BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0010.p (542 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKN7.1 B3KKN7_9BACI Polysaccharide biosynthesis protein 622 e-176 B1DJ30.1 B1DJ30_9BACL Polysaccharide biosynthesis protein 420 e-115 A6CR25.1 A6CR25_9BACI YabM 169 5e-40 C2QIJ0.1 C2QIJ0_BACCE Export protein for polysaccharides and tei... 169 8e-40 A3IFU3.1 A3IFU3_9BACI Polysaccharides export protein 164 2e-38 >B3KKN7.1 B3KKN7_9BACI Polysaccharide biosynthesis protein Length = 541 Score = 622 bits (1605), Expect = e-176, Method: Compositional matrix adjust. Identities = 319/540 (59%), Positives = 397/540 (73%), Gaps = 2/540 (0%) Query: 3 TKKESFVKGTXXXXXXXXXXXXXXXXQRVPLEHLFNDIGNASFTQANNVYLMLLPLATAG 62 +KKESF+KGT QRVPLEH+ NDIG+ASFT ANNVYLMLL +ATAG Sbjct: 2 SKKESFIKGTIILAAAALVARVLGLAQRVPLEHMLNDIGDASFTIANNVYLMLLTVATAG 61 Query: 63 IPSTLSKMVSERYALNRPHEAQQVYRAALIFAAVVGVLMSTILYIAAPYYAEY-SKVPES 121 IPSTLSKMVSER+ALN+P EA++VY AALIFA GV+M+ +LY AP+YA + +K PE+ Sbjct: 62 IPSTLSKMVSERHALNKPAEARRVYHAALIFAGAAGVVMTLLLYFGAPFYATHIAKQPEA 121 Query: 122 TXXXXXXXXXXXXFPMIAMMRGYFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGY 181 FP IAMMRGYFQGRNNM AGGISQIIEQIARV TAI LA++LLR GY Sbjct: 122 AAAIQALAPALLLFPAIAMMRGYFQGRNNMTAGGISQIIEQIARVLTAIGLAYVLLRLGY 181 Query: 182 SNTWMAAGASFGSVLGSIGAFGVMLYYAVKLRRSEEQKALYDSSQARIPLLKIYKDIFKL 241 +TW+AAGASFG VLGSIGAF VMLY+ VKLRR++ + + + ++ IPL IY+DIF L Sbjct: 182 DDTWIAAGASFGGVLGSIGAFAVMLYFTVKLRRNDRELKMPAADRS-IPLGGIYRDIFTL 240 Query: 242 SIPIVLSSVTVPVVNFIDTSFIVPLLSGQIGREAATQALGIFGSRAQSVAGIPPVLSIAL 301 SIPIVLSS+ VP VNFID+S + PLL GQ+G AT AL + G+RAQSVAGIPP+L+IAL Sbjct: 241 SIPIVLSSLAVPAVNFIDSSIVKPLLIGQVGLGEATAALAVLGNRAQSVAGIPPILAIAL 300 Query: 302 STSLIPVISAAYARREEAHLKRQITLALRISILTGTPIVLSLVVAAYSVNGLLFSSLDGS 361 STSLIP+ISAA+AR+++ HL++Q++LA+R++ILTG P+V++L VA+YS+NGLLFSSL GS Sbjct: 301 STSLIPIISAAFARKDQEHLEQQVSLAMRVAILTGMPMVIALCVASYSINGLLFSSLGGS 360 Query: 362 GIVAMLTLGTIFQITMMTTNSILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFGIYGII 421 GI+ LT GTIFQITMMTTNSILLG+GKA++SM +V+ G+ VK ++FLL+ LFGIYGII Sbjct: 361 GIIGFLTFGTIFQITMMTTNSILLGIGKAKLSMVHVMIGIAVKLVASFLLAPLFGIYGII 420 Query: 422 AATALCFIVITLLNLRMLKTIVPFEILGKRWXXXXXXXXXXXXXXXXXNEAGQLLTHMMP 481 +TALCF+VIT+LN+R +K IV F ILGKRW N AG + +MP Sbjct: 421 GSTALCFLVITMLNVRSIKAIVSFSILGKRWMGFILTVAAAGGIGYGLNLAGIQMVDVMP 480 Query: 482 VRLAFLITCXXXXXXXXXXXXXXXXXXXXXSRQEIAGYPRPLQKLLGPLMKLQPERVRSE 541 RLAFL+TC + E++ YPRPLQK+ PLM+LQP R+R Sbjct: 481 