BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0021.p (217 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KY18.1 Q5KY18_GEOKA Hypothetical conserved protein 134 5e-30 Q2RGR1.1 Q2RGR1_MOOTA Putative uncharacterized protein 129 1e-28 A5D244.1 A5D244_PELTS Predicted integral membrane protein 127 9e-28 Q72YN4.1 Q72YN4_BACC1 Membrane protein, putative 125 4e-27 B7HTU4.1 B7HTU4_BACC7 Conserved membrane protein YvaZ 124 5e-27 >Q5KY18.1 Q5KY18_GEOKA Hypothetical conserved protein Length = 214 Score = 134 bits (338), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 66/155 (42%), Positives = 97/155 (62%) Query: 14 VILGVLSLGYALANYGKLPDQLPAHFDIRGEADRYWSKGSVIAFTGFLGLIFPLAMQFIK 73 ++L VL+ +LA LPDQ+ H++ GEAD + SK L +F M + Sbjct: 8 IVLTVLAYFLSLAALPYLPDQVAIHWNASGEADGFSSKWFGALLLPVLMTVFTFLMAVLP 67 Query: 74 SVDPKKENYSKFQNAYKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFIVV 133 +DPK+ENY++FQ +Y+M+ A++ F A+ +T++Y L +I G + + IG LF+V+ Sbjct: 68 KLDPKRENYARFQTSYRMVNAALSCFFLALHAVTLAYNLGFSIDVGAVMPLGIGGLFLVI 127 Query: 134 GNFMPQIRDNYFTGIRTAWTLASPEVWRKTHRFSG 168 GN+MP+I+ NYF GIRT WTLAS VW KTHR G Sbjct: 128 GNYMPKIKHNYFIGIRTPWTLASETVWHKTHRLGG 162 >Q2RGR1.1 Q2RGR1_MOOTA Putative uncharacterized protein Length = 223 Score = 129 bits (325), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 68/164 (41%), Positives = 100/164 (60%), Gaps = 11/164 (6%) Query: 28 YGKLPDQLPAHFDIRGEAD----RYWSKGSVIAFTGFLGLIFPLAMQFIKSVDPKKENYS 83 Y LPD +P+H++ RGE D R+W ++ TG + L+ + F+ +DPK+ENY Sbjct: 33 YPHLPDLVPSHWNFRGEVDNYFNRFWGAFALPLMTGGIYLL----LLFVPYLDPKRENYP 88 Query: 84 KFQNAYKMIRLAIAALFDAM--LVLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIR 141 +F AY+++RL + + L V+ G N+ G++ +AIGLLFI++GN++PQ R Sbjct: 89 RFDRAYQVVRLGMVFFMGGIYATTLVVALGGPANL-VGRVVPLAIGLLFILIGNYLPQAR 147 Query: 142 DNYFTGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLP 185 NYF GIRT WTLA+ EVWR+THRFSG ++ FLP Sbjct: 148 LNYFFGIRTPWTLANEEVWRRTHRFSGYTFILAGFMFIVAAFLP 191 >A5D244.1 A5D244_PELTS Predicted integral membrane protein Length = 219 Score = 127 bits (318), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 69/208 (33%), Positives = 105/208 (50%), Gaps = 12/208 (5%) Query: 12 VIVILGVLSLGYALANYGKLPDQLPAHFDIRGEADRYWSKGSVIAFTGFLGLIFPLA--- 68 +++I+G L+ G AL Y +LP+++P+H++ RGE D Y S+ F G G+ A Sbjct: 12 LLLIIGSLAAGAAL--YPQLPERVPSHWNFRGEVDGYSSR-----FWGAFGIPLMTAGIY 64 Query: 69 --MQFIKSVDPKKENYSKFQNAYKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAI 126 M + +DP+++NY KF Y++ R + + + + L I +I M I Sbjct: 65 VMMLVLPLIDPRRQNYQKFAGVYRLFRAVMVIFMTGLYAVVLLSALGHQIAVDRITMAGI 124 Query: 127 GLLFIVVGNFMPQIRDNYFTGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLPQ 186 LLF+V+GNFM QIR NYF GI+T WTLA +VW+KTHR G +W+ + Sbjct: 125 SLLFVVLGNFMGQIRHNYFVGIKTPWTLADEQVWQKTHRLGGRLWVAAGLVGLAASLIGG 184 Query: 187 XXXXXXXXXXXXXXXXXPFAYSWLISSR 214 P YS+L+ R Sbjct: 185 AAGGIVLAVSLGTATIIPVVYSYLVFRR 212 >Q72YN4.1 Q72YN4_BACC1 Membrane protein, putative Length = 212 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 59/157 (37%), Positives = 94/157 (59%), Gaps = 2/157 (1%) Query: 30 KLPDQLPAHFDIRGEADRYWSK-GSVIAFTGFLGLIFPLAMQFIKSVDPKKENYSKFQNA 88 LP+++P+H+++ GE D + SK G +I + I+ L + + +DPK +NY KF Sbjct: 24 NLPEEVPSHWNVSGEVDGHMSKMGGMIFDVAIMVFIYAL-LTVLPKIDPKYKNYDKFSKG 82 Query: 89 YKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIRDNYFTGI 148 Y +I ++ L + ++ + GL +IP + +GLLFIV+GN++PQ + NYF GI Sbjct: 83 YNVINYSVLILLFLVNIIGIGAGLGYDIPMNSTPHILVGLLFIVIGNYLPQCKPNYFVGI 142 Query: 149 RTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLP 185 +T WTL++ EVWRKTHRFSG +++ F P Sbjct: 143 KTPWTLSNEEVWRKTHRFSGKVFVVLGVIMILSIFAP 179 >B7HTU4.1 B7HTU4_BACC7 Conserved membrane protein YvaZ Length = 212 Score = 124 bits (311), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 59/157 (37%), Positives = 93/157 (59%), Gaps = 2/157 (1%) Query: 30 KLPDQLPAHFDIRGEADRYWSK-GSVIAFTGFLGLIFPLAMQFIKSVDPKKENYSKFQNA 88 LP+++P+H+++ GE D + SK G +I + I+ L + + +DPK +NY KF Sbjct: 24 NLPEEVPSHWNVSGEVDGHMSKMGGMIFDVAIMVFIYAL-LTVLPKIDPKYKNYDKFSKG 82 Query: 89 YKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIRDNYFTGI 148 Y +I ++ L + ++ + GL IP + +GLLFIV+GN++PQ + NYF GI Sbjct: 83 YNVINYSVLILLFLVNIIGIGAGLGYEIPMNSTPHILVGLLFIVIGNYLPQCKPNYFVGI 142 Query: 149 RTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLP 185 +T WTL++ EVWRKTHRFSG +++ F P Sbjct: 143 KTPWTLSNEEVWRKTHRFSGKVFVVLGVIMILSIFAP 179 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.140 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,172,296,452 Number of extensions: 44073159 Number of successful extensions: 134797 Number of sequences better than 1.0e-05: 173 Number of HSP's gapped: 134713 Number of HSP's successfully gapped: 173 Length of query: 217 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 87 Effective length of database: 1,835,964,253 Effective search space: 159728890011 Effective search space used: 159728890011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)