BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0112.0009.p (393 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG32.1 B3KG32_9BACI Sporulation protein YqfD 460 e-127 B1DFP6.1 B1DFP6_9BACL Sporulation protein YqfD 334 9e-90 C0ZB79.1 C0ZB79_BREBN Putative stage IV sporulation protein 247 1e-63 Q65H67.1 Q65H67_BACLD YqfD 230 2e-58 Q708G6.1 Q708G6_BACLI Sporulation protein 229 5e-58 >B3KG32.1 B3KG32_9BACI Sporulation protein YqfD Length = 397 Score = 460 bits (1183), Expect = e-127, Method: Compositional matrix adjust. Identities = 229/394 (58%), Positives = 281/394 (71%), Gaps = 1/394 (0%) Query: 1 MKQPPLASLRGFVTLHVTGPQVEKFINAVTEADIVIWDVRPAG-DGASLNLLLDDFFALR 59 MK P + LRG++ L V G VE FIN++ EA +WDV P G A LLL DF LR Sbjct: 1 MKIPTMIHLRGYLKLAVRGEHVEAFINSLAEAGTPVWDVTPTGAHSAEFKLLLKDFKGLR 60 Query: 60 PLLKKTGCRTRVTARNGLPFNAAKLWKRKXXXXXXXXXXXXXXXXSSMIWTVQIEGNDRI 119 PLLK+TGCRTR+ R G PF A +L+KRK SSM+W V++EGN+ I Sbjct: 61 PLLKRTGCRTRIKERFGYPFQARRLFKRKAFVIGVVAFFMILLTLSSMVWNVEVEGNETI 120 Query: 120 NSEDILQAARHEGVYPFQWIWRMDDPDKLSKKLTSTLPGVSWVGVQRTGTSVKIHIVESD 179 SED+L AA+ EG+YPFQWI+++ DKLS LT LPG SWVGV+R GT ++I IVE+ Sbjct: 121 ASEDVLDAAKQEGIYPFQWIFKLQSMDKLSGNLTVRLPGTSWVGVERNGTRIRIQIVEAA 180 Query: 180 QPENKPLLSKRHLISRTDAVVTEIYAEQGRPVVTRNTRVKKGQILISGAIGDEENMEYVV 239 PE PL S RHLIS DAV+T +YAE+G P V N+RVKKG ILISG +GDEEN E VV Sbjct: 181 VPEKPPLQSPRHLISTQDAVITHMYAEKGVPKVGINSRVKKGDILISGVLGDEENSERVV 240 Query: 240 AKGEVKGLVWHEYNIEVPLNRTRSSYTGERKDRTYLVLGKWAVQLWGYGKADFAESRTLT 299 AKGEV+GLVWHEY+IE+PL +T YTGE K R+Y+V G AVQLWGYG+ +++ TLT Sbjct: 241 AKGEVRGLVWHEYDIEIPLVKTHKVYTGEGKKRSYIVFGDRAVQLWGYGEIPYSQQETLT 300 Query: 300 EHDPLTWRSFQLPLGLLTEKEMEVLDTAETLTPEAAREEGITRAKNDILARYGTDSVIKS 359 +DPLTWR+ LPLG +TEK MEV + T+T + A++EGI A+ DILA+YGTDS S Sbjct: 301 FYDPLTWRTRVLPLGWMTEKIMEVTEVQRTITEDEAKKEGIEGARRDILAKYGTDSRFVS 360 Query: 360 QKILHEKKENGKVYMKVLFEVEERITQELPIVNN 393 QKILH+KKENGKVYMKVLFEVEE I +ELPIV + Sbjct: 361 QKILHDKKENGKVYMKVLFEVEETIAKELPIVYD 394 >B1DFP6.1 B1DFP6_9BACL Sporulation protein YqfD Length = 397 Score = 334 bits (856), Expect = 9e-90, Method: Compositional matrix adjust. Identities = 172/385 (44%), Positives = 244/385 (63%), Gaps = 1/385 (0%) Query: 9 LRGFVTLHVTGPQVEKFINAVTEADIVIWDVR-PAGDGASLNLLLDDFFALRPLLKKTGC 67 +RG VT+H+ G Q E+ +N E + + +R +G L L DFF LRP LK+TGC Sbjct: 9 VRGVVTVHIRGGQPERLVNRALEGGLELSSIRWTSGGKLEFELSLGDFFRLRPFLKETGC 68 Query: 68 RTRVTARNGLPFNAAKLWKRKXXXXXXXXXXXXXXXXSSMIWTVQIEGNDRINSEDILQA 127 R V R G+PF ++ KRK SS++W+++++GN ++ E IL A Sbjct: 69 RAHVVKREGMPFWLVRVEKRKFFAIGIGLFFVGIYLLSSLVWSIEVKGNVKLTEEQILTA 128 Query: 128 ARHEGVYPFQWIWRMDDPDKLSKKLTSTLPGVSWVGVQRTGTSVKIHIVESDQPENKPLL 187 A+ EG++P QW +++ D D LSK+L + LPG +WVGV++ GT + I +VES + E L Sbjct: 129 AKKEGIFPMQWSFKLKDKDLLSKELVTDLPGTTWVGVEKKGTRIVIQVVESTEAEKAELQ 188 Query: 188 SKRHLISRTDAVVTEIYAEQGRPVVTRNTRVKKGQILISGAIGDEENMEYVVAKGEVKGL 247 S RHL++ DAV+TEIY E+GRPVV +N +VKKGQ LISG IG + VVAKG+V+GL Sbjct: 189 SPRHLVASNDAVITEIYTEKGRPVVKKNMKVKKGQTLISGTIGGGAYTQSVVAKGKVRGL 248 Query: 248 VWHEYNIEVPLNRTRSSYTGERKDRTYLVLGKWAVQLWGYGKADFAESRTLTEHDPLTWR 307 VW+E+N+ PL + YTGE+K + Y V+G A+Q+ GYGK F S + + TWR Sbjct: 249 VWYEFNVASPLTQEVKVYTGEKKTKWYAVIGSRALQVSGYGKNPFDTSENVVVEEQATWR 308 Query: 308 SFQLPLGLLTEKEMEVLDTAETLTPEAAREEGITRAKNDILARYGTDSVIKSQKILHEKK 367 +LP G L + MEV LT E A+ G+ +AK D++ + G D+VI + +LHEK Sbjct: 309 GLKLPFGRLKKTIMEVRSDERELTEEEAKNNGMLQAKADVMLKAGNDAVILKEIVLHEKT 368 Query: 368 ENGKVYMKVLFEVEERITQELPIVN 392 +NGKVYMKVLFEVE+ I +E+P+V Sbjct: 369 DNGKVYMKVLFEVEQSIVEEMPLVQ 393 >C0ZB79.1 C0ZB79_BREBN Putative stage IV sporulation protein Length = 394 Score = 247 bits (631), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 135/384 (35%), Positives = 209/384 (54%), Gaps = 1/384 (0%) Query: 11 GFVTLHVTGPQVEKFINAVTEADIVIWDVRPAGDGAS-LNLLLDDFFALRPLLKKTGCRT 69 G VT+ V G + E+ +N I IW++R G+ + ++L+ D+F LRPLLK+TGCR+ Sbjct: 11 GHVTITVRGKRFERMLNMAVRDGIRIWNIRRVGEEVTRCDILIRDYFRLRPLLKETGCRS 70 Query: 70 RVTARNGLPFNAAKLWKRKXXXXXXXXXXXXXXXXSSMIWTVQIEGNDRINSEDILQAAR 129 V AR+GLPF ++ +R SS +W+V++ G ++ ++QAA Sbjct: 71 HVEARDGLPFWLLRIRRRSGLAIGAALFFIGLYMLSSFVWSVEVSGTKMVDPRRVIQAAE 130 Query: 130 HEGVYPFQWIWRMDDPDKLSKKLTSTLPGVSWVGVQRTGTSVKIHIVESDQPENKPLLSK 189 G+ W ++ +P L K+L S LP SWVG++ GT V + +VE + P Sbjct: 131 