BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0105.0011.p (508 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DBA1.1 B1DBA1_9BACL Drug resistance transporter, EmrB/QacA sub... 669 0.0 B3KDK4.1 B3KDK4_9BACI Drug resistance transporter, EmrB/QacA sub... 480 e-133 B1D8X7.1 B1D8X7_9BACL Drug resistance transporter, EmrB/QacA sub... 457 e-126 B1DHG1.1 B1DHG1_9BACL Drug resistance transporter, EmrB/QacA sub... 452 e-125 B3K8J9.1 B3K8J9_9BACI Drug resistance transporter, EmrB/QacA sub... 451 e-125 >B1DBA1.1 B1DBA1_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 495 Score = 669 bits (1725), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/477 (68%), Positives = 379/477 (79%), Gaps = 2/477 (0%) Query: 19 SSSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLI 78 SSVKKGPILF+MILGAF+ATLNQT+MSVA PELM DF+I+A T QWLTTGYMLVNG+LI Sbjct: 8 DSSVKKGPILFVMILGAFIATLNQTIMSVAGPELMKDFSISATTVQWLTTGYMLVNGILI 67 Query: 79 PITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNV 138 PITAFLMQRFTTRELFQ+SM +FLAGT+VSALA+ F +LL GR+IQAAGAGIIMPLL V Sbjct: 68 PITAFLMQRFTTRELFQTSMILFLAGTIVSALASDFNVLLIGRLIQAAGAGIIMPLLMTV 127 Query: 139 ILTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGF 198 ILTLFP EKRG AMGMVG AIIFAPAIGPTLAG++LE++ WE MFYGM+PL IVI+CGF Sbjct: 128 ILTLFPPEKRGGAMGMVGFAIIFAPAIGPTLAGWVLENFKWEMMFYGMIPLALIVILCGF 187 Query: 199 IYLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLF 258 I+L NV+ER+YPKID+ SVILSTVGFG +LYGFSRA SAGW SAEV+ T+ G ++L LF Sbjct: 188 IFLTNVSERTYPKIDVISVILSTVGFGAMLYGFSRAGSAGWESAEVLITIIGGFIALVLF 247 Query: 259 TWRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXX 318 WRQL S +PLLDLR FKYNM+SLTT INIA+T+IMYADMMLLPLYLQNARGYTAMES Sbjct: 248 VWRQLISENPLLDLRAFKYNMYSLTTFINIAITMIMYADMMLLPLYLQNARGYTAMESGL 307 Query: 319 XXXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMST 378 VTG+LFD+FGAKW F+NLTD+T+YTYLVLMST Sbjct: 308 LLLPGALIMGFLMPVTGRLFDKFGAKWLSVAGMIIVIVTTLGFINLTDTTSYTYLVLMST 367 Query: 379 GRRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTTTHL 438 GRRIGMALL+MP+QT GLNQLP++LNAHGTAISNTIRQVAGAVGTSLLVT++T++T +HL Sbjct: 368 GRRIGMALLMMPVQTAGLNQLPNRLNAHGTAISNTIRQVAGAVGTSLLVTILTTQTKSHL 427 Query: 439 XXXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQASDQE 495 ++ MI A+I G+NDAY FFIKRV QASD++ Sbjct: 428 --QDAMASGAAGDQQQMITNATISGMNDAYLVIVGIGVVGLILTFFIKRVAQASDEK 482 >B3KDK4.1 B3KDK4_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 501 Score = 480 bits (1235), Expect = e-133, Method: Compositional matrix adjust. Identities = 236/490 (48%), Positives = 336/490 (68%), Gaps = 4/490 (0%) Query: 22 VKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIPIT 81 VKKGPI+ +++GAF+A LNQT+M+VA P +M + +I AQWLTTG+MLVNGVLIP+T Sbjct: 13 VKKGPIIAALMIGAFVAILNQTLMNVALPSIMENLSIKPTVAQWLTTGFMLVNGVLIPVT 72 Query: 82 AFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNVILT 141 A+L+ RF+TR+LF S+M +F GT++ A++ +F LLTGR++QAAGAGI+MPL+T V L Sbjct: 73 AYLIARFSTRQLFISAMTLFTIGTLLCAISPNFAFLLTGRLVQAAGAGILMPLMTVVFLN 132 Query: 142 LFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGFIYL 201 +FP E RG AMGM+G+A+IFAPAIGPTL+GYI+EHY W +FY +LP + I + G +L Sbjct: 133 IFPIENRGKAMGMMGIAMIFAPAIGPTLSGYIVEHYDWRVLFYMILPFSVIATLIGVFFL 192 Query: 202 KNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLFTWR 261 KNV E S PK+D+ ++ILST+GFG LLYGFS A + GWSS V+ TLA G ++L LF WR Sbjct: 193 KNVTEVSRPKLDMIAIILSTLGFGGLLYGFSDAGNDGWSSTPVVTTLAVGTVALILFVWR 252 Query: 262 QLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXXXXX 321 ++ + +P+L+ R+FKYNMF+LTT+IN+ VT+ MYA M+LLP+YLQ RG+T +ES Sbjct: 253 EMKADNPMLEFRIFKYNMFTLTTLINVIVTMAMYAGMILLPIYLQQIRGFTPVESGLLML 312 Query: 322 XXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMSTGRR 381 +TG +FD+ GA+W F NLT++ +Y ++++ T R Sbjct: 313 PGAILMGIMSPITGAIFDKVGARWLAVIGLAITVITTWEFSNLTETMSYGQIMIIYTARM 372 Query: 382 IGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTTTHLXXX 441 GM++L+MPIQT GLNQLP LNAHGTA+SNT+R V+G++GT++LVTVM+++ + Sbjct: 373 FGMSMLMMPIQTAGLNQLPRSLNAHGTAMSNTLRMVSGSIGTAILVTVMSTQAESRGEAL 432 Query: 442 XXXXXXXXXSKEHMIM---EASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQASDQEPGA 498 ++ ++ EA+I GIN A+ AFFIK+ + +++ A Sbjct: 433 MQSGLFDPKNQADLLKLGNEATIYGINYAFVIATWISVVSLILAFFIKKT-KPAEEPKAA 491 Query: 499 QLKQAIAEKA 508 ++A A A Sbjct: 492 GSREAAATNA 501 >B1D8X7.1 B1D8X7_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 505 Score = 457 bits (1175), Expect = e-126, Method: Compositional matrix adjust. Identities = 226/480 (47%), Positives = 326/480 (67%), Gaps = 5/480 (1%) Query: 20 SSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIP 79 +++K+GPI+ +++ AF+A L+QT+++VA P +M D ++A T QWL+TGY+LVNGVLIP Sbjct: 6 AALKRGPIVASVLIAAFVALLSQTLLNVALPSIMTDLKVSANTVQWLSTGYLLVNGVLIP 65 Query: 80 ITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNVI 139 I+A+L+ RF+TR LF + +F GT+VSALA SF LLTGR+IQAAGAGI+MPL++ + Sbjct: 66 ISAYLISRFSTRSLFVVATSLFTVGTIVSALAPSFETLLTGRIIQAAGAGILMPLMSIIF 125 Query: 140 LTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGFI 199 LT+FP E+RG AMGM+G+A+IFAPA+GPTL+G+++EHY W +FY +LPL+ +I G I Sbjct: 126 LTIFPIEQRGKAMGMMGIAMIFAPAVGPTLSGWVVEHYDWRVLFYIVLPLSLFGLIYGAI 185 Query: 200 YLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLFT 259 +KNV + + K+D+ +VILST+GFG LLYGFS A + GW++ V +L GV+SL F Sbjct: 186 SMKNVTQTAKSKLDIFAVILSTLGFGGLLYGFSDAGTDGWNNVTVYTSLIVGVISLIGFV 245 Query: 260 WRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXXX 319 W QL P+L++RVFK+ M+SLTT+IN+ +T+ M++ M+LLP+YLQ+ G+T ++S Sbjct: 246 WYQLVVKKPILEMRVFKFGMYSLTTVINVIITMAMFSGMILLPIYLQSIMGFTPLKSGLL 305 Query: 320 XXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMSTG 379 +TG +FD+ GA+W F LTD T Y YL+ M T Sbjct: 306 LLPGAILMGIMSPITGMIFDKVGARWLAVTGLVITVYSSYEFSQLTDHTTYGYLITMYTL 365 Query: 380 RRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRT---TT 436 R GM++L+MPIQT G+NQLP +LNAHG+A+S T+R VAGA+GT+ LVT+MT++ T Sbjct: 366 RMFGMSMLMMPIQTAGMNQLPQRLNAHGSAMSQTLRNVAGALGTAFLVTLMTNKAASETK 425 Query: 437 HLXXXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQASDQEP 496 H+ + H+I EA+I GIN ++ AFFI++V +EP Sbjct: 426 HMVATQGIDPNDKAAMAHVIQEATITGINHSFVVATWFAVAALVLAFFIRKV--KPHEEP 483 >B1DHG1.1 B1DHG1_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 592 Score = 452 bits (1162), Expect = e-125, Method: Compositional matrix adjust. Identities = 220/482 (45%), Positives = 323/482 (67%), Gaps = 3/482 (0%) Query: 15 TEAISSSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVN 74 T +S+ ++KGPI+ +++ AF+A L+QTV++VA P++M D ++ +T QWL+ GYMLVN Sbjct: 2 TTVVSNQLRKGPIMASLLIAAFVALLSQTVLNVALPKMMADLDVGESTIQWLSNGYMLVN 61 Query: 75 GVLIPITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPL 134 GVL+PI+A+L+ RFTTR+LF + +F GTVV AL+ F LL GR++QA GAGI+MPL Sbjct: 62 GVLVPISAYLINRFTTRKLFLVASSLFAIGTVVCALSGDFSSLLAGRLVQACGAGILMPL 121 Query: 135 LTNVILTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVI 194 +T V LT+FP E+RG AMG++G+A+IFAPA+GPTL+G+++EHY