BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0002.p (517 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2QCB3.1 C2QCB3_BACCE Lysyl-tRNA synthetase 635 e-180 C2WMW3.1 C2WMW3_BACCE Lysyl-tRNA synthetase 633 e-179 C2YS39.1 C2YS39_BACCE Lysyl-tRNA synthetase 633 e-179 B7H5N0.1 B7H5N0_BACC4 Lysyl-tRNA synthetase EC=6.1.1.6 630 e-178 C3FKN8.1 C3FKN8_BACTB Lysyl-tRNA synthetase 629 e-178 >C2QCB3.1 C2QCB3_BACCE Lysyl-tRNA synthetase Length = 517 Score = 635 bits (1638), Expect = e-180, Method: Compositional matrix adjust. Identities = 296/518 (57%), Positives = 378/518 (72%), Gaps = 2/518 (0%) Query: 1 MHWSEKIANRLIESHPEQQTFVCASGISPSGHIHIGNFREIVTTYFVSRALEKAGKQVRF 60 MHW+ +IA+ LI HP ++TFVCASGISPSG +HIGNFREI+TTYFV RAL+ GK+ RF Sbjct: 1 MHWAYEIAHELIRKHPNKETFVCASGISPSGSVHIGNFREIITTYFVVRALQDLGKKTRF 60 Query: 61 IFSWDDFDRFRKVPLQVDPSFEQYIGRPYTQVPCPYGCHTSYAEHFEQEFEEALQSFGIR 120 IFSWDD+DRFRKVP +DPSF +YIG PY +P PYGCH SYAEHFE+EFE++LQ+FGI Sbjct: 61 IFSWDDYDRFRKVPKNIDPSFAKYIGMPYCDIPDPYGCHNSYAEHFEKEFEKSLQAFGIE 120 Query: 121 PEFIYQSKEYQSRRYAPGILHALRSRGEIYDILMSFKTGEASEADRAAFYPVSIYCGDCG 180 EFIYQ EY+SRRY IL AL R EIYDILMSFKTGE SE +R +FYPV++YC CG Sbjct: 121 VEFIYQHAEYRSRRYNKNILEALYRRKEIYDILMSFKTGECSEEERESFYPVTLYCEKCG 180 Query: 181 KDSTTVHSFDEDKELLTYSCTCGHHSSLSIPEADNIKLQWKIDWPMRWNLEQVLFEPGGR 240 KD+TT+ FDE + + Y C CG+ + LS+ + +KL WKIDWPMRW +E V+FEPGGR Sbjct: 181 KDATTITHFDEVLKSVQYECVCGNQNELSVLNTNKMKLNWKIDWPMRWMIEDVIFEPGGR 240 Query: 241 DHSSETGSYNVASVISERIFGSAPPMYEPYEFIRIKGSFAKMSSSSGNNYTPDDLLKVYA 300 DHSSETGSYNV+ I+ IF P Y Y+FI IKG KMSSSSGN+ TP +LLKVY Sbjct: 241 DHSSETGSYNVSKEIAREIFNGEAPHYVAYDFIGIKGHHEKMSSSSGNSITPSELLKVYL 300 Query: 301 PENILFLFAKYQPGAAFHIGMDEDVLRNYTEFERYRKAYGSLTL-TDDIRAALELSLIHP 359 PE ILF+FAKY+P AAFHIG+DEDV+RNYTE+ER + +Y + TL +D+ A++L I Sbjct: 301 PEVILFMFAKYRPNAAFHIGLDEDVIRNYTEYERLKDSYENKTLKNEDLFDAIKLYRIDS 360 Query: 360 LDEKAPGFGPIAGLLPLIHFDRKLLLEIMTRNGEEVDVQGLEQTAERAEYWIRHWMPQKL 419 ++ P F +AG LPL++FD +L + + + + + RAEYWIR++ +KL Sbjct: 361 GLKEYPKFNQVAGTLPLLNFDSSILQDTLEKIDRSYTLDAMIAICNRAEYWIRNFQSEKL 420 Query: 420 VTVNTRRNNSLYETLEAHEQEWLAAFCRLLRTGGMEDEPLMKAIYAICYDEDKKSMKNNQ 479 +TVN RN Y TL+ ++ WL C+ LR+ + LM+ +Y+IC+ E+KK MK NQ Sbjct: 