BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0007.p (371 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E396.1 B9E396_CLOK1 Putative uncharacterized protein 504 e-141 A5MZA1.1 A5MZA1_CLOK5 Methionine synthase-related protein 504 e-141 A6LVV9.1 A6LVV9_CLOB8 Methionine synthase, vitamin-B12 independent 497 e-139 B5XQX5.1 B5XQX5_KLEP3 Methionine synthase, vitamin-B12 independe... 423 e-116 A7MLB3.1 A7MLB3_ENTS8 Putative uncharacterized protein 422 e-116 >B9E396.1 B9E396_CLOK1 Putative uncharacterized protein Length = 372 Score = 504 bits (1299), Expect = e-141, Method: Compositional matrix adjust. Identities = 229/371 (61%), Positives = 287/371 (77%) Query: 1 MISPVHGSKRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQ 60 M + V G KR+ PP+RYDIVGSFLRPE ++ AR + + EI+ +L E+E+ EI+KL+++ Sbjct: 1 MNNQVIGRKRDIPPYRYDIVGSFLRPEEIRLARRQYEKKEISKEELKEVEDIEIAKLVKK 60 Query: 61 EKALGLKAVTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFG 120 +K +GL+AVTDGEFRR WWHLDFFLG+ GT++I ++QG ++ + +AE+FR++ I F Sbjct: 61 QKKIGLRAVTDGEFRRGWWHLDFFLGIKGTERIKINQGSNSYNKLMKAETFRVIDNIKFE 120 Query: 121 DHPMVSQFVELQQMAGDTLAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDAI 180 +HPM+ F L+ + + K +IPSPALFHFVESYN N Y D E DII VY+ AI Sbjct: 121 NHPMIDHFKYLKGITEGCIPKFSIPSPALFHFVESYNSNRFYKDNEKFLCDIIDVYKHAI 180 Query: 181 RAFYEAGCRYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMT 240 +AFY+AGCRYLQLDDTTWGTLCS HR+ G+DPD+LA+DYVRLINESI+ P DMT Sbjct: 181 KAFYDAGCRYLQLDDTTWGTLCSEIHRSRYVELGVDPDELAKDYVRLINESISCCPKDMT 240 Query: 241 IALHVCRGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIRDQQVV 300 I LH+CRGNF S WFAAGGYEPV++ LF +VDAFFLEYDNERSGDF+PLRF++DQ VV Sbjct: 241 IVLHICRGNFHSMWFAAGGYEPVSKELFSNTKVDAFFLEYDNERSGDFSPLRFVKDQIVV 300 Query: 301 LGLVTTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLR 360 LGLVTTKHGGLESKE+ V ID+LCLS QCGFASTEEGN++TEEEQW KL Sbjct: 301 LGLVTTKHGGLESKEELKARIFEACKYVDIDKLCLSPQCGFASTEEGNLITEEEQWAKLE 360 Query: 361 LIIETADEVWV 371 LI+ETA E+W+ Sbjct: 361 LIVETAKEIWI 371 >A5MZA1.1 A5MZA1_CLOK5 Methionine synthase-related protein Length = 372 Score = 504 bits (1299), Expect = e-141, Method: Compositional matrix adjust. Identities = 229/371 (61%), Positives = 287/371 (77%) Query: 1 MISPVHGSKRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQ 60 M + V G KR+ PP+RYDIVGSFLRPE ++ AR + + EI+ +L E+E+ EI+KL+++ Sbjct: 1 MNNQVIGRKRDIPPYRYDIVGSFLRPEEIRLARRQYEKKEISKEELKEVEDIEIAKLVKK 60 Query: 61 EKALGLKAVTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFG 120 +K +GL+AVTDGEFRR WWHLDFFLG+ GT++I ++QG ++ + +AE+FR++ I F Sbjct: 61 QKKIGLRAVTDGEFRRGWWHLDFFLGIKGTERIKINQGSNSYNKLMKAETFRVIDNIKFE 120 Query: 121 DHPMVSQFVELQQMAGDTLAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDAI 180 +HPM+ F L+ + + K +IPSPALFHFVESYN N Y D E DII VY+ AI Sbjct: 121 NHPMIDHFKYLKGITEGCIPKFSIPSPALFHFVESYNSNRFYKDNEKFLCDIIDVYKHAI 180 Query: 181 RAFYEAGCRYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMT 240 +AFY+AGCRYLQLDDTTWGTLCS HR+ G+DPD+LA+DYVRLINESI+ P DMT Sbjct: 181 KAFYDAGCRYLQLDDTTWGTLCSEIHRSRYVELGVDPDELAKDYVRLINESISCCPKDMT 240 Query: 241 IALHVCRGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIRDQQVV 300 I LH+CRGNF S WFAAGGYEPV++ LF +VDAFFLEYDNERSGDF+PLRF++DQ VV Sbjct: 241 IVLHICRGNFHSMWFAAGGYEPVSKELFSNTKVDAFFLEYDNERSGDFSPLRFVKDQIVV 300 Query: 301 LGLVTTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLR 360 LGLVTTKHGGLESKE+ V ID+LCLS QCGFASTEEGN++TEEEQW KL Sbjct: 301 LGLVTTKHGGLESKEELKARIFEACKYVDIDKLCLSPQCGFASTEEGNLITEEEQWAKLE 360 Query: 361 LIIETADEVWV 371 LI+ETA E+W+ Sbjct: 361 LIVETAKEIWI 371 >A6LVV9.1 A6LVV9_CLOB8 Methionine synthase, vitamin-B12 independent Length = 373 Score = 497 bits (1279), Expect = e-139, Method: Compositional matrix adjust. Identities = 232/371 (62%), Positives = 285/371 (76%), Gaps = 1/371 (0%) Query: 1 MISPVHGSKRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQ 60 M + + G+KRN+PPFRYDIVGSFLR + +K AR + GEI +L IE+ EI KL+E+ Sbjct: 1 MSNEIIGTKRNSPPFRYDIVGSFLRTDEIKIARKQYNSGEINAEELRSIEDIEIRKLIEK 60 Query: 61 EKALGLKAVTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFG 120 EK +GL AVTDGEFRRSWWHLDFF+G+ GT+KI+L+QG S + + +AESF+IV KI F Sbjct: 61 EKEIGLIAVTDGEFRRSWWHLDFFVGIEGTKKITLNQGGSVQGSRTKAESFQIVDKIRFN 120 Query: 121 DHPMVSQFVELQQMAGD-TLAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDA 179 HPM+ F L + D +AK TIPSP+LFHFV+S+N N VY E L DII VY+ A Sbjct: 121 KHPMLEHFKYLNSIVKDQNIAKFTIPSPSLFHFVQSHNKNNVYGSDEELMEDIISVYKYA 180 Query: 180 IRAFYEAGCRYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADM 239 I+ FY+AGCRYLQ DDT WGTLCS RHR++ RS+GI+P+ LA++YVRLINES+ +P DM Sbjct: 181 IKEFYDAGCRYLQFDDTAWGTLCSERHRSYFRSQGINPEALAKNYVRLINESVKEKPEDM 240 Query: 240 TIALHVCRGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIRDQQV 299 I LHVCRGN S FA+GGYEP+AE LF A+VD FFLEYD+ER GDF PLRFI DQ V Sbjct: 241 IITLHVCRGNLHSIGFASGGYEPIAEELFSNAKVDGFFLEYDSERCGDFKPLRFINDQFV 300 Query: 300 VLGLVTTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKL 359 VLGLVTTKHGGLESKEQ V I++LCLS QCGFASTEEGN++TEEEQW+K+ Sbjct: 301 VLGLVTTKHGGLESKEQLKNRIAEASQYVDINKLCLSPQCGFASTEEGNIITEEEQWEKI 360 Query: 360 RLIIETADEVW 370 +L+IETA+E+W Sbjct: 361 KLVIETANEIW 371 >B5XQX5.1 B5XQX5_KLEP3 Methionine synthase, vitamin-B12 independent family protein Length = 367 Score = 423 bits (1087), Expect = e-116, Method: Compositional matrix adjust. Identities = 203/366 (55%), Positives = 255/366 (69%), Gaps = 4/366 (1%) Query: 9 KRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKA 68 +R PFR DIVGSFLRP+++K AR +G I GQL EIEN I L++Q+ GL Sbjct: 2 QRQQAPFRADIVGSFLRPDSIKQARQQLAEGIIDAGQLREIENNAIRHLVQQQCDCGLHV 61 Query: 69 VTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQF 128 VTDGEFRR+WWH DFF G+ G ++ +QG +A R+ GK+AFGDHPM+ F Sbjct: 62 VTDGEFRRAWWHFDFFDGLQGVERYDAEQGIQFNGVQTKAHGVRVTGKLAFGDHPMLEDF 