BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0009.p (486 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9K936.1 GCSPB_BACHD Probable glycine dehydrogenase [decarboxyla... 693 0.0 B4BLL9.1 B4BLL9_9BACI Glycine dehydrogenase (Decarboxylating) EC... 681 0.0 A6CRF3.1 A6CRF3_9BACI Glycine dehydrogenase subunit 2 EC=1.4.4.2 679 0.0 A7GSN6.1 GCSPB_BACCN Probable glycine dehydrogenase [decarboxyla... 677 0.0 Q5KX78.1 Q5KX78_GEOKA Glycine cleavage system P-protein, glycine... 677 0.0 >Q9K936.1 GCSPB_BACHD Probable glycine dehydrogenase [decarboxylating] subunit 2 EC=1.4.4.2 Length = 488 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/482 (70%), Positives = 391/482 (81%), Gaps = 3/482 (0%) Query: 1 MKPEQSLIFELSRPGRSAYSLPQCDVPQEESIDALIPAGLLRSEPAVLPEVSEVDVIRHY 60 M +Q+LIFELS+PGR +SLP+ DV E+ ++ LIPA LR E LPEVSE+ ++RHY Sbjct: 2 MNKDQALIFELSKPGRVGHSLPELDV-LEQPVETLIPAEFLREEAPELPEVSELQLMRHY 60 Query: 61 TALSRRNFGVDNGFYPLGSCTMKYNPKINEDVARFPGLAKIHPYQPEESIQGALELMHTL 120 TALS+RN GVD+GFYPLGSCTMKYNPKINEDVAR+PG A IHPYQPE +QGAL LM+ L Sbjct: 61 TALSKRNHGVDSGFYPLGSCTMKYNPKINEDVARYPGFANIHPYQPEAQVQGALRLMYEL 120 Query: 121 QKDLAALTGMDAVSLQPAAGAHGEWTGLMMIRAYHESRGET-RSKVIVPDSSHGTNPASA 179 Q LA +TGMD V+LQPAAGA GEWTGLM+IRAYHE+ G+T R+KVIVPDS+HGTNPASA Sbjct: 121 QTALAEITGMDEVTLQPAAGAQGEWTGLMLIRAYHEANGDTNRTKVIVPDSAHGTNPASA 180 Query: 180 SAAGLETVTIPSNDKGMVDLAALKAAVGSDTAALMLTNPSTLGLFETQIVEIARIVHEAG 239 + AG E+VT+ +++ G+VDL L+ VG DTAALMLTNP+TLGLFE IVE+A I+HEAG Sbjct: 181 TVAGFESVTVRTDEDGLVDLDHLREVVGEDTAALMLTNPNTLGLFEAHIVEMAAIIHEAG 240 Query: 240 GLLYYDGANSNAIMGITRPGDMGFDVVHLNLHKTMSTPHXXXXXXXXXXXXXXXLIPFLP 299 G LYYDGANSNAI+GI RPGDMGFDVVHLNLHKT + PH LIP+LP Sbjct: 241 GKLYYDGANSNAILGIARPGDMGFDVVHLNLHKTFTGPHGGGGPGSGPVGVKKELIPYLP 300 Query: 300 QPTVVQNEDSSYSLNYGGPESIGRVKAFYGNFGILVRAYAYIRTYGPDGLREVSENAVLN 359 +P VV++ DS Y L+Y P SIGRVK +YGNFGI VRAY YIRT GP+GLR VSE AVLN Sbjct: 301 KPVVVKDGDS-YRLDYDRPHSIGRVKPYYGNFGINVRAYTYIRTMGPEGLRTVSEYAVLN 359 Query: 360 ANYMMHRLAPYFEIPYPGVCKHEFVMSGRNLKQYGVRTLDVAKRLLDFGYHPPTVYFPLT 419 ANYMM RLAPYF++PY CKHEFV+SGR K+ GVRTLD+AKRLLDFGYHPPT+YFPL Sbjct: 360 ANYMMRRLAPYFDLPYDQHCKHEFVLSGRQQKKLGVRTLDIAKRLLDFGYHPPTIYFPLN 