BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0003.p (395 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKP5.1 B3KKP5_9BACI Major facilitator superfamily MFS_1 320 2e-85 B1DJ41.1 B1DJ41_9BACL Major facilitator superfamily MFS_1 266 4e-69 C0ZBN2.1 C0ZBN2_BREBN Hypothetical membrane protein 122 4e-26 C3HDV3.1 C3HDV3_BACTU Multidrug efflux transporter 71 2e-10 Q6HNE1.1 Q6HNE1_BACHK Probable multidrug efflux transporter 71 2e-10 >B3KKP5.1 B3KKP5_9BACI Major facilitator superfamily MFS_1 Length = 350 Score = 320 bits (819), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 176/328 (53%), Positives = 214/328 (65%), Gaps = 14/328 (4%) Query: 26 QYPILTPFAISLGAGPAFIGWMMGMYSLTHLPGNLLAGVLXXXPTAPRRYIVFALTAAGA 85 QYP+LTPFAISLGA P FIGWMMG+Y+LTHLPGNLLAG L RRYI+F+LTAAG Sbjct: 21 QYPVLTPFAISLGAAPTFIGWMMGIYALTHLPGNLLAGSLIDR-HGSRRYIIFSLTAAGI 79 Query: 86 ILLMQAHAQLPWHLLLLRAASGFVLAFXXXXXXXXXXXXXXXXXTQGKYMSGHGIVHTLA 145 ILL+QAH QLPW LL+LR+ SGFVLAF TQGKYMSGHG++HTLA Sbjct: 80 ILLLQAHVQLPWQLLVLRSISGFVLAFLSPACLALLASLSRDPVTQGKYMSGHGVIHTLA 139 Query: 146 SVVSPAAGAFIVAKAGYSGTFTTLGWLLILTGVMALFSVPKQVQSMSAAKPALPLHSPAQ 205 SV+SPAAGAFIVA AG+SGTF +LGWLLI TG+MA S+PK MSAA H Q Sbjct: 140 SVLSPAAGAFIVANAGFSGTFQSLGWLLIFTGLMAFLSLPK---PMSAAAK----HKDQQ 192 Query: 206 AADRENPRVSR-----RYYLLPFFVSCSQGVLFFELPLSQ-GSEGMISTGIXXXXXXXXX 259 A N + +R R YL+PF ++CSQG+LFFE+PL + G ++STG+ Sbjct: 193 AEPINNEKPARLPFSLRLYLIPFAIACSQGILFFEIPLRESGLASIMSTGLLFSVISLGA 252 Query: 260 XXXXXXXXXNRFAPGIRIXXXXXXXXXXXXXXXXXRHIPAGVVLFLLGAAKGVLFPAMAS 319 NR+AP IR+ H+P ++LF LG AKG+LFPA++S Sbjct: 253 LVTLSMLFLNRYAPHIRVTIGVFGLAICFYALAAADHVPIMIILFTLGLAKGLLFPALSS 312 Query: 320 LFISLGGPGRMGRTFSLQSIAMSLGAFA 347 LFI L G GR+G+ FS QSIAMSLG+ + Sbjct: 313 LFIQLSGGGRLGKVFSFQSIAMSLGSLS 340 >B1DJ41.1 B1DJ41_9BACL Major facilitator superfamily MFS_1 Length = 386 Score = 266 bits (679), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 154/353 (43%), Positives = 213/353 (60%), Gaps = 4/353 (1%) Query: 26 QYPILTPFAISLGAGPAFIGWMMGMYSLTHLPGNLLAGVLXXXPTAPRRYIVFALTAAGA 85 QYPILTPFAISLGA P+FIG MMGMYS+THLPGN++AG R +IVF+L AG Sbjct: 21 QYPILTPFAISLGAAPSFIGLMMGMYSITHLPGNIMAGY-GVDRYGSRLFIVFSLMGAGV 79 Query: 86 ILLMQAHAQLPWHLLLLRAASGFVLAFXXXXXXXXXXXXXXXXXTQGKYMSGHGIVHTLA 145 +LL Q+ PW LL+LR+ SGFVLAF QGK+M+G+G+VHTLA Sbjct: 80 VLLFQSKVTDPWQLLVLRSISGFVLAFLSPACLALLARISSDVTKQGKFMAGNGLVHTLA 139 Query: 146 SVVSPAAGAFIVAKAGYSGTFTTLGWLLILTGVMALFSVPKQVQSMSAAKPALPLHSPAQ 205 +VVSPAAGA++VA+ G++ F LGW+LI+T V ALF + + +++ A + P S A+ Sbjct: 140 TVVSPAAGAYLVARIGFTTAFYLLGWVLIITAVCALFFI-RDIKAAPAVEVE-PAPSSAK 197 Query: 206 AADRENPRVSRRYYLLPFFVSCSQGVLFFELPL-SQGSEGMISTGIXXXXXXXXXXXXXX 264 E + +LLP +SC+QG+L FELPL + +EGM+ TG+ Sbjct: 198 PVLAEGTPIPWLVFLLPIAMSCAQGILSFELPLMATSAEGMMETGLLFSVVSIGALITLS 257 Query: 265 XXXXNRFAPGIRIXXXXXXXXXXXXXXXXXRHIPAGVVLFLLGAAKGVLFPAMASLFISL 324 N+ + R +P ++L +G AKG++ PA+++L I L Sbjct: 258 MLFLNKLSSYYRTLWGALGLALVYFTIAAQTPLPFFMLLLFVGMAKGIVLPAISTLLIEL 317 Query: 325 GGPGRMGRTFSLQSIAMSLGAFAGPVAAGQLRDFVSPYFIAFVLLMTAILLLP 377 G + GRTFS+ SIA S+GAF GP+ AGQLRD +SPY++AF+ LM + +LP Sbjct: 318 SGGAKYGRTFSVLSIAYSVGAFLGPMLAGQLRDNLSPYYLAFLGLMIGVCVLP 370 >C0ZBN2.1 C0ZBN2_BREBN Hypothetical membrane protein Length = 382 Score = 122 bits (307), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 100/344 (29%), Positives = 154/344 (44%), Gaps = 5/344 (1%) Query: 26 QYPILTPFAISLGAGPAFIGWMMGMYSLTHLPGNLLAG-VLXXXPTAPRRYIVFALTAAG 84 Q P+L P+ LG A IG+++G Y++++L GNLLAG L P + +I L AG Sbjct: 19 QTPLLAPYLNVLGVSAAMIGFVLGGYAISNLTGNLLAGPFLDRYP--KKWFIAGGLILAG 76 Query: 85 AILLMQAHAQLPWHLLLLRAASGFVLAFXXXXXXXXXXXXXXXXXTQGKYMSGHGIVHTL 144 +L+ Q Q R GF++AF QG+ M G+V T Sbjct: 77 VMLIGQGIVQNTAGFFTFRLMLGFLMAFVSPACSALLGETGKTALEQGEIMGKKGLVLTA 136 Query: 145 ASVVSPAAGAFIVAKAGYSGTFTTLGWLLILTGVMALFSVPKQVQSMSAAKPALPLHSPA 204 A +VSPA G+F+ + GY +F LG+++++ GV A +P++V+ +++ P Sbjct: 137 AGIVSPAVGSFLAVQFGYGQSFVILGYMMMIAGVFAWMMLPERVEGSTSSTPRSKGEGKL 196 Query: 205 QAADRENPRVSRRYYLLPFFVSCSQGVLFFELPLSQGSEGM--ISTGIXXXXXXXXXXXX 262 R+ +L F + +QG + +E+PL +GM TGI Sbjct: 197 SLTAILENRMLYPAFLGGFAIIYAQGTIIYEVPLLIQKQGMSPAVTGILFSLKGLGALAI 256 Query: 263 XXXXXXNRFAPGIRIXXXXXXXXXXXXXXXXXRHIPAGVVLFLLGAAKGVLFPAMASLFI 322 +R R IP V++F GA G+LFPA A++ Sbjct: 257 LCQFWLHRVPVETRTFAGLGVLSLLLYALAIGLAIPIHVMMFFFGACFGMLFPAFATILT 316 Query: 323 SLGGPGRMGRTFSLQSIAMSLGAFAGPVAAGQLRDFVSPYFIAF 366 + G FS+ S +S+GA PV AG L ++ YFIAF Sbjct: 317 VHTPREQYGSVFSIYSAILSIGAVLSPVLAGLLGNWHHSYFIAF 360 >C3HDV3.1 C3HDV3_BACTU Multidrug efflux transporter Length = 390 Score = 70.9 bits (172), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 61/215 (28%), Positives = 98/215 (45%), Gaps = 6/215 (2%) Query: 26 QYPILTPFAISLGAGPAFIGWMMGMYSLTHLPGNLLAGVLXXXPTAPR-RYIVFALTAAG 84 Q PI++ FA SLG P IG ++GMYS ++ GN++AG A + YI LT+ Sbjct: 19 QLPIMSTFAQSLGGSPLIIGLVVGMYSFANMIGNIIAGAAVDKFGAKKILYISMGLTS-- 76 Query: 85 AILLMQAHAQLPWHLLLLRAASGFVLAFXXXXXXXXXXXXXXXXXTQGKYMSGHGIVHTL 144 I+L+ Q LL++R GF F QGK M+ G Sbjct: 77 FIVLLYTVVQSGEQLLIVRFMHGFSDGF-LIPAAFTFLSKQTNAARQGKAMALSGAAVGT 135 Query: 145 ASVVSPAAGAFIVAKAGYSGTFTTLGWLLILTGVMALFSVPKQVQSMSAAKPALPLHSPA 204 A++V PA + A AG F T+ L++L +++LF +P V ++ + Sbjct: 136 AAIVGPAFSGIMKATAGIEWVFITISILMVLGTIVSLFFLPNNVSRKDTSRTQMMNKEDM 195 Query: 205 QAADRENPRVSRRYYLLPFFVSCSQGVLFFELPLS 239 + P + + Y+ F + SQG++ + LP+ Sbjct: 196 FELLKSEPLL--QAYIGAFTLMFSQGIVTYMLPVK 228 >Q6HNE1.1 Q6HNE1_BACHK Probable multidrug efflux transporter Length = 390 Score = 70.9 bits (172), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 61/215 (28%), Positives = 98/215 (45%), Gaps = 6/215 (2%) Query: 26 QYPILTPFAISLGAGPAFIGWMMGMYSLTHLPGNLLAGVLXXXPTAPR-RYIVFALTAAG 84 Q PI++ FA SLG P IG ++GMYS ++ GN++AG A + YI LT+ Sbjct: 19 QLPIMSTFAQSLGGSPLIIGLVVGMYSFANMIGNIIAGAAVDKFGAKKILYISMGLTS-- 76 Query: 85 AILLMQAHAQLPWHLLLLRAASGFVLAFXXXXXXXXXXXXXXXXXTQGKYMSGHGIVHTL 144 I+L+ Q LL++R GF F QGK M+ G Sbjct: 77 FIVLLYTVVQSGEQLLVVRFMHGFSDGF-LIPAAFTFLSKQTNAARQGKAMALSGAAVGT 135 Query: 145 ASVVSPAAGAFIVAKAGYSGTFTTLGWLLILTGVMALFSVPKQVQSMSAAKPALPLHSPA 204 A++V PA + A AG F T+ L++L +++LF +P V ++ + Sbjct: 136 AAIVGPAFSGIMKATAGIEWVFITISILMVLGTIISLFFLPNNVSRKDTSRTQMMNKEDM 195 Query: 205 QAADRENPRVSRRYYLLPFFVSCSQGVLFFELPLS 239 + P + + Y+ F + SQG++ + LP+ Sbjct: 196 FELLKSEPLL--QAYIGAFTLMFSQGIVTYMLPVK 228 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,890,764,725 Number of extensions: 68383063 Number of successful extensions: 260482 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 260478 Number of HSP's successfully gapped: 82 Length of query: 395 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 257 Effective length of database: 1,760,596,133 Effective search space: 452473206181 Effective search space used: 452473206181 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)