BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0001.p (425 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKE2.1 B3KKE2_9BACI Radical SAM domain protein 718 0.0 B1DK73.1 B1DK73_9BACL Radical SAM domain protein 667 0.0 C0Z833.1 C0Z833_BREBN Putative uncharacterized protein 596 e-168 Q0AUB0.1 Q0AUB0_SYNWW Radical SAM domain protein 355 4e-96 B7AZ52.1 B7AZ52_9FIRM Putative uncharacterized protein 344 1e-92 >B3KKE2.1 B3KKE2_9BACI Radical SAM domain protein Length = 425 Score = 718 bits (1853), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/421 (81%), Positives = 374/421 (88%) Query: 1 MYLVYADEQGNVYDHSELYGLARSGDXXXXXXXXXXXXXXXGATLVGLPNTRAVGMNPKT 60 MYLVYADEQGNVYDHSEL+GLARSGD GATLVGLP+TRA+GM+P+T Sbjct: 1 MYLVYADEQGNVYDHSELFGLARSGDMIVEILEDELIPLPEGATLVGLPSTRAIGMDPET 60 Query: 61 GEMLPLPEGSQAVGALLPQGFTRLCLPGYVKTDKSYKLPLFGYSAVVWKDGGFYVAADLT 120 G+M+PL +G AVGALLPQG+TRLCLPGYVK+DK YKLPLFGYSAVVWKDG FY A L+ Sbjct: 61 GDMMPLEDGYTAVGALLPQGYTRLCLPGYVKSDKEYKLPLFGYSAVVWKDGAFYTTARLS 120 Query: 121 DDPEQWNPLNCDREDVEAGVGSLTAKYPGNRLYGHLSNCALGYECLTSSNTFLGRWEGAV 180 DDPE+WNP+NCD +D+E GV +L KYP NRLY HLSNCALGYECLT+SNTFL RWEGAV Sbjct: 121 DDPEKWNPVNCDLDDLEHGVKTLKEKYPENRLYEHLSNCALGYECLTASNTFLNRWEGAV 180 Query: 181 PVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRPTVNEISEVMLEHLKTPQSIISFGQGC 240 PVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRP V+E+ +VMLEHLKTP+SIISFGQGC Sbjct: 181 PVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRPRVDELVQVMLEHLKTPESIISFGQGC 240 Query: 241 EGEPSTQAKLIIEAIREVRSITDMGYININTNAGLSDHIRGIVDAGLDLMRVSTISALDD 300 EGEPSTQAK+IIEA+REVR+ TDMGYININTNAGL+D IRGIVDAGLDLMRVSTISALD Sbjct: 241 EGEPSTQAKIIIEAMREVRAQTDMGYININTNAGLTDFIRGIVDAGLDLMRVSTISALDA 300 Query: 301 HYNAYYKPRGYTLANVEKSLKYAASQGVYTSINYLIFPGVTDREEEIEAMVEFVRRTDLK 360 HYNAYYKPRGYTLANVEKSL+YA QGVYTSINYLIFPGVTDREEE+EAM+EF RRT LK Sbjct: 301 HYNAYYKPRGYTLANVEKSLRYATDQGVYTSINYLIFPGVTDREEELEAMIEFARRTGLK 360 Query: 361 LIQMRNLNIDPESYLELIPPAQGEILGMKTMLEIFREELPDVVIGSYTHVPPADLARAKQ 420 LIQ+RNLNIDPESYL LIPPAQGEILGMK +EI +EELPDVVIGSYTHVPPA AR K+ Sbjct: 361 LIQLRNLNIDPESYLGLIPPAQGEILGMKQAIEIMQEELPDVVIGSYTHVPPAGQARPKK 420 Query: 421 R 421 R Sbjct: 421 R 421 >B1DK73.1 B1DK73_9BACL Radical SAM domain protein Length = 417 Score = 667 bits (1722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/413 (77%), Positives = 354/413 (85%) Query: 1 MYLVYADEQGNVYDHSELYGLARSGDXXXXXXXXXXXXXXXGATLVGLPNTRAVGMNPKT 60 M LVYADE+GNV+DH + L RSGD GATLVGLP+TR V M+P T Sbjct: 1 MNLVYADEKGNVFDHPDYIALGRSGDMIVELMEEELIPLPRGATLVGLPHTRPVFMDPDT 60 Query: 61 GEMLPLPEGSQAVGALLPQGFTRLCLPGYVKTDKSYKLPLFGYSAVVWKDGGFYVAADLT 120 G+M L QAVGALLPQG+TRL LP YVKTDK+ K PLFGY++VVWKDGGFYVAA+ + Sbjct: 61 GDMKMLSGSYQAVGALLPQGYTRLALPSYVKTDKNEKFPLFGYTSVVWKDGGFYVAAEQS 120 Query: 121 DDPEQWNPLNCDREDVEAGVGSLTAKYPGNRLYGHLSNCALGYECLTSSNTFLGRWEGAV 180 DDP +W+PLNCDR DV GV LTAKYP NRLY HLS+CALGYECLTSSNTFL RWEG V Sbjct: 121 DDPYKWDPLNCDRGDVRKGVEQLTAKYPENRLYKHLSHCALGYECLTSSNTFLQRWEGGV 180 Query: 181 PVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRPTVNEISEVMLEHLKTPQSIISFGQGC 240 PVSYSCNAGCFGCISEQPDDSGFV+PQTRMNF+PTV+EI EVMLEHLKTP+SIISFGQGC Sbjct: 181 PVSYSCNAGCFGCISEQPDDSGFVAPQTRMNFKPTVDEIVEVMLEHLKTPESIISFGQGC 240 Query: 241 EGEPSTQAKLIIEAIREVRSITDMGYININTNAGLSDHIRGIVDAGLDLMRVSTISALDD 300 EGEPSTQAK+I+EA++ VRS T+ GYININTNAGL+D +R IVDAGLDLMRVSTISA+D+ Sbjct: 241 EGEPSTQAKIIVEAMKIVRSQTNQGYININTNAGLTDFMRAIVDAGLDLMRVSTISAIDE 300 Query: 301 HYNAYYKPRGYTLANVEKSLKYAASQGVYTSINYLIFPGVTDREEEIEAMVEFVRRTDLK 360 HYNAYYKPR YTLANVEKSLKYA +GVYTSINYLIFPGVTDREEEIEAM+ F +RT LK Sbjct: 301 HYNAYYKPRAYTLANVEKSLKYATDKGVYTSINYLIFPGVTDREEEIEAMIGFAKRTGLK 360 Query: 361 LIQMRNLNIDPESYLELIPPAQGEILGMKTMLEIFREELPDVVIGSYTHVPPA 413 LIQMRNLNIDPESYL+LIPPA+GEI GMK MLEIFREELPDVVIGSYTH+PPA Sbjct: 361 LIQMRNLNIDPESYLQLIPPAKGEIYGMKQMLEIFREELPDVVIGSYTHLPPA 413 >C0Z833.1 C0Z833_BREBN Putative uncharacterized protein Length = 432 Score = 596 bits (1537), Expect = e-168, Method: Compositional matrix adjust. Identities = 279/419 (66%), Positives = 335/419 (79%), Gaps = 1/419 (0%) Query: 1 MYLVYADEQGNVYDHSELYGLARSGDXXXXXXXXXXXXXXXGATLVGLPNTRAVGMNPKT 60 MYLVYADE+GNVYDH L+ +AR+GD G+TLV LP+T +GM+P T Sbjct: 12 MYLVYADEKGNVYDHPGLFAVARNGDILTEILEEELIPLPEGSTLVSLPDTEPIGMDPDT 71 Query: 61 GEMLPLPEGSQAVGALLPQGFTRLCLPGYVKTDKSYKLPLFGYSAVVWKDGGFYVAADLT 120 GEM+ L +G AVGAL+PQG TRL LPGYVKT+K KLPLFGYSAVVWKD F+V + Sbjct: 72 GEMVKL-DGCTAVGALVPQGITRLLLPGYVKTNKESKLPLFGYSAVVWKDNRFWVTGRAS 130 Query: 121 DDPEQWNPLNCDREDVEAGVGSLTAKYPGNRLYGHLSNCALGYECLTSSNTFLGRWEGAV 180 DD +W+PLN +++ V +P NR+ HLS+CAL YECLT+SN F RWEG++ Sbjct: 131 