BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0112.0029.p (629 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG50.1 B3KG50_9BACI Peptidase S8 and S53 subtilisin kexin sedo... 752 0.0 B1DFM8.1 B1DFM8_9BACL Peptidase S8 and S53 subtilisin kexin sedo... 641 0.0 C5D2M0.1 C5D2M0_9BACI Peptidase S8 and S53 subtilisin kexin sedo... 411 e-113 Q2BAU2.1 Q2BAU2_9BACI Subtilisin-type proteinase 407 e-111 Q5KZT4.1 Q5KZT4_GEOKA Subtilisin-type proteinase EC=3.4.21.- 390 e-106 >B3KG50.1 B3KG50_9BACI Peptidase S8 and S53 subtilisin kexin sedolisin Length = 640 Score = 752 bits (1941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/626 (57%), Positives = 456/626 (72%), Gaps = 12/626 (1%) Query: 9 AGLITAAFTIAVFTYTLRPDGGPALKQASVPNPHQEKTIKKKALTQDVSSTDRLNRTDVS 68 A +I A + + +P P + + P QE K + D+ +TD+LNR D Sbjct: 13 ASVIGALLILLFIPGSKQP--APTAEPSRPLKPQQENKAKHFTMLGDIQATDQLNRLDAR 70 Query: 69 KHLRTMLSETDGKTPEDISAYAKQLQQGHGHIAMLMWIDFRTKQSSTFKSSFPEGSDQEN 128 HLR ML+ ++ + AY ++ Q+ H H + W + + + + + P+ S + Sbjct: 71 GHLRKMLNNDSLRSAGQVKAYTQKEQKMHKHFQQVRWYNLKENKMADIQGQLPKMSKTDK 130 Query: 129 KRLLKYLNTAKTAIQRHQSYESPSFIIGNKKYYFTAQRDREGHYGVIALINQRILDRVAE 188 R+ + A+ ++R + Y+SP F + Y+ A+ ++G YG AL+NQ +L V+ Sbjct: 131 NRIAHHFQLAQQKLKRGEPYDSPKFNAAGQPYFVVAEPSKDGRYGAAALVNQAVLQEVSN 190 Query: 189 HQLKNLRLIPYPKEGKYRVESVHADTLKELTVKTGHDNENASHYYENEIVVRFKNGHPTA 248 HQ +NLRLIPYPKEGK+RVES+HADTL ++TVKTGHDNE ASH+YENEIVV F+N P+ Sbjct: 191 HQKRNLRLIPYPKEGKFRVESIHADTLSDITVKTGHDNEKASHFYENEIVVSFQN-EPSK 249 Query: 249 GQLQTIAADIRCKEPRKLGYAYIFRSGKLNYPQLKAYFDQKWNPQYTEPHYMYLTNDT-- 306 QL I+ +I C+ PRKLGYAY+FRS ++Y +L++YF QKW +YTEPHY+YLTND Sbjct: 250 QQLLDISKEISCETPRKLGYAYVFRSKDMDYQKLRSYFQQKWKIKYTEPHYIYLTNDRSP 309 Query: 307 -------VGENTGAAVITPNDLLFSTYQWNLPAIETEQGWNLSKGSKEVIVAVVDTGVQA 359 + T A V PNDLLFS YQWNLP IET +GW+LSKGS++VI+AV+DTGV Sbjct: 310 QSRVNSDFPDGTNAQVNIPNDLLFSEYQWNLPVIETNRGWDLSKGSEDVIIAVIDTGVDL 369 Query: 360 NHPDLKGQLLAGYNAITNTSPPNDDVGHGTHVAGIIGALTNNGEGVAGISWYNKILPVKA 419 H DL GQL+ GYN + P DDVGHGTHVAGIIGAL NNGEGVAG+SWYNKI+PVKA