QVGIKEGAWKVKLKEPLVLQKELLSLLPEASWVGIEIQGTKVMLQVVEKEAPVKPLATGP 190 Query: 190 RHLISRTDAVVTEIYAEQGRPVVTRNTRVKKGQILISGAIGDEENMEYVVAKGEVKGLVW 249 RHL+++ AVV +I E GR V N V+KGQ+LISG IG+++ + V A+G+V+G VW Sbjct: 191 RHLVAKKRAVVHKILPEVGRSQVVSNQLVEKGQVLISGIIGNDDRQQAVSARGKVQGEVW 250 Query: 250 HEYNIEVPLNRTRSSYTGERKDRTYLVLGKWAVQLWGYGKADFAESRTLTEHDPLTWRSF 309 + N VPL + TGE+ YL++G +AV +W + K FA++ + ++ + Sbjct: 251 YISNTSVPLKQALHRLTGEKLQHQYLLVGPYAVYVWPFEKQSFAQAEMAEQRFLPSYAGY 310 Query: 310 QLPLGLLTEKEMEVLDTAETLTPEAAREEGITRAKNDILARYGTDSVIKSQKILHEKKEN 369 PLG T + ++ E A E A+ DIL + G D+ I+ +K+LH EN Sbjct: 311 TSPLGWKTVTYAQTEPVQTEMSVEEATELAKKFAREDILKKAGKDAFIQDEKVLHVTTEN 370 Query: 370 GKVYMKVLFEVEERITQELPIVNN 393 GKVY+ + F V E I E P V Sbjct: 371 GKVYVSIHFSVIEDIAIEQPFVGQ 394 >Q65H67.1 Q65H67_BACLD YqfD Length = 398 Score = 230 bits (586), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 124/394 (31%), Positives = 208/394 (52%), Gaps = 2/394 (0%) Query: 1 MKQPPLASLRGFVTLHVTGPQVEKFINAVTEADIVIWDVRPAGDGASLNLLLDDFFALRP 60 MK L+ G + L +TG +E+ +N T +I +++V+ D L + L D A R Sbjct: 1 MKNKWLSFFSGKIQLKITGKGIERLLNECTRRNIPMFNVKKKKDAVFLYIPLSDVHAFRK 60 Query: 61 LLKKTGCRTRVTARNGLPFNAAKLWKRKXXXXXXXXXXXXXXXXSSMIWTVQIEGNDRIN 120 +++ C+ R R G PF K + S+M+W + I G + Sbjct: 61 VIRGFDCKCRFIKRKGFPFLVQKSKRNSGFTFGVAAFFIIMFLLSNMLWKIDITGANPET 120 Query: 121 SEDILQAARHEGVYPFQWIWRMDDPDKLSKKLTSTLPGVSWVGVQRTGTSVKIHIVESDQ 180 I Q GV ++ + M P+K+ + LT + ++WVG++ GT++ + +VE ++ Sbjct: 121 EHQIRQQLDQIGVKKGRFQFSMLTPEKIQQALTKRVENITWVGIELNGTALHMKVVEKNE 180 Query: 181 PENKPLLSKRHLISRTDAVVTEIYAEQGRPVVTRNTRVKKGQILISGAIGDEENMEYVVA 240 P+ + + RH++++ A +++++ E+G P+VT N V+KGQ+L+SG IG EE + V A Sbjct: 181 PDKEKYIGPRHIVAKKGATISKMFVEKGEPLVTVNQHVEKGQMLVSGLIGSEEEKQKVGA 240 Query: 241 KGEVKGLVWHEYNIEVPLNRTRSSYTGERKDRTYLVLGKWAVQLWGYG--KADFAESRTL 298 KG++ G W++ + VPL + +TG+ + L LG ++V +WG+ K DF+ +T Sbjct: 241 KGKIYGETWYKSTVTVPLETSFDVFTGKVRTSHKLSLGSFSVPIWGFSFKKEDFSRPKTE 300 Query: 299 TEHDPLTWRSFQLPLGLLTEKEMEVLDTAETLTPEAAREEGITRAKNDILARYGTDSVIK 358 TE L + +F+LP+ E E + + A EGI K DI + G D I Sbjct: 301 TEKHSLHFMNFKLPVAYEKEHMRESEQIKRVYSKKEAVLEGIEMGKRDIRKKIGRDGNII 360 Query: 359 