BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0003.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5R7.1 B3K5R7_9BACI Oxidoreductase domain protein 504 e-141 B3KJD3.1 B3KJD3_9BACI Oxidoreductase domain protein 430 e-119 Q8EKW1.1 Q8EKW1_OCEIH NADH-dependent dehydrogenase 414 e-114 Q71XK3.1 Q71XK3_LISMF Oxidoreductase, Gfo/Idh/MocA family 412 e-113 C1KXB7.1 C1KXB7_LISMC Putative oxidoreductase 412 e-113 >B3K5R7.1 B3K5R7_9BACI Oxidoreductase domain protein Length = 360 Score = 504 bits (1298), Expect = e-141, Method: Compositional matrix adjust. Identities = 234/286 (81%), Positives = 259/286 (90%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 VLTPN SHAEISIA+LEAGKHVMC RTGKKLTIGYNNRFRED Sbjct: 75 VLTPNDSHAEISIASLEAGKHVMCEKPMAKTAADAKLMMETAKRTGKKLTIGYNNRFRED 134 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 SLYLKQ+CEAGELG+IYFAKAHA+RRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM Sbjct: 135 SLYLKQVCEAGELGNIYFAKAHAVRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 194 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK+VLGTT+HELSR+ENAANAWGPWDP++F+VEDSAFGMIVMENGATIMLESSWA Sbjct: 195 MDNYKPKIVLGTTYHELSRKENAANAWGPWDPEKFTVEDSAFGMIVMENGATIMLESSWA 254 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN+L+VDEAKCSLSG+E GADMK GLRINGEK RLYTNEIEL++GGVAFY+G++E APD Sbjct: 255 LNTLEVDEAKCSLSGSEGGADMKNGLRINGEKFSRLYTNEIELNSGGVAFYDGKKEQAPD 314 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 EMR+WIEAIDQDK+PVVTPEQAYVVSRILEALYES++ GKA+YL+ Sbjct: 315 VEMRRWIEAIDQDKDPVVTPEQAYVVSRILEALYESAKIGKAVYLD 360 >B3KJD3.1 B3KJD3_9BACI Oxidoreductase domain protein Length = 361 Score = 430 bits (1105), Expect = e-119, Method: Compositional matrix adjust. Identities = 198/286 (69%), Positives = 236/286 (82%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPN SH+EI++A+LEAGKHVMC RTGKKLTIGYNNRFR+D Sbjct: 75 VCTPNDSHSEITVASLEAGKHVMCEKPMAKTVSEAQAMLDAAKRTGKKLTIGYNNRFRDD 134 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S+YLK +CE GELG IY+ KA AIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLW+ Sbjct: 135 SMYLKGICEEGELGEIYYGKALAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWL 194 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK VLG+T H+L +RENAANA+GPWDPK+F+VEDSAFG I MENGATI+LESSWA Sbjct: 195 MDNYKPKSVLGSTFHKLGQRENAANAFGPWDPKEFTVEDSAFGFITMENGATIVLESSWA 254 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN L++ EAK L+GTE GADMK GLRINGEK+ RLY +++L+AGGVAFY G +E+ D Sbjct: 255 LNVLEIGEAKAVLAGTEGGADMKDGLRINGEKMSRLYETKVDLNAGGVAFYSGSKENDSD 314 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 E R W+EAI +DK+PVV PEQA+VV++ILEA+YES++TGKA+Y + Sbjct: 315 REARMWLEAIIEDKDPVVKPEQAFVVTQILEAIYESAKTGKAVYFD 360 >Q8EKW1.1 Q8EKW1_OCEIH NADH-dependent dehydrogenase Length = 360 Score = 414 bits (1063), Expect = e-114, Method: Compositional matrix adjust. Identities = 192/286 (67%), Positives = 228/286 (79%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPN SHAEISIAAL GKHVMC + KKLTIGYNNRFR D Sbjct: 75 VCTPNNSHAEISIAALHGGKHVMCEKPMAKTAEEAKAMIDAAEKCNKKLTIGYNNRFRTD 134 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S YLK++C+ G+LG IYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM Sbjct: 135 SQYLKKVCQRGDLGHIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 194 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK V+GTTHH L ++++AAN WG W+P +F+VED+AFG I M+NGATI+LESSWA Sbjct: 195 MDNYEPKSVMGTTHHLLGKKKDAANPWGTWNPNEFTVEDAAFGFITMKNGATIILESSWA 254 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LNSLDVDEAKCSLSGTE GADMK GLR++GE LG LYT +++L GGVAFY+G ES + Sbjct: 255 LNSLDVDEAKCSLSGTEGGADMKDGLRLHGEDLGELYTKQVDLGPGGVAFYDGNTESDAE 314 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 E R WIE+I +KE V P++A VV++ILEA+Y+S++TGKA+Y + Sbjct: 315 LEARLWIESIINEKEATVQPKEALVVTQILEAIYQSAKTGKAVYFD 360 >Q71XK3.1 Q71XK3_LISMF Oxidoreductase, Gfo/Idh/MocA family Length = 358 Score = 412 bits (1058), Expect = e-113, Method: Compositional matrix adjust. Identities = 192/286 (67%), Positives = 228/286 (79%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPNISHAEISIAA+EAGKHVMC RTGKKLTIGY NRFR+D Sbjct: 73 VCTPNISHAEISIAAMEAGKHVMCEKPMAKTTEEAKSMIEAANRTGKKLTIGYQNRFRKD 132 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S YL Q+CE GELG IY+AKA AIRRRAVPTWGVFLDEE QGGGPLIDIGTHALDLTLWM Sbjct: 133 SDYLHQVCENGELGDIYYAKAKAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWM 192 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK V+G ++H+L+++ENAANAWG WDP +F+VEDSAFG I MENGATI+LE+SWA Sbjct: 193 MDNYKPKYVVGNSYHKLAKKENAANAWGSWDPNKFTVEDSAFGFITMENGATIVLEASWA 252 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN+LDV EA+ SLSGTE GADM+ GLRINGE ++Y +I+L AGGV FY+G + Sbjct: 253 LNTLDVGEARTSLSGTEGGADMEDGLRINGEAHSQMYEKKIQLEAGGVDFYDGTGDDPAL 312 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 E QW+EAI+++ +PVV PEQA VV++ILEA+YESS+TG+ +Y N Sbjct: 313 IEAEQWLEAIEKNTDPVVKPEQALVVTQILEAIYESSKTGQPVYFN 358 >C1KXB7.1 C1KXB7_LISMC Putative oxidoreductase Length = 358 Score = 412 bits (1058), Expect = e-113, Method: Compositional matrix adjust. Identities = 192/286 (67%), Positives = 228/286 (79%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPNISHAEISIAA+EAGKHVMC RTGKKLTIGY NRFR+D Sbjct: 73 VCTPNISHAEISIAAMEAGKHVMCEKPMAKTTEEAKSMIEAANRTGKKLTIGYQNRFRKD 132 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S YL Q+CE GELG IY+AKA AIRRRAVPTWGVFLDEE QGGGPLIDIGTHALDLTLWM Sbjct: 133 SDYLHQVCENGELGDIYYAKAKAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWM 192 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK V+G ++H+L+++ENAANAWG WDP +F+VEDSAFG I MENGATI+LE+SWA Sbjct: 193 MDNYKPKYVVGNSYHKLAKKENAANAWGSWDPNKFTVEDSAFGFITMENGATIVLEASWA 252 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN+LDV EA+ SLSGTE GADM+ GLRINGE ++Y +I+L AGGV FY+G + Sbjct: 253 LNTLDVGEARTSLSGTEGGADMEDGLRINGEAHSQMYEKKIQLEAGGVDFYDGTGDDPAL 312 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 E QW+EAI+++ +PVV PEQA VV++ILEA+YESS+TG+ +Y N Sbjct: 313 IEAEQWLEAIEKNTDPVVKPEQALVVTQILEAIYESSKTGQPVYFN 358 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,654,729,969 Number of extensions: 65698626 Number of successful extensions: 158776 Number of sequences better than 1.0e-05: 617 Number of HSP's gapped: 158968 Number of HSP's successfully gapped: 617 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)