BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0117.0008.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEI6.1 B3KEI6_9BACI Aminoglycoside N(3')-acetyltransferase EC=... 284 5e-75 C2S4S4.1 C2S4S4_BACCE Putative uncharacterized protein 274 5e-72 B7HUN6.1 B7HUN6_BACC7 Aminoglycoside N3-acetyltransferase EC=2.3... 273 1e-71 Q9RWR7.1 Q9RWR7_DEIRA Aminoglycoside N3-acetyltransferase 272 2e-71 B7JST1.1 B7JST1_BACC0 Aminoglycoside N3-acetyltransferase EC=2.3... 272 3e-71 >B3KEI6.1 B3KEI6_9BACI Aminoglycoside N(3')-acetyltransferase EC=2.3.1.81 Length = 266 Score = 284 bits (727), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 142/260 (54%), Positives = 181/260 (69%), Gaps = 2/260 (0%) Query: 9 ITVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQ 68 IT++ ++ D LGV++GMTLL+HSS KS+G W+ GGP AVILALE VLG+EGTLVMPTQ Sbjct: 7 ITIKDISDDLAALGVRKGMTLLVHSSLKSVGGWIVGGPEAVILALEDVLGEEGTLVMPTQ 66 Query: 69 SSDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPI 128 S++LTDP W NPP WW IRE MP YDPDLT+ GMG+IPE FRKQ+GV RS+HP Sbjct: 67 SANLTDPKTWGNPPADPKWWNLIREGMPPYDPDLTVTSGMGIIPEVFRKQEGVIRSAHPH 126 Query: 129 SSFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAE 188 SFAA GR ++ H L+++ GE SPLAR+Y+ VL+LG +NTS HL+EYRA Sbjct: 127 VSFAARGREARALMQSHPLDHSLGEASPLARLYEAGAYVLILGSGYGNNTSFHLSEYRAN 186 Query: 189 YAGKQEVTAGAPM-LVDGARQWVEFRDYNWNSDDFAAIGRDF-GQETGQITYGKVAAAEA 246 + GK+ VTA AP+ V G W F+D N++SDDF A+G F Q +G+V A+ Sbjct: 187 WKGKEAVTAHAPVRRVQGVTVWEAFQDINYHSDDFEALGASFEAQCPDAYIHGRVGKADC 246 Query: 247 QLIPQREIVDYAVKWLEQRR 266 L QR +VDYAV W+E+ R Sbjct: 247 ILAEQRAMVDYAVSWMEKNR 266 >C2S4S4.1 C2S4S4_BACCE Putative uncharacterized protein Length = 292 Score = 274 bits (701), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 128/257 (49%), Positives = 183/257 (71%), Gaps = 4/257 (1%) Query: 10 TVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQS 69 T++++ D RKLG+++GMT+++HSS S+G WV+GG VAV+ AL V+ +EGTL+MPTQS Sbjct: 40 TIKTITNDLRKLGLEKGMTVIVHSSLSSIG-WVSGGAVAVVEALMNVITEEGTLIMPTQS 98 Query: 70 SDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPIS 129 SDL+DP WS PPVPE WWQ IR+++PAYDP +T RGMG I E FR V RS+HP+ Sbjct: 99 SDLSDPKHWSRPPVPEDWWQIIRDNVPAYDPRITPTRGMGEIVECFRTYPNVVRSNHPLG 158 Query: 130 SFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAEY 189 SFAAWGRH +EI +L + GE+SPL +IYDL G +LL+G+ SNTS+HL+E R+ Sbjct: 159 SFAAWGRHAEEITMNQSLSMSLGEESPLRKIYDLDGYILLIGVGYDSNTSVHLSEVRS-- 216 Query: 190 AGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQITYGKVAAAEAQLI 249 + + GAP++ +G R W EF D +++SD F IG +F Q+ G +T G++ A+ +L+ Sbjct: 217 GACELIKVGAPIIENGERVWKEFVDMDYDSDKFVEIGVEFEQK-GTVTIGEIGNAKCRLM 275 Query: 250 PQREIVDYAVKWLEQRR 266 QR+IVD+ +W ++ Sbjct: 276 KQRDIVDFGTEWFRKKN 292 >B7HUN6.1 B7HUN6_BACC7 Aminoglycoside N3-acetyltransferase EC=2.3.1.81 Length = 267 Score = 273 bits (698), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 128/257 (49%), Positives = 183/257 (71%), Gaps = 4/257 (1%) Query: 10 TVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQS 69 T++++ D RKLG+++GMT+++HSS S+G WV+GG VAV+ AL V+ +EGTL+MPTQS Sbjct: 15 TIKTITNDLRKLGLEKGMTVIVHSSLSSIG-WVSGGAVAVVEALMNVITEEGTLIMPTQS 73 Query: 70 SDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPIS 129 SDL+DP WS PPVPE WWQ IR+++PAYDP +T RGMG I E FR V RS+HP+ Sbjct: 74 SDLSDPKHWSRPPVPEDWWQIIRDNVPAYDPRITPTRGMGEIVECFRTYPNVVRSNHPLG 133 Query: 130 SFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAEY 189 SFAAWGRH +EI +L + GE+SPL +IYDL G +LL+G+ SNTS+HL+E R+ Sbjct: 134 SFAAWGRHAEEITMNQSLSMSLGEESPLRKIYDLDGYILLIGVGYDSNTSVHLSEVRS-- 191 Query: 190 AGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQITYGKVAAAEAQLI 249 + + GAP++ +G R W EF D +++SD F IG +F Q+ G +T G++ A+ +L+ Sbjct: 192 GACELIKVGAPIIENGERVWKEFVDMDYDSDKFVEIGVEFEQK-GTVTIGEIGNAKCRLM 250 Query: 250 PQREIVDYAVKWLEQRR 266 QR+IVD+ +W ++ Sbjct: 251 KQRDIVDFGTEWFRKKN 267 >Q9RWR7.1 Q9RWR7_DEIRA Aminoglycoside N3-acetyltransferase Length = 279 Score = 272 bits (696), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 143/251 (56%), Positives = 177/251 (70%), Gaps = 6/251 (2%) Query: 10 TVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQS 69 T SLAAD R LGVQ G LL+HSS LG WVAGG VAVI AL+ VL +GTLVMPT S Sbjct: 21 TRASLAADLRSLGVQAGDVLLVHSSLSRLG-WVAGGAVAVIQALQDVLTPQGTLVMPTHS 79 Query: 70 SDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPIS 129 SDLTDP+GW +PPVPEAWW+ IR +PA+DP LT RGMG IPE FR GV+RS HP S Sbjct: 80 SDLTDPAGWLSPPVPEAWWEVIRAELPAFDPALTPTRGMGRIPELFRTWPGVRRSDHPHS 139 Query: 130 SFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAEY 189 SFAAWGRH + + GHAL ++ GE SPLARIYDL G VLLLG + NT+LHLAE RA Sbjct: 140 SFAAWGRHAEFVTAGHALAFSLGEGSPLARIYDLNGRVLLLGTE--RNTNLHLAEVRAGV 197 Query: 190 AGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQITYGKVAAAEAQLI 249 + V P++VDG RQW+ F + +++++ FA + F + G + G V +A+A+L+ Sbjct: 198 --RPTVAVSGPVMVDGERQWLTFDEPDYDAEAFAPVKAAF-EAAGGVVRGTVGSAQARLM 254 Query: 250 PQREIVDYAVK 260 QRE+VD+AV Sbjct: 255 SQRELVDFAVN 265 >B7JST1.1 B7JST1_BACC0 Aminoglycoside N3-acetyltransferase EC=2.3.1.81 Length = 267 Score = 272 bits (695), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 123/257 (47%), Positives = 185/257 (71%), Gaps = 4/257 (1%) Query: 10 TVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQS 69 T++++ D RKLG+++GMT+++HSS S+G W++GG VAV+ AL +V+ +EGT++MPTQS Sbjct: 15 TIKTITNDLRKLGLKKGMTVIVHSSLSSIG-WISGGAVAVVEALMEVITEEGTIIMPTQS 73 Query: 70 SDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPIS 129 SDL+DP WS PPVPE WWQ IR+++PA++P +T R MG + E FR V RS+HP+ Sbjct: 74 SDLSDPKHWSRPPVPEEWWQIIRDNVPAFEPHITPTRAMGKVVECFRTYPNVLRSNHPLG 133 Query: 130 SFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAEY 189 SFAAWGRH +EI + +L + GE+SPL +IYDL G +LL+G+ SNTS+HL+E R+ Sbjct: 134 SFAAWGRHAEEITENQSLSMSLGEESPLRKIYDLDGYILLIGVGYDSNTSVHLSEVRS-- 191 Query: 190 AGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQITYGKVAAAEAQLI 249 + + GAP++ +G R W EF D +++SD F IG +F Q+ G +T GK+ A+ +L+ Sbjct: 192 GACELIKVGAPIIENGERVWKEFVDMDYDSDKFVEIGVEFEQK-GTVTMGKIGNAKCRLM 250 Query: 250 PQREIVDYAVKWLEQRR 266 QR+IVD+ ++W ++ Sbjct: 251 KQRDIVDFGIEWFRKKN 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,752,723,258 Number of extensions: 76644255 Number of successful extensions: 164926 Number of sequences better than 1.0e-05: 217 Number of HSP's gapped: 164418 Number of HSP's successfully gapped: 217 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)