Sbjct: 370 QHSDLDGQLVEGYNIVQKDKQPLDDVGHGTHVAGIIGALVNNGEGVAGVSWYNKIMPVKA 429 Query: 420 LDSSGAGTTYSVAEGIIWATDKGAKVINLSLGNYADSQFLHDAVKYAYDRDVVLVSAAGN 479 LD SG+GTTY+VAEGIIWATD GAKVIN+SLGNYADSQFLHDAVKYAYDRDVVLV+A+GN Sbjct: 430 LDGSGSGTTYAVAEGIIWATDHGAKVINMSLGNYADSQFLHDAVKYAYDRDVVLVAASGN 489 Query: 480 DNTERPGYPAAYPEVLAVAATSANGDRASFSNYGDYIDVSAPGESIASTYPDSQYAALSG 539 DNTERPGYPAAYPEVLAVAAT +N RA +SNYGDYIDV+APG +IAST+P +QYAALSG Sbjct: 490 DNTERPGYPAAYPEVLAVAATDSNQKRAEYSNYGDYIDVAAPGTNIASTFPGNQYAALSG 549 Query: 540 TSMASPHVAALAGLVRSLNPDLTNKEVMALMTSNAVDLGDPGQDKYYGWGQVDIYKTLQA 599 TSMASPH AA+AGL+RSLNPDLTN+EVM +M + AVDLGD G+DKYYG GQ+DI+++L+A Sbjct: 550 TSMASPHAAAMAGLIRSLNPDLTNQEVMDVMINQAVDLGDKGRDKYYGHGQIDIFQSLKA 609 Query: 600 AGGGEVPLQLFPQHVGRQLKTLLQSL 625 G EVPLQ++P H R L+ L+ L Sbjct: 610 VGAPEVPLQMWPGHTLRNLEQELRKL 635 >B1DFM8.1 B1DFM8_9BACL Peptidase S8 and S53 subtilisin kexin sedolisin Length = 621 Score = 641 bits (1654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/590 (54%), Positives = 419/590 (71%), Gaps = 15/590 (2%) Query: 42 HQEKTIKKKALTQDVSSTDRLNRTDVSKHLRTMLS-ETDGKTPEDISAYAKQLQQGHGHI 100 HQEKT+K K L +D+ +T L T+ SK R +++ ET T + + L H H+ Sbjct: 43 HQEKTLKVKTLNKDMDATALLCTTECSKEFRKLMNNETKEGTMKAQTDKVAHLMNMHKHM 102 Query: 101 AMLMWIDFRTKQSSTFKSSFPEGSDQENKRLLKYLNTAKTAIQRHQSYESPSFI--IGNK 158 + WI+ + S + + P+ K +Y+ AK I+ Q Y S F+ G++ Sbjct: 103 VYISWINGK---RSVDRGALPD------KTAAQYVEEAKAKIKASQPYHSKPFMSETGSR 153 Query: 159 KYYFTAQRDREGHYGVIALINQRILDRVAEHQLKNLRLIPYPKEGKYRVESVHADTLKEL 218 + GV+ +INQ I+ +V HQ +NLRL+PYP EG YR+ESV +T +E Sbjct: 154 YMVLGVPSGKNNGIGVVGVINQDIVMQVERHQKRNLRLVPYPAEGNYRIESVEPNTNRET 213 Query: 219 TVKTGHDNENASHYYENEIVVRFKNGHPTAGQLQTIAADIRCKEPRKLGYAYIFRSGKLN 278 TV+ G DN NASHY+ ++VV F++ T QL+ I I + +++G Y+FRS ++ Sbjct: 214 TVRDGEDNGNASHYHVKDVVVHFQD-KLTTQQLEQIKQKINADKIQRIGDTYVFRSKNMD 272 Query: 279 YPQLKAYFDQKWNPQYTEPHYMYLTNDTVGENTGAAVITPNDLLFSTYQWNLPAIETEQG 338 ++ YF + W +Y EPHY+YLTN+T +NT A +ITPND L+S YQWNLP+IETE+G Sbjct: 273 ADKMVHYFQKSWKTEYVEPHYLYLTNET-ADNT-ADIITPNDALYSQYQWNLPSIETEKG 