ARLAFLLTCLVVGVAVVIIYLVLLVVFGVLRQSELSSYPRPLQKVFRPLMRLQPARMRER 540 >B1DJ30.1 B1DJ30_9BACL Polysaccharide biosynthesis protein Length = 545 Score = 420 bits (1080), Expect = e-115, Method: Compositional matrix adjust. Identities = 220/455 (48%), Positives = 300/455 (65%), Gaps = 6/455 (1%) Query: 4 KKESFVKGTXXXXXXXXXXXXXXXXQRVPLEHLFNDIGNASFTQANNVYLMLLPLATAGI 63 KK+S +KGT QRVPL+H+ + G F ANNVYL LL +AT G+ Sbjct: 2 KKDSLIKGTLILAAAALIARFLGIFQRVPLDHILTENGGLYFAVANNVYLTLLVVATGGL 61 Query: 64 PSTLSKMVSERYALNRPHEAQQVYRAALIFAAVVGVLMSTILYIAAPYYAEY-SKVPEST 122 PS +SKMVSERYAL R HEA++VY+AAL+F + GV+++ LYIAAPYYA + +K P + Sbjct: 62 PSAISKMVSERYALGREHEAKRVYQAALLFGVITGVILTIALYIAAPYYAVHIAKTPGAE 121 Query: 123 XXXXXXXXXXXXFPMIAMMRGYFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYS 182 FP+IAMMRGYFQGR MMAGG+SQI+EQI RV + LA+ ++ + Sbjct: 122 VAIRAVAPTLLIFPIIAMMRGYFQGRQFMMAGGMSQIVEQILRVIVGVALAYYVVSIDWG 181 Query: 183 NTWMAAGASFGSVLGSIGAFGVMLYYAVKLRRSE-EQKALYD----SSQARIPLLKIYKD 237 W AA ASFG+V+G +GA VMLYYA KLRR + + D + A+IP IY++ Sbjct: 182 EKWGAAAASFGNVIGGVGALLVMLYYAAKLRRGDLVDRPSVDIEAVKAAAKIPFRSIYRE 241 Query: 238 IFKLSIPIVLSSVTVPVVNFIDTSFIVPLLSGQIGREAATQALGIFGSRAQSVAGIPPVL 297 IF +SIPIV++++TV ++ D+S V L G A L +AQ+VAGIPP+L Sbjct: 242 IFTMSIPIVITAMTVQIIYTFDSSLFVRLTESYYGYHDALLVLRDLSFKAQTVAGIPPIL 301 Query: 298 SIALSTSLIPVISAAYARREEAHLKRQITLALRISILTGTPIVLSLVVAAYSVNGLLFSS 357 +IALSTS+IPVIS+AY+ R A ++RQ +L +RI + TG P L+L A+YSV GL+F+ Sbjct: 302 AIALSTSIIPVISSAYSLRNMAEVERQTSLVMRIVLFTGVPAALTLAAASYSVTGLIFTG 361 Query: 358 LDGSGIVAMLTLGTIFQITMMTTNSILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFGI 417 G GIVA LT GTIFQITMMT+NSIL G+GKARI M G+ +K ++ L+ ++G+ Sbjct: 362 PRGYGIVAALTAGTIFQITMMTSNSILFGLGKARIPMMNTFVGIGLKILCSYALAPIYGV 421 Query: 418 YGIIAATALCFIVITLLNLRMLKTIVPFEILGKRW 452 YG+I AT++CF+V+T +N+ ++ V F +LG RW Sbjct: 422 YGVIMATSVCFVVMTWMNIEAIRREVKFTVLGSRW 456 >A6CR25.1 A6CR25_9BACI YabM Length = 522 Score = 169 bits (429), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 126/418 (30%), Positives = 206/418 (49%), Gaps = 17/418 (4%) Query: 30 RVPLEHLFNDIGNASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRA 89 RVP +++ DIG + Q Y + L L+T G P +SKM++ER E Q V R+ Sbjct: 22 RVPFQNIVGDIGFYIYQQVYPFYGIALALSTYGFPVIISKMIAERQEGKNDKEVQAVLRS 81 Query: 90 ALIFAAVVGVLMSTILYIAAPYYAEYSKVPESTXXXXXXXXXXXXFPMIAMMRGYFQGRN 149 + +F +V GV+ ++Y A A P+ P I+ +RG++QG Sbjct: 82 SFLFLSVTGVVWFVLIYFGAGPIASLMGDPQLQGLIELISVTFLLLPFISGLRGFYQGAG 141 Query: 150 NMMAGGISQIIEQIARVSTAILLAFILLRQGYSNTWMAAGASFGSVLGSIGAFGVMLYYA 