421 ITVNQERNIEFYNTLDDRQKGWLVEVCKTLRSNN-DYSNLMEQLYSICHHENKKIMKENQ 479 Query: 480 KRLFTLIYQLVLGQETGPRIPVLIQAVGIEKLLALLDF 517 K+LFT+IYQL++ Q GPRIP+LI VGIEK + LLDF Sbjct: 480 KQLFTIIYQLIMNQSNGPRIPLLIHVVGIEKFITLLDF 517 >C2WMW3.1 C2WMW3_BACCE Lysyl-tRNA synthetase Length = 517 Score = 633 bits (1633), Expect = e-179, Method: Compositional matrix adjust. Identities = 294/518 (56%), Positives = 379/518 (73%), Gaps = 2/518 (0%) Query: 1 MHWSEKIANRLIESHPEQQTFVCASGISPSGHIHIGNFREIVTTYFVSRALEKAGKQVRF 60 MHW+ ++A+ LI HP ++TFVCASGISPSG +HIGNFREIVTTYFV RAL+ GK+ RF Sbjct: 1 MHWAYEVAHELIRKHPNKETFVCASGISPSGSVHIGNFREIVTTYFVVRALQGLGKKTRF 60 Query: 61 IFSWDDFDRFRKVPLQVDPSFEQYIGRPYTQVPCPYGCHTSYAEHFEQEFEEALQSFGIR 120 IFSWDD+DRFRKVP +DPSF +YIG PY +P P GCH SYAEHFE+EFE++LQ+FGI Sbjct: 61 IFSWDDYDRFRKVPKNIDPSFAKYIGMPYCDIPDPSGCHNSYAEHFEKEFEKSLQAFGIE 120 Query: 121 PEFIYQSKEYQSRRYAPGILHALRSRGEIYDILMSFKTGEASEADRAAFYPVSIYCGDCG 180 EFIYQ EY+S RY IL AL R EIYDILM FKTGE+SE +R FYP ++YC CG Sbjct: 121 VEFIYQYDEYRSGRYNKNILEALYKRKEIYDILMDFKTGESSEEERNDFYPATLYCERCG 180 Query: 181 KDSTTVHSFDEDKELLTYSCTCGHHSSLSIPEADNIKLQWKIDWPMRWNLEQVLFEPGGR 240 KD+TT+ FDE + + Y C CG+ LS+ + +KL WKIDWPMRW +E V+FEPGGR Sbjct: 181 KDTTTITHFDEVLKTVRYECECGNEKELSVLNTNKMKLNWKIDWPMRWMIEDVIFEPGGR 240 Query: 241 DHSSETGSYNVASVISERIFGSAPPMYEPYEFIRIKGSFAKMSSSSGNNYTPDDLLKVYA 300 DHSSETGSYNV+ I+ +IF P Y Y+FI IKG+ KMSSSSGN+ TP DLLKVY Sbjct: 241 DHSSETGSYNVSKEIARKIFNREAPHYVAYDFIGIKGNHEKMSSSSGNSITPSDLLKVYI 300 Query: 301 PENILFLFAKYQPGAAFHIGMDEDVLRNYTEFERYRKAYGSLTL-TDDIRAALELSLIHP 359 PE ILF+FAKY+PGAAFHIG+DEDV+RNYTE+ER + +Y + TL +D+ A++LS + Sbjct: 301 PEVILFMFAKYKPGAAFHIGLDEDVIRNYTEYERLKDSYENKTLKNEDLFDAIKLSKVDS 360 Query: 360 LDEKAPGFGPIAGLLPLIHFDRKLLLEIMTRNGEEVDVQGLEQTAERAEYWIRHWMPQKL 419 ++ P F +AG LPL++FD +L I+ + + + + RAEYWIR++ +KL Sbjct: 361 EFKEYPKFNQVAGTLPLLNFDSTILQGILKKIDRSYALDEMRAISNRAEYWIRNFQSEKL 420 Query: 420 VTVNTRRNNSLYETLEAHEQEWLAAFCRLLRTGGMEDEPLMKAIYAICYDEDKKSMKNNQ 479 + VN +N Y TL+ ++EWL C++LR+ + LM+ +YAIC+ E+KK MK NQ Sbjct: 421 IAVNQEKNKEFYYTLDERQKEWLVEVCKILRSNK-DYSNLMEQLYAICHHENKKIMKENQ 479 Query: 480 KRLFTLIYQLVLGQETGPRIPVLIQAVGIEKLLALLDF 517 K+LFT+IY+L++ Q GPRIP+LI VGIEK + LLDF Sbjct: 480 