BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0006.p (294 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5HNH9.1 B5HNH9_9ACTO NAD-dependent epimerase/dehydratase 308 3e-82 Q08YE3.1 Q08YE3_STIAU NAD-dependent epimerase/dehydratase 303 1e-80 B8J5P5.1 B8J5P5_ANAD2 NAD-dependent epimerase/dehydratase 295 3e-78 Q2K6Y6.1 Q2K6Y6_RHIEC Putative nucleoside-diphosphate-sugar epim... 293 1e-77 A8L0F6.1 A8L0F6_FRASN NAD-dependent epimerase/dehydratase 291 6e-77 >B5HNH9.1 B5HNH9_9ACTO NAD-dependent epimerase/dehydratase Length = 298 Score = 308 bits (790), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 162/291 (55%), Positives = 201/291 (69%), Gaps = 2/291 (0%) Query: 1 MRVFVTGATGFIGSAVVKELIHAGHEVVGLARSDKAAASLHAMGAEALRGSLGDPNGLRS 60 MRVFVTGATGF+GSAV +EL+HAGHEVVGLARSD++ +L A GAE RG L D + LR Sbjct: 1 MRVFVTGATGFVGSAVTRELLHAGHEVVGLARSDRSVEALKAAGAEVHRGDLTDLDSLRR 60 Query: 61 GAAEADGVIHLAFDNSFTDFAGAIADDLQAIEVIGDVLAGSGKPIVMTSVTTMVPSLGRP 120 GA ADGV HLAF + FT+ + DL+AIE +G+VL G+GKP V+TS P GR Sbjct: 61 GAEAADGVAHLAFIHDFTNLTASGEADLKAIEAVGEVLEGTGKPFVVTSGLLTAP--GRV 118 Query: 121 ATEDDQAQDGSPGLPRSIAENMAIALAARDVRSSVVRLAPCVHDATSQGFATLLAELARQ 180 TE+D A GSPG R +E +ALA R V +SV+RL P VH GF + + AR Sbjct: 119 GTEEDPADPGSPGHHRLASEVATLALAERGVSASVLRLPPSVHGEGDLGFVPMFIDHARD 178 Query: 181 KGVSAYVGDGANHWPALHRLDAAKLYRLALESAPAGSRLHGAGEEAIPFRNIAEAIGQRL 240 KGVSA VGDG+NHW A+HRLDAA+L+RLALE+APAG RLH G+E +PF IAE IG+ L Sbjct: 179 KGVSAMVGDGSNHWAAVHRLDAARLFRLALEAAPAGVRLHAIGDEGVPFHEIAETIGRGL 238 Query: 241 NLPVSSISPEAAEEHFGWLAPFVLMDNPTSNVWTREKLGWEPAEPTLVADI 291 ++PV+S++PE A +HFGWL F +D S TRE LGW P L+ D+ Sbjct: 239 DVPVASVAPEEATDHFGWLGRFAAIDMRASAARTRELLGWRPEHAALIPDL 289 >Q08YE3.1 Q08YE3_STIAU NAD-dependent epimerase/dehydratase Length = 310 Score = 303 bits (776), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 174/297 (58%), Positives = 209/297 (70%), Gaps = 10/297 (3%) Query: 1 MRVFVTGATGFIGSAVVKELIHAGHEVVGLARSDKAAASLHAMGAEALRGSLGDPNGLRS 60 MRVFVTGA+G IGSA+V EL+ AGH+V+GLARSD +AA+L + G E RG L D +GLR Sbjct: 1 MRVFVTGASGHIGSALVPELLAAGHKVIGLARSDTSAAALSSAGVEVRRGDLDDLDGLRQ 60 Query: 61 GAAEADGVIHLAF--DNSFT-DFAGAIADDLQAIEVIGDVLAGSGKPIVMTSVTTMVP-- 115 AA ADGVIHLAF D +FT D+AGA A + +A+E IG L GSGKP V T+ T + Sbjct: 61 AAAAADGVIHLAFKHDVAFTGDYAGAAAAEFRAVEAIGAALEGSGKPFVSTAGTLALALG 120 Query: 116 SLGRPATEDDQAQDGSPGLPRSIAENMAIALAARDVRSSVVRLAPCVHDATSQ-GFATLL 