209 NM+ SQ+ EQ+ RV T I + IL QGYS AGA FGS+ G + +++ + Sbjct: 142 NMVPTAYSQVGEQLVRVVTIISFSAILTAQGYSLYMAGAGALFGSITGGLACIVILVTFL 201 Query: 210 VKLRRSEEQKALYDSSQARIPLLK------IYKDIFKLSIPIVLSSVTVPVVNFIDTSFI 263 K ++ + L P++K I+ +FK I +S + + ++ ID + Sbjct: 202 WK----DKNRLLRP-----FPIIKGGGHREIWSRLFKHGTAICVSGMLLVLLQLIDALNV 252 Query: 264 VPLL-SGQIGREAATQALGIFGSRAQSVAGIPPVLSIALSTSLIPVISAAYARREEAHLK 322 LL + + A Q GI+ R Q + V+S +LS +L+P+I+AA+ + E + + Sbjct: 253 YSLLLNSGVSENTAKQLKGIY-DRGQPFIQLGTVVSTSLSLTLVPLITAAFRKGETSEMN 311 Query: 323 RQITLALRISILTGTPIVLSLVVAAYSVNGLLFSSLDGSGIVAMLTLGTIFQITMMTTNS 382 ++I LAL+I L G L L+ N +LF + GS ++A+ L +F ++T ++ Sbjct: 312 KKIRLALKIGSLAGFAASLGLINIIKPTNAMLFQNSAGSDVIAVFCLSILFSSVILTISA 371 Query: 383 ILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFGIYGIIAATALCFIVITLLNLRMLK 440 IL G+ +L GL VKFG N LL +G G +T + +I L+ + LK Sbjct: 372 ILQGIELIYFPALTILGGLAVKFGLNILLVSRYGTMGASISTVIALGIIALILVVRLK 429 >C2QIJ0.1 C2QIJ0_BACCE Export protein for polysaccharides and teichoic acids Length = 550 Score = 169 bits (427), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 122/433 (28%), Positives = 218/433 (50%), Gaps = 13/433 (3%) Query: 32 PLEHLFNDIGNASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRAAL 91 P L G +T Y + L +ATAG+P +SK VS+ AL +++++R+ + Sbjct: 30 PFHALVGTEGGTLYTYGYIPYTIFLSIATAGVPLAVSKFVSKYNALGDYKTSRRMFRSGM 89 Query: 92 IFAAVVGVLMSTILYIAAPYYAE--------YSKVPESTXXXXXXXXXXXXFPMIAMMRG 143 + V GVL +LY+ AP +AE +S V E T P +++RG Sbjct: 90 VMMIVTGVLSFLVLYMTAPLFAEAMLGKQSVHSNVGEVTTIIRLVSFALIVVPAASLIRG 149 Query: 144 YFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYSNTWMAAG-ASFGSVLGSIGAF 202 YFQG +M +SQIIEQI R+ + +FI+++ A G A+F + + ++GA Sbjct: 150 YFQGHQSMGPTTVSQIIEQIIRIVFLLAGSFIVIKVLGGTVATAVGVATFAAFVSAVGAL 209 Query: 203 GVMLYYAVKLRRSEEQKALYDS-SQARIPLLKIYKDIFKLSIPIVLSSVTVPVVNFIDTS 261 GV+++Y +K ++ +Q + + ++ + ++++K++F +IP V+ +T+P+ IDT Sbjct: 210 GVLIWYWLKRKKHLDQYLIEQTVPESTVSTVQLFKELFAYAIPYVVIGLTIPLYQQIDTL 269 Query: 262 FIVPLLSGQIGREAATQALGIFGSRAQSVAGIPPVLSIALSTSLIPVISAAYARREEAHL 321 ++ + A +ALGIF + IP L+ A S +L+P I+ ++ ++ +L Sbjct: 270 TFNSIMQAIGQGDIAERALGIFTMWTHKLIMIPVSLATAFSLTLVPAITKSFTEKQHRYL 329 Query: 322 KRQITLALRISILTGTPIVLSLVVAAYSVNGLLFSSLDGSGIVAMLTLGTIFQITMMT-T 380 K QIT + ++ P V+ + AY + + S G V M + T T Sbjct: 330 KLQITQTFQANMFLTLPAVIGISTLAYPIYTAFYDSDPLGGQVLMWYAPVALLFALFTVT 389 Query: 381 NSILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFGIYGIIAATALCFIVITLLNLRMLK 