KQLFTIIYKLIMNQSNGPRIPLLIHVVGIEKFITLLDF 517 >C2YS39.1 C2YS39_BACCE Lysyl-tRNA synthetase Length = 517 Score = 633 bits (1632), Expect = e-179, Method: Compositional matrix adjust. Identities = 290/518 (55%), Positives = 379/518 (73%), Gaps = 2/518 (0%) Query: 1 MHWSEKIANRLIESHPEQQTFVCASGISPSGHIHIGNFREIVTTYFVSRALEKAGKQVRF 60 MHW+ ++A+ LI HP ++TFVCASGISPSG +HIGNFREI+TTYFV RAL+ GK+ RF Sbjct: 1 MHWAYEVAHELIRKHPNKETFVCASGISPSGSVHIGNFREIITTYFVVRALQDLGKKTRF 60 Query: 61 IFSWDDFDRFRKVPLQVDPSFEQYIGRPYTQVPCPYGCHTSYAEHFEQEFEEALQSFGIR 120 IFSWDD+DRFRKVP +DPSF +YIG PY +P PY CH SYAEHFE+EFE++LQ+FGI Sbjct: 61 IFSWDDYDRFRKVPKNIDPSFAKYIGMPYCDIPDPYDCHNSYAEHFEKEFEKSLQAFGIE 120 Query: 121 PEFIYQSKEYQSRRYAPGILHALRSRGEIYDILMSFKTGEASEADRAAFYPVSIYCGDCG 180 EFIYQ EY+SRRY I +L R EIYDILMSFKTGE SE +R +FYPV++YC CG Sbjct: 121 VEFIYQHAEYRSRRYNENIFESLYKRKEIYDILMSFKTGECSEEERESFYPVTLYCEKCG 180 Query: 181 KDSTTVHSFDEDKELLTYSCTCGHHSSLSIPEADNIKLQWKIDWPMRWNLEQVLFEPGGR 240 KD+TT+ FDE + + Y C CG+ + LS+ + +KL WKIDWPMRW +E V+FEPGGR Sbjct: 181 KDATTITHFDEVLKSVQYECVCGNQNELSVLNTNKMKLNWKIDWPMRWMIEDVIFEPGGR 240 Query: 241 DHSSETGSYNVASVISERIFGSAPPMYEPYEFIRIKGSFAKMSSSSGNNYTPDDLLKVYA 300 DHSSETGSYNV+ I++ IF P Y Y+FI IKG KMSSSSGN+ TP +LLKVY Sbjct: 241 DHSSETGSYNVSKEIAKEIFNREAPHYVAYDFIGIKGHHEKMSSSSGNSITPSELLKVYL 300 Query: 301 PENILFLFAKYQPGAAFHIGMDEDVLRNYTEFERYRKAYGSLTL-TDDIRAALELSLIHP 359 PE ILF+FAKY+P AAFHIG+DEDV+RNYTE+ER + +Y + TL +D+ A++LS + Sbjct: 301 PEVILFMFAKYRPNAAFHIGLDEDVIRNYTEYERLKDSYENKTLKNEDLFEAIKLSRVDS 360 Query: 360 LDEKAPGFGPIAGLLPLIHFDRKLLLEIMTRNGEEVDVQGLEQTAERAEYWIRHWMPQKL 419 ++ P F +AG LPL++FD +L I+ + + + RAEYWIR++ +KL Sbjct: 361 RIKEYPKFNQVAGTLPLLNFDSSILQGILEKIDRSYTLDAMIAICNRAEYWIRNFQSEKL 420 Query: 420 VTVNTRRNNSLYETLEAHEQEWLAAFCRLLRTGGMEDEPLMKAIYAICYDEDKKSMKNNQ 479 + VN RN Y TL+ ++EWL C++LR+ + M+ +Y+IC+ E+KK MK NQ Sbjct: 421 IAVNLERNTEFYNTLDERQKEWLVEVCKILRSNK-DYSNFMENLYSICHHENKKIMKENQ 479 Query: 480 KRLFTLIYQLVLGQETGPRIPVLIQAVGIEKLLALLDF 517 K+LF +IY+L++ Q +GPRIP+LI VG+EK +ALLDF Sbjct: 480 KQLFLIIYRLIMNQSSGPRIPLLIHVVGVEKFIALLDF 517 >B7H5N0.1 B7H5N0_BACC4 Lysyl-tRNA synthetase EC=6.1.1.6 Length = 517 Score = 630 bits (1625), Expect = e-178, Method: Compositional matrix adjust. Identities = 