLPMTEQDRKEMMEAVGIQSVEELFADIPQSVRYHGTMPMSEALDEYTLLRHMKEL 60 M HRYLPMTE+D+++M++ +G+QS++ELF+DIP+SVR+ G + A E L++ + L Sbjct: 1 MLHRYLPMTEEDKQQMLQTIGVQSIDELFSDIPESVRFQGEYNIKPAKSEPELMKELMAL 60 Query: 61 SDKNASFDTHASFLGAGLYDHHIPVVINHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 + KNA TH SFLGAG+YDH+IP +++HVISRSEFYTAYTPYQPEISQGELQAIFEFQ+ Sbjct: 61 AAKNADMKTHTSFLGAGVYDHYIPTIVDHVISRSEFYTAYTPYQPEISQGELQAIFEFQT 120 Query: 121 YICELTGMKVANASMYDGXXXXXXXXXXXXXXTKRKKLVVSRTVHPEARQVLRTSAGAWG 180 ICELTGM VAN+SMYDG TK+KK+++S VHPE R V++T A G Sbjct: 121 MICELTGMDVANSSMYDGGTALAEAALLSAAHTKKKKVLLSNAVHPEYRDVVKTYAKGPG 180 Query: 181 LDVVEIDYKDGVTDGAKLAAAIDSDTAAVLVQSPNFFGAIEDLGAIEPLIHAVKGLLVVS 240 L+VVEI YK+GVTD A L A ++ D A V+VQ PNFFG IE L IEP+ HA KG+ VV+ Sbjct: 181 LEVVEIPYKNGVTDLAALQAEMNEDVACVIVQYPNFFGQIEPLKDIEPIAHAHKGMFVVA 240 Query: 241 ANPLALGILETPGKLGADIVVGDAQPLGIPASLGGPTCGFFAVAEPLMRRMPGRIVGQTV 300 +NPLALG+L PG+ GADIVVGDAQP GIPA GGP CG+FAV LMR++PGR+VGQT Sbjct: 241 SNPLALGVLTPPGQFGADIVVGDAQPFGIPAQFGGPHCGYFAVKSALMRKIPGRLVGQTT 300 Query: 301 DRNGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYLSVMGKEGMREVGELNIR 360 D G+RGFVLTLQAREQHIRR+KATSNICSNQAL AL ASV ++ +GK G++E+ +NI+ Sbjct: 301 DEEGRRGFVLTLQAREQHIRRDKATSNICSNQALNALAASVAMTALGKNGVKEMATMNIQ 360 Query: 361 KSHYXXXXXXXXXXXXXXXXXXXXNEFVLKLPEGASVSGINAKLLKQXXXXXXXXXXXXP 420 K+HY NEFV+++ ++ +N KLL++ P Sbjct: 361 KAHY-AKEAFVSRGFHVVFEGPFFNEFVIRM--NKPIAEVNKKLLEKGIIGGYDLGRNYP 417 Query: 421 ELAGHMLVAVTEKRSKTEID 440 EL ML+AVTE R+K EID Sbjct: 418 ELQNCMLIAVTELRTKEEID 437 >A8FF40.1 GCSPA_BACP2 Probable glycine dehydrogenase [decarboxylating] subunit 1 EC=1.4.4.2 Length = 448 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 255/442 (57%), Positives = 315/442 (71%), Gaps = 3/442 (0%) Query: 1 MKHRYLPMTEQDRKEMMEAVGIQSVEELFADIPQSVRYHGTMPMSEALDEYTLLRHMKEL 60 MKHRYLP TEQD+KEM++ +G+QS++ELF+DIP+ VR+ G + A E L+R + +L Sbjct: 1 MKHRYLPQTEQDQKEMLDVIGVQSIDELFSDIPEKVRFKGAYNIKPAASETELVRELSQL 60 Query: 61 SDKNASFDTHASFLGAGLYDHHIPVVINHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 + KN ++ASFLGAG+YDH+ PV+++HVISRSEFYTAYTPYQPEISQGELQAIFEFQ+ Sbjct: 61 AAKNKDTVSYASFLGAGVYDHYQPVIVDHVISRSEFYTAYTPYQPEISQGELQAIFEFQT 120 Query: 121 YICELTGMKVANASMYDGXXXXXXXXXXXXXXTKRKKLVVSRTVHPEARQVLRTSAGAWG 180 I ELTGM +AN+SMYDG TK+KK+VVS TVHPEAR VL+T A Sbjct: 121 MIAELTGMDLANSSMYDGGTALAEAAMLAAGHTKKKKVVVSETVHPEARAVLKTYAKGQH 180 Query: 181 LDVVEIDYKDGVTDGAKLAAAIDSDTAAVLVQSPNFFGAIEDLGAIEPLIHAVKGLLVVS 240 ++VVE+ K G TD