419 Query: 420 VEECMMIEPTETESKETLDGFIETMIQIVKEAQETPEIVINAPHTTEISRLDETQAARKP 479 VEEC+MIEPTETESKETLD FIE MIQI KEA+ETPE+V APH T I RLDET AARKP Sbjct: 420 VEECLMIEPTETESKETLDEFIEAMIQIAKEAEETPEVVQEAPHHTVIGRLDETTAARKP 479 Query: 480 VL 481 +L Sbjct: 480 IL 481 >B4BLL9.1 B4BLL9_9BACI Glycine dehydrogenase (Decarboxylating) EC=1.4.4.2 Length = 487 Score = 681 bits (1756), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/482 (69%), Positives = 386/482 (80%), Gaps = 3/482 (0%) Query: 1 MKPEQSLIFELSRPGRSAYSLPQCDVPQEESIDALIPAGLLRSEPAVLPEVSEVDVIRHY 60 M +Q LIFE S+PGR AYSLP DVP + + L+PA LR+E LPEVSE+D++RHY Sbjct: 1 MHNDQPLIFERSKPGRIAYSLPTLDVPAVD-VSELVPADYLRTEEPELPEVSELDLMRHY 59 Query: 61 TALSRRNFGVDNGFYPLGSCTMKYNPKINEDVARFPGLAKIHPYQPEESIQGALELMHTL 120 TALS+RN GVD+GFYPLGSCTMKYNPKINE+VAR G A IHP QPEE++QGALELM+ L Sbjct: 60 TALSKRNHGVDSGFYPLGSCTMKYNPKINENVARLAGFAHIHPLQPEETVQGALELMYDL 119 Query: 121 QKDLAALTGMDAVSLQPAAGAHGEWTGLMMIRAYHESRGE-TRSKVIVPDSSHGTNPASA 179 Q+ L +TGMDAV+LQPAAGAHGEWTGLMMIRAYHE+ G+ R+KVIVPDS+HGTNPASA Sbjct: 120 QEHLKEITGMDAVTLQPAAGAHGEWTGLMMIRAYHEANGDFGRTKVIVPDSAHGTNPASA 179 Query: 180 SAAGLETVTIPSNDKGMVDLAALKAAVGSDTAALMLTNPSTLGLFETQIVEIARIVHEAG 239 + AG ETVT+ S G+VDL LK VG DTAALMLTNP+TLGLFE QIVE+A IVHEAG Sbjct: 180 TVAGFETVTVRSTADGLVDLEDLKRVVGPDTAALMLTNPNTLGLFEEQIVEMAEIVHEAG 239 Query: 240 GLLYYDGANSNAIMGITRPGDMGFDVVHLNLHKTMSTPHXXXXXXXXXXXXXXXLIPFLP 299 G LYYDGAN NAI+G RPGDMGFDVVHLNLHKT + PH LIPFLP Sbjct: 240 GKLYYDGANLNAILGKARPGDMGFDVVHLNLHKTFTGPHGGGGPGSGPVGVKADLIPFLP 299 Query: 300 QPTVVQNEDSSYSLNYGGPESIGRVKAFYGNFGILVRAYAYIRTYGPDGLREVSENAVLN 359 +P + + E+ Y L+ P+SIGRVK FYGNFGI VRAY YIR+ GPDGL+ VSE AVLN Sbjct: 300 KPVIAKGENGYY-LDDDRPQSIGRVKPFYGNFGINVRAYTYIRSMGPDGLKAVSEYAVLN 358 Query: 360 ANYMMHRLAPYFEIPYPGVCKHEFVMSGRNLKQYGVRTLDVAKRLLDFGYHPPTVYFPLT 419 ANYMM RLA Y+++PY CKHEFV+SG+ K+ GVRTLD+AKRLLDFG+HPPT+YFPL Sbjct: 359 ANYMMRRLADYYDLPYNRHCKHEFVLSGKRQKKLGVRTLDIAKRLLDFGFHPPTIYFPLI 418 Query: 420 VEECMMIEPTETESKETLDGFIETMIQIVKEAQETPEIVINAPHTTEISRLDETQAARKP 479 VEECMMIEPTETESKETLD FI+ MIQI KEA+ETPEIV APHTT + RLDET AARKP Sbjct: 419 