440 +IL G+ + + ++ ++ G+++KF N + R FG G I ATA+ F+ R +K Sbjct: 390 AAILQGINQQKHAIVALIMGVILKFVCNVIFIRYFGTVGAILATAVGFLASVWYTNRQIK 449 Query: 441 TIV--PFEILGKR 451 V F ++ KR Sbjct: 450 KHVHYSFGVVYKR 462 >A3IFU3.1 A3IFU3_9BACI Polysaccharides export protein Length = 518 Score = 164 bits (415), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 130/448 (29%), Positives = 222/448 (49%), Gaps = 8/448 (1%) Query: 5 KESFVKGTXXXXXXXXXXXXXXXXQRVPLEHLFNDIGNASFTQANNVYLMLLPLATAGIP 64 + +FVK T R+PL+++ D F+ VY++ L L+ AGIP Sbjct: 3 QSTFVKSTLVLTIATLLSKVIGSIFRIPLQNIAGDEVLGIFSLVYPVYMVALYLSVAGIP 62 Query: 65 STLSKMVSERYALNRPHEAQQVYRAALIFAAVVGVLMSTILYIAAPYYAEYSKVPESTXX 124 +SK+++E + + +++Y A I A GV+ TI+Y + A P + Sbjct: 63 IAISKLIAEANSSKDTAKIKKIYVTARILALSFGVISFTIIYGFSAQIANALGGPSTEIA 122 Query: 125 XXXXXXXXXXFPMIAMMRGYFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYSNT 184 P +A+ RG+FQG +M ISQ+IEQ+ RV+ +++A+ L+ YSN Sbjct: 123 LIIVALTLLVAPYMAVYRGFFQGFGDMRPTAISQVIEQLVRVALILIVAYFLVAMDYSND 182 Query: 185 WMAAGASFGSVLGSIGAFGVMLYYAVKLRRSEEQKALYDSSQARIPLLKIYKDIFKLSIP 244 +A G GSVLG++G+ +LY K RS + D ++ K I K+SIP Sbjct: 183 IVAGGIMAGSVLGALGSLVYLLY---KYFRSSVKVTSKDKYSSK-DFSTYSKTILKISIP 238 Query: 245 IVLSSVTVPVVNFIDTSFIVPLLSGQIGREAATQAL--GIFGSRAQSVAGIPPVLSIALS 302 I + SVT+ + NF+D SF V G +A+ + GI+G R ++ I V + ++ Sbjct: 239 IAIGSVTMALFNFVD-SFTVTYGLRNAGADASEISFLYGIYG-RGLTLVQITTVFASSII 296 Query: 303 TSLIPVISAAYARREEAHLKRQITLALRISILTGTPIVLSLVVAAYSVNGLLFSSLDGSG 362 L+P+I+ A R+ + I R++ L P + L+ +N LF L+GS Sbjct: 297 LPLVPLITTKIAERKMDETRSIIERTHRMTHLISWPAAIGLLALTLPLNLALFKDLEGSL 356 Query: 363 IVAMLTLGTIFQITMMTTNSILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFGIYGIIA 422 ++AM+ L ++F + +L GM A+ +L+G+++K +N + FG+ G Sbjct: 357 MLAMINLSSVFTSLTLLGTGVLQGMNAAKTGALIILSGVVLKVFANIFFIQAFGLDGAAY 416 Query: 423 ATALCFIVITLLNLRMLKTIVPFEILGK 450 AT L + V+ ++N + ++ F +LGK Sbjct: 417 ATLLVYFVLFIVNTVFIYRMIRFTVLGK 444 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.138 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,398,440,729 Number of extensions: 83370047 Number of successful extensions: 343411 Number of sequences better than 1.0e-05: 1021 Number of HSP's gapped: 340610 Number of HSP's successfully gapped: 1021 Length of query: 542 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 401 Effective length of database: 1,732,333,088 Effective search space: 694665568288 Effective search space used: 694665568288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)