330 Query: 339 WNLSKGSKEVIVAVVDTGVQANHPDLKGQLLAGYNAITNTSPPNDDVGHGTHVAGIIGAL 398 W LSKG+ +VIVA++DTGVQA+HPDLKG+LL G N + + PNDDVGHGTHVAGIIGA Sbjct: 331 WGLSKGNDKVIVAILDTGVQADHPDLKGKLLNGTNIVDTNAAPNDDVGHGTHVAGIIGAT 390 Query: 399 TNNGEGVAGISWYNKILPVKALDSSGAGTTYSVAEGIIWATDKGAKVINLSLGNYADSQF 458 NNGEGVAGISWYNKI+PVK LDSSGAG+TYSVA+GIIWATD GAKVIN+SLGNYA ++F Sbjct: 391 VNNGEGVAGISWYNKIMPVKVLDSSGAGSTYSVAQGIIWATDHGAKVINMSLGNYAQAEF 450 Query: 459 LHDAVKYAYDRDVVLVSAAGNDNTERPGYPAAYPEVLAVAATSANGDRASFSNYGDYIDV 518 LHDA+KYAYD DVV+V+A+GNDNT+RPGYPAAYPEV AVAAT A ++ASFSNYGDYIDV Sbjct: 451 LHDAIKYAYDHDVVMVAASGNDNTDRPGYPAAYPEVFAVAATDAAKNKASFSNYGDYIDV 510 Query: 519 SAPGESIASTYPDSQYAALSGTSMASPHVAALAGLVRSLNPDLTNKEVMALMTSNAVDLG 578 +APG+SIASTYP SQYAALSGTSMASPHVAALAGL+RS+NPDL N+EVM +M +A+DLG Sbjct: 511 AAPGDSIASTYPGSQYAALSGTSMASPHVAALAGLIRSINPDLNNEEVMDIMRKSAIDLG 570 Query: 579 DPGQDKYYGWGQVDIYKTLQAAGGGEVPLQLFPQHVGRQLKTLLQSLKNG 628 + G+D YYG+G +D+ K L++A LQ FP R+L+ + + G Sbjct: 571 NKGKDNYYGYGLIDVDKALKSASNYTTALQNFPSQTERRLEAISRRYNTG 620 >C5D2M0.1 C5D2M0_9BACI Peptidase S8 and S53 subtilisin kexin sedolisin Length = 452 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 200/381 (52%), Positives = 267/381 (70%), Gaps = 12/381 (3%) Query: 220 VKTGHDNENASHYYENEIVVRFKNGHPTAGQLQTIAADIRCKEPRKLGYAYIFRSGKLNY 279 +K H+ + SHY+ +EI VRF+ P A +LQ I I K + G +IFRS Y Sbjct: 80 IKIDHNGRDKSHYFNHEITVRFRKL-PAAQELQRIERAIDGKLINQFGRFFIFRSNSKTY 138 Query: 280 PQLKAYFDQKWNPQYTEPHYMYLTNDTVGENTGAAVITPNDLLFSTYQWNLPAIETEQGW 339 +L YF+ Y+EP+Y+YL N+ PNDLL+S YQWNLPAI+TE GW Sbjct: 139 HELHDYFESIPTVSYSEPNYIYLQNEI-----------PNDLLYSRYQWNLPAIDTEAGW 187 Query: 340 NLSKGSKEVIVAVVDTGVQANHPDLKGQLLAGYNAITNTSPPNDDVGHGTHVAGIIGALT 399 LS+G K V +AV+D+G+ +HPDL +L GYN + + + P DD GHGTHVAGII + Sbjct: 188 TLSRGKKGVAIAVIDSGIDLDHPDLVHRLQKGYNVLADNAIPEDDNGHGTHVAGIIASQP 247 Query: 400 NNGEGVAGISWYNKILPVKALDSSGAGTTYSVAEGIIWATDKGAKVINLSLGNYADSQFL 459 NN EGVAGI+W+N I+P+KAL+S G GT++ VA+ I WA D GAKVINLSLGNY S L Sbjct: 248 NNREGVAGITWFNPIMPIKALNSEGYGTSFDVAKAIHWAVDHGAKVINLSLGNYQPSTML 307 