BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0004.p (387 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7C2.1 B3K7C2_9BACI ABC-2 type transporter 216 2e-54 C0GCV7.1 C0GCV7_9FIRM ABC-2 type transporter 70 2e-10 A7Z0X4.1 A7Z0X4_BACA2 YfiM 68 1e-09 C1FSH3.1 C1FSH3_CLOBJ Multidrug ABC transporter, permease 64 3e-08 B0KBI8.1 B0KBI8_THEP3 ABC-2 type transporter 64 3e-08 >B3K7C2.1 B3K7C2_9BACI ABC-2 type transporter Length = 393 Score = 216 bits (551), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 122/357 (34%), Positives = 190/357 (53%), Gaps = 6/357 (1%) Query: 36 GASLSGVVGVKSATKIEPVRVAVV--NPAGDGQSAMIAAFLKLPDIQKVIVPQDAENRQA 93 G +LSG G K VRV +V AG + A LP IQ ++ P+ +R+ Sbjct: 36 GNALSGWFGNDQEFKQPSVRVGLVLDAEAGGQLPGSMQALTGLPQIQDLLEPEVVSSREE 95 Query: 94 AESGLRTGKYGYAVIVPDGFDSEVQQGGKAQLEFILGKNHTDNMTAGTAFDNFLSTVNYK 153 AE LR G+ YAV+VP FD + QG +LE + G++ N+ A T F F++ N+K Sbjct: 96 AEEKLRRGEVDYAVVVPASFDERMSQGADVKLELLPGRDRELNLVADTVFKTFIADANHK 155 Query: 154 XXXXXXX---XXXXXXXXXP-SSEDREAVKLGDLNNGGRTYTASQXXXXXXXXXXXXXXG 209 P + D V +G L+ G TY+A+Q G Sbjct: 156 QAEVVVMGGDQVLAAQAAAPVDASDGPNVTIGQLSEKGATYSAAQYYAASMLIMFLLYSG 215 Query: 210 MTVTNSLFSEKDNHTLHRINSMPVKASELFIGKMLGVGIVSIIQCLAIILLSDWLFGVYW 269 + ++SL E+++ TL+R+ S PV +F GK++G ++++ Q AI+L S WL+GV W Sbjct: 216 LMASSSLLGERESRTLYRLQSAPVNPGTVFAGKIIGCSLITLAQAAAIVLGSMWLYGVKW 275 Query: 270 GNRPGXXXXXXXXMIIASMTMSIVVSMFSKTAAGARSIISLITVGMTFISGGMAPLPDSW 329 G P + ++SMT++ +++ S TAAGAR ++ I + MTF+SGG PLP + Sbjct: 276 GPHPLLLIIVCVLITLSSMTIATFITLVSSTAAGARGLMQAIIIAMTFVSGGFMPLPVEF 335 Query: 330 VNTLGAFTINHWVLQAIIRMMLHSGLPQLTPNLLMISVICLVMLCGAILSYWKVGYH 386 LGAFT+NHW +Q+++RMML S + + + M++ I + A+++Y KVGYH Sbjct: 336 FQKLGAFTVNHWAMQSMLRMMLDSDVHLIVTCVGMLAAITAALSAAAMITYRKVGYH 392 >C0GCV7.1 C0GCV7_9FIRM ABC-2 type transporter Length = 391 Score = 70.5 bits (171), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 65/275 (23%), Positives = 109/275 (39%), Gaps = 21/275 (7%) Query: 94 AESGLRTGKYGYAVIVPDGFDSEVQQGGKAQLEFILGKNH-TDNMTAGTAFDNFLSTVNY 152 AE + G AVIVP GF G +L + + M A + NF +++ Sbjct: 91 AEKHVNAGNLAAAVIVPAGFSELFMAGEPVELTLLTDQTRFISPMVAESLLQNFAESLSA 150 Query: 153 KXXXXXXXXXXXXXXXXPSSEDREAVKLG----------DLNNGGRTYTASQXXXXXXXX 202 + EAV L +L+ T+ Q Sbjct: 151 GQLAVVQAVSGGYADGQELASLGEAVALDLQATMPQIKEELSQRDALITSFQYYTAAMAV 210 Query: 203 XXXXXXGMTVTNSLFSEKDNHTLHRINSMPVKASELFIGKMLGVGIVSIIQCLAIILLSD 262 GM ++ +E+ NHT HR+ + P+ S+ GK G+ V Q L ++L + Sbjct: 211 MFMLFAGMNGLQAIVAERRNHTFHRLAASPLSRSQFVWGKFFGIVAVCFAQFLVLMLGTR 270 Query: 263 WLFGVYWGNRPGXXXXXXXXMIIASMTMSIVVSMFSKTAAGARSIISLITVGMTFIS--- 319 +L+GV WG+RP +A +S+ M S ++++++ +G+ S Sbjct: 271 YLYGVSWGSRPWEAAVVALSFGVAVSGLSL---MSSALVQNEKTLMAIWPIGIQISSALG 327 Query: 320 GGMAPL---PDSWVNTLGAFTINHWVLQAIIRMML 351 G M PL P + + + T N W LQA ++M+ Sbjct: 328 GSMVPLAAFPPT-MQLVARVTPNFWGLQAFTQVMM 361 >A7Z0X4.1 A7Z0X4_BACA2 YfiM Length = 395 Score = 68.2 bits (165), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 83/358 (23%), Positives = 