SQKILHEKKENGKVYMKVLFEVEERITQELPIVN 392 S+K+LHE ENGKV + +L++V E I Q PIV Sbjct: 361 SEKVLHETSENGKVKLIILYQVIENIVQTTPIVQ 394 >Q708G6.1 Q708G6_BACLI Sporulation protein Length = 398 Score = 229 bits (583), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 124/394 (31%), Positives = 209/394 (53%), Gaps = 2/394 (0%) Query: 1 MKQPPLASLRGFVTLHVTGPQVEKFINAVTEADIVIWDVRPAGDGASLNLLLDDFFALRP 60 MK L+ G + L +TG +E+ +N T +I +++V+ D L + L D A R Sbjct: 1 MKNKWLSFFSGKIQLKITGKGIERLLNECTRRNIPMFNVKKKKDAVFLYIPLSDVHAFRK 60 Query: 61 LLKKTGCRTRVTARNGLPFNAAKLWKRKXXXXXXXXXXXXXXXXSSMIWTVQIEGNDRIN 120 +++ C+ R R G PF K + S+M+W + I G + Sbjct: 61 VIRGFDCKCRFIKRKGFPFLVQKSKRNSGFTFGVAAFFIIMLLLSNMLWKIDITGANPET 120 Query: 121 SEDILQAARHEGVYPFQWIWRMDDPDKLSKKLTSTLPGVSWVGVQRTGTSVKIHIVESDQ 180 I Q GV ++ + M P+K+ + LT + ++WVG++ GT++ + +VE ++ Sbjct: 121 EHQIKQQLDQIGVKKGRFQFSMLTPEKIQQALTKRVENITWVGIELNGTALHMKVVEKNE 180 Query: 181 PENKPLLSKRHLISRTDAVVTEIYAEQGRPVVTRNTRVKKGQILISGAIGDEENMEYVVA 240 P+ + + RH++++ A +++ + E+G P+VT N V+KGQ+L+SG IG EE + V A Sbjct: 181 PDKEKYIGPRHIVAKKGATISKKFVEKGEPLVTVNQHVEKGQMLVSGLIGSEEEKQKVGA 240 Query: 241 KGEVKGLVWHEYNIEVPLNRTRSSYTGERKDRTYLVLGKWAVQLWGYG--KADFAESRTL 298 KG++ G W++ + VPL + +TG+ + L LG ++V +WG+ K DF+ +T Sbjct: 241 KGKIYGETWYKSTVTVPLETSFDVFTGKVRTSHKLSLGSFSVPIWGFSFKKEDFSRPKTE 300 Query: 299 TEHDPLTWRSFQLPLGLLTEKEMEVLDTAETLTPEAAREEGITRAKNDILARYGTDSVIK 358 TE+ L + +F+LP+ E E + + A EGI K DI + G+D I Sbjct: 301 TENPSLHFMNFKLPVAYEKEHMRESEQIKRVYSKKEAVLEGIEMGKRDIRKKIGSDGNII 360 Query: 359 SQKILHEKKENGKVYMKVLFEVEERITQELPIVN 392 S+K+LHE ENGKV + +L++V E I Q PIV Sbjct: 361 SEKVLHETSENGKVKLIILYQVIEDIVQTTPIVQ 394 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,302,357,978 Number of extensions: 93310255 Number of successful extensions: 215580 Number of sequences better than 1.0e-05: 190 Number of HSP's gapped: 215118 Number of HSP's successfully gapped: 190 Length of query: 393 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 255 Effective length of database: 1,760,596,133 Effective search space: 448952013915 Effective search space used: 448952013915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)