174 GR TEDD + G PR +EN IAL R VRSSV+RLAP VH + GF L Sbjct: 121 GSGRMGTEDDAREGG----PRIDSENAVIALGKRGVRSSVIRLAPTVHSSLDHHGFVPSL 176 Query: 175 AELARQKGVSAYVGDGANHWPALHRLDAAKLYRLALESAPAGSRLHGAGEEAIPFRNIAE 234 +AR+ G +AYVGDGAN WP +H LDA +LYRLALE+APAGSRLHGA EE IPFR+IAE Sbjct: 177 ITMARKNGFAAYVGDGANRWPTVHTLDATRLYRLALETAPAGSRLHGAAEEGIPFRDIAE 236 Query: 235 AIGQRLNLPVSSISPEAAEEHFGWLAPFVLMDNPTSNVWTREKLGWEPAEPTLVADI 291 AIG+ L LP SIS E A +H G+LA F +DNPTS+V TRE L W+P P L+AD+ Sbjct: 237 AIGRGLGLPAVSISAEDAGKHLGFLAMFAQLDNPTSSVRTRELLRWQPTHPGLLADL 293 >B8J5P5.1 B8J5P5_ANAD2 NAD-dependent epimerase/dehydratase Length = 294 Score = 295 bits (755), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 165/291 (56%), Positives = 203/291 (69%), Gaps = 3/291 (1%) Query: 1 MRVFVTGATGFIGSAVVKELIHAGHEVVGLARSDKAAASLHAMGAEALRGSLGDPNGLRS 60 MRVFVTGA+GFIGSAVV EL+ AGH+VVGLARSD +A +L A GAE RG L DP+ LR+ Sbjct: 1 MRVFVTGASGFIGSAVVPELLAAGHQVVGLARSDTSARALAAAGAEVHRGDLEDPDSLRA 60 Query: 61 GAAEADGVIHLAFDNSFTDFAGAIADDLQAIEVIGDVLAGSGKPIVMTSVTTMVPSLGRP 120 GA ADGVIHL F + F F +I D AIE +G VL GSG+P+V+ S T + + GR Sbjct: 61 GADGADGVIHLGFIHDFERFDASIRSDRNAIETLGAVLEGSGRPLVIASGTLGI-APGRV 119 Query: 121 ATEDDQAQDGSPGLPRSIAENMAIALAARDVRSSVVRLAPCVHDATSQGFATLLAELARQ 180 ATE + G PR +A++L R VRS+ VRLAP VH GF L E+AR+ Sbjct: 120 ATE--LIPFDAKGHPRLANALVALSLKDRGVRSAAVRLAPSVHGEGDHGFVRRLVEIARE 177 Query: 181 KGVSAYVGDGANHWPALHRLDAAKLYRLALESAPAGSRLHGAGEEAIPFRNIAEAIGQRL 240 KGVS Y GDG+N W A+HRLDAA+L+RLALESAPAGS LH GEEA+ R IAEAI +L Sbjct: 178 KGVSGYPGDGSNRWNAVHRLDAARLFRLALESAPAGSVLHAVGEEAVTLRTIAEAIATQL 237 Query: 241 NLPVSSISPEAAEEHFGWLAPFVLMDNPTSNVWTREKLGWEPAEPTLVADI 291 LPV+S++PEAA +HFGWL FV +D P S+ T+E++GW P P L+ DI Sbjct: 238 KLPVASVAPEAASDHFGWLGGFVAVDQPASSALTQERMGWRPTHPGLIQDI 288 >Q2K6Y6.1 Q2K6Y6_RHIEC Putative nucleoside-diphosphate-sugar epimerase dehydratase protein Length = 296 Score = 293 bits (750), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 164/291 (56%), Positives = 200/291 (68%), Gaps = 3/291 (1%) Query: 1 MRVFVTGATGFIGSAVVKELIHAGHEVVGLARSDKAAASLHAMGAEALRGSLGDPNGLRS 60 MRVFVTGATG++GSAVVKELI AGH+V+GLARSDK AA L A GAE RG+L D GL+ Sbjct: 1 MRVFVTGATGWVGSAVVKELIAAGHQVLGLARSDKGAAELAAAGAEVQRGTLNDLEGLKR 60 Query: 61 GAAEADGVIHLAFDNSFTDFAGAIADDLQAIEVIGDVLAGSGKPIVMTSVTTMVPSLGRP 120 GAAEADGVIH AF++ F++FA A D +AIE +G+ L GS +P+++T+ P GR Sbjct: 61 