BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0101.0018.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4C4.1 B3K4C4_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 387 e-106 B7DMS6.1 B7DMS6_9BACL 2,5-didehydrogluconate reductase EC=1.1.1.274 371 e-101 C1PQD6.1 C1PQD6_ALIAC Aldo/keto reductase, diketogulonate reduct... 369 e-100 B7GJD4.1 B7GJD4_ANOFW Aldo/keto reductase 367 e-100 C2ZIS4.1 C2ZIS4_BACCE YtbE (Aldo/keto reductase YtbE) 364 4e-99 >B3K4C4.1 B3K4C4_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 276 Score = 387 bits (993), Expect = e-106, Method: Compositional matrix adjust. Identities = 183/259 (70%), Positives = 214/259 (82%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 MPWLGLGVW+ KEG+EVI +VK+A+ GYRSIDTAA Y NEEGVGQAIRESGV R+ELFI Sbjct: 18 MPWLGLGVWKTKEGEEVIQSVKSAIAAGYRSIDTAAIYGNEEGVGQAIRESGVSRDELFI 77 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 TTK+WN DQGYE L+AFE SRKKLGLD++DLYL+HWP KY E+WKAL+HLQKEGLV+ Sbjct: 78 TTKVWNDDQGYEKTLQAFETSRKKLGLDIVDLYLVHWPGKDKYLETWKALIHLQKEGLVR 137 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 SIGVSNFQ HLQ II+DTGVVP V+QVE HPLL+Q+ELL YA+ + I +EAWSPLMQGN Sbjct: 138 SIGVSNFQIRHLQHIIEDTGVVPVVNQVELHPLLSQKELLGYARENHIVLEAWSPLMQGN 197 Query: 181 LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVKV 240 LD P L ++A KYGKT AQ++LRWD+Q GVI IPKS+ RI ENAG FDF LS ED+ Sbjct: 198 LDQPALAQIAEKYGKTTAQVILRWDIQNGVIVIPKSIKDHRIRENAGIFDFELSAEDMAA 257 Query: 241 LEDLNQNHRFGPDPDNFNF 259 ++ LNQN RFG +PD F F Sbjct: 258 IDGLNQNKRFGSNPDEFLF 276 >B7DMS6.1 B7DMS6_9BACL 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 274 Score = 371 bits (953), Expect = e-101, Method: Compositional matrix adjust. Identities = 169/257 (65%), Positives = 209/257 (81%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 MPWLGLGV++A+ G+EV A++ A E GYR IDTAA Y NEE VG+A+RESG+PR E+F+ Sbjct: 18 MPWLGLGVYKAQAGEEVEQAIRFAFEAGYRHIDTAAFYANEESVGKAVRESGLPRSEVFV 77 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 TTK+WN DQGY+ L+AFE S K+LG D +DLYL+HWPV+GKYRE+W+AL + E L + Sbjct: 78 TTKVWNTDQGYDQTLRAFEASLKRLGFDYVDLYLVHWPVSGKYRETWRALERIYDEKLAR 137 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 +IGVSNFQ HHL+DI+ VVPAV+QVE+HPLLTQ L ++ + H IQ+EAWSP+M+GN Sbjct: 138 AIGVSNFQIHHLEDILAHGNVVPAVNQVEYHPLLTQEPLRQFCRKHHIQLEAWSPIMRGN 197 Query: 181 LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVKV 240 LDLPVL+ELA KY KTPAQIVLRWDLQ V+TIPKSV ERI ENA FDF+LSDED++ Sbjct: 198 LDLPVLQELAQKYNKTPAQIVLRWDLQNEVVTIPKSVRRERIFENADLFDFSLSDEDMRR 257 Query: 241 LEDLNQNHRFGPDPDNF 257 + LN+NHRFGPDPD F Sbjct: 258 ISALNRNHRFGPDPDTF 274 >C1PQD6.1 C1PQD6_ALIAC Aldo/keto reductase, diketogulonate reductase EC=1.1.1.274 Length = 274 Score = 369 bits (947), Expect = e-100, Method: Compositional matrix adjust. Identities = 169/257 (65%), Positives = 209/257 (81%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 MPWLGLGV++A+ GDEV A++ A E GYR IDTAA Y NEE VG+A+RESG+PR E+F+ Sbjct: 18 MPWLGLGVYKAQAGDEVEQAIRFAFEAGYRHIDTAAFYANEESVGKAVRESGLPRGEVFV 77 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 