A L A+ +TAAVLVQ PNFFG +E L IEP+ H K L VVS Sbjct: 181 IEVVEVPAKKGQTDLAALEKAVCDETAAVLVQYPNFFGVVEPLKDIEPIAHKGKSLFVVS 240 Query: 241 ANPLALGILETPGKLGADIVVGDAQPLGIPASLGGPTCGFFAVAEPLMRRMPGRIVGQTV 300 +NPLALG+L PGKLGADIVVGDAQP GIPA+ GGP CG+FAV + LMR++PGR+VGQT Sbjct: 241 SNPLALGLLTPPGKLGADIVVGDAQPFGIPAAFGGPHCGYFAVTKKLMRKVPGRLVGQTE 300 Query: 301 DRNGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYLSVMGKEGMREVGELNIR 360 D NG RGFVLTLQAREQHIRR+KATSNICSNQAL AL ASV ++ +GK G++++ N++ Sbjct: 301 DENGVRGFVLTLQAREQHIRRDKATSNICSNQALNALAASVAMTALGKTGIKDIAYQNVQ 360 Query: 361 KSHYXXXXXXXXXXXXXXXXXXXXNEFVLKLPEGASVSGINAKLLKQXXXXXXXXXXXXP 420 K+HY NEFV+KL E V +N +LL++ P Sbjct: 361 KAHY-AKTQAETYGLLADVEGTHFNEFVIKLQE--PVQEVNKRLLEKGIIGGYDLGRDYP 417 Query: 421 ELAGHMLVAVTEKRSKTEIDQF 442 EL HMLVAVTE R+K EID F Sbjct: 418 ELQHHMLVAVTELRTKEEIDTF 439 >B4AL35.1 B4AL35_BACPU Glycine cleavage system P-protein, subunit 1 EC=1.4.4.2 Length = 448 Score = 508 bits (1309), Expect = e-142, Method: Compositional matrix adjust. Identities = 256/442 (57%), Positives = 313/442 (70%), Gaps = 3/442 (0%) Query: 1 MKHRYLPMTEQDRKEMMEAVGIQSVEELFADIPQSVRYHGTMPMSEALDEYTLLRHMKEL 60 MKHRYLP TEQD+KEM++ +G+QS++ELF+DIP+ VR+ G + A E L+R + L Sbjct: 1 MKHRYLPQTEQDQKEMLDVIGVQSIDELFSDIPEKVRFKGAYNIKAAASETELVRELSRL 60 Query: 61 SDKNASFDTHASFLGAGLYDHHIPVVINHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 + KN ++ASFLGAG+YDH+ PV+++HVISRSEFYTAYTPYQPEISQGELQAIFEFQ+ Sbjct: 61 AAKNKDTVSYASFLGAGVYDHYQPVIVDHVISRSEFYTAYTPYQPEISQGELQAIFEFQT 120 Query: 121 YICELTGMKVANASMYDGXXXXXXXXXXXXXXTKRKKLVVSRTVHPEARQVLRTSAGAWG 180 I ELTGM +AN+SMYDG TK+KK+VVS TVHPEAR VL+T A Sbjct: 121 MIAELTGMDLANSSMYDGGTALAEAAMLAAGHTKKKKVVVSETVHPEARAVLKTYAKGQH 180 Query: 181 LDVVEIDYKDGVTDGAKLAAAIDSDTAAVLVQSPNFFGAIEDLGAIEPLIHAVKGLLVVS 240 ++VVE+ K G TD A L A+ DTAAVLVQ PNFFG +E L IEP+ H K L VVS Sbjct: 181 IEVVEVPAKKGQTDLAALEKAVCEDTAAVLVQYPNFFGVVEPLKDIEPIAHKGKSLFVVS 240 Query: 241 ANPLALGILETPGKLGADIVVGDAQPLGIPASLGGPTCGFFAVAEPLMRRMPGRIVGQTV 300 +NPLALG+L PGKLGADIVVGDAQP GIPA+ GGP CG+FAV + LMR++PGR+VGQT Sbjct: 241 SNPLALGLLTPPGKLGADIVVGDAQPFGIPAAFGGPHCGYFAVTKKLMRKVPGRLVGQTE 300 Query: 301 DRNGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYLSVMGKEGMREVGELNIR 360 D NG RGFVLTLQAREQHIRR+KATSNICSNQAL AL ASV ++ +GK G++++ N++ Sbjct: 301 DENGVRGFVLTLQAREQHIRRDKATSNICSNQALNALAASVAMTALGKTGIKDIAYQNVQ 360 Query: 361 KSHYXXXXXXXXXXXXXXXXXXXXNEFVLKLPEGASVSGINAKLLKQXXXXXXXXXXXXP 420 K+HY NEFV+KL E V N +LL++ P Sbjct: 