GAAEADGVIHTAFNHDFSNFAENCAADRRAIEALGEALRGSNRPLLVTAGLGHAP--GRI 118 Query: 121 ATEDDQAQDGSPGLPRSIAENMAIALAARDVRSSVVRLAPCVHDATSQGFATLLAELARQ 180 TE D + PR+ +E A+AL AR VR+S VRL P VH GF +L E ARQ Sbjct: 119 GTEKDPPMPTTQTYPRA-SEITAVALVARGVRASTVRLPPSVHGHGDHGFVPILIETARQ 177 Query: 181 KGVSAYVGDGANHWPALHRLDAAKLYRLALESAPAGSRLHGAGEEAIPFRNIAEAIGQRL 240 KG+SAY+GDG N WPA+HR DAA++YRLALE G EE +PFR IAE IG+RL Sbjct: 178 KGLSAYIGDGGNRWPAVHRRDAARVYRLALERGAVGGPFLAVAEEGVPFREIAELIGRRL 237 Query: 241 NLPVSSISPEAAEEHFGWLAPFVLMDNPTSNVWTREKLGWEPAEPTLVADI 291 N+P S+S E A EHFGW A F D PTS+V TR LGW+P E L+ADI Sbjct: 238 NVPAVSLSQEEAAEHFGWFAMFAGFDVPTSSVHTRALLGWQPTEAGLLADI 288 >A8L0F6.1 A8L0F6_FRASN NAD-dependent epimerase/dehydratase Length = 298 Score = 291 bits (745), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 160/292 (54%), Positives = 204/292 (69%), Gaps = 5/292 (1%) Query: 1 MRVFVTGATGFIGSAVVKELIHAGHEVVGLARSDKAAASLHAMGAEALRGSLGDPNGLRS 60 MR+FVTGA+G+IGSAV+ EL AGH+VVGLARSD AAA++ A+GAE LRG LGDP+ LR+ Sbjct: 1 MRIFVTGASGWIGSAVIPELRDAGHQVVGLARSDTAAATVSALGAEVLRGGLGDPDSLRA 60 Query: 61 GAAEADGVIHLAFDNSFTDFAGAIADDLQAIEVIGDVLAGSGKPIVMTSVTTMVPSLGRP 120 GAA +DGV+HLA+ + F+ A A DLQAIE +G L GSG+P+++ S T+ ++GR Sbjct: 61 GAAGSDGVVHLAYVHDFSRIAQAARTDLQAIEALGAALEGSGRPLLIAS-GTLGLAVGRV 119 Query: 121 ATEDDQAQDGSPGL-PRSIAENMAIALAARDVRSSVVRLAPCVHDATSQGFATLLAELAR 179 TE D A P + PR + + A+A A R VRSSVVR AP VH A GF +L +AR Sbjct: 120 GTERDSAD---PSVHPRIASAHAALAYAERGVRSSVVRFAPTVHGAGDHGFVAVLVGIAR 176 Query: 180 QKGVSAYVGDGANHWPALHRLDAAKLYRLALESAPAGSRLHGAGEEAIPFRNIAEAIGQR 239 ++GVS Y+GDG N WPA+HRLDAA L LA++ APAGS LH EE +P R +AEAIG+ Sbjct: 177 ERGVSGYIGDGENRWPAVHRLDAAHLVSLAVDGAPAGSVLHAVAEEGVPIRAVAEAIGRG 236 Query: 240 LNLPVSSISPEAAEEHFGWLAPFVLMDNPTSNVWTREKLGWEPAEPTLVADI 291 L +PV S+ E A +HFGWLAPF+ D P SN T E L W+P LV D+ Sbjct: 237 LGVPVVSVPAERAGDHFGWLAPFLAADCPASNHLTSELLSWKPTRQGLVEDL 288 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,767,249,422 Number of extensions: 74698639 Number of successful extensions: 313133 Number of sequences better than 1.0e-05: 319 Number of HSP's gapped: 313365 Number of HSP's successfully gapped: 321 Length of query: 294 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 160 Effective length of database: 1,798,280,193 Effective search space: 287724830880 Effective search space used: 287724830880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)