121 Query: 129 VELQQMAGDTLAKMTIPSPALFHFVESYNGNE--VYPDREALFRDIIQVYQDAIRAFYEA 186 L+ ++GD KMTIPSP++ HF + VYPD F D+ ++DAIRAFY+A Sbjct: 122 RYLKSISGDAQPKMTIPSPSVLHFRGGRKDIDATVYPDLSDYFDDLATTWRDAIRAFYDA 181 Query: 187 GCRYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMTIALHVC 246 GCRYLQLDDT W LCS R +R RG DPD LA+ Y R++N+++ G+PAD+T+ LHVC Sbjct: 182 GCRYLQLDDTVWAYLCSDAQRQQVRERGDDPDALARIYARVLNQALEGKPADLTVGLHVC 241 Query: 247 RGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIR--DQQVVLGLV 304 RGNFRSTW + GGYEPVAEVLFG VDAFFLEYDN+RSGDFAPLRFIR QQVVLGL+ Sbjct: 242 RGNFRSTWISEGGYEPVAEVLFGGVNVDAFFLEYDNDRSGDFAPLRFIRPGHQQVVLGLI 301 Query: 305 TTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLRLIIE 364 TTK+G LE+ + VP++Q+CLS QCGFASTEEGN L+E++QW K+RL+ Sbjct: 302 TTKNGELENPQGVKARLAEAAQYVPLEQICLSPQCGFASTEEGNALSEDQQWQKVRLVTS 361 Query: 365 TADEVW 370 A +VW Sbjct: 362 IAADVW 367 >A7MLB3.1 A7MLB3_ENTS8 Putative uncharacterized protein Length = 400 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 205/367 (55%), Positives = 257/367 (70%), Gaps = 6/367 (1%) Query: 9 KRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKA 68 +R PFR DIVGSFLRP+A+K AR GEI L +IE+ I +EQ+ A GL Sbjct: 35 QRQHAPFRADIVGSFLRPDAIKQARQQFAAGEIDAAHLRKIEDDAIRHAVEQQCACGLHV 94 Query: 69 VTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQF 128 VTDGEFRR+WWHLDFF + G + + ++QG +A+S R+ GK+AFGDHPM+ F Sbjct: 95 VTDGEFRRAWWHLDFFGALQGVELVEVNQGIQFNGIQTKAQSVRVTGKVAFGDHPMLEDF 154 Query: 129 VELQQMAGDTLAKMTIPSPALFHF---VESYNGNEVYPDREALFRDIIQVYQDAIRAFYE 185 L+ ++G+ KMTIPSP++ HF + + N VYPD + F D+ ++DAIRAFY+ Sbjct: 155 RFLKSVSGNAEPKMTIPSPSVLHFRGGAAAIDRN-VYPDLKDYFDDLATTWRDAIRAFYD 213 Query: 186 AGCRYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMTIALHV 245 AGCRYLQLDDT W LCS R +R RG DPD+LA+ Y R++N+++ G+P D+TI LHV Sbjct: 214 AGCRYLQLDDTVWAYLCSDEQRRQIRERGDDPDELARIYARVLNQALEGKPEDLTIGLHV 273 Query: 246 CRGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIR--DQQVVLGL 303 CRGNFRS+W A GGYEPVAEVLFG VDAFFLEYDN+RSGDFAPLRFIR QQVVLGL Sbjct: 274 CRGNFRSSWIAEGGYEPVAEVLFGTVNVDAFFLEYDNDRSGDFAPLRFIRPGKQQVVLGL 333 Query: 304 VTTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLRLII 363 +TTK+G LE+ E V I+Q+CLS QCGFASTEEGN +T EQW+K+RL+ Sbjct: 334 ITTKNGELENPELIKARLEEAAKYVDINQICLSPQCGFASTEEGNSITPAEQWEKVRLVT 393 Query: 364 ETADEVW 370 A EVW Sbjct: 394 SVASEVW 400 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,164,237,963 Number of extensions: 86357160 Number of successful extensions: 178553 Number of sequences better than 1.0e-05: 330 Number of HSP's gapped: 177546 Number of HSP's successfully gapped: 330 Length of query: 371 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 234 Effective length of database: 1,770,017,148 Effective search space: 414184012632 Effective search space used: 414184012632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)