Query: 460 HDAVKYAYDRDVVLVSAAGNDNTERPGYPAAYPEVLAVAATSANGDRASFSNYGDYIDVS 519 +A++YAYDRDVVL++A+GND+T +P +PAAYPEV++V A + + A +SNYG Y+DV Sbjct: 308 EEAIRYAYDRDVVLIAASGNDSTAQPSFPAAYPEVISVGAVNPDLSFAHYSNYGTYLDVV 367 Query: 520 APGESIASTYPDSQYAALSGTSMASPHVAALAGLVRSLNPDLTNKEVMALMTSNAVDLGD 579 APG +IAST+ +YAALSGTSMA+PHV ALAGL+RSLNP LTN +V ++ A DLG+ Sbjct: 368 APGTNIASTFSQHRYAALSGTSMAAPHVTALAGLIRSLNPHLTNDDVKQIIIKTATDLGE 427 Query: 580 PGQDKYYGWGQVDIYKTLQAA 600 G+D YYG+G +++Y+ L+ A Sbjct: 428 NGKDPYYGYGLINVYRALELA 448 >Q2BAU2.1 Q2BAU2_9BACI Subtilisin-type proteinase Length = 447 Score = 407 bits (1045), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/383 (52%), Positives = 275/383 (71%), Gaps = 12/383 (3%) Query: 220 VKTGHDN-ENASHYYENEIVVRFKNGHPTAGQLQTIAADIRCKEPRKLGYAYIFRSGKLN 278 VK H N ++ SHY E+E V+F + P +L ++AA + + R + ++F+S + Sbjct: 73 VKIIHHNLKDKSHYIEHEATVKFSSP-PDDDELDSLAAGLDAEFFRHMDSIFVFQSDSMT 131 Query: 279 YPQLKAYFDQKWNPQYTEPHYMYLTNDTVGENTGAAVITPNDLLF-STYQWNLPAIETEQ 337 L +F ++ + Y EP+++ + N+T PNDLL+ YQWNLPAI+ E+ Sbjct: 132 TADLLQFFRERTDVAYAEPNFILMQNETQ---------LPNDLLYREKYQWNLPAIDIEK 182 Query: 338 GWNLSKGSKEVIVAVVDTGVQANHPDLKGQLLAGYNAITNTSPPNDDVGHGTHVAGIIGA 397 W++SKG +++I+AVVDTGV +HPDL+ +L+ GYN + N+S +DD GHGTHVAGII + Sbjct: 183 AWDISKGDEDIIIAVVDTGVDIDHPDLRRRLMKGYNVMQNSSDFDDDNGHGTHVAGIIAS 242 Query: 398 LTNNGEGVAGISWYNKILPVKALDSSGAGTTYSVAEGIIWATDKGAKVINLSLGNYADSQ 457 TNNGEG+AGI+W N+I+PVKA+ S G G T+ +A+GIIWA D GA VINLSLGNY S Sbjct: 243 ETNNGEGIAGITWNNRIMPVKAMGSKGYGYTFDIAKGIIWAADHGADVINLSLGNYQPSA 302 Query: 458 FLHDAVKYAYDRDVVLVSAAGNDNTERPGYPAAYPEVLAVAATSANGDRASFSNYGDYID 517 FL +A++YAYD+ VL+SAAGNDN+E+P +PAA+PEVL+V+A S G++A FSNYG YID Sbjct: 303 FLEEAIRYAYDKGAVLISAAGNDNSEQPSFPAAFPEVLSVSAVSYTGEKADFSNYGYYID 362 Query: 518 VSAPGESIASTYPDSQYAALSGTSMASPHVAALAGLVRSLNPDLTNKEVMALMTSNAVDL 577 V+APG I STY +QYAALSGTSMASPHVA LAGL+ S+NPDL+N+EVM ++ A D+ Sbjct: 363 VTAPGVYIPSTYFKNQYAALSGTSMASPHVAGLAGLILSVNPDLSNREVMNIIKGTARDI 422 Query: 578 GDPGQDKYYGWGQVDIYKTLQAA 600 G+ G+D Y+G G +DI L AA Sbjct: 423 GEEGRDAYFGNGLIDINAALAAA 445 >Q5KZT4.1 Q5KZT4_GEOKA Subtilisin-type proteinase EC=3.4.21.