361 KAHY-AKTQAKAYGLLADVEGAHFNEFVIKLQE--PVKEANKRLLEKGIIGGYDLGRDFP 417 Query: 421 ELAGHMLVAVTEKRSKTEIDQF 442 EL HMLVAVTE R+K EID F Sbjct: 418 ELQHHMLVAVTELRTKEEIDTF 439 >C0ZBW6.1 GCSPA_BREBN Probable glycine dehydrogenase [decarboxylating] subunit 1 EC=1.4.4.2 Length = 450 Score = 503 bits (1296), Expect = e-140, Method: Compositional matrix adjust. Identities = 252/448 (56%), Positives = 317/448 (70%), Gaps = 4/448 (0%) Query: 1 MKHRYLPMTEQDRKEMMEAVGIQSVEELFADIPQSVRYHGTMPMSEALDEYTLLRHMKEL 60 MK+RYLP T+QD++EM+E +GI SVEELFADIP+ VR+ G + + EAL E L+++ L Sbjct: 1 MKYRYLPQTDQDKREMLETLGISSVEELFADIPEEVRFKGALNIPEALSEPDLVKYFTHL 60 Query: 61 SDKNASFDTHASFLGAGLYDHHIPVVINHVISRSEFYTAYTPYQPEISQGELQAIFEFQS 120 ++KN +F TH +FLGAG+Y H+ P +NH++ R EF+TAYTPYQPEISQGELQAIFEFQ+ Sbjct: 61 ANKNVNFSTHVNFLGAGVYQHYTPSTVNHMLLRGEFFTAYTPYQPEISQGELQAIFEFQT 120 Query: 121 YICELTGMKVANASMYDGXXXXXXXXXXXXXXTKRKKLVVSRTVHPEARQVLRTSAGAWG 180 +CELTGM+VAN+SMYDG T +K+++VSR VHPEAR VL+T A Sbjct: 121 MVCELTGMEVANSSMYDGATSLAEAAMMAAGHTGKKRVIVSRAVHPEARGVLKTYAYGQN 180 Query: 181 LDVVEIDYK-DGVTDGAKLAAAIDSDTAAVLVQSPNFFGAIEDLGAIEPLIHAVKGLLVV 239 +++VE+ DGVTD A L A +D +TAA++VQ PNFFG +EDLGAIE + H LL+ Sbjct: 181 VELVEVGINSDGVTDTAALEALVDENTAAIIVQYPNFFGNVEDLGAIETIAHGKGALLIT 240 Query: 240 SANPLALGILETPGKLGADIVVGDAQPLGIPASLGGPTCGFFAVAEPLMRRMPGRIVGQT 299 S+NPLALG+LE PGKLGADIVVGD QP GIPAS GGP CG+FA LMR+MPGRIVGQT Sbjct: 241 SSNPLALGVLEAPGKLGADIVVGDMQPFGIPASFGGPHCGYFATTTKLMRKMPGRIVGQT 300 Query: 300 VDRNGKRGFVLTLQAREQHIRREKATSNICSNQALLALCASVYLSVMGKEGMREVGELNI 359 D NGKRGFVLTLQAREQHIRREKATSNICSNQALLAL AS+ ++ +GK+G++E+ +N+ Sbjct: 301 KDENGKRGFVLTLQAREQHIRREKATSNICSNQALLALAASIAMTALGKQGVQEMAMMNL 360 Query: 360 RKSHYXXXXXXXXXXXXXXXXXXXXNEFVLKLPEGASVSGINAKLLKQXXXXXXXXXXXX 419 +K+HY NEFV+KL + V+ +N LL Sbjct: 361 QKAHY-AKNALQAKGLEIVFTSPFFNEFVVKLNK--PVAEVNKGLLAAGIIGGYDLGLDY 417 Query: 420 PELAGHMLVAVTEKRSKTEIDQFRGALE 447 PE A H L+AVTE R+K EID LE Sbjct: 418 PEFANHTLLAVTELRTKEEIDILAAELE 445 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,278,109,346 Number of extensions: 88702150 Number of successful extensions: 207676 Number of sequences better than 1.0e-05: 1551 Number of HSP's gapped: 204715 Number of HSP's successfully gapped: 1556 Length of query: 450 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 311 Effective length of database: 1,751,175,118 Effective search space: 544615461698 Effective search space used: 544615461698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)