VEECMMIEPTETESKETLDAFIDAMIQIAKEAEETPEIVQEAPHTTVVKRLDETTAARKP 478 Query: 480 VL 481 +L Sbjct: 479 IL 480 >A6CRF3.1 A6CRF3_9BACI Glycine dehydrogenase subunit 2 EC=1.4.4.2 Length = 487 Score = 679 bits (1752), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/482 (68%), Positives = 385/482 (79%), Gaps = 3/482 (0%) Query: 1 MKPEQSLIFELSRPGRSAYSLPQCDVPQEESIDALIPAGLLRSEPAVLPEVSEVDVIRHY 60 +K +Q LIFELS+ GR+ YSLP+ DVPQ + + LIPA R E LPEVSE+D++RHY Sbjct: 2 LKNDQPLIFELSKEGRTGYSLPEMDVPQVD-LSELIPAEYTREEAPELPEVSELDIMRHY 60 Query: 61 TALSRRNFGVDNGFYPLGSCTMKYNPKINEDVARFPGLAKIHPYQPEESIQGALELMHTL 120 TALS+RN GVD+GFYPLGSCTMKYNPKINE+VARF G A +HP Q + +QGALELM L Sbjct: 61 TALSKRNHGVDSGFYPLGSCTMKYNPKINENVARFNGFAHLHPLQETKGVQGALELMFDL 120 Query: 121 QKDLAALTGMDAVSLQPAAGAHGEWTGLMMIRAYHESRGET-RSKVIVPDSSHGTNPASA 179 Q+ L +TGMD V+LQPAAGAHGEWTGLMMIRAYHE+ G+T R+KVIVPDS+HGTNPASA Sbjct: 121 QEHLKEITGMDEVTLQPAAGAHGEWTGLMMIRAYHEANGDTNRTKVIVPDSAHGTNPASA 180 Query: 180 SAAGLETVTIPSNDKGMVDLAALKAAVGSDTAALMLTNPSTLGLFETQIVEIARIVHEAG 239 + AG ETVT+ S++ G+VD+ LK VG DTAALMLTNP+TLGLFE I+E+A IVHEAG Sbjct: 181 TVAGFETVTVKSDENGLVDMEDLKRVVGEDTAALMLTNPNTLGLFEENILEMASIVHEAG 240 Query: 240 GLLYYDGANSNAIMGITRPGDMGFDVVHLNLHKTMSTPHXXXXXXXXXXXXXXXLIPFLP 299 G LYYDGAN NA+M RPGDMGFDVVHLNLHKT + PH LIP+LP Sbjct: 241 GKLYYDGANLNAVMSKARPGDMGFDVVHLNLHKTFTGPHGGGGPGSGPVGVKADLIPYLP 300 Query: 300 QPTVVQNEDSSYSLNYGGPESIGRVKAFYGNFGILVRAYAYIRTYGPDGLREVSENAVLN 359 +P +V+ +D Y Y P+SIGRVK +YGNFGI VRAY YIRT GPDGL+ V+ENAV+N Sbjct: 301 KPVLVKRDDE-YVFEYDRPQSIGRVKPYYGNFGINVRAYTYIRTMGPDGLKAVTENAVIN 359 Query: 360 ANYMMHRLAPYFEIPYPGVCKHEFVMSGRNLKQYGVRTLDVAKRLLDFGYHPPTVYFPLT 419 ANYMM RLAP+F++PY CKHEFV+SG+ K+ GVRTLD+AKRLLDFGYHPPT+YFPL Sbjct: 360 ANYMMRRLAPHFDLPYDRHCKHEFVISGKRQKKLGVRTLDIAKRLLDFGYHPPTIYFPLN 419 Query: 420 VEECMMIEPTETESKETLDGFIETMIQIVKEAQETPEIVINAPHTTEISRLDETQAARKP 479 VEECMMIEPTETESKET+D FI+ MIQI KEA+E PEIV APHTT I RLDET AARKP Sbjct: 420 VEECMMIEPTETESKETIDAFIDAMIQIAKEAEENPEIVQEAPHTTVIKRLDETLAARKP 479 Query: 480 VL 481 VL Sbjct: 480 VL 481 >A7GSN6.1 GCSPB_BACCN Probable glycine dehydrogenase [decarboxylating] subunit 2 EC=1.4.4.2 Length = 491 Score = 677 bits (1747), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/479 (67%), Positives = 388/479 (81%), Gaps = 3/479 (0%) Query: 4 EQSLIFELSRPGRSAYSLPQCDVPQEESIDALIPAGLLRSEPAVLPEVSEVDVIRHYTAL 63 +Q+LIFE+S+ GR YSLPQ DV +E ++ + + +R+E A LPEVSE+D++RHYTAL Sbjct: 5 DQALIFEMSKEGRVGYSLPQLDV-EEVKLEDVFESNYIRAEDAELPEVSELDIMRHYTAL 63 Query: 64 SRRNFGVDNGFYPLGSCTMKYNPKINEDVARFPGLAKIHPYQPEESIQGALELMHTLQKD 123 S RN GVD+GFYPLGSCTMKYNPKINE+VARFPG A IHP Q E+++QGA+ELM+ LQ+ Sbjct: 64 SNRNHGVDSGFYPLGSCTMKYNPKINENVARFPGFANIHPLQDEKTVQGAMELMYDLQEH 123 Query: 124 LAALTGMDAVSLQPAAGAHGEWTGLMMIRAYHESRGE-TRSKVIVPDSSHGTNPASASAA 182 L +TGMDAV+LQPAAGAHGEWTGLM+IRAYHE+ G+ R+KVIVPDS+HGTNPASA+ A Sbjct: 124 LVEITGMDAVTLQPAAGAHGEWTGLMLIRAYHEANGDHNRTKVIVPDSAHGTNPASATVA 183 Query: 183 GLETVTIPSNDKGMVDLAALKAAVGSDTAALMLTNPSTLGLFETQIVEIARIVHEAGGLL 242 G ET+T+ SN+ G+VDL LK V +TAALMLTNP+TLGLFE I+E+A IVH AGG L Sbjct: 184 GFETITVKSNEHGLVDLEDLKRVVNEETAALMLTNPNTLGLFEENILEMAEIVHNAGGKL 243 Query: 243 YYDGANSNAIMGITRPGDMGFDVVHLNLHKTMSTPHXXXXXXXXXXXXXXXLIPFLPQPT 302 YYDGAN NA++ RPGDMGFDVVHLNLHKT + PH LIPFLP+P Sbjct: 244 YYDGANLNAVLSQARPGDMGFDVVHLNLHKTFTGPHGGGGPGSGPVGVKEDLIPFLPKP- 302 Query: 303 VVQNEDSSYSLNYGGPESIGRVKAFYGNFGILVRAYAYIRTYGPDGLREVSENAVLNANY 362 +++ ++ Y NY P++IGRVK FYGNFGI VRAY YIR+ GPDGLR V+ENAVLNANY Sbjct: 303 ILEKTENGYHFNYDRPQAIGRVKPFYGNFGINVRAYTYIRSMGPDGLRAVTENAVLNANY 362 Query: 363 MMHRLAPYFEIPYPGVCKHEFVMSGRNLKQYGVRTLDVAKRLLDFGYHPPTVYFPLTVEE 422 MM RLAPY+++P+ CKHEFV+SGR K+ GVRTLD+AKRLLDFGYHPPT+YFPL VEE Sbjct: 363 MMRRLAPYYDLPFDRHCKHEFVLSGRCQKKLGVRTLDIAKRLLDFGYHPPTIYFPLNVEE 422 Query: 423 CMMIEPTETESKETLDGFIETMIQIVKEAQETPEIVINAPHTTEISRLDETQAARKPVL 481 C+MIEPTETESKETLDGFI+ MIQI KEA+E PEIV APHTT I+RLDET AARKP+L Sbjct: 423 CIMIEPTETESKETLDGFIDKMIQIAKEAKENPEIVQEAPHTTVITRLDETMAARKPIL 481 >Q5KX78.1 Q5KX78_GEOKA Glycine cleavage system P-protein, glycine dehydrogenase [decarboxylating] subunit 2 (Glycine decarboxylase) EC=1.4.4.2 Length = 498 Score = 677 bits (1746), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/482 (69%), Positives = 385/482 (79%), Gaps = 3/482 (0%) Query: 1 MKPEQSLIFELSRPGRSAYSLPQCDVPQEESIDALIPAGLLRSEPAVLPEVSEVDVIRHY 60 M +Q LIFE