W +FY +LPL + + Sbjct: 122 MTIVTLTIFPVEERGKAMGLMGVAMIFAPAVGPTLSGWVVEHYDWHVLFYIVLPLAILAV 181 Query: 195 ICGFIYLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLS 254 + G +K+V + S P +D+ SV+LST+GFG LLYGFS A + GW S EVI LA G +S Sbjct: 182 VFGAFSMKDVIKTSRPNLDILSVVLSTLGFGGLLYGFSEAGTEGWDSTEVIVCLAVGAIS 241 Query: 255 LGLFTWRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAM 314 L LF R + +PLL++RVFKY+MFSLT +IN +T+ M++ M+LLP++LQN RG+T + Sbjct: 242 LILFVVRSILMKAPLLEMRVFKYSMFSLTALINAIITMAMFSGMILLPIFLQNIRGFTPL 301 Query: 315 ESXXXXXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLV 374 ES +TG +FD+ GA+W F +L ++Y +++ Sbjct: 302 ESGLLLLPGAILMGIMSPITGMVFDKIGARWLAVIGLIITTVTTYEFSHLEIDSSYNHMM 361 Query: 375 LMSTGRRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRT 434 L T R GM++L+MPIQT GLNQLP +LNAHG+A+S T+R V+GA+GT++LVT+MT++ Sbjct: 362 LFYTLRMFGMSMLMMPIQTAGLNQLPRRLNAHGSAMSQTLRNVSGALGTAILVTIMTNKA 421 Query: 435 TTHLXXXXXXXXXXXXSKEHM---IMEASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQA 491 +H K M ++++I GIN ++ AFFI++V A Sbjct: 422 ASHAKELIIAGNISPSDKAKMGEITIQSTIYGINQSFVVATWLTVAALVLAFFIRKVKPA 481 Query: 492 SD 493 D Sbjct: 482 ED 483 >B3K8J9.1 B3K8J9_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 483 Score = 451 bits (1160), Expect = e-125, Method: Compositional matrix adjust. Identities = 222/449 (49%), Positives = 316/449 (70%), Gaps = 2/449 (0%) Query: 20 SSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIP 79 ++ KG +L I+ILG FL+TLNQT+++VA LM F++TAAT QW++TG+MLVNG+LIP Sbjct: 6 KNINKGILLTILILGCFLSTLNQTLLNVALSNLMDVFDVTAATVQWISTGFMLVNGILIP 65 Query: 80 ITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNVI 139 ITA+LM+RFTTR+LF S+M L G+V+ A A SF +LL GRM+QAAGAGI+MPL+ +V+ Sbjct: 66 ITAYLMKRFTTRQLFISAMSFLLIGSVICAAAPSFVVLLLGRMVQAAGAGIVMPLMMSVV 125 Query: 140 LTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGFI 199 L +FP EKRG+AMG++GLA+IFAPAIGPTLAG+I+E++SW +F G++PL +VI F Sbjct: 126 LAIFPVEKRGSAMGLLGLAMIFAPAIGPTLAGFIVEYHSWRWLFIGLIPLVIMVIALAFK 185 Query: 200 YLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLFT 259 YL NV+E + K+D+ SV+LST+GFG +LYGFS A S GW A VI +L G++ +F Sbjct: 186 YLVNVSETAKSKLDVGSVLLSTIGFGFILYGFSSAGSKGWDDAMVILSLGIGIVVTAIFC 245 Query: 260 WRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXXX 319 RQ+ S PLL+L VFK +F+LT++IN+ VT++MYADM+LLP+YLQ+ RG+TA ++ Sbjct: 246 MRQVKSDDPLLNLSVFKNKIFTLTSLINVLVTMLMYADMILLPIYLQDGRGFTAFDAGLL 305 Query: 320 XXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMSTG 379 VTG+L+DRFGA+ + +L++ST Y YL++ + G Sbjct: 306 LLPGAVVNALMSPVTGRLYDRFGARPLFIIGLLFIIPSMWAVTDLSESTTYMYLMIRTIG 365 Query: 380 RRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTTTHLX 439 RIG++ + MP+ T GLN LP L HGTA++NT+RQ+AGA+GT++++T+ T + T+H Sbjct: 366 LRIGLSFITMPLNTAGLNALPKHLGTHGTAVNNTVRQIAGAIGTAVVITIYTVQATSH-- 423 Query: 440 XXXXXXXXXXXSKEHMIMEASIQGINDAY 468 + E + SI G +DAY Sbjct: 424 AADVMQNNPTTTAELLKSLTSILGASDAY 452 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,282,454,567 Number of extensions: 80194587 Number of successful extensions: 415088 Number of sequences better than 1.0e-05: 10711 Number of HSP's gapped: 405070 Number of HSP's successfully gapped: 10713 Length of query: 508 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 368 Effective length of database: 1,741,754,103 Effective search space: 640965509904 Effective search space used: 640965509904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)