144/358 (40%), Gaps = 40/358 (11%) Query: 49 TKIEPVRVAVVNPAGDGQSAMIAAFLKLPDIQK---VIVPQDAENRQAAESGLRTGKYGY 105 +K++P + A N D + + LK D+ K +I +D + Q + +++GK Sbjct: 44 SKVKPFQTAFYN--ADHAESSLGDVLK-NDVLKQTDLIRVKDIGSYQEGKQLVKSGKVSV 100 Query: 106 AVIVPDGFDSEVQQGGKAQLEFILGKNHTDN-----MTAGTAFDNFLSTVN--------- 151 V VP GF + K ++ I + + N M A D+F++ V Sbjct: 101 FVYVPKGFAKAAEHNEKTSIKVISDQQGSVNGDIVEMMA----DSFVTRVKAEISEEKAV 156 Query: 152 YKXXXXXXXXXXXXXXXXPSSEDREAVKLGDLNNGGRT--YTASQXXXXXXXXXXXXXXG 209 K S E +AV + + G A Q Sbjct: 157 LKQSGHLSKGDASKIISGLSGEREQAVTIPKQSAGNHAAPIDAMQYYSIAMVVMFSIMTA 216 Query: 210 MTVTNSLFSEKDNHTLHRINSMPVKASELFIGKMLGVGIVSIIQCLAIILLSDWLFGVYW 269 + + + E+ HTL RI SMPV + GK+LG+ + ++Q A+I+ S L+ V W Sbjct: 217 FALIHGIVEERQQHTLFRIKSMPVLRIQYVAGKLLGIMLAILMQMAAVIIASSILYQVKW 276 Query: 270 GNRPGXXXXXXXXMIIASMTMSIVVSMFSKTAAGARSIISL---ITVGMTFISGGMAP-- 324 GN I+ S + +V ++ TA ++ S+ I G +F+ G Sbjct: 277 GN----LFEILLVTIVYSFAIGSIVLLWGFTAKNHETVSSMAAPILYGFSFLGGSFIAKD 332 Query: 325 -LPDSWVNTLGAFTINHWVLQAIIRMMLHSGLPQLTPNLLMISVICLVMLCGAILSYW 381 LPDS + + N + + + + H+GL ++ +L ++ I V LC L+ W Sbjct: 333 GLPDS-LKIVQELIPNGKAINSYVSVTQHAGLSGISTDLAELAAIGAVFLC---LTIW 386 >C1FSH3.1 C1FSH3_CLOBJ Multidrug ABC transporter, permease Length = 372 Score = 63.9 bits (154), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 36/117 (30%), Positives = 58/117 (49%) Query: 209 GMTVTNSLFSEKDNHTLHRINSMPVKASELFIGKMLGVGIVSIIQCLAIILLSDWLFGVY 268 + NS +E +T+ ++ S P E+FIGK+LG+ VS+IQ + + L S + VY Sbjct: 192 SLAAVNSFGNEYTRNTIIKLTSSPASKHEIFIGKVLGLTFVSVIQIILVFLFSKYALKVY 251 Query: 269 WGNRPGXXXXXXXXMIIASMTMSIVVSMFSKTAAGARSIISLITVGMTFISGGMAPL 325 WG G II ++ I + K + A S ++ I +TF+ GG P+ Sbjct: 252 WGKSEGIILLVLLSEIIMAIFAGISIIYLFKKQSVAESFLNGILPFITFLGGGYTPM 308 >B0KBI8.1 B0KBI8_THEP3 ABC-2 type transporter Length = 371 Score = 63.5 bits (153), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 44/153 (28%), Positives = 73/153 (47%), Gaps = 4/153 (2%) Query: 219 EKDNHTLHRINSMPVKASELFIGKMLGVGIVSIIQCLAIILLSDWLFGVYWGNRPGXXXX 278 E+ T RI + P+K E+F+GK+LGV IQ L I+L S ++FG WG Sbjct: 202 ERTKKTGRRILASPIKKYEIFMGKVLGVTFSVFIQALIIVLFSKYIFGANWGKDIVTVFV 261 Query: 279 XXXXMIIASMTMSIVVSMFSKTAAGARSIISLITVGMTFISGGMAPLPD---SWVNTLGA 335 II + + + + K A A+ +++ I + F+ G P+ + ++ L Sbjct: 262 IIISEIIFVVGLGVSLGFIIKNGAMAQGLLNGIIPFIAFLGGSYIPVKEMGSQFIEKLSN 321 Query: 336 FTINHWVLQAIIRMMLHSGLPQLTPNLLMISVI 368 F+ W AI ++L G Q P LM+++I Sbjct: 322 FSPITWTNTAIFNVIL-GGNYQYVPTALMVNLI 353 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,806,937,655 Number of extensions: 62075524 Number of successful extensions: 177891 Number of sequences better than 1.0e-05: 115 Number of HSP's gapped: 178220 Number of HSP's successfully gapped: 115 Length of query: 387 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 249 Effective length of database: 1,760,596,133 Effective search space: 438388437117 Effective search space used: 438388437117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)