S+PGR AYSLP DVP + + L+PA LR+E LPEVSE+D++RHY Sbjct: 9 MHNDQPLIFERSKPGRIAYSLPALDVPAVD-VSELVPADYLRTEEPELPEVSELDLMRHY 67 Query: 61 TALSRRNFGVDNGFYPLGSCTMKYNPKINEDVARFPGLAKIHPYQPEESIQGALELMHTL 120 TALS+RN GVD+GFYPLGSCTMKYNPKINE+VAR G A IHP QPE+++QGALELM+ L Sbjct: 68 TALSKRNHGVDSGFYPLGSCTMKYNPKINENVARLAGFAHIHPLQPEKTVQGALELMYDL 127 Query: 121 QKDLAALTGMDAVSLQPAAGAHGEWTGLMMIRAYHESRGE-TRSKVIVPDSSHGTNPASA 179 Q+ L +TGMDAV+LQPAAGAHGEWTGLMMIRAYHE+ G+ R+KVIVPDS+HGTNPASA Sbjct: 128 QEHLKEITGMDAVTLQPAAGAHGEWTGLMMIRAYHEANGDFNRTKVIVPDSAHGTNPASA 187 Query: 180 SAAGLETVTIPSNDKGMVDLAALKAAVGSDTAALMLTNPSTLGLFETQIVEIARIVHEAG 239 + AG ETVT+ S G+VDL LK VG DTAALMLTNP+TLGLFE QIVE+A IVH AG Sbjct: 188 TVAGFETVTVKSTADGLVDLEDLKRVVGPDTAALMLTNPNTLGLFEEQIVEVAEIVHAAG 247 Query: 240 GLLYYDGANSNAIMGITRPGDMGFDVVHLNLHKTMSTPHXXXXXXXXXXXXXXXLIPFLP 299 G LYYDGAN NAI+G RPGDMGFDVVHLNLHKT + PH L+PFLP Sbjct: 248 GKLYYDGANLNAILGKARPGDMGFDVVHLNLHKTFTGPHGGGGPGSGPVGVKADLVPFLP 307 Query: 300 QPTVVQNEDSSYSLNYGGPESIGRVKAFYGNFGILVRAYAYIRTYGPDGLREVSENAVLN 359 +P V++ ++ Y L+ P+SIGRVK FYGNFGI VRAY YIR+ GPDGL+ VSE AVLN Sbjct: 308 KP-VIEKGENGYYLDDDRPQSIGRVKPFYGNFGINVRAYTYIRSMGPDGLKAVSEYAVLN 366 Query: 360 ANYMMHRLAPYFEIPYPGVCKHEFVMSGRNLKQYGVRTLDVAKRLLDFGYHPPTVYFPLT 419 ANYMM RLA Y+++PY CKHEFV+SGR K+ GVRTLD+AKRLLDFG+HPPT+YFPL Sbjct: 367 ANYMMRRLADYYDLPYDRHCKHEFVLSGRRQKKLGVRTLDIAKRLLDFGFHPPTIYFPLI 426 Query: 420 VEECMMIEPTETESKETLDGFIETMIQIVKEAQETPEIVINAPHTTEISRLDETQAARKP 479 VEECMMIEPTETESKETLD FI+ MIQI KEA+E PEIV APHTT + RLDET AARKP Sbjct: 427 VEECMMIEPTETESKETLDAFIDAMIQIAKEAEENPEIVQEAPHTTVVKRLDETTAARKP 486 Query: 480 VL 481 +L Sbjct: 487 IL 488 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,877,116,016 Number of extensions: 118700399 Number of successful extensions: 288491 Number of sequences better than 1.0e-05: 1459 Number of HSP's gapped: 282947 Number of HSP's successfully gapped: 1463 Length of query: 486 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 346 Effective length of database: 1,741,754,103 Effective search space: 602646919638 Effective search space used: 602646919638 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)