- Length = 453 Score = 390 bits (1003), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/371 (51%), Positives = 263/371 (70%), Gaps = 6/371 (1%) Query: 230 SHYYENEIVVRFKNGHPTAGQLQTIAADIRCKEPRKLGYAYIFRSGKLNYPQLKAYFDQK 289 SHY+ NEIVVRF+ P+ +LQ + A I + +++ + ++FRS + Y +++ YF Sbjct: 87 SHYFANEIVVRFR-ALPSESRLQQMEAAIAGQFIKQVDHVFVFRSREQTYEEMRRYFRSL 145 Query: 290 WNPQYTEPHYMYLTNDTVGENTGAAVITPNDLLFSTYQWNLPAIETEQGWNLSKGSKEVI 349 Y EPHY+Y+ N+ N A V PND ++ YQWNLPAI TE GW LS+G + V Sbjct: 146 PTVDYCEPHYIYMQNEW---NKPAPV--PNDSFYARYQWNLPAIHTEDGWTLSRGKRNVP 200 Query: 350 VAVVDTGVQANHPDLKGQLLAGYNAITNTSPPNDDVGHGTHVAGIIGALTNNGEGVAGIS 409 VA++D+GV HPDL +LL GYN + + PND+ GHGTHVAGII + NNGEGVAG++ Sbjct: 201 VAIIDSGVDLTHPDLTRRLLPGYNVLADDRSPNDENGHGTHVAGIIASQPNNGEGVAGMT 260 Query: 410 WYNKILPVKALDSSGAGTTYSVAEGIIWATDKGAKVINLSLGNYADSQFLHDAVKYAYDR 469 W+N I+ VKAL++ G GT+ VA+GI WA D GAKVINLSLGNY S L +A++YA Sbjct: 261 WFNPIMAVKALNADGYGTSIDVAKGIRWAVDHGAKVINLSLGNYQPSSVLEEAIRYADAH 320 Query: 470 DVVLVSAAGNDNTERPGYPAAYPEVLAVAATSANGDRASFSNYGDYIDVSAPGESIASTY 529 DVVLV+A+GND+T + +PAAYPEV++V A + + A +SNYGDY+DV APG +IAST+ Sbjct: 321 DVVLVAASGNDSTSQASFPAAYPEVISVGAVNPDLSYALYSNYGDYVDVVAPGTNIASTF 380 Query: 530 PDSQYAALSGTSMASPHVAALAGLVRSLNPDLTNKEVMALMTSNAVDLGDPGQDKYYGWG 589 +YAALSGTSMA+PHV ALA L+RS+NP L+N EV ++ +A DLG+ G+D YYG+G Sbjct: 381 AGHRYAALSGTSMAAPHVTALAALIRSVNPRLSNDEVRDIILESADDLGERGKDPYYGYG 440 Query: 590 QVDIYKTLQAA 600 +++Y+ L+ A Sbjct: 441 LINVYRALELA 451 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,985,784,976 Number of extensions: 176156026 Number of successful extensions: 343623 Number of sequences better than 1.0e-05: 3364 Number of HSP's gapped: 335971 Number of HSP's successfully gapped: 3507 Length of query: 629 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 487 Effective length of database: 1,722,912,073 Effective search space: 839058179551 Effective search space used: 839058179551 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)