292/519 (56%), Positives = 382/519 (73%), Gaps = 4/519 (0%) Query: 1 MHWSEKIANRLIESHPEQQTFVCASGISPSGHIHIGNFREIVTTYFVSRALEKAGKQVRF 60 MHW+ ++A+ LI HP ++TFVCASGISPSG +HIGNFREIVTTYFV RAL+ GK+ RF Sbjct: 1 MHWAYEVAHELIRKHPNKETFVCASGISPSGSVHIGNFREIVTTYFVVRALQDLGKKTRF 60 Query: 61 IFSWDDFDRFRKVPLQVDPSFEQYIGRPYTQVPCPYGCHTSYAEHFEQEFEEALQSFGIR 120 IFSWDD+DRFRKVP +DPSF +YIG PY +P PYGC+ SYAEHFE+EFE++LQ+FGI Sbjct: 61 IFSWDDYDRFRKVPQNIDPSFAKYIGMPYCDIPDPYGCYNSYAEHFEKEFEKSLQAFGIE 120 Query: 121 PEFIYQSKEYQSRRYAPGILHALRSRGEIYDILMSFKTGEASEADRAAFYPVSIYCGDCG 180 EFIYQ EY+S RY IL AL R EIYDILM FKTGE SE +R +FYP ++YC CG Sbjct: 121 VEFIYQHDEYRSGRYNENILEALYKRKEIYDILMDFKTGECSEKERESFYPATLYCERCG 180 Query: 181 KDSTTVHSFDEDKELLTYSCTCGHHSSLSIPEADNIKLQWKIDWPMRWNLEQVLFEPGGR 240 KD+TT+ F+E +++ Y C CG+ LS+ + +KL WKIDWPMRW +E V+FEPGGR Sbjct: 181 KDTTTITHFNEVLKIVRYECECGNEKELSVLNTNKMKLNWKIDWPMRWMIEDVVFEPGGR 240 Query: 241 DHSSETGSYNVASVISERIFGSAPPMYEPYEFIRIKGSFAKMSSSSGNNYTPDDLLKVYA 300 DHSSETGSYNV+ I+ +IF P Y Y+FI IKG+ KMSSSSGN+ TP DLLKVY Sbjct: 241 DHSSETGSYNVSKEIARKIFNCEAPHYVAYDFIGIKGNHEKMSSSSGNSITPSDLLKVYI 300 Query: 301 PENILFLFAKYQPGAAFHIGMDEDVLRNYTEFERYRKAYGSLTL-TDDIRAALELSLIHP 359 PE ILF+FAKY+PGAAFHIG+DEDV+RNYTE+ER + +Y + L +D+ A++LS++ Sbjct: 301 PEVILFMFAKYKPGAAFHIGLDEDVIRNYTEYERLKDSYENKMLKNEDLFDAIKLSIVDS 360 Query: 360 LDEKAPGFGPIAGLLPLIHFDRKLLLEIMTRNGEEVDVQGLEQTAERAEYWIRHWMPQKL 419 ++ P F +AG LPL++FD +L I+ + + + + RAEYWIR++ +KL Sbjct: 361 EFKEYPRFNQVAGTLPLLNFDSTILQGILKKIDRSYALDEMRVISNRAEYWIRNFQSEKL 420 Query: 420 VTVNTRRNNSLYETLEAHEQEWLAAFCRLLRTGGMED-EPLMKAIYAICYDEDKKSMKNN 478 + VN +N Y TL+ ++EWL C++LR+ ++D LM+ +Y IC+ E+KK MK N Sbjct: 421 IAVNQEKNAEFYYTLDERQKEWLVEVCKILRS--IKDYSNLMEQLYTICHHENKKIMKEN 478 Query: 479 QKRLFTLIYQLVLGQETGPRIPVLIQAVGIEKLLALLDF 517 QK+LFT+IY+L++ Q GPRIP+LI VGIEK + LLDF Sbjct: 479 QKQLFTIIYKLIMNQSNGPRIPLLIHVVGIEKFITLLDF 517 >C3FKN8.1 C3FKN8_BACTB Lysyl-tRNA synthetase Length = 517 Score = 629 bits (1622), Expect = e-178, Method: Compositional matrix adjust. Identities = 291/519 (56%), Positives = 382/519 (73%), Gaps = 4/519 (0%) Query: 1 MHWSEKIANRLIESHPEQQTFVCASGISPSGHIHIGNFREIVTTYFVSRALEKAGKQVRF 60 MHW+ ++A+ LI HP ++TFVCASGISPSG +HIGNFREIVTTYFV