DDIYKWDPLNFPMDELRQRVAKTLETFPQNRILNHLSHCALEYECLTASNNFFNRWEGSL 190 Query: 181 PVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRPTVNEISEVMLEHLKTPQSIISFGQGC 240 PVSY+CNAGC+GCISEQPDDSGF SPQTRMNF+PT +E+ EVML HLKTP+SIISFGQGC Sbjct: 191 PVSYTCNAGCYGCISEQPDDSGFPSPQTRMNFKPTEDELVEVMLHHLKTPESIISFGQGC 250 Query: 241 EGEPSTQAKLIIEAIREVRSITDMGYININTNAGLSDHIRGIVDAGLDLMRVSTISALDD 300 EGEPST A +I+ A+R VR TDMG+ININTNAGL+DHI+GIVDAGLDLMRVS ISA+D+ Sbjct: 251 EGEPSTMASIIVPAMRRVRETTDMGFININTNAGLTDHIKGIVDAGLDLMRVSIISAIDE 310 Query: 301 HYNAYYKPRGYTLANVEKSLKYAASQGVYTSINYLIFPGVTDREEEIEAMVEFVRRTDLK 360 HYNAYY+PR YTL NV +S +YAAS+GVYTSINYL FPGV DREEE+EAM+EF+RRT +K Sbjct: 311 HYNAYYRPRNYTLENVARSAEYAASKGVYTSINYLCFPGVFDREEEMEAMIEFIRRTGIK 370 Query: 361 LIQMRNLNIDPESYLELIPPAQGEILGMKTMLEIFREELPDVVIGSYTHVPPADLARAK 419 LIQ+RNLNIDPESYL +IP AQGE+ GMK +EI+++ELPDVVIGS+TH+PP L R K Sbjct: 371 LIQLRNLNIDPESYLAMIPKAQGEVFGMKQAIEIYQQELPDVVIGSFTHIPPDQLQRRK 429 >Q0AUB0.1 Q0AUB0_SYNWW Radical SAM domain protein Length = 418 Score = 355 bits (912), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 189/417 (45%), Positives = 256/417 (61%), Gaps = 11/417 (2%) Query: 1 MYL-VYADEQGNVYDHSELYGLARSGDXXXXXXXXXXXXXXXGATLVGLPNTRAVGMNPK 59 MYL +YA+E+G V DH + L RSG+ G++LV +P VG++ K Sbjct: 1 MYLSLYANEKGEVLDHPNILMLGRSGNEWLLPEAGEMMPLPRGSSLVRIPGNIPVGLDMK 60 Query: 60 TG----EMLPLPEGSQA--VGALLPQGFTRLCLPGYVKTDKSYKLPLFGYSAVVWKDGGF 113 E PL G +A V ALLPQGFTR LP V + LPL GY+AV K+G Sbjct: 61 ERLAYFEYDPLVSGQRAYAVAALLPQGFTRTLLPACVGSSGEALLPLLGYAAVGLKEGKI 120 Query: 114 YVAADLTDDPEQWNPLNCDREDVEAGVGSLTAKYPGNRLYGHLSNCALGYECLTSSNTFL 173 YVAA +D+ +W+P + E + A + + KYPGN + L+ C+L Y C T+ N F Sbjct: 121 YVAAVQSDEHRKWHPHYYNTERLPAKINRMLKKYPGNMILHQLARCSLEYGCFTAQNIFY 180 Query: 174 GRWEGAVPVSYSCNAGCFGCISEQPDDSGFVSPQTRMNFRPTVNEISEVMLEHL-KTPQS 232 RWEG +P +CNA C CISE D G SPQ R++F PTV EI EV EHL + + Sbjct: 181 QRWEGGIPSMKACNAACIACISE--DHCGTASPQNRLDFAPTVQEIVEVASEHLLRARDA 238 Query: 233 IISFGQGCEGEPSTQAKLIIEAIREVRSITDMGYININTNAGLSDHIRGIVDAGLDLMRV 292 IISFGQGCEGEP+ K + +AIR +R + G IN+NTNAG + ++ + DAG+D MRV Sbjct: 239 IISFGQGCEGEPALNGKRLSQAIRRIRDLCAQGTINMNTNAGYCEGVKLMTDAGMDSMRV 298 Query: 293 STISALDDHYNAYYKPRGYTLANVEKSLKYAASQGVYTSINYLIFPGVTDREEEIEAMVE 352 + SA++++Y Y+ P+ Y+LA+VE S+ YA GV S+N L FPG TDR EE+EA++E Sbjct: 299 TIFSAIEENYMTYHCPQDYSLADVEYSITYAREHGVKISLNLLTFPGFTDRMEEVEALLE 