TTK+WN DQGY+ L+AFE S K+LG + +DLYL+HWPV+GKYRE+W+AL + E L + Sbjct: 78 TTKVWNTDQGYDQTLRAFEASLKRLGFEYVDLYLVHWPVSGKYRETWRALERIYDEKLAR 137 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 +IGVSNFQ HHL+DI+ VVPAV+QVE+HPLLTQ L ++ + H IQ+EAWSP+M+GN Sbjct: 138 AIGVSNFQIHHLEDILAHGNVVPAVNQVEYHPLLTQEPLRQFCREHHIQLEAWSPIMRGN 197 Query: 181 LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVKV 240 LDLPVL+ELA KY KTPAQIVLRWDLQ V+TIPKSV ERI ENA FDF+LSDED++ Sbjct: 198 LDLPVLQELAQKYHKTPAQIVLRWDLQNEVVTIPKSVRRERIFENADLFDFSLSDEDMRR 257 Query: 241 LEDLNQNHRFGPDPDNF 257 + LN+NHRFGPDPD F Sbjct: 258 INALNRNHRFGPDPDTF 274 >B7GJD4.1 B7GJD4_ANOFW Aldo/keto reductase Length = 268 Score = 367 bits (943), Expect = e-100, Method: Compositional matrix adjust. Identities = 169/261 (64%), Positives = 212/261 (81%), Gaps = 2/261 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 MPWLGLGV++ +EGDEVI +V+TALE GYR IDTAA Y NE+GVGQA+RESG+PR+E+F+ Sbjct: 8 MPWLGLGVYKVQEGDEVIRSVRTALEVGYRHIDTAAFYDNEKGVGQAVRESGIPRDEIFV 67 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 TTK+WN DQGYES L+AF+ S KKLG D +DLYL+HWPV GKY+E++KAL L K+G V+ Sbjct: 68 TTKVWNSDQGYESTLQAFDASLKKLGFDYIDLYLVHWPVKGKYKETYKALEKLYKDGFVR 127 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 +IGVSNFQ HHL+DI+ D + P V+QVEFHP LTQ+ LL + K HDIQ+EAWSPLM+G Sbjct: 128 AIGVSNFQIHHLEDILADCEIKPMVNQVEFHPRLTQKPLLSFCKQHDIQLEAWSPLMRGG 187 Query: 181 --LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDV 238 L+ P L +A KYGKT AQ++LRWDLQ V+TIPKSV ERI +NA FDFTL+ E++ Sbjct: 188 DLLNEPTLVNIAQKYGKTSAQVILRWDLQHEVVTIPKSVTPERIAQNANIFDFTLTKEEM 247 Query: 239 KVLEDLNQNHRFGPDPDNFNF 259 ++ LN+NHR GPDPDNF+F Sbjct: 248 DAIDALNENHRIGPDPDNFDF 268 >C2ZIS4.1 C2ZIS4_BACCE YtbE (Aldo/keto reductase YtbE) Length = 277 Score = 364 bits (934), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 169/260 (65%), Positives = 207/260 (79%), Gaps = 1/260 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 MPW GLGV++ ++G EVI +VK A++ GYRSIDTAA YKNEEGVGQAIRESG+PREELFI Sbjct: 18 MPWFGLGVFKVEDGSEVIDSVKAAIKNGYRSIDTAAIYKNEEGVGQAIRESGIPREELFI 77 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 T+K+WN DQGYES L+AFE + +KLGL+ LDLYL+HWPV GKY ESWKAL L K+G V+ Sbjct: 78 TSKVWNSDQGYESTLQAFETTLEKLGLEYLDLYLVHWPVKGKYTESWKALEQLYKDGRVR 137 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 +IGVSNF HHLQD+ + + P V+QVE+HP L Q EL + K H IQ+EAWSPLMQG Sbjct: 138 AIGVSNFHIHHLQDVFEIAEIKPMVNQVEYHPRLAQEELHTFCKEHGIQLEAWSPLMQGQ 197 Query: 181 -LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVK 239 LD P L+E+A KY K+ AQ++LRWDLQ V+TIPKS+ RI+ENA FDF LS +D+K Sbjct: 198 LLDHPTLQEIATKYNKSTAQVILRWDLQNEVVTIPKSIKEHRIIENANIFDFELSSDDMK 257 Query: 240 VLEDLNQNHRFGPDPDNFNF 259 ++ LN+NHR GPDPDNFNF Sbjct: 258 AIQALNENHRVGPDPDNFNF 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,714,676,808 Number of extensions: 73299881 Number of successful extensions: 222132 Number of sequences better than 1.0e-05: 5343 Number of HSP's gapped: 212236 Number of HSP's successfully gapped: 5399 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)