RAL+ GK+ RF Sbjct: 1 MHWAYEVAHELIRKHPNKETFVCASGISPSGSVHIGNFREIVTTYFVVRALQDLGKKTRF 60 Query: 61 IFSWDDFDRFRKVPLQVDPSFEQYIGRPYTQVPCPYGCHTSYAEHFEQEFEEALQSFGIR 120 IFSWDD+DRFRKVP +DPSF +Y+G PY +P PYGCH SYAEHFE+EFE++LQ+FGI Sbjct: 61 IFSWDDYDRFRKVPKNIDPSFAKYMGMPYCDIPDPYGCHNSYAEHFEKEFEKSLQAFGIE 120 Query: 121 PEFIYQSKEYQSRRYAPGILHALRSRGEIYDILMSFKTGEASEADRAAFYPVSIYCGDCG 180 EFIYQ EY+S RY IL AL R EIYDILM FKTGE SE +R +FYP ++YC CG Sbjct: 121 VEFIYQHDEYRSGRYNENILEALYKRKEIYDILMDFKTGECSEKERESFYPATLYCERCG 180 Query: 181 KDSTTVHSFDEDKELLTYSCTCGHHSSLSIPEADNIKLQWKIDWPMRWNLEQVLFEPGGR 240 KD+TT+ F+E +++ Y C CG+ LS+ + +KL WKIDWPMRW +E V+FEPGGR Sbjct: 181 KDTTTITHFNEVLKIVRYECECGNEKELSVLNTNKMKLNWKIDWPMRWMIEDVVFEPGGR 240 Query: 241 DHSSETGSYNVASVISERIFGSAPPMYEPYEFIRIKGSFAKMSSSSGNNYTPDDLLKVYA 300 DHSSETGSYNV+ I+ +IF P Y Y+FI IKG+ KMSSSSGN+ TP DLLKVY Sbjct: 241 DHSSETGSYNVSKEIARKIFNCEAPHYVAYDFIGIKGNHEKMSSSSGNSITPSDLLKVYI 300 Query: 301 PENILFLFAKYQPGAAFHIGMDEDVLRNYTEFERYRKAYGSLTL-TDDIRAALELSLIHP 359 PE ILF+FAKY+PGAAFHIG+ EDV+RNYTE+ER + +Y + L +D+ A++LS++ Sbjct: 301 PEVILFMFAKYKPGAAFHIGLYEDVIRNYTEYERLKDSYENKMLKNEDLFDAIKLSIVDS 360 Query: 360 LDEKAPGFGPIAGLLPLIHFDRKLLLEIMTRNGEEVDVQGLEQTAERAEYWIRHWMPQKL 419 + ++ P F +AG LPL++FD +L I+ + + + + RAEYWIR++ +KL Sbjct: 361 VFKEYPRFNQVAGTLPLLNFDSTILQGILKKIDRSYALDEMRVISNRAEYWIRNFQSEKL 420 Query: 420 VTVNTRRNNSLYETLEAHEQEWLAAFCRLLRTGGMED-EPLMKAIYAICYDEDKKSMKNN 478 + VN +N Y TL+ ++EWL C++LR+ ++D LM+ +Y IC+ E+KK MK N Sbjct: 421 IAVNQEKNAEFYYTLDERQKEWLVEVCKILRS--IKDYSNLMEQLYTICHHENKKIMKEN 478 Query: 479 QKRLFTLIYQLVLGQETGPRIPVLIQAVGIEKLLALLDF 517 QK+LFT+IY+L++ Q GPRIP+LI VGIEK + LLDF Sbjct: 479 QKQLFTIIYKLIMNQSNGPRIPLLIHVVGIEKFITLLDF 517 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,252,918,005 Number of extensions: 140301167 Number of successful extensions: 300380 Number of sequences better than 1.0e-05: 312 Number of HSP's gapped: 299316 Number of HSP's successfully gapped: 315 Length of query: 517 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 377 Effective length of database: 1,741,754,103 Effective search space: 656641296831 Effective search space used: 656641296831 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)