358 Query: 353 FVRRTDLKLIQMRNLNIDPESYLELIPPAQGEILGMKTMLEIFREELPDVVIGSYTH 409 FVR+ + +IQ RNLNIDPE +L I P + +LG+ +++ ELP V IGSYTH Sbjct: 359 FVRKNQVDMIQFRNLNIDPE-FLMRIFPGKSPVLGISNFVKLLESELPGVRIGSYTH 414 >B7AZ52.1 B7AZ52_9FIRM Putative uncharacterized protein Length = 416 Score = 344 bits (882), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 179/407 (43%), Positives = 252/407 (61%), Gaps = 6/407 (1%) Query: 4 VYADEQGNVYDHSELYGLARSGDXXXXXXXXXXXXXXXGATLVGLPNTRAVGMNPKTGEM 63 VYADE GN+ D + G+ R+G A L+ LP+ AVG + GE Sbjct: 11 VYADEDGNILDAPGMRGMGRTGRENILLKPEDLIPLPESADLMLLPDRLAVGQS-ADGET 69 Query: 64 LPLPEGSQAVGALLPQGFTRLCLPGYVKTDKSYKLPLFGYSAVVWKDGGFYVAADLTDDP 123 +P+ AV A+LP G+TRL +P Y + +++ +LPL+GY+AVV AA TD+ Sbjct: 70 MPI--SGLAVAAILPAGYTRLYMPAYERAEEAQRLPLYGYTAVVVYKDELCCAAVYTDEN 127 Query: 124 EQWNPLNCDREDVEAGVGSLTAKYPGNRLYGHLSNCALGYECLTSSNTFLGRWEGAVPVS 183 E+W+P++ + +++ V PGNR+ + C+L + C T+ N F RWE +P S Sbjct: 128 EKWDPVHYNTKELAKLVKRTKKDLPGNRIVEQVGGCSLKWHCCTAQNLFYRRWECGIPTS 187 Query: 184 YSCNAGCFGCISEQPDDSGFVSPQTRMNFRPTVNEISEVMLEHLKT-PQSIISFGQGCEG 242 CNA CFGCIS QP + SPQ R+ FRPT EI+EV + HL P I+SFGQGCEG Sbjct: 188 PVCNANCFGCISLQPAEC-CPSPQERIKFRPTPQEIAEVGIYHLSVAPDGIVSFGQGCEG 246 Query: 243 EPSTQAKLIIEAIREVRSITDMGYININTNAGLSDHIRGIVDAGLDLMRVSTISALDDHY 302 EPS A I I+ +R+ T G IN+N+NAG ++ I+ IVDAGLD +RVS ISA D+ Y Sbjct: 247 EPSLAADNIAAGIQLIRAKTKKGQINMNSNAGWTEGIKKIVDAGLDSLRVSIISARDEAY 306 Query: 303 NAYYKPRGYTLANVEKSLKYAASQGVYTSINYLIFPGVTDREEEIEAMVEFVRRTDLKLI 362 +AYY+ Y L +V+ S++YA GVY S+N L FPG DREEE+ A F R +++I Sbjct: 307 DAYYRA-SYHLDDVKASIRYALDHGVYVSLNLLYFPGFNDREEELAAWQAFFRDLPVQMI 365 Query: 363 QMRNLNIDPESYLELIPPAQGEILGMKTMLEIFREELPDVVIGSYTH 409 Q+RNLNIDP+++L+++P QG+ILG + + EE P +VIGS++H Sbjct: 366 QVRNLNIDPDAFLDIMPEPQGKILGTRHFIAALHEEFPQLVIGSFSH 412 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,578,349,693 Number of extensions: 111363252 Number of successful extensions: 308567 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 308363 Number of HSP's successfully gapped: 47 Length of query: 425 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 287 Effective length of database: 1,760,596,133 Effective search space: 505291090171 Effective search space used: 505291090171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)