BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0118.0005.p (374 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA35.1 B3KA35_9BACI Spore germination protein M 375 e-102 B1DDV2.1 B1DDV2_9BACL Spore germination protein M 258 8e-67 C0ZAF6.1 C0ZAF6_BREBN Germination protein 188 1e-45 Q5WEM2.1 Q5WEM2_BACSK Germination protein GerM 185 8e-45 P39072.2 GERM_BACSU Spore germination protein gerM 182 4e-44 >B3KA35.1 B3KA35_9BACI Spore germination protein M Length = 352 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 193/372 (51%), Positives = 260/372 (69%), Gaps = 29/372 (7%) Query: 2 KHMKPIRGLSAACLLAVPLTLSGCGLF-GSESASVDPPPSEVEAQMLQVSGEGTPDTGVL 60 ++ + +R LSAA LLAVP+ +SGCG+ G +S ++DPPP++VE QML +G Sbjct: 3 RNQRTLRRLSAAGLLAVPIVMSGCGMLSGKQSEAIDPPPAQVEKQMLNTAG--------- 53 Query: 61 GPVALDEADLAAGVSTDTAPAAAAGERTTVFLEDGNGLLAPVSLSLPEGDNTAMLKDSLA 120 TA G +TTV+L D G+LAPV+L +P+ + +++K+SL Sbjct: 54 ----------------STAAQGDIGMQTTVYLADARGMLAPVTLGIPKVEGASVVKESLE 97 Query: 121 ALVSKGQYASALPEGFKGVLPAGTEVKNITVD-KDKLAVVEFNSKFNDYEPADERKILEA 179 LV+ G+YA +P+GF+ VLPAGT+V N+TVD + KLAVVEFN F +Y+ ADER+ILEA Sbjct: 98 VLVNGGRYAGYVPQGFQAVLPAGTQVHNVTVDPESKLAVVEFNKSFAEYKAADERRILEA 157 Query: 180 ITWTLTGQDGIQGVQLWVDGKKLTEMPLQDTPLDRPLTRALGINLPK-HGPLLMNSSAVT 238 +TWTLTG +GI+ +Q+WVDG KLTEMP+ PLDRPL+R++GINL K L NSS VT Sbjct: 158 LTWTLTGHEGIEQMQVWVDGVKLTEMPVNHMPLDRPLSRSMGINLQKADDALYTNSSPVT 217 Query: 239 VYFSAATSDGTHQYYVPVTRFVPAGQDTLKAALNELIAGPESEKGLEMVMTQETVLDSVE 298 VYFS+ T +G QYYVPVTR VP GQD +KAALNELI GP+ GL V+ +T + +VE Sbjct: 218 VYFSSVTPEGI-QYYVPVTRLVPTGQDAVKAALNELIRGPQYGDGLVEVIPDDTTVAAVE 276 Query: 299 AGQNGVVTVSLNDDMFADGKGVPAEMLESVVLTVAQNSDDALVQIRMNGQKTVTGTNNVD 358 G++G VTVSL D MF G+ VP E+L++VVLTVA+N+DDA V+IRMN V GT++ + Sbjct: 277 KGEDGAVTVSLQDSMFEQGEQVPTELLQAVVLTVAENADDAKVKIRMNDLTEVVGTDSRN 336 Query: 359 YGQPVSAPEYVN 370 Y +PVS PE++N Sbjct: 337 YSEPVSRPEFIN 348 >B1DDV2.1 B1DDV2_9BACL Spore germination protein M Length = 354 Score = 258 bits (658), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 160/378 (42%), Positives = 216/378 (57%), Gaps = 32/378 (8%) Query: 1 MKHMKPIRGLSAACLLAVPLTLSGCGLFGSES-ASVDPPPSEVEAQMLQVSGEGTPDTGV 59 M + IR + +L P+ +GCGL ++ +DPP Q + G D Sbjct: 1 MTQTRWIRRAAICGVLVFPILATGCGLISQDAKGEIDPPQ--------QTASTGNVDGTT 52 Query: 60 LGPVALDEADLAAGVSTDTAPAAAAGERTTVFLEDGNGLLAPVSL--SLPEGDNTAMLKD 117 G + P TV+LED NG LAPVSL SL E K Sbjct: 53 TGQAVM--------------PGQDDDSNLTVYLEDRNGYLAPVSLKASLTENAVEEAGKK 98 Query: 118 SLAALVSKGQYASALPEGFKGVLPAGTEVKNITVD-KDKLAVVEFNSKFNDYEPADERKI 176 +L +V G +AS LPE F+ VLP GTEVK++ +D K ++A VEF F DY DER I Sbjct: 99 ALEIMVDGGSFASQLPEDFRAVLPKGTEVKSLHIDQKTQVATVEFGGTFTDYPATDERTI 158 Query: 177 LEAITWTLTGQDGIQGVQLWVDGKKLTEMPLQDTPLDRPLTRALGINL-PKHGPLLMNSS 235 +EAITWTLT G +GV++W DG KL EMP PLD PLTR++GINL