BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0008.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKN9.1 B3KKN9_9BACI Cof-like hydrolase 346 1e-93 B1DJ33.1 B1DJ33_9BACL Cof-like hydrolase 297 6e-79 Q9KFI2.1 Q9KFI2_BACHD BH0497 protein 234 7e-60 B3K8K9.1 B3K8K9_9BACI Cof-like hydrolase 232 3e-59 Q2B9C9.1 Q2B9C9_9BACI YcsE 228 3e-58 >B3KKN9.1 B3KKN9_9BACI Cof-like hydrolase Length = 248 Score = 346 bits (888), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 160/244 (65%), Positives = 200/244 (81%) Query: 6 MIAKYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLG 65 M KYRLLALDMDGTLL D+ +++ +TV+WIK+A+ G+HVCLSTGR + A PYA+QL Sbjct: 1 MKHKYRLLALDMDGTLLQDDHEVSEETVKWIKEAVRHGIHVCLSTGRPFQDAYPYAKQLE 60 Query: 66 LETPMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNWD 125 L TPM+ VNG EVWR P EL+RR+LLD LV+ MH +A E G+WFWA SVD +YN +NW Sbjct: 61 LTTPMVTVNGGEVWRAPYELHRRALLDSKLVEQMHALAVETGSWFWAYSVDRLYNSENWT 120 Query: 126 GDIEGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIREV 185 D+ +EWLKFGY+T D ++RH ++MRLQD+GGLEITNSSP NLEINP G+NKA+GI +V Sbjct: 121 DDVANQEWLKFGYNTNDHEVRHNIMMRLQDMGGLEITNSSPDNLEINPQGVNKATGIGQV 180 Query: 186 CKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEVI 245 C+LLG+ MS+VVAVGDSLNDLA IQQ+G GVAMGNAQ+ VK EAD VV++NN+DGI EVI Sbjct: 181 CELLGISMSEVVAVGDSLNDLAVIQQAGLGVAMGNAQQTVKDEADLVVSSNNEDGIVEVI 240 Query: 246 QKYI 249 + +I Sbjct: 241 RDHI 244 >B1DJ33.1 B1DJ33_9BACL Cof-like hydrolase Length = 250 Score = 297 bits (761), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 140/247 (56%), Positives = 186/247 (75%), Gaps = 4/247 (1%) Query: 7 IAKYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGL 66 + Y+L+ALDMDGTLLND+ +I+ + EWI++A+DAG+ V STGR RGA+P+AEQL L Sbjct: 1 MGNYKLVALDMDGTLLNDQSEISEENAEWIQRALDAGITVSFSTGRGFRGALPFAEQLKL 60 Query: 67 ETPMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEY-GTWFWACSVDEVYNRDNW- 124 ETPMI NGSE+W+ P L++R+LL VK +HE+A ++ GTWFWA S +YN + W Sbjct: 61 ETPMITANGSEIWQRPHVLHKRTLLSPVYVKQLHELALKHEGTWFWAYSTTGIYNLEKWI 120 Query: 125 --DGDIEGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGI 182 E WLKFGY TEDD IR+++L + + LEITNSS NLE+NP GI KAS + Sbjct: 121 DPSTTYEDHHWLKFGYYTEDDVIRNRILAEITEWDALEITNSSTQNLELNPKGITKASAL 180 Query: 183 REVCKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIA 242 RE+C +LG+EMSQVVA GDSLND+AAI+++G GVAMGNAQ+ VK ADAV TNN++G+A Sbjct: 181 RELCTMLGIEMSQVVAAGDSLNDIAAIREAGLGVAMGNAQQAVKDAADAVTLTNNENGVA 240 Query: 243 EVIQKYI 249 E+I++Y+ Sbjct: 241 EIIKQYV 247 >Q9KFI2.1 Q9KFI2_BACHD BH0497 protein Length = 247 Score = 234 bits (596), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 117/244 (47%), Positives = 167/244 (68%), Gaps = 1/244 (0%) Query: 6 MIAKYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLG 65 M Y+++ALDMDGTLL+ QI+ I++A + GVHV LSTGR+ + Y E L Sbjct: 1 MGGPYKIIALDMDGTLLSSNHQISEGNRRAIEEARNHGVHVILSTGRSFMTSKEYMESLA 60 Query: 66 LETPMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNWD 125 L++ I VNGSE+W T EL R +L ++TM ++ E++ T FWA S ++V+ R + Sbjct: 61 LDSYHITVNGSEIWHTSGELIERQMLHGQHIETMWKLKEQHNTTFWAVSTEKVW-RGEFP 119 Query: 126 GDIEGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIREV 185 +I EWLKFGY + +IR ++ LQ LE+TNSSPTNLEIN +G++KA + V Sbjct: 120 ENIHEHEWLKFGYDVPNQEIREQIEKELQAHSELEVTNSSPTNLEINAMGVSKAKALATV 179 Query: 186 CKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEVI 245 C+ LGL M+ V+A+GDSLNDLA I+++G GVAMGNAQE+VK+ AD V TN++DG+A+ I Sbjct: 180 CERLGLTMANVLAMGDSLNDLAMIEEAGCGVAMGNAQEIVKEAADWVTGTNDEDGVAQAI 239 Query: 246 QKYI 249 +K++ Sbjct: 240 RKFV 243 >B3K8K9.1 B3K8K9_9BACI Cof-like hydrolase Length = 254 Score = 232 bits (591), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 110/245 (44%), Positives = 165/245 (67%) Query: 5 QMIAKYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQL 64 Q+ AKY+L+ALD+DGTLL DE+ IT +T +W++ A+D GV V STGR + A + ++L Sbjct: 6 QVKAKYKLIALDLDGTLLTDEKHITDETKKWLQYAVDQGVKVMFSTGRGLQTAKGFWDEL 65 Query: 65 GLETPMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNW 124 GL++PM+++NG+EVW P L +R L V+ MH IA + G W+W SV+ + +W Sbjct: 66 GLDSPMVLLNGAEVWEGPGRLKKRVFLPRDAVRDMHAIAAKRGEWYWGYSVESLTGDKDW 125 Query: 125 DGDIEGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIRE 184 ++ R+W+KFG + D ++ L G LE+T+S+PTN+EI+ GI K SG+RE Sbjct: 126 TPEMFERDWMKFGIGSNDQRKLAEIKEELLSWGTLEVTHSAPTNMEISVKGITKESGVRE 185 Query: 185 VCKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEV 244 VC+ LG MS V+A+GDS ND ++ +G GVAM N +E +K AD + ATNN++G+A+ Sbjct: 186 VCQTLGFSMSDVIAMGDSDNDAKLLKAAGLGVAMANGEEHIKSIADVITATNNENGVAQA 245 Query: 245 IQKYI 249 I+KY+ Sbjct: 246 IRKYV 250 >Q2B9C9.1 Q2B9C9_9BACI YcsE Length = 254 Score = 228 bits (582), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 113/240 (47%), Positives = 169/240 (70%), Gaps = 1/240 (0%) Query: 9 KYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGLET 68 + +L+ALDMDGTLLN++ +++ + IK+A++AG+ V LSTGRT +A+ L L++ Sbjct: 9 EIKLIALDMDGTLLNEKGEVSDGNRKAIKEAIEAGIKVVLSTGRTRLTCRDHADSLKLDS 68 Query: 69 PMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNWDGDI 128 ++ +NG E+W L R+ + L+ M ++++E+ FWA + ++V+ RD D+ Sbjct: 69 YLVTINGGEIWGPDGSLVERNPVSAELISWMWDLSKEHKAGFWAVNSEKVF-RDEMPEDL 127 Query: 129 EGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIREVCKL 188 EWLKFG++ EDD R +L L + G LEITNS+ TN+E+N LG+NKA+GI+ VC+L Sbjct: 128 LSNEWLKFGFNIEDDAARETVLQTLAERGELEITNSALTNIEVNALGVNKANGIKRVCEL 187 Query: 189 LGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEVIQKY 248 LGL M QV+AVGDSLND++ I++ G+GVAMGNAQE VK+ ADAV A N +DG+A I+K+ Sbjct: 188 LGLTMDQVMAVGDSLNDISMIRECGWGVAMGNAQETVKEAADAVTAANTEDGVAAAIRKF 247 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,543,185,579 Number of extensions: 62160478 Number of successful extensions: 187489 Number of sequences better than 1.0e-05: 3340 Number of HSP's gapped: 186577 Number of HSP's successfully gapped: 4033 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)