G +NS+ Sbjct: 159 VEAITWTLTAM-GAKGVEVWYDGAKLPEMPQGGFPLDEPLTRSVGINLEAADGVNYLNST 217 Query: 236 AVTVYFSAATSDGTHQYYVPVTRFVPAGQDT-LKAALNELIAGPESEKGLEMVMTQETVL 294 VT+YFS+ T++ QYYVPVTR + +T +AA+ +LI GP + K L V+T + + Sbjct: 218 PVTLYFSSQTAND-EQYYVPVTRLINRSDETSAQAAMEQLITGPLNAKNLTAVLTNDVEV 276 Query: 295 DSVEAGQNGVVTVSLNDDMFADGKGVPAEMLESVVLTVAQNSDDALVQIRMNGQKTVTGT 354 +S++ ++ VTV L D F G PAEMLE++VL++ +N+ A VQI +NGQ TVT Sbjct: 277 NSIKQ-KDDTVTVDLKDQAFETGLPAPAEMLEAIVLSITENTGAAQVQITLNGQ-TVTDE 334 Query: 355 NNVDYGQPVSAPEYVNVL 372 NNVDY +PVS P +VN + Sbjct: 335 NNVDYSKPVSRPHHVNAI 352 >C0ZAF6.1 C0ZAF6_BREBN Germination protein Length = 350 Score = 188 bits (477), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 141/376 (37%), Positives = 206/376 (54%), Gaps = 38/376 (10%) Query: 1 MKHMKPIRGLSAACLLAVPLTLSGCGLFG--SESASVDPPP-SEVEAQMLQVSGEGTPDT 57 ++H+ G A L + LSGCGLFG E+ S+D PP SEV + +G +PD Sbjct: 3 IRHI----GKMTAVLGVSAVLLSGCGLFGPEKETISIDAPPQSEVSVDL--PNGTPSPDA 56 Query: 58 GVLGPVALDEADLAAGVSTDTAPAAAAGERTTVFLEDGNGLLAPVSLSLPEGDNTAMLKD 117 D A V +T GER V+L+D NG + PVS++LP+ D A K Sbjct: 57 A---------KDGAQPVVQET------GERM-VYLQDANGYIVPVSMTLPKSDGPA--KQ 98 Query: 118 SLAALVSKGQYASALPEGFKGVLPAGTEVKNITVDKDKLAVVEFNSKFNDYEPADERKIL 177 L+ +V G S L GF VLP GTEVK + + K+ +A V+F+ F YE DE+KI+ Sbjct: 99 VLSYMVKGGPVESMLQGGFSAVLPKGTEVKGLVI-KNGVATVDFSKDFKTYEEKDEKKIV 157 Query: 178 EAITWTLTGQDGIQGVQLWVDGKKLTEMPLQDTPLDRPLTRALGINLP-KHGPLLMNSSA 236 +A+T LT ++ VQ+WV+G LTEMP TP+ L R G+NL G + ++S+ Sbjct: 158 DAVTRALTEFSTVKTVQIWVNGTLLTEMPATKTPITL-LDRNQGVNLELGDGAVPGDTSS 216 Query: 237 VTVYFSAATSDGTHQYYVPVTRFVPAGQDTLKAALNELIAGPESEKGLEMVMTQETVLDS 296 VTVYF D T YYVPVTR VP D KA + ELI GP+ L + + T + + Sbjct: 217 VTVYFQGQLDD-TRTYYVPVTRLVPETTDIAKATVEELIKGPKQGSQLFSSLLRTTRVLN 275 Query: 297 VEAGQNGVVTVSLNDDM--FADGKGVPAEMLESVVLTVAQNSDDALVQIRMNGQKTVTGT 354 V+ ++ +VTV+L+ D+ + DGK E L+S+VL++ + + VQ+ + G+ +G Sbjct: 276 VKQEKD-LVTVNLSSDILKYDDGKEASPEALQSLVLSLTETTGAKQVQLLVEGKPLASGA 334 Query: 355 NNVDYGQPVSAPEYVN 370 + +PVS P +N Sbjct: 335 ----FTKPVSRPVQLN 346 >Q5WEM2.1 Q5WEM2_BACSK Germination protein GerM Length = 377 Score = 185 bits (469), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 135/384 (35%), Positives = 213/384 (55%), Gaps = 20/384 (5%) Query: 4 MKPIRGLSAACLLAVPLTLSGCGLFGSESASV--DPPPSEVEAQMLQVSGEGTPDTGVLG 61 M+ + G+SA ++ + LT +GC L +++ +PP + +A+ + E P+ V Sbjct: 1 MRKLAGVSAPFVVVLALTTTGCSLGANDAVDTLDEPPVTYTDAEEDFGNEENEPEEDVTE 60 Query: 62 PVA-----LDEADLAAGVSTDTAP--AAAAGERTTVFLEDGNGLLAPVSLSLPEGDNTAM 114 A +E G + +T P AA + + ++L D NGL+ P +L+LP+ D ++ Sbjct: 61 EQADESEAGEETGEETGSTDETTPDEAAESLNQRELYLIDSNGLVVPQTLTLPKTD--SV 118 Query: 115 LKDSLAALVSKGQYASALPEGFKGVLPAGTEVKNITVDKDKLAVVEFNSKFNDYEPADER 174 +K +L LV G LP GF+ VLPAGTEV + ++KLA+V F+S+FNDY ADE+ Sbjct: 119 MKQALEYLVEGGPINDILPNGFRAVLPAGTEVDIDHLKEEKLAIVNFSSEFNDYNLADEK 178 Query: 175 KILEAITWTLTGQDGIQGVQLWVDGKKLTEMPLQDTPLDRPLTRALGINLPKHGPL-LMN 233 +I EA+TWTLT ++ V++ V+G +L +MP + PL L+RA GIN+ + N Sbjct: 179 QIFEAVTWTLTQFPDVEEVKVEVNGYELDKMPQSEKPLVGNLSRADGINVDTSEVVDFTN 238 Query: 234 SSAVTVYFSAATSDGTHQYYVPVTRFVPAGQDTLKAALNELIAGPESEKGLEMVMTQETV 293 S+ +T+YF A + T YYVPVT+ V + L+AA+NELI GP L M+ + Sbjct: 239 STDITLYFLGANDEDT--YYVPVTKRVENVDNELEAAINELIDGPSLMTNLLTEMSGDVE 296 Query: 294 LDSVEAGQNGVVTVSLNDDMFA--DGKGVPAEMLESVVLTVAQNSDDALVQIRMNGQKTV 351 L + QNG V + N+ + + +G +P +LES+ LT+ + V I++NG+ Sbjct: 297 LLNEPKLQNGEVVLDFNEAIQSANEGSAIPTSVLESLALTLTEQGGIEKVSIQVNGE--- 353 Query: 352 TGTNNVDYG-QPVSAPEYVNVLPL 374 G N + V+ PE VN PL Sbjct: 354 AGVNEAGIEVEEVTRPELVNAKPL 377 >P39072.2 GERM_BACSU Spore germination protein gerM Length = 366 Score = 182 bits (462), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 125/369 (33%), Positives = 197/369 (53%), Gaps = 23/369 (6%) Query: 10 LSAACLLAVPLTLSGCGLFGSESAS--VDPPPSEVEAQMLQVSGEGTPDTGVLGPVALDE 67 + A CL + L LSGCGLF S+ A+ +DPP + + +T ++ Sbjct: 9 IGATCLTSA-LLLSGCGLFQSDKAAEEIDPPQ---DVTFVNDEAGANSNTTAAKKTESEK 64 Query: 68 ADLAAGVSTDTAPAAAAGERTTVFLEDGNGLLAPVSLSLPEGDNTAMLKDSLAALVSKGQ 127 +D A D A + E ++L D NG + +L LP+ ++TA K +L LV G Sbjct: 65 SDTAKA---DQASSTVMRE---LYLIDKNGYVVAQTLPLPKSESTA--KQALEYLVQGGP 116 Query: 128 YASALPEGFKGVLPAGTEVKNITVDKDKLAVVEFNSKFNDYEPADERKILEAITWTLTGQ 187 + LP GF+ VLPA T V N+ + KD A+ +F+++F +Y+ DE+KI++++TWTLT Sbjct: 117 VSEILPNGFRAVLPADTTV-NVDIKKDGTAIADFSNEFKNYKKEDEQKIVQSVTWTLTQF 175 Query: 188 DGIQGVQLWVDGKKLTEMPLQDTPLDRPLTRALGINLPKHG-PLLMNSSAVTVYFSAATS 246 I V+L ++G +L EMP+ TP+ L+R GINL G L + +TVY+ A Sbjct: 176 SSIDKVKLRINGHELKEMPVGGTPISDDLSRKDGINLETAGVNDLTATHPLTVYYLAENE 235 Query: 247 DGTHQYYVPVTRFVP-AGQDTLKAALNELIAGPESEKGLEMVMTQETVLDSVEAGQNGVV 305 D +YYVPVT+ + + +D + AA+NEL GP GL +++ L S ++G V Sbjct: 236 DS--EYYVPVTKRIDNSEKDDITAAINELAKGPSKVSGLLTDFSEDVKLVSKPKIKDGRV 293 Query: 306 TVSLNDDMFADG----KGVPAEMLESVVLTVAQNSDDALVQIRMNGQKTVTGTNNVDYGQ 361 T+ N +F K + +E+L S+VLT+ + D V +++NG+ + + Sbjct: 294 TLDFNQSIFGSADEKTKMISSEVLNSIVLTLTEQPDVKSVSVKVNGKSELVNEKGEKLTE 353 Query: 362 PVSAPEYVN 370 PVS P VN Sbjct: 354 PVSRPSQVN 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.131 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,319,281,223 Number of extensions: 101621663 Number of successful extensions: 226333 Number of sequences better than 1.0e-05: 103 Number of HSP's gapped: 225917 Number of HSP's successfully gapped: 103 Length of query: 374 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 237 Effective length of database: 1,770,017,148